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Yun, Seung Pil,Han, Yong-Seok,Lee, Jun Hee,Yoon, Yeo Min,Yun, Chul Won,Rhee, Peter,Lee, Sang Hun SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol.16 No.5
<P>Cellular prion protein (PrP<SUP>C</SUP>) can replace other pivotal molecules due to its interaction with several partners in performing a variety of important biological functions that may differ between embryonic and mature stem cells. Recent studies have revealed major advances in elucidating the putative role of PrP<SUP>C</SUP> in the regulation of stem cells and its application in stem cell therapy. What is special about PrP<SUP>C</SUP> is that its expression may be regulated by hypoxia-inducible factor (HIF)-1α, which is the transcriptional factor of cellular response to hypoxia. Hypoxic conditions have been known to drive cellular responses that can enhance cell survival, differentiation and angiogenesis through adaptive processes. Our group recently reported hypoxia-enhanced vascular repair of endothelial colony-forming cells on ischemic injury. Hypoxia-induced AKT/signal transducer and activator of transcription 3 phosphorylation eventually increases neovasculogenesis. In stem cell biology, hypoxia promotes the expression of growth factors. According to other studies, aspects of tissue regeneration and cell function are influenced by hypoxia, which serves an essential role in stem cell HIF-1α signaling. All these data suggest the possibility that hypoxia-mediated PrP<SUP>C</SUP> serves an important role in angiogenesis. Therefore, the present review summarizes the characteristics of PrP<SUP>C</SUP>, which is produced by HIF-1α in hypoxia, as it relates to angiogenesis.</P>
윤제필 ( Je Pil Yun ),박종민 ( Jong Min Park ),윤문식 ( Moon Sik Yun ),김성용 ( Sung Yong Kim ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.4
Objective : There were few case reports on the treatment of Herpes Zoster Myelitis by oriental medicine. We experienced one case of a female patient, 33 ages, who had complants; severe neck pain and motor, sensory impairment and headache. Methods : We used acupuncture, negative, physical therapy, chuna, herbal medication. Results and Conclusions :After oriental medical treatments, clinical improvement of the neurologic impairment was noted. We expected that therapeutic value of treatment of Herpes Zoster Myelitis in the oriental medicine will be higher if more clinical studies and researches are accomplished.
Park, Jae-Won,Shin, Yun Kyung,Choen, Yong-Pil 한국발생생물학회 2014 발생과 생식 Vol.18 No.3
Adaptive development of early stage embryo is well established and recently it is explored that the mammalian embryos also have adaptive ability to the stressful environment. However, the mechanisms are largely unknown. In this study, to evaluate the possible role of aquaporin in early embryo developmental adaptation, the expression of aquaporin (AQP) 5 gene which is detected during early development were examined by the environmental condition. To compare expression patterns between in vivo and in vitro, we conducted quantitative RT-PCR and analyzed localization of the AQP5 by whole mount immunofluorescence. At in vivo condition, Aqp5 expressed in oocyte and in all the stages of preimplantation embryo. It showed peak at 2-cell stage and decreased continuously until morula stage. At in vitro condition, Aqp5 expression pattern was similar with in vivo embryos. It expressed both at embryonic genome activation phase and second mid-preimplantation gene activation phase, but the fold changes were modified between in vivo embryos and in vitro embryos. During in vivo development, AQP5 was mainly localized in apical membrane of blastomeres of 4-cell and 8-cell stage embryos, and then it was localized in cytoplasm. However, the main localization area of AQP5 was dramatically shifted after 8-cell stage from cytoplasm to nucleus by in vitro development. Those results explore the modification of Aqp5 expression levels and location of its final products by in vitro culture. It suggests that expression of Aqp5 and the roles of AQP5 in homeostasis can be modulated by in vitro culture, and that early stage embryos can develop successfully by themselves adapting to their condition through modulation of the specific gene expression and localization.
Yun, Seung Pil,Han, Yong-Seok,Lee, Jun Hee,Kim, Sang Min,Lee, Sang Hun The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.4
p-Cresol, found at high concentrations in the serum of chronic kidney failure patients, is known to cause cell senescence and other complications in different parts of the body. p-Cresol is thought to mediate cytotoxic effects through the induction of autophagy response. However, toxic effects of p-cresol on mesenchymal stem cells have not been elucidated. Thus, we aimed to investigate whether p-cresol induces senescence of mesenchymal stem cells, and whether melatonin can ameliorate abnormal autophagy response caused by p-cresol. We found that p-cresol concentration-dependently reduced proliferation of mesenchymal stem cells. Pretreatment with melatonin prevented pro-senescence effects of p-cresol on mesenchymal stem cells. We found that by inducing phosphorylation of Akt and activating the Akt signaling pathway, melatonin enhanced catalase activity and thereby inhibited the accumulation of reactive oxygen species induced by p-cresol in mesenchymal stem cells, ultimately preventing abnormal activation of autophagy. Furthermore, preincubation with melatonin counteracted other pro-senescence changes caused by p-cresol, such as the increase in total 5'-AMP-activated protein kinase expression and decrease in the level of phosphorylated mechanistic target of rapamycin. Ultimately, we discovered that melatonin restored the expression of senescence marker protein 30, which is normally suppressed because of the induction of the autophagy pathway in chronic kidney failure patients by p-cresol. Our findings suggest that stem cell senescence in patients with chronic kidney failure could be potentially rescued by the administration of melatonin, which grants this hormone a novel therapeutic role.
Detection of Scratch Defects on Slab Surface
Yong-Ju Jeon,Doo-chul Choi,Jong Pil Yun,Changhyun Park,Sang Woo Kim 제어로봇시스템학회 2011 제어로봇시스템학회 국제학술대회 논문집 Vol.2011 No.10
Non-destructive automatic inspection systems are in high demand in the steel industry, for controlling the quality and increasing the productivity of steel products. In this study, we developed an algorithm for detecting scratch defects on slabs by using image-processing techniques. To reduce the effect of lighting condition and amplify the features of the defects, we used a Gabor filter and carried out edge-pair detection. The histogram-based and gradient texture features, and support vector machine are used to detect the scratch defects. Experimental results proved the effectiveness of the proposed algorithm.
Yun, Young-Pil,Lee, Jae Yong,Jeong, Won Jae,Park, Kyeongsoon,Kim, Hak-Jun,Song, Jae-Jun,Kim, Sung Eun,Song, Hae-Ryong Hindawi Publishing Corporation 2015 BioMed research international Vol.2015 No.-
<P>The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the <I>γ</I>-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the <I>γ</I>-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP-2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and the mRNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells.</P>