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Hyunsu Kim,Kyung‑Wan Baek,고은지,Nguyen Thanh Luan,Yunjin Lim,Heyong Jin Roh,Nameun Kim,Do‑Hyung Kim,Ahran Kim,Yung Hyun Choi,Suhkmann Kim,Heui‑Soo Kim,Mee Sun Ock,차희재 한국유전학회 2020 Genes & Genomics Vol.42 No.7
Background Viral hemorrhagic septicemia (VHS) is a serious viral disease that infects the olive flounder in South Korea. The Korean aquaculture industry experienced an economic loss caused by the high infectivity and mortality. Objective This study aimed to evaluate the infection density of VHSV in various organs of the olive flounder including spleen, liver, kidney, stomach, esophagus, intestine, gill, muscle, heart, and brain. Olive flounders were collected from a local fish farm and injected subcutaneously with 106 PFU/fish. Methods Each 15 fish were sampled at 0, 3, and 7 days post challenge (dpc), respectively, to perform quantitative analysis of VHSV using SYBR-green based real-time PCR in various tissues including spleen, liver, head-kidney, body-kidney, muscle, esophagus, stomach, intestine, gill, and brain. Results Organs infected with VHSV were obtained after 3 and 7 days. Each organs were examined for viral infection using real-time PCR. The data obtained from this experiment revealed copy numbers higher than 10 copies per 100 ng cDNA in the spleen (15.26 ± 3.11 copies/100 ng of cDNA), muscle (11.24 ± 2.25 copies), and gill (14.23 ± 6.26 copies), but lower in liver, head-kidney, body-kidney, esophagus, brain and stomach. Conclusion The present study, together with previous data, demonstrated that the gill, spleen, and muscle are the major target organs of VHSV in olive flounder. Therefore, central monitoring of spleen, gill and muscle should be considered and might be necessary if anti-VHSV treatment is to be successful in infected olive flounder.
Abnormal Sperm Development in pcd^(3J)-/- Mice: the Importance of Agtpbp1 in Spermatogenesis
Nameun Kim,Rui Xiao,Hojun Choi,Haiin Jo,Jin-Hoi Kim,엄상준,박찬규 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.1
Homozygous Purkinje cell degeneration (pcd) mutant males exhibit abnormal sperm development. Microscopic examination of the testes from pcd^(3J)-/-mice at postnatal days 12, 15, 18 and 60 revealed histological differences, in comparison to wild-type mice, which were evident by day 18. Greatly reduced numbers of spermatocytes and spermatids were found in the adult testes, and apoptotic cells were identified among the differentiating germ cells after day 15. Our immunohistological analysis using an antihuman AGTPBP1 antibody showed that AGTPBP1 was expressed in spermatogenic cells between late stage primary spermatocytes and round spermatids. A global gene expression analysis from the testes of pcd^(3J)-/-mice showed that expression of cyclin B3 and de-ubiquitinating enzymes USP2 and USP9y was altered by >1.5-fold compared to the expression levels in the wild-type. Our results suggest that the pcd mutant mice have defects in spermatogenesis that begin with the pachytene spermatocyte stage and continue through subsequent stages. Thus, Agtpbp1, the gene responsible for the pcd phenotype, plays an important role in spermatogenesis and is important for survival of germ cells at spermatocytes stage onward.
19세기 후반 동아시아 국제질서의 변화와 제국 일본-조선 ‘속방 자주’ 논쟁을 중심으로
김남은 ( Kim Nameun ) 한림대학교 일본학연구소 2022 翰林日本學 Vol.- No.41
본고는 근대 일본의 팽창 과정에 초점을 맞추어 조선의 ‘속방’과 ‘자주’에 대한 일본의 대응이 동아시아 국제질서를 어떻게 변화시켜 나가는지를 고찰하고 있다. 특히 조선 ‘속방 자주’에 대한 일본의 전략을 임오군란 사후처리와 갑신정변 사후처리를 위한 협상 속에서 포착하여 그 성격을 규명하고자 했다. 일본의 근대국제체제로의 이행은 조선과 중국과의 조공책봉관계를 타파하는 과정에 다름 아니었으며, 이는 곧 조선과 중국의 관계 속에서 일본을 중심으로 하는 새로운 지역질서를 구축하기 위한 과정이기도 했다. 그 과정에서 쟁점이 된 조선의 ‘속방’과 ‘자주’ 논쟁은 중국과 일본이 전통적인 조공관계를 상투적으로 이용했던 사례 중 하나일 뿐이며, 양국의 궁극적인 목적은 조선에 대한 영향력을 확보하려는 데 있었다. 그리고 이러한 일본의 근대국제체제로의 능동적인 대응은 일본이 제국주의 국가의 반열에 오르는 것을 꿈꾸게 되는 효시로 작용하게 된다. The purpose of this paper is to examine how Japan's response to Joseon's dependent and independent issues has changed the international order in East issues has changed the international order in East Asia. Japan's transition to modern times was a process of breaking down the traditional relationship between Joseon and China. At the same time, Japan sought to establish a new regional order centered on its own country. The issue of dependent and independent that became an issue in the process is only one of the cases in which Japan and China used traditional tribute relations. The ultimate purpose of Japan and China was to secure influence over Joseon. This paper aims to identify the nature of Japan's response to dependent and independent in negotiations for the post-treatment of the Im-O Military Revolt and the Gapsin Coup.
( Yoonhang Lee ),( Nameun Kim ),( Ju-yeop Lee ),( Hyoyeong Kang ),( Minji Sung ),( Young-bin Yu ),( Kyunghoi Kim ),( Jae-young Je ),( Hyun-woo Kim ),( Ju-chan Kang ),( Do-hyung Kim ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2
This is the first report describing acute mass mortality occurred in juvenile olive flounder (Paralichthys olivaceus) caused by gas bubble disease (GBD). A total of 610 fish (average weight = 35 g), which were more than half of the fish acclimated at 17℃ in an aquarium, were killed within two days of acclimation. The dead and moribund fish showed excessively opened opercula and mouths, and occasionally, severe exophthalmia. Through microscopic observation, numerous gas emboli were found in the gills of the dead and live fish, while the fish were not infected with any microbial pathogens. The dissolved oxygen (DO) saturation level of the rearing water and seawater nearby the facility reached 145% and 286%, respectively, whereas other water quality parameters (such as salinity, pH, and chemical oxygen demand) were normal. The extreme saturation rate of seawater in the shore nearby seemed to be due to an enormous algal bloom that occurred there. Through molecular identification based on 18S rDNA sequences, the most dominant algal species was most closely related to Ulva californica (99.87% sequence identity) followed by U. prolifera, U. linza, and U. curvata (99.81%). Therefore, it can be concluded that supersaturated seawater due to mass algal bloom caused gas bubble disease in the olive flounder, leading to mass mortality. After technical adjustment, such as increased aeration, lowered water circulation rate, and inlet water filtration using micro-pore carbon filters, the DO level became normal, no further mortality occurred and the status of the fish was stabilized.
( Hyeongjin Roh ),( Bo Seong Kim ),( Ahran Kim ),( Nameun Kim ),( Mu Kun Lee ),( Chan-il Park ),( Do-hyung Kim ) 한국어병학회 2020 한국어병학회지 Vol.33 No.1
In recent years, global warming is causing dramatic environmental changes and deterioration, such as hypoxia, leading to reduced survival rate and growth performance of farmed aquatic animals. Hence, understanding systemic immuno-physiological changes in fish under environmental stress might be important to maximize aquaculture production. In this study, we investigated physiological changes in rainbow trout exposed to hypoxic stress by monitoring changes in blood chemistry, leukocyte population, and expression levels of related cytokine genes. Hematological and serological factors were evaluated in blood obtained from rainbow trout sampled at a dissolved level of 4.6 mg O<sub>2</sub> L<sup>-1</sup> and 2.1 mg O<sub>2</sub> L<sup>-1</sup>. Blood and head kidney tissue obtained at each sampling time point were used to determine erythrocyte size, leukocyte population, and cytokine gene expression. The level of LDH and GPT in fish under progressive hypoxia were significantly increased in plasma. Likewise, the (Granulocyte + Macrophage)/lymphocyte ratio (%) of fish exposed to hypoxia was significantly lower than that in fish in the control group. Such changes might be due to the rapid movement of lymphocytes in fish exposed to acute hypoxia. In this study, significant up-regulation in expression levels of IL-1β and IL-6 gene appeared to be involved in the redistribution of leukocytes in rainbow trout. This is the first study to demonstrate the involvement of cytokines in leukocyte trafficking in fish exposed to hypoxia. It will help us understand systemic physiological changes and mechanisms involved in teleost under hypoxic stress.