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RISS 인기검색어
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- 저자 : ( Hyeongjin Roh ) (검색결과 3 건)
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( Hyeongjin Roh ),( Bo Seong Kim ),( Ahran Kim ),( Nameun Kim ),( Mu Kun Lee ),( Chan-il Park ),( Do-hyung Kim ) 한국어병학회 2020 한국어병학회지 Vol.33 No.1
In recent years, global warming is causing dramatic environmental changes and deterioration, such as hypoxia, leading to reduced survival rate and growth performance of farmed aquatic animals. Hence, understanding systemic immuno-physiological changes in fish under environmental stress might be important to maximize aquaculture production. In this study, we investigated physiological changes in rainbow trout exposed to hypoxic stress by monitoring changes in blood chemistry, leukocyte population, and expression levels of related cytokine genes. Hematological and serological factors were evaluated in blood obtained from rainbow trout sampled at a dissolved level of 4.6 mg O<sub>2</sub> L<sup>-1</sup> and 2.1 mg O<sub>2</sub> L<sup>-1</sup>. Blood and head kidney tissue obtained at each sampling time point were used to determine erythrocyte size, leukocyte population, and cytokine gene expression. The level of LDH and GPT in fish under progressive hypoxia were significantly increased in plasma. Likewise, the (Granulocyte + Macrophage)/lymphocyte ratio (%) of fish exposed to hypoxia was significantly lower than that in fish in the control group. Such changes might be due to the rapid movement of lymphocytes in fish exposed to acute hypoxia. In this study, significant up-regulation in expression levels of IL-1β and IL-6 gene appeared to be involved in the redistribution of leukocytes in rainbow trout. This is the first study to demonstrate the involvement of cytokines in leukocyte trafficking in fish exposed to hypoxia. It will help us understand systemic physiological changes and mechanisms involved in teleost under hypoxic stress.
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Differential proteome profile of gill and spleen in three pathogen-infected Paralichthys olivaceus
A-Reum Lee,Hyunsu Kim,Kyung-Yoon Jeon,고은지,Ahran Kim,Nameun Kim,HyeongJin Roh,Yoonhang Lee,Jiyeon Park,Do‑Hyung Kim,Yung Hyun Choi,김석만,김희수,Mee Sun Ock,차희재 한국유전학회 2021 Genes & Genomics Vol.43 No.7
Background Olive founder (Paralichthys olivaceus) is one of the major cultured fsh species in Asia including Korea. However, the mass mortality of olive founder caused by various pathogens leads to huge economic loss. The pathogens that lead to fsh mortality include parasites, bacteria, and viruses that can cause various kinds of diseases. Objective The purpose of this study was to investigate the protein expression patterns in the gills and spleens of olive founder after artifcial infection. We hypothesized that proteomics levels in gills and spleen may be diferentially expressed depending on infectious agents. Methods To investigate the expression pattern of proteins in gills and spleens, olive founders were experimentally infected with VHSV (virus), S. parauberis (bacteria), or M. avidus (pathogenic ciliate). Proteins were extracted from the gills and spleens of infected olive founder. We used 2-DE analysis with LC–MS/MS to investigate proteome changes in infected olive founders. Results The results of the LC–MS/MS analyses showed diferent protein expression profles depending on pathogenic sources and target organs. Proteins related to cytoskeletal structure like keratin, calmodulin and actin were mostly expressed in the infected gills. Proteins involved in the metabolism pathway like glycolysis were expressed mainly in the spleens. The protein profles of S. parauberis and VHSV infection groups had many similarities, but the profle of the M. avidus infection group was greatly diferent in the gill and spleen. Conclusion Our results indicate that measures according to the characteristics of each pathogen are necessary for disease prevention and treatment of farmed fsh.
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