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Moon, Hyoung Joon,Oh, Jin Sik,Na, Woonsung,Yeom, Minjoo,Han, Sang Yoon,Kim, Sung Jae,Park, Bong Kyun,Song, Dae Sub,Kang, Bo Kyu 한국조명·전기설비학회 2016 한국조명·전기설비학회 학술대회논문집 Vol. No.
<P>A pandemic influenza A (H1N1) virus strain was isolated from a pig farm in Korea in December 2009. The strain was propagated in and isolated from both the Madin-Darby canine kidney cell line and embryonated eggs. The partial and complete sequences of the strain were identical to those of A/California/04/2009, with >99% sequence similarity in the HA, NA, M, NS, NP, PA, PB1, and PB2 genes. The isolated strain was inactivated and used to prepare a swine influenza vaccine. This trial vaccine, containing the new isolate that has high sequence similarity with the pandemic influenza A (H1N1) virus, resulted in seroconversion in Guinea pigs and piglets. This strain could therefore be a potential vaccine candidate for swine influenza control in commercial farms.</P>
Min Chull Na,Moon Doo Kim,Joon Hyuk Park,Young-Eun Jung,Duk-Soo Moon,Hyun-Ju Yang,Bung-Nyun Kim,Na Ri Kang 대한소아청소년정신의학회 2021 소아청소년정신의학 Vol.32 No.2
Objectives: Adverse childhood experiences (ACEs) of mothers may negatively affect the mental health of their offspring. Little is known about the intergenerational effect of maternal ACE on post-traumatic stress disorder (PTSD) in the offspring. This study investigated the impact of maternal ACEs on PTSD in the offspring. Methods: A total of 156 mothers with children aged 13–18 years completed the Diagnostic Interview Schedule for Children (DISC) Predictive Scales to determine the presence of psychiatric disorders in their offspring. The subjects completed the ACE questionnaire and the Early Trauma Inventory Self-Report-Short Form. Multivariable logistic regression was used to analyze the relationship between maternal ACEs and PTSD in the offspring. Results: Of the mothers, 23.7% had at least one ACE, and PTSD was reported in 21.8% of the offspring. The offspring of the mothers in the ACE group had a significantly higher rates of traumatic experiences and PTSD than the offspring of the mothers in the no ACE group. Maternal household dysfunction independently predicted offspring PTSD [odds ratio (OR)=3.008, p=0.05), and three or more maternal ACEs were significantly related to PTSD in the offspring (OR=10.613, p=0.025). Conclusion: Maternal ACEs have a significant impact on the risk of traumatic experiences and PTSD in the offspring. These findings suggest the presence of intergenerational transmissions by which maternal ACEs affect the mental health of the offspring.
문준일,나운,백준기 중앙대학교 기술과학연구소 1994 기술과학연구소 논문집 Vol.24 No.-
영상복원의 근본적 문제점인 방대한 계산량을 감소시키는데 신경회로망에 근거한 반복적 영상복원 방식이 효율적인 것으로 알려져 있다. 본 논문에서는 개선된 홉필드 모델을 사용해서 인간의 시각특성(Human Visual System;HVS)을 고려한 적응적인 영상복원방법을 제안하다. 즉, 사람은 평탄한 부분의 잡음보다 윤곽부분의 잡음에 둔감하다는 성질을 이용하여, 윤곽부분을 찾아내어 개선된 홉필드 신경망 모델을 사용해 영상을 복원함에 있어서 적응적으로 윤곽부분에서는 열화요인을 제거하여 선명도를 높이고, 평탄한 부분에서는 잡음을 보다 많이 억제시켜서 인간의 시각특성에 만족되는 영상으로 복원해 내는 것이다. 제안된 방식에 의하여 영상을 복원한 결과 비적응적 방식에 의해서 복원된 영상과 비교할 때 평탄한 부분의 잡음이 효율적으로 감쇄되는 동시에 윤곽부분의 정보는 손상되지 않음을 확인할 수 있었다.
사람 Adenylate Kinase 1의 p-Loop부위에 위치한 Serine^(19) 잔기의 돌연변이에 의한 구조 활정 상관관계
문재희,이한나,류용구,김효준 한양대학교 이학기술연구소 2007 이학기술논문지 Vol.10 No.-
Adenylate kinase (AK)는 모든 세포에 존재하는 에너지대사에 관련하는 효소로서 short form과 long form의 두 종류의 isoform으로 분류된다. 포유류 세포의 세포질에 존재하는 short form은 높은 효소 활성(1,800 U/mg)을 나타내며, 전핵생물이나 미토콘드리아에 존재하는 long form은 매우 낮은 활성(200 U/mg)을 나타낸다. 한편, 박테리아인 Mycobacterium tuberculosis의 AK(AKmt)는 구조적으로는 진핵세포와 같은 short form이지만, 활성은 long form의 전형적인 낮은 활성을 갖고 있는 것으로 밝혀졌다. 본 연구에서 사람의AK1(hAK1)과 AKmt의 효소의 촉매활성에 중요한 부위인 P-loop의 아미노산 서열 중 활성이 높은 isoform과 활성이 낮은 isoform에서 serine(Ser^(19)hAK1)과 alanine(Ala^(11)AKmt)으로 각각의 보존성이 상이하여 이 부분이 효소 활성에 미치는 영향을 분석하였다. 부위지정 돌연변이법을 이용하여 hAK1의 19번째 Ser을 Ala으로 치환시킨 돌연변이체(S19AhAK1)를 작성하여 hAK1 및 AKmt간의 활성 및 기질 친화도를 반응속도론적으로 비교한 결과 S19AhAK1의 활성은 450 U/mg으로 AKmt 수준으로 낮아졌으나, 기질 친화도인 Km 값은 ATP 및 AMP에 대하여 큰 변화를 보이지 않았다. 이로서 P-loop의 serine은 side chain의 polarity를 통해 phosphoryl-transfer의 촉매능을 촉진하고 있음을 확인하였다. Adenylate kinase (AK) is a ubiquitous enzyme which related with the energy metabolism in living cells. AK has two kinds of isoforms: short form and long form. Generally the short form has high enzymatic activity (1800 U/mg) and exists in eukaryotic cytosol, while long form has low activity (200 U/mg) and exists in mitochondria and bacteria. Interestingly, AK from Mycobacterium tuberculosis (AKmt) belongs to the short form isozyme, but catalytic activity is as low as that of bacterial long forms'. To characterize this discrepancy, we shed light on the conserved residues in the p-loop of the enzyme. In the flexible P-loop of AK, serine is conserved in high-activity isozymes and alanine is conserved in low-activity isozymes, respectively. Ser^(19) of high activity hAK1 is replaced by Ala at P-loop in low activity AKmt. We substituted Ala for Ser^(19) in hAK1 by site-directed mutagenesis to get a mutant enzyme, S19AhAK1. Enzyme activity of S19AhAK1 was greatly reduced to 450 U/mg, as expected. However, minor changes in Km values for both of ATP and AMP were detected. In condusion, the serine^(19) in hAKl enhances the phosphoryl-transfer catalysis but not in substrate binding, by utilizing the polarity in p-loop environment.
Na Ri Kang,Young Sook Kwack,Jeong-Kook Song,Moon-Doo Kim,Joon Hyuk Park,Bung-Nyun Kim,Duk-Soo Moon 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.11
Objective Adverse childhood experiences (ACEs) are associated with negative physical and mental health outcomes across the lifespan, but research on intergenerational transmission of maternal ACEs and its impact on the offspring’s mental health problems are limited. The study examines the effects of maternal ACEs on the risk of internalizing or externalizing problems among offspring. Methods There were 450 mother-child dyads. Mothers completed the Adverse Childhood Experiences Questionnaire. The child outcomes included internalizing and externalizing problems assessed by the Korean Child Behavior Checklist (K-CBCL) and Korean Youth Self-Report (K-YSR), depression assessed by the Center for Epidemiological Studies Depression Scale for Children (CES-DC) and anxiety assessed by the Screen for Child Anxiety Related Emotional Disorders (SCARED). Results 36.1% of mothers experienced at least one ACE, and 11.1% experienced three or more ACEs. Cumulative maternal ACEs were associated with internalizing problems, externalizing problems, depression and anxiety in the offspring. Household dysfunction from maternal ACEs was significantly associated with delinquent behavior, anxiety/depression, and somatic complaints in the offspring. Conclusion The findings support the hypothesis that maternal ACEs are related to mental health problems in the offspring. Further research is needed to determine the factors mediating intergenerational transmission as well as intervention strategies to prevent ACEs and mental health problems in the offspring.