응고제가 우유두부의 물성 및 관능적 특성에 미치는 영향

우나리야,이민선,박수진,강명화 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5

Different types of coagulant and characteristics of its concentration added in the process of making milk curd were compared in this study. The pH of whey decreased when the amount of coagulant increased. The turbidity of whey was decreased when 5% of acetic acid and lemon juice were put into as coagulants. The texture of milk curd made with cow's milk and skim milk was measured the highest when acetic acid and lemon juice were added at 3, 5% of their concentration. The fracturability of milk curd made only with cow's milk decreased as the concentration of each coagulant increased. The cohesiveness was decreased as concentration of coagulant increased. The springiness was slightly changed depending on its coagulant but didn't show much of difference. The gummminess of milk curd made with cow's milk was increased when 3, 5% of coagulant was added. The result of sensory evaluation of milk curd showed that preference of milk curd wasn't depending on types of milk nor its coagulant. Also, flavor preference showed better when lemon juice was added. Preference in texture of milk curd was the highest made with cow's milk and skim milk. Preference in taste was high when 10% of lemon juice was added to skim milk and preference in its appearance showed higher when the alum was added as a coagulant to both cow's milk and skim milk than other coagulants.

Pseudomonas fluorescens의 Salicylate hydroxylase를 암호하는 유전자의 클로닝

정유선,민경희,이나리 숙명여자대학교 자연과학연구소 1997 자연과학논문집 Vol.- No.8

Pseudomonas fluorescens SME11로 부터 salicylate hydroxylase를 암호하는 nahG 유전자를 클로닝하기 위하여 NAH플라스미드를 주형으로 하여 PCR을 수행하였다. PCR방법으로 얻은 1.6kb DNA절편을 pT7Blue(R)벡터에 삽입시켜 재조합 플라스미드 pNY1을 제조하였으며 이것을 E. coli에 형질전환하였다. 재조합 DNA를 제한효소로 처리하여 EcoRⅤ, KpnⅠ, PvuⅡ, StuⅠ등이 존재함을 확인하였으며, 이것으로 간단한 제한효소지도를 작성하였고 재조합 DNA를 한 방향으로 deletion하여, nahG유전자를 포함한 DNA절편의 염기서열을 결정하였다. 재조합 플라스미드인 pNY1에는 1,305bp크기의 open reading frame이 존재하였으며, salicylate hydroxylase에 해당하는 434개의 아미노산을 암호하였다. 아미노산서열을 비교한 결과, P.putida KF715와 84.1%, P.putida PpG7과 71.4%, P.putida S-1 과 48.7%의 homology를 보였으며, 또한 Sphingmonas sp.와 26.8%의 homology를 보였다. Pseudomonas fluorescens SME11 carrying NAH plasmid was isolated from wastewater. To clone nahG gene encoding salicylate hydroxylase, 1.6kb DNA fragment of PCR product from NAH plasmid was inserted to pT7Blue(R) vertor and resulting recombinant DNA was named pNY1. The transformants, E.coli SMY1, clone containing the recombinant plasmid pNY1 were able to convert salicylate to catechol and produced dark brown color caused by accumulation and auto-oxidation of catechol. Restriction endonuclease mapping of 1.6kb size of the insert of the recombinant plasmid pNY1 was carried out with EcoRⅤ, KpnⅠ, PvuⅡ, StuⅠ. By means of unidirectional ExoⅢ deletion and dideoxynucleotide chain termination, we determined the nucleotide sequence of the DNA fragment containing nahG gene. One open reading frame of 1,305 bp corresponding to 434 amino acids was found in the insert DNA. Deduced amino acid sequences of the nahG gene showed 84.1%, 71.4%, 48.7%, and 26.8% homologies to those from P.putida KF715, P. putida PpG7, P. putida S-1, and Sphingmonas sp., respectively.

Characterization of biphenyl biodegradation, and regulation of biphenyl catabolism in Alcaligenes xylosoxydans

Lee, Na-Ri,On, Hwa-Young,Min, Kyung-Hee 숙명여자대학교 환경과학연구센터 1996 환경과학논문집 Vol.4 No.-

A strain of Alcaligenes xylosoxydans SMN3 capable of utilizing biphenyl was isolated from the sewage. This strain grew not only on biphenyl, phenol, and benzoate, but also on salicylate. The ca-tabolism of biphenyl and salicylate is interrelated, since benzoate is common metabolic in­termediates of biphenyl and salicylate. Enzyme levels in the extracts of benzoate­grown stain SMN3 cells clearly showed that benzoate induced catechol 2,3­oxygenase which in meta­cleavage enzyme of catechol, not induced catechol 1,2­oxygenase. All the oxidative enzymes of biphenly and 2,3­dihydroxybiphenyl (23DHBP) degradative pathways were induced when the cells were grown on bi­phenly or saliclyate, respectively. Biphenyl can be common inducer in the oxidation of biphenyl and 2,3­dihydroxybiphenyl, in addition salicylate was also good inducer for the 2,3­dihydroxybiphenyl dioxygenase. The two metabolic pathways for the degradation of biphenyl and salicylate are induced after growth on either biphenyl of salicylate, suggesting a common regulatory element. However, benzoate cannot induce the enzymes responsible for the oxidation of these compounds. It was clear­ly shown that biphenyl and salicylate were good inducers for indigo formation due to the activity of biphenyl dioxygenase. The strains of several bacteria that oxidize hydrocarbons including biphenyl to cis­dihydrodiols also oxidize indole to indigo. Our results suggest that indigo formation is due to the combined activities of tryptophanase and biphenyl dioxygenase.

New Records of Host Plant for Radopholus similis Isolated from Agathis dammara

Na-Ri Lee,Dong Woo Kim,Bo-ram Kim,Bok-ri Park,Jae yong Chun 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The aim of the study is to report Radopholus similis detected from Agathis dammara in Thailand as new host plant. Existing host of R. similis was known as coffee, pepper, sugarcane and banana etc. This nematode in this study was observed morphologic character using Carl Zeiss Axioimager M2 and Axiovision Rel. 4.8 program. The result shows that a and b of the female was distorted toward maximum value of original description of species, and b′, c and stylet length was distorted toward minimum value of original description of species. This nematode was morphologically distinguished from originally detected R. similis, but measured value was similar in range of original description of species. For more information, molecular assay was also confirmed the R. similis with 98% homology with the sequence of the internal transcribed spacer region (ITS) of ribosomal DNA. We provided PCR-amplified ITS nucleotide sequence.

Purification and Characterization of 2,3-Dihydroxybiphenyl 1,2-Dioxygenase from Comamonas sp. SMN4

( Lee Na Ri ),( Jang Mi Lee ),( Kyung Hee Min ),( Dae Young Kwon ) 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.4

Bacillus subtilis D7에 의하여 발효된 백태 청국장의 특성

Na-Ri Lee,황대연,손홍주,김동섭,박성보,이상미,정성윤,고태훈 한국생명과학회 2013 생명과학회지 Vol.24 No.4

This study was carried out to investigate the characteristics of white soybean Chungkookjang fermented by Bacillus subtilis D7. The highest germination rate was obtained under 25°C when water was supplied for 4 days at intervals of 2 hr. The total isoflavone content was 971.3 μg/g before germination and 1023.8 μg/g after germination. The amino type- and ammonia type-nitrogen contents of Chungkookjang were proportional to the fermentation time. The pH values of all Chungkookjang soybeans increased up to pH 7.8-8.0 during fermentation. The number of viable cells in all Chungkookjang soybeans increased significantly up to 24 hr. The protease activity of all Chungkookjang soybeans increased up to 30-36 hr. The α- and β-amylase activities of Chungkookjang fermented by B. subtilis D7 were enhanced with increasing fermentation time. Analysis of the effect of Chungkookjang intake on the liver function of mouse showed that the alkaline phosphatase (ALP) activity and the superoxide dismutase (SOD) activity in the Chungkookjang diet group were markedly higher than those in the control group. The asparatate aminotransferase (AST) activity in the germinated soybean Chungkookjang diet group was higher than that in the nongerminated soybean Chungkookjang diet group. Therefore, Chungkookjang soybeans fermented with B. subtilis D7 can be expected to have an increased content of functional components and improved quality characteristics. 본 연구에서는 Bacillus subtilis D7과 발아콩을 이용하여 발효된 청국장의 특성을 조사하였다. 백태는 6시간 침지 후, 25℃에서 2시간마다 4일 동안 물을 공급했을 때, 발아율이 가장 높았다. 총 이소플라본 함량은 발아 전 971.3 μg/g이었고, 발아 후 1023.8 μg/g이었다. 실험에 사용된 모든 청국장에서 아미노태 질소 및 암모니아태 질소 함량은 배양시간이 경과함에 따라 증가하였고, 청국장의 pH는 발효과정을 통하여 pH 7.8-8.0로 증가하였다. 청국장의 생균수는 발효 24시간까지 급증한 후, 약간 감소하였다. 청국장의 protease 활성은 발효 30-36시간까지 증가하였고, B. subtilis D7를 이용한 청국장의 α- 및 β-amylase 활성은 발효 12-18시간까지 증가하였다. 청국장을 섭취한 마우스의 ALP 활성과 SOD 활성은 대조군보다 매우 높았다. AST 활성은 미발아콩 청국장 섭취군보다 발아콩 청국장 섭취군에서 더 높았다. 결론적으로 B. subtilis D7을 이용하여 조제된 발아콩 청국장은 품질 특성이 향상되었고, 기능성 성분의 함량이 증가되었다.

Antimicrobial Activity of Myagropsis yendoi Ethanol Extract

Na bi PARK,So-Young LEE,So-Young YOON,Eu-Jin SONG,Koth-Bong-Woo-Ri KIM,So-Jeong LEE,Chung-Jo LEE,Ji-Yeon JUNG,Ji-Hee KWAK,Ki-Wan NAM,Dong-Hyun AHN 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

Paenibacillus oceanisediminis sp. nov. isolated from marine sediment.

Lee, Jina,Shin, Na-Ri,Jung, Mi-Ja,Roh, Seong Woon,Kim, Min-Soo,Lee, Jung-Sook,Lee, Keun Chul,Kim, Young-Ok,Bae, Jin-Woo Society for General Microbiology 2013 International journal of systematic and evolutiona Vol.63 No.2

<P>A Gram-stain-negative, non-motile, aerobic, endospore forming and rod-shaped bacterium, designated strain L10(T), was isolated from marine sediment collected from the South Korean coast. The organism grew optimally under conditions of 30 C, 1?% (w/v) NaCl and pH 6.0. It was oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L10(T) was associated with the genus Paenibacillus and most closely related to Paenibacillus barcinonensis BP-23(T) (98.2?% similarity). The major fatty acids of strain L10(T) were iso-C(14?:?0), anteiso-C(15?:?0) and iso-C(16?:?0). The cell-wall peptidoglycan was the A1γ type, and the predominant isoprenoid quinone was menaquinone-7. Strain L10(T) contained two unidentified lipids, an unidentified amino-phospholipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The G+C content of the genomic DNA was 44 mol% and the DNA-DNA hybridization values with closely related strains were below 142?%. Based on phenotypic, genotypic, and phylogenetic data, strain L10(T) should be classified as a novel species within the genus Paenibacillus. The name Paenibacillus oceanisediminis sp. nov. is proposed. The type strain is L10(T) (?=?KACC 16203(T)?=?JCM 17814(T)).</P>

GO-44 : Prognostic value of pre-treatment Hb level in patients with early cervical cancer

( Na Ri Shin ),( Yoo Young Lee ),( Kyoung A Lee ),( Seung Hyun Kim ),( Ji Young Kim ),( Chel Hun Choi ),( Tae Joong Kim,),( Jeong Won Lee ),( Duk Soo Bae ),( Byoung Gie Kim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

The purpose of this study is to investigate the prognostic role of pre-treatment anemia in patients with early cervical cancer who underwent radical hysterectomy In this study, we retrospectively enrolled patients with early cervical cancer (FIGO stage IB to IIA) who were treated at Samsung Medical Center, Seoul, Korea, from 1996 to 2007. We retrospectively enrolled 805 patients. Median pre-treatment Hb level was 12.8 g/dL (4.0-16.9) in all patients. 99 out of 805 patients had pre-treatment anemia (12.3%). Pre-treatment anemia was significantly associated with large tumor size, advanced clinical stage, and parametrial invasion. In multivariate analysis, higher pre-treatment Hb entailed better prognostic significance in disease free survival (HR, 0.88; 95% CI, 0.078-0.99) but not in overall survival (HR, 0.94; 95% CI, 0.80-1.10). In conclusion, we found that the negative association between pre-treatment Hb level and tumor size and the impact of anemia before treatment on disease free survival adjusted for other factors including clinical stage and pathological findings in early stage cervical cancer.

The Study on Miniaturization and Weight Reduction of Auxiliary Power Unit in Magnetic Levitation Train

Lee, Na Ri,Shin, Hee Keun,Choi, Sung Ho,Kim, Ju Bum,Lim, Jae Won,Park, Doh Young,Mok, Hyung Soo The Korean Society for Railway 2015 International Journal of Railway Vol.8 No.1

Due to the characteristics of the vehicle structure, the magnetic levitation train has a confined bottom space thus a study on miniaturization and weight reduction of auxiliary power unit is essential. This auxiliary power unit is an essential device used for illumination, air conditioning, heating and air brake equipment excluding the motor. The previous auxiliary power unit for magnetic levitation train has used the hard switching having a high switching frequency with heavy loss in order to reduce the size of filter reactor and transformer but the reduction in volume was not significant. In this paper, by reducing the loss, reducing the size of the cooling unit and by increasing the switching frequency using the soft switching of resonant converter, it has miniaturized and reduced the weight of filter reactor and transformer which occupy significant space in the auxiliary power unit. This study has verified the performance of 50KVA grade prototype through simulated interpretation and analysis, and compared the size and weight of auxiliary power unit of the previous magnetic levitation train.