간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

대학교 신입생의 특성에 관한 실태조사(Ⅱ) : 1995학년도 조선대학교 신입생을 대상으로 1995 chosun University Entrants

조용래,박상학,김상훈,표경식,김학렬,김양옥,김기순,박종 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

경피적 겸자 정복 및 골수강내 금속핀 고정을 이용한 쇄골 골절의 치료

홍기도,하성식,정남식,심재천,김경호 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적 : 경피적 겸자 정복 및 골수강내 금속판 고정술을 이용한 쇄골 간부 골절의 치료에 대해 유용성을 규명해 보고자 하였다. 대상 및 방법 : 2002년 1월부터 2003년 7월까지 본원에서 쇄골 간부 골절 환자 중 신경 및 혈관 손상이 없었고, 골절의 분쇄상이 비교적 적었던 총 16례를 대상으로 겸자를 이용한 경피적 정복 후에 골수강에 금속판을 삽입하여 고정하였다. 술 후 유합 정도 및 결과를 임상적, 방사선학적으로 분석하였다. 결과 : 방사선학적으로 골유합을 보인 경우가 총 15례였으며, 술 후 평균 9.1주에 유합 소견을 관찰하였다, 임상적으로 강 등의 기준에 따라 구분한 결과, 우수 이상이 14례로 나타났다. 1례에서 1주 만에 K-강선의 내측전위로 골절부위가 재전위되어 관혈적 정복 및 금속판 내 고정을 시행한 이외에 다른 주요 합병증은 없었다. 결론 : 저자들의 방법은 수술시간이 짧고 간단하며, 연부조직의 추가적 손상이 적어 골유합율이 높고, 수술 반흔을 남기지 않아 수술적 적응을 신중히 고려한다면 쇄골 간부 골절의 치료법의 하나로 유용할 것으로 사료된다. Purpose: To investigate the utility of surgical treatment of clavicle shan fracture using a percutaneous towel clip reduction and intramedullary fixation. Materials and Methods : This study was conducted for total 16 case of patients who had no neurovascular injury and a few comminuted bone fragment among patients with clavicle shaft fracture from January 2002 to July 2003. The method of operation was percutaneous towel clip reduction and intramedullary fixation. The clinical and radiological results were evaluated. Results : Radiologically, 15 cases showed bone unions and the avenge time was 9.1 weeks. According to Kang's criteria clinically, there were 14 cases which were more than an excellence. One case substituted open reduction and nailing fixation due to a medial migration of K-wire and re-displacement of fracture even in 1 week. However, there wasn't any other major complication. Conclusion : Due to its having no additional injury to soft tissues, no scar formations, and its short operation time, percutaneous towel clip reduction and intramedullary fixation will be very useful as one of the treatments of clavicular shaft fracture if it follows correct surgical indications.

Mitogenome descriptions of three new beetles belonging to Scarabaeiformia (Insecta: Coleoptera): Genomic comparison and phylogenetic analysis in Coleoptera

Min Jee Kim,Man Il Kim,Ki Gyoung Kim,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

Up to now only 17 coleopteran mitogenomes have been fully or near fully sequenced, but this chart does not include any complete mitogenome sequence of the coleopteran infraorder Scarabaeiformia, in which the monotypic Scarabaeoidea is included. In this study, we firstly present three scarabaeoid mitogenomes, two of which are completed and one is near completed. The complete nucleotide sequences of the white-spotted flower chafer, Protaetia brevitarsis (Scarabaeiformia: Scarabaeidae), was determined to be 20,319 bp, the two-spotted stag beetle, Metopodontus blanchardi (Scarabaeiformia: Lucanidae), was 21,678 bp, and the incomplete garden chafer, Polyphylla laticollis (Scarabaeiformia: Scarabaeidae), which lacks sequence information from whole A+T-rich region and a partial srRNA gene was 14,473 bp. Thus, the two complete mitogenome sequences are longest among the completely sequenced insect mitogenomes, followed by the 19,517 bp-long dipteran Drosophila melanogaster. Phylogenetic analysis using the concatenated 13 amino acid sequences of PCGs of the 13 coleopteran insects including three newly sequenced scarabaeoid successfully recovered a monophyly of suborder Polyphaga, a monophyly of infraorder Cucujiformia, a monophyly of infraorder Elateriformia, and also a monophyly of infraorder Scarabaeiformia within Polyphga. However, the Scarabaeiformia represented by three newly sequenced species was unexpectedly placed as a sister group to the Cucujiformia, instead of the placement to the sister to the Cucujiformia and Elateriformia group.

Complete mitochondrial genome sequence of the yellow-spotted long-horned beetle Psacothea hilaris (Coleoptera: Cerambycidae) and phylogenetic analysis among coleopteran insects

Kim, Ki-Gyoung,Hong, Mee Yeon,Kim, Min Jee,Im, Hyun Hwak,Kim, Man Il,Bae, Chang Hwan,Seo, Sook Jae,Lee, Sang Hyun,Kim, Iksoo Springer-Verlag 2009 Molecules and cells Vol.27 No.4

<P>We have determined the complete mitochondrial genome of the yellow-spotted long horned beetle, Psacothea hilaris (Coleoptera: Cerambycidae), an endangered insect species in Korea. The 15,856-bp long P. hilaris mitogenome harbors gene content typical of the animal mitogenome and a gene arrangement identical to the most common type found in insect mitogenomes. As with all other sequenced coleopteran species, the 5-bp long TAGTA motif was also detected in the intergenic space sequence located between tRNA(Ser)(UCN) and ND1 of P. hilaris. The 1,190-bp long non-coding A+T-rich region harbors an unusual series of seven identical repeat sequences of 57-bp in length and several stretches of sequences with the potential to form stem-and-loop structures. Furthermore, it contains one tRNA(Arg)-like sequence and one tRNA(Lys)-like sequence. Phylogenetic analysis among available coleopteran mitogenomes using the concatenated amino acid sequences of PCGs appear to support the sister group relationship of the suborder Polyphaga to all remaining suborders, including Adephaga, Myxophaga, and Archostemata. Among the two available infraorders in Polyphaga, a monophyletic Cucujiformia was confirmed, with the placement of Cleroidea as the basal lineage for Cucujiformia. On the other hand, the infraorder Elateriformia was not identified as monophyletic, thereby indicating that Scirtoidea and Buprestoidea are the basal lineages for Cucujiformia and the remaining Elateriformia.</P>

Complete Mitochondrial Genome Sequence of the Yellow-spotted Long-horned Beetle Psacothea hilaris (Coleoptera: Cerambycidae) and Phylogenetic Analysis among Coleopteran Insects

Ki Gyoung Kim,Mee Yeon Hong,Min Jee Kim,Man Il Kim,Chang Hwan Bae,Sook Jae Seo,Sang Hyun Lee,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

We have determined the complete mitochondrial genome of the yellow-spotted long horned beetle, Psacothea hilaris (Coleoptera: Cerambycidae), an endangered insect species in Korea. The 15,856-bp long P. hilaris mitogenome harbors gene content typical of the animal mitogenome and a gene arrangement identical to the most common type found in insect mitogenomes. As with all other sequenced coleopteran species, the 5-bp long TAGTA motif was also detected in the intergenic space sequence located between tRNASer (UCN) and ND1 of P. hilaris. The 1,190-bp long non-coding A+T-rich region harbors an unusual series of seven identical repeat sequences of 57-bp in length and several stretches of sequences with the potential to form stem-and-loop structures. Furthermore, it contains one tRNAArg-like sequence and one tRNALys-likes equence. Phylogenetic analysis among available coleopteran mitogenomes using the concatenated amino acid sequences of PCGs appear to support the sister group relationship of the suborder Polyphaga to all remaining suborders, including Adephaga, Myxophaga, and Archostemata. Among the two available infraorders in Polyphaga, a monophyletic Cucujiformia was confirmed, with the placement of Cleroidea as the basal lineage for Cucujiformia. On the other hand, the infraorder Elateriformia was not identified as monophyletic, thereby indicating that Scirtoidea and Buprestoidea are the basal lineages for Cucujiformia and the remaining Elateriformia.

Geographic Genetic Contour of the Dung Beetle, Copris tripartitus (Coleoptera: Scarabaeidae), that Is Endangered in Korea

Ki-Gyoung Kim,Ah Rang Kang,Min Jee Kim,Iksoo Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

The dung beetle, Copris tripartitus, (Coleoptera: Scarabaeidae), is one of the insect species listed as endangered wildlife in Korea. In order to establish conservation strategy an investigation on nation-wide genetic magnitude and nature of genetic diversity would be required. In this study, we sequenced each partial sequences of mitochondrial COI (658 bp) and CytB (433 bop) genes and complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA (411~420 bp) from 70 individuals of C. tripartitus collected from five Korean localities. The sequence divergence of mitochondrial genes was unexpectedly substantial as 5.0% (33 bp) for COI and 4.6% (20 bp) for CytB, whereas that of ITS2 was only 1.9% (6 bp), revealing much higher variability in mitochondrial DNA. In phylogenetic analysis, each 57 and 47 haplotypes obtained from COI and CytB gene sequences was subdivided into two groups (groups A and B), but the node supports for each group was not strong enough to consider each group as independent monophyletic groups in both genes. This interpretation was further supported by the same analysis with ITS2, showing no subdivision at all. These findings reinforce importance for the inference of phylogenetic result using both mitochondrial and nuclear encoded sequences. The C. tripartitus occurring in Korean peninsula was genetically well connected to each other and no obvious population genetic structure was detectable.

Description of Nearly Completed Mitochondrial Genome Sequences of the Garden Chafer Polyphylla laticollis manchurica, Endangered in Korea (Insecta: Coleoptera)

Min Jee Kim,Ki-Gyoung Kim,Iksoo Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

We present the nearly complete mitogenome sequences of the garden chafer, Polyphylla laticollis manchurica, which is listed as an endangered species in Korea. The P. l. manchurica mitogenome, which includes unfinished whole A+T-rich region and a partial srRNA was 14,473-bp long, possessing typical sets of genes (13 PCGs, 22 tRNA genes, and 2 rRNA genes). Gene arrangement of the P. l. manchurica mitogenome was identical to the common one found in the majority of insects. The 5 bp-long motif sequence (TAGTA) that has been suggested to be the possible binding site for the transcription termination peptide for the major-strand was also found in the P. l. manchurica mitogenome between tRNASer(UCN) and ND1. As has been previously determined, the high A/T content was unanimously observed in P. l. manchurica in terms of A/T bias in the third codon position (73.5%) compared with the first (66.4%) and second codon position (66.2%), and a high frequency of A/T-containing codons (a total of 28.22% for TTA, ATT, TTT, and ATA). The PCGs encoded in major-strands are slightly T-skewed, whereas those of the minor-strand are A-skewed, indicating strand asymmetry in nucleotide composition in the Coleoptera including P. l. manchurica.

Complete Nucleotide Sequence and Organization of the Mitogenome of the Endangered Eumenis autonoe (Lepidoptera: Nymphalidae) and Phylogenetic Analysis among Lepidopteran Insects

Min Jee Kim,Man Il Kim,Ah Rang Kang,Ki Gyoung Kim,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Eumenis autonoe belonging to a lepidopteran family Nymphalidae (superfamily Papilionoidea) is an endangered species in Korea. Historically, the species was distributed in Europe and Asian region including a wide region in Korean peninsula. However, in Korean peninsula, the species is found only in two isolate dregions: South in a remote island Jeju, where altitude is higher than1, 400 meter on Halla Mt. and North in far northern Korean peninsula around Mt. Bekdu. In this study, we report the complete mitochondrial genome of the endangered E. autonoe collected from Mt. Halla. The 15,489-bp long E. autonoe genome has a typical gene content found in animal mitochondrial genomes and contains the gene arrangement identical to all other sequenced lepidopteran insects, which differs from the most common type found in insects, as the result of the movement of tRNAMet to a position 5’-upstream of tRNAIle. As seen in many other lepidopteran insects, no typical ATN codon for COI gene is available. Thus, we tentatively designated the CGA (arginine) found at the beginning of the COI gene, as has been suggested for lepidopteran COI starter. The intergenic spacer sequence located between tRNASer (UCN) and ND1 of E. autonoe mitogenome also contains the ATACTAA motif which is conserved in all sequenced lepidopteran species. The 678-bp long A+T-rich region, which is longest in sequenced lepidopteran insects contains ten identical tandem repeats composed of 27 bp plus one 13-bp long identical incomplete final repeat. Such repeat sequence is rare in the lepidopteran mitogenomes known so far. The E. autonoe A+T-rich region also contains a poly-T stretch located at the end of the region as 19 bp and also contains the downstream conserved motif ATAGA that were previously suggested to serve as a structural signal for minor-strand mtDNA replication. Phylogenetic analysis using the concatenated 13 amino acid sequences of PCGs among available six lepidopteran superfamilies (Tortricoidea, Pyraloidea, Papilionoidea, Bombycoidea, Geometroidea, and Noctuoidea) rooted with three dipteran species with BI and ML analyses supported the following topology: ((((Bombycoidea + Geometroidea +Noctuoidea) + Papilionoidea) + Pyraloidea) + Tortricoidea). Within Papilionoidea, a closer relationship between Lycaenidae and Pieridae, excluding Nymphalidae was observed. Further fruitful information will be available after more analysis is done.

Mitochondrial DNA Sequence Variation of the Tiny Dragonfly, Nannophya pygmaea (Odonata: Libellulidae)

Ki Gyoung Kim,Sang Kyun Jang,Dong Woo Park,Mee Yeon Hong,Kyoung Hee Oh,Kee Young Kim,Jae Sam Hwang,Yeon Soo Han,Ik Soo Kim 한국잠사학회 2007 International Journal of Industrial Entomology Vol.15 No.1