정신분열병 환자의 인터류킨-2 생산능

이선미,박주홍,김동인,은헌정,김임,김경용 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.4

급성기 및 관해상태의 정신분열병 환자와 정상 대조군의 IL-2 생산능을 비교함으로써, 정신분열병의 여러 발병원인 중 자가면역 병인론의 증거인 세포성 면역방응의 저하 및 질병상태와 관련한 세포성 면역기능의 변화를 일부 한국인 정신분열병 환자에서 조사에서 조사하고자 하였다. 방 법 : DSM-IV의 정신분열병 진단기준에 적합한 급성기 환자 19명(남 : 10, 여 : 9)과 관해상태의 환자 21명(남 :12, 여: 9) 및 정상대조군 40명(남 : 20, 여 : 20)을 연구대상으로 하였다. 피험자로부터 채혈한 혈액에서 임파구를 분리하여 phytohemagglutinin으로 T임파구를 자극하고 세포배양하여 증식시킨후 IL-2immunoassay kit을 이용하여 T임파구의 IL-2 생산능을 측정하였다. 결 과 : IL-2 생산능은 성별에 관계없이 정신분열병 환자군에서 정상 대조군에 비하여 유의하게 감소하였다. (t=5. 97, p<0.001). 질병상태에 따라 급성기 정신분열병 환자군 및 관해상태 정신분열병 환자군으로 구분하여 정상대조군의 IL-2 생산능과 비교한 결과, IL-2 생산능이 정상 대조군에서 가장 높았고 다음은 관해상태 환자군이었으며 급성기 환자군에서 가장 낮았다. 세 군 사이에는 모두 유의한 차이가 있었다. (F=35.35, p<0.001). 결 론: 일부 한국인 정신분열병 환자에서 질병상태와 유의하게 관련된 세포성 면역기능의 저하가 관찰되며, 이는 정신분열병의 다양한 원인중 자가면역 기전에 의한 발병을 시사한다. Objects : This study was designed to examine the decreased cellular immune response and the change in cellular immunity related to to clinical status in Korean schizophrenic patients. Methods : The subjects were 19 acute schizophrenics and 21 remitted schizophrenics who met the DSM-IV criteria of schizophrenia and 40 healthy volunteers as the normal control. After the lymphocyte was separated from blood which had been drawn from the subjects and then T lymphocyte was stimulated by PHA and proliferated by cell culture, the examiner measured IL-2 productivity by IL-2 immunoassay kit. Results : IL-2 productivity of the schizophrenics was significantly lower than that of the normal controls. There was no significant difference between male and female in two groups. In the comparison by clinical status, acute schizophrenic group was the lowest, remitted schizophrenic group was next, and normal control group was the highest in the IL-2 productivity, with statistically significant difference among the groups(p<0.001). Conclusion : We suggest that there is a decrease in the cellular immune response which is significantly related to clinical status in some Korean schizophrenics. This finding supports that onset of schizophrenia may be the result of autoimmune mechanism, which is one of the various etiologic factors in schizophrenia.

어린이의 하악과두골절에 대한 비외과적 정복 및 악기능회복의 치험례

이애련,김신,김주미,황보민,김동규,최외임 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

The author treated a case of mandibular left condylar fracture in children with conservative measures utilizing elastic rubbers, and obtained the following results. 1. It will be better to adapt non-surgical conservative method based upon functional orthopedic approaches in condylar fractures of growing children, with the exception in the cases with bilateral fracture concomittent with displacement. 2. The treatment should be initiated soon after infliction of trauma and take more than 6 months of treatment-period. 3. In order to prevent the articular ankylosis, it would be better to minimize the period of intermaxillary fixation and allow the functional mandibular movement as soon as possible. In this case, a little more treatment for orthodontics and follow-up observation would be required.

유전치부의 심미수복을 위한 Silicoated Resin-bonded stainless steel crown의 제작 및 치험례

이애련,김신,최외임,황보민,김주미,김동규 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

For the purpose of establishing the method of esthetic full coverage restoration with superior esthetics and strength for primary anterior teeth, a trial was made with silicoated resin-bonded stainless steel crown with enhanced bond strength between stainless steel and composite resin. The results were as follows: 1. The result was revealed superior in the aspects of esthetics, strength, color stability, labial thickness and chairtime which is very significant especially for un-cooperative children. 2. On the contrary, the necessity of more than 2 appointments and the aspect of economy due to the lack of common-use were remained to be solved from now on.

잉어류 바이러스성전신괴사증바이러스 (VSNCV) 백신 투여에 대한 잉어의 면역반응

조미영,손상규,김이청,김진우,오명주,정성주,박수일 한국어병학회 2003 한국어병학회지 Vol.16 No.3

양식 잉어류에서 심각한 피해를 야기하고 있는 VSNCV에 대한 백신을 제작하며 잉어에 투여한 후 특이적 및 비특이적 면역반응을 조사하였다. 백신은 포르마린처리백신 (FKV)과 가온처리백신 (HKV)를 제작하여 건강한 잉어에 0.2㎖씩 복강주사 하였으며, 2주 후 동일한 방법으로 boost처리하였다. 백신 투여 후 비특이적 면역반응 중 혈청 라이소자인 활성과 대식세포의 chemiluminescent (CL) reponses은 1차 주사 후 및 boost 처리 후 2일째부터 7일째까지 유의적인 증가를 나타내었으며, 이후 대조구 수준으로 감소하였다. ELISA법으로 항체가를 조사한 결과 대조구를 제외한 FKV와 HKV 투여구에서 주사 후 2주 째부터 boost처리 후 6주 째까지 유의적으로 증가하였으며, 그 중에서도 boost 처리 후 2주 째에 VSNCV로 공격 실험한 결과 FKV 투여구에서는 20%의 누적폐사율을 나타내어 방어력이 인정 되었으나, HKV투여구에서는 70%의 누적폐사율이 나타나 방어효과가 거의 없는 것으로 나타났다. VSNC is a viral disease causing significant economic losses in cultured carp Ciprinus carpio L. in Korea. Carps were immunized with prepared vaccines against VSNCV and examined specific and nonspecific immune responses. Carps were injected by 0.2㎖ of formalin-killed vaccine(FKV), heat-killed vaccine(HKV) or E-MEM, respectively and dealt with boost with same way two weeks later. The lysozyme activity of serum and chemiluminescent reponses of head-kidney leucocytes showed increased responses during 2-7 days post-first injection(pfi) and post-boost(pb) in the vaccinated fish, and then decreased to the level of control. As measured by ELISA, vaccinated groups showed a significant increase in VSNCV-specific serum antibodies between 2 weeks pfi and 6 weeks pb with a peak at 2 weeks pb. Results of the virus challenge showed that the fish vaccinated with FKV have induced protective immunity, white HKV injection hardly provided protection.

여대생의 결혼관에 관한 연구

강신미,김욱련,김이화,김현주,백은주 효성여자대학교 가정대학 학도호국단 1985 家政大論集 Vol.4 No.-

In these days, Korea has undergone various changes in every social aspect throughthe process of modernization. Especially in instituional and actual aspects, the family.and marriage have been changed greatly by the rapid urbanization and iudustrializatton,.and these changes have been investigated scientifically. This paper presents marital consciousness and actual behavior of 300 woman collegestudents in Daegu, and was surveyed comparing various parts-the humanities, natural·science and the arts. As for the basic attitude of marriage, they were positive in the need of marriage,,defined marriage as the second starting point of life. Also they put the aim of marriage,on mental value. As for the dating and purity before marriage, 84 percent of respondents assented tothe dating before marriage. And they thought it proper that the duration of datingwas one year to two years. The age of the first dating and the standard of sex moralstore significant. The part of the arts tended to be open, and the part of natural sciencewere conservative. As for the standard of spouse's choice, 84 percent of respondents wanted to have theright of priority they could choose their spouses for themselves. And they thought it·proper that the age of marriage was 25-year-old to 26-year-old, and the gap of maritalage was one year to three years. Ideal spouse's characteristic, home surroundings, and marital harmony were significant. Among various marital conditions, personality tended to to emphasized, and theatmosphere of family and masculine appearance were taken serious. As for a. spouses's job, professor or research occupation was popular. Compared withthe part of the arts, the part of the humanities and natural science were positive inpredicting marital harmony. Among various marital conditions, an only son, the eldest son, and an offspring ofa concubine tended to be evaded. The type of an ideal spouse was companionship.

천연 생약재 열수 및 알코올 추출물의 어병 세균에 대한 항균력

최혜승,김이청,이주석,조미라,서창호,박수일 한국어병학회 2004 한국어병학회지 Vol.17 No.1

감초 외 48종의 천연 생약제로부터 열수 및 알콜 추출액을 제작하여 19 균주 어병 세균을 대상으로 항균력을 조사한 결과, 알콜 추출액은 22종, 열수 추출액은 16종이 어병 세균에 대하여 항균력을 나타내며 이 중 13종의 생약재는 알콜 및 열수 추출액 모두 8㎜ 이상의 저지대를 나타내어 항균력이 있는 것으로 나타났다. 열수 추출물에서 항균력을 나타내는 약재 중 그람 음성균에 감수성이 양호한 약재는 저지대 8 ㎜ 이상을 나타내는 애엽, 개옻나무, 연교, 지유, 파엽, 대황 및 황금이었으며, 그람 양성균에 항균력이 양호한 약재는 삼지구엽초, 육계 및 보골지 등 이었다. 그리고 오매, 황련, 계혈등, 상백피, 오배자 및 오미자는 저지대가 8㎜ 이상으로 그람 음성,양성균 모두에 항균력이 있었다. 알콜 추출물중에서 그람 음성균에 항균력을 나타내는 것은 백작약, 오매, 선모 및 황금 등으로 저지대가 8㎜ 이상으로 측정되었으며, 그람 양성균에 항균력을 나타내는 것은 감초, 계혈등, 단삼, 상백피, 육계 및 보골지 등으로 저지대가 8㎜ 이상이었다. 또한 그람 음성, 양성균에 모두 8㎜ 이상의 저지대를 나타내는 것은 애엽, 개옻나무, 황련, 지유, 오배자 및 오미자 등이었다. 그러나 이 중에서 오배자 열수 추출물의 어병 세균에 대한 발육 저지대가 32㎜로 그람 음성, 양성균 모두에 가장 뛰어난 항균력을 나타내어 다른 생약재와 다른 광범위 생약재인 것으로 나타났다. 애엽, 황금, 지유, 오매, 황련 및 오배자 열수 ·알콜 추출물은 tetracycline에 내성을 나타내는 균주에 감수성을 나타내었다. Hundreds of medicinal herbs have been using for the purpose of diseases treatment and immune enhancement for human being and other animals including fishes. Among them, 49 species of medicinal herbs were selected and tested for antibacterial activities against 19 strains of fish pathogenic bacteria in different 4 species. The 49 medicinal herbs were extracted by water and ethyl alcohol. The extracts were freeze dried and some paper discs from the extracts were prepared for the evaluation of antibacterial activity. The tested pathogenic bacteria were 5 strains of Edwardsiellu tarda, 5 strains of Vibrio sp., 4 strains of Lactococcus gawieae, 1 strain of Lactococcus raffinose. 1 strain of Streptococcus parauberius, and 3 strains of Streptococcus iniue. The Galla rhois (Obaeja), Gaeonnamu and Hwangleyon showed antibacterial activities on both gram negative and gram positive fish pathogenic bacteria. The Youkgae, Sangbaekpi, Bogolji and Gamcho showed very effective antibacterial activities on gram positive pathogens while Jiyu, Aeyoeb and Yeonkyo showed very effective on gram negative pathogens.

정신분열병 환자의 인터류킨-2 생산능

김경용,김임,박주흥,은헌정,김동인,이선미 예수병원 1997 예수병원학술지 Vol.18 No.-

REVIEW : Epigenetic Alterations in Inflammatory Bowel Disease and Cancer

( Joo Mi Yi ),( Tae Oh Kim ) 대한장연구학회 2015 Intestinal Research Vol.13 No.2

Overwhelming evidences supports the idea that inflammatory bowel disease (IBD) is caused by a complex interplay between genetic alterations of multiple genes and an aberrant interaction with environmental factors. There is growing evidence that epigenetic factors can play a significant part in the pathogenesis of IBD. Significant effort has been invested in uncovering genetic and epigenetic factors, which may increase the risk of IBD, but progress has been slow, and few IBD-specific factors have been detected so far. It has been known for decades that DNA methylation is the most well studied epigenetic modification, and analysis of DNA methylation is leading to a new generation of cancer biomarkers. Therefore, in this review, we summarize the role of DNA methylation alteration in IBD pathogenesis, and discuss specific genes or genetic loci using recent molecular technology advances. Here, we suggest that DNA methylation should be studied in depth to understand the molecular pathways of IBD pathogenesis, and discuss epigenetic studies of IBD that may have a significant impact on the field of IBD research. (Intest Res 2015;13:112-121)

Biological significance of epigenetic alterations by radiation in human cancer

Joo Mi Yi 대한방사선방어학회 2019 대한방사선방어학회 학술발표회 논문요약집 Vol.2019 No.04

Identification and Phylogeny of the Human Endogenous Retrovirus HERV-W LTR Family in Cancer Cells

Yi, Joo-Mi,Kim, Hwan-Mook,Kim, Heui-Soo The Korean Society for Integrative Biology 2002 Korean journal of biological sciences Vol.6 No.2

The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV-W LTR elements in various cancer cells (2F7, A43l , A549, HepG2, MIA-PaCa-2, PC-3, RT4, SiHa, U-937, and UO-31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (88-99%) with HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV-W LTR elements (RT4-2, A43l-1, and UO3l-2) belonged to Group 1, whereas nine LTR elements (2F7-2, A549-1, A549-3, HepG2-3, MP2-2, PC3-1, SiHa-8, SiHa-10, and U937-1) belonged to Group 11. Taken together, our new sequence data of the HERV-W LTR elements may contribute to an understanding of tissue-specific cancer by genomic instability of LTR integration.