Correlation of CT Perfusion Images with VEGF Expression in Solitary Brain Metastases

Zhang, Jian-Hua,Wang, Ming-Sheng,Pan, Hai-Hong,Li, Shu-Feng,Wang, Zhong-Qiu,Chen, Wang-Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objectives: To obtain permeability surface (PS) values using multi-slice helical CT perfusion imaging and to evaluate the spatial distribution correlation between PS values and vascular endothelial growth factor (VEGF) expression in solitary brain metastases. Methods: Imaging was performed on 21 patients, PS values being calculated from the central, border and peripheral parts of tumours. VEGF expression was determined by immunohistochemical staining. Results: Rim enhancement was found in 16 cases, the border of the tumour featuring PS elevation with high VEGF expression in 13 cases. In the 5 cases with nodular enhancement, the border and the central part had high permeability and VEGF expression was high in all cases, the correlation being significant (P<0.01). Conclusion: VEGF expression in brain metastases positively correlates with PS values from CT perfusion imaging, so that the latter can be used in the surveillance of angiogenic activity in brain metastases.

Coenzyme Q10 Production by Sphingomonas sp. ZUTE03 with Novel Precursors Isolated from Tobacco Waste in a Two-Phase Conversion System

( Le Quan Qiu ),( Wei Jian Wang ),( Wei Hong Zhong ),( Li Zhong ),( Jian Jun Fang ),( Xuan Zhen Li ),( Shi Jin Wu ),( Jian Meng Chen ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.5

Coenzyme Q10 (CoQ10) is a widely used supplement in heart diseases treatment or antioxidative dietary. The microbial production of CoQ10 was enhanced by addition of solanesol and novel precursors recovered from waste tobacco. The novel precursors were separated by silica gel and identified as α-linolenic acid (LNA) and butylated hydroxytoluene (BHT) based on the effect on CoQ10 production and GC-MS. The effects of novel precursors on CoQ10 production by Sphingomonas sp. ZUTE03 were further evaluated in a two-phase conversion system. The precursor`s combination of solanesol (70mg/l) with BHT (30mg/l) showed the best effect on the improvement of CoQ10 yield. A maximal CoQ10 productivity (9.5 mg l-1 h-1) was achieved after 8h conversion, with a molar conversion rate of 92.6% and 92.4% on BHT and solanesol, respectively. The novel precursors, BHT and LNA in crude extracts from waste tobacco leaves, might become potential candidates for application in the industrial production of CoQ10 by microbes.

Impact of Education on Multidimensional Poverty Reduction at the Post-Poverty Alleviation Era in Xinjiang

Jian Qiu,Hongsen Wang,Ailida Aikerbayr 대외경제정책연구원 2023 East Asian Economic Review Vol.27 No.3

The multidimensional poverty index is an indicator system established for defining and evaluating poverty, to understand poverty in dimensions beyond just monetary scarcity. Based on income, education, health, living standards, and social dimensions, this article measures and analyzes the level of multidimensional poverty in Xinjiang using the Alkire-Foster method, with cross-sectional data obtained from a 2022 survey. Probit model is constructed for regression analysis, further considering the impact of education on enhancing feasible capabilities and alleviating multidimensional poverty at the post-poverty alleviation era. The data shows that many people still face significant challenges from the perspective of multidimensional poverty; the decomposition results of each dimension show that education contributes more to the multidimensional poverty; the regression analysis results show that the higher the education level, the lower the multidimensional poverty; heterogeneity analysis revealed that the inhibitory effect of education on multidimensional poverty is greater for females than males, and the poverty reduction effect of education mainly concentrates on middle-aged and older individuals. This article is meaningful for exploring strategies to alleviate multidimensional poverty in ethnic minority regions in frontier areas in the new era, accelerating regional economic development, and achieving shared prosperity.

Imaging Findings of Follicular Dendritic Cell Sarcoma: Report of Four Cases

Qiu Long-Hua,Xiao Qin,Gu Ya-Jia,Wang Jian,Feng Xiao-Yuan 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.1

Follicular dendritic cell sarcoma is a rare malignant neoplasm and little is known about its radiological features. We present here four cases of follicular dendritic cell sarcomas and we provide the image characteristics of these tumors to help radiologists recognize this entity when making a diagnosis.

An Increase in Synaptic NMDA Receptors in the Insular Cortex Contributes to Neuropathic Pain

Qiu, Shuang,Chen, Tao,Koga, Kohei,Guo, Yan-yan,Xu, Hui,Song, Qian,Wang, Jie-jie,Descalzi, Giannina,Kaang, Bong-Kiun,Luo, Jian-hong,Zhuo, Min,Zhao, Ming-gao AAAS 2013 Science signaling Vol.6 No.275

<P><B>Stopping the Pain</B></P><P>Damage to the central or peripheral nervous system can trigger the development of neuropathic pain, which can manifest as painful sensations in response to stimuli that are not normally painful. Qiu <I>et al.</I> found that mice that had developed neuropathic pain after peripheral nerve injury showed changes in synaptic plasticity and increased abundance of synaptic NMDA receptors in the insular cortex, a region of the brain that is activated by acute and chronic pain. Using pharmacological inhibitors and transgenic mice, they mimicked these changes in vitro with insular cortical slices and thus identified the signaling pathway responsible. Mice injected with NMDA receptor inhibitors showed reduced behavioral signs of neuropathic pain after peripheral nerve injury. Thus, blocking NMDA receptor function in the insular cortex may prevent the development of neuropathic pain.</P>

Ellipsometric Study of the Optical Properties of Silver Oxide Prepared by Reactive Magnetron Sputtering

Jian-Hong Qiu,Liang-Yao Chen,Jia-Ni Yu,Jing Li,Peng Zhou,Qing-Hai ong,ong-You Wang,Xiao-Yong Gao,Yue-Mei Yang,Yu-Xiang Zheng 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.2

A series of AgOx thin films were prepared by the DC-magnetron reactive-sputtering method at room temperature under different sputtering power and oxygen to argon ratio conditions. The optical properties of the AgOx films were studied by analysis of the spectroscopic ellipsometry data with the Tauc-Lorentz multi-oscillator model in the 1.5 4.5-eV photon-energy range. The spectra of absorption, XPS and PL were measured. The results indicate that the AgOx film changes from metallic to dielectric structure with increasing oxygen flux. Under the saturated oxidation condition after annealing, the dominating component will be Ag2O in the film to show indirect interband transitions occurring at about 0.57 eV and 2.43 eV, respectively, that are in agreement with the results of PL measurement and Lorentz analysis.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Screening and Characterization of an Aerobic Nitrifying-denitrifying Bacterium from Activated Sludge

Xiaofan Qiu,Tianwen Wang,Xiaomin Zhong,Guocheng Du,Jian Chen 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2

Taking advantage of the good biocompatibility and high efficiency of nitrogen removal with microbes,nitrifying and denitrifying bacteria, are becoming increasingly more widely used for wastewater treatment and prevention of eutrophication. In this research, an aerobic nitrifying-denitrifying bacterium was successfully screened from activated sludge and identified as Pseudomonas sp. (CCTCC No M2010209) by the 16S rDNA sequence. The activity verification confirmed its nitrifying-denitrifying capability of removing ammonium, nitrate and nitrite nitrogen. The types of carbon sources and carbon-nitrogen ratio greatly influenced the removal efficiency of NH4+-N and NO3−-N. When the initial concentrations of NH4+-N and NO3−-N in synthetic wastewater were less than 70 and 50mg/L, the nitrogen removal rates reached 94 and 90% in 9 h, respectively. Preliminary comparisons of nitrogen removal capacity between this isolate and other commercial preparations in the treatment of synthetic wastewater revealed its promising potential to be used in the actual wastewater treatment.

Experimental study on the Influence of Heating Surface Inclination Angle on Heat Transfer and CHF performance for Pool Boiling

Chenglong Wang,Panxiao Li,Dalin Zhang,Wenxi Tian,Suizheng Qiu,G.H. Su,Jian Deng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.1

Pool boiling heat transfer is widely applied in nuclear engineering fields. The influence of heating surface orientation on the pool boiling heat transfer has received extensive attention. In this study, the heating surface with different roughness was adopted to conduct pool boiling experiments at different inclination angles. Based on the boiling curves and bubble images, the effects of inclination angle on the pool boiling heat transfer and critical heat flux were analyzed. When the inclination angle was bigger than 90°, the bubble size increased with the increase of inclination angle. Both the bubble departure frequency and critical heat flux decreased as the inclination angle increased. The existing theoretical models about pool boiling heat transfer and critical heat flux were compared. From the perspective of bubble agitation model and Hot/Dry spot model, the experimental phenomena could be explained reasonably. The enlargement of bubble not only could enhance the agitation of nearby liquid but also would cause the bubble to stay longer on the heating surface. Consequently, the effect of inclination angle on the pool boiling heat transfer was not conspicuous. With the increase of inclination angle, the rewetting of heating surface became much more difficult. It has negative effect on the critical heat flux. This work provides experimental data basis for heat transfer and CHF performance of pool boiling.

Growth, Clonability, and Radiation Resistance of Esophageal Carcinoma-derived Stem-like Cells

Li, Jian-Cheng,Liu, Di,Yang, Yan,Wang, Xiao-Ying,Pan, Ding-Long,Qiu, Zi-Dan,Su, Ying,Pan, Jian-Ji Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: To separate/enrich tumor stem-like cells from the human esophageal carcinoma cell line OE-19 by using serum-free suspension culture and to identify their biological characteristics and radiation resistance. Methods: OE-19 cells were cultivated using adherent and suspension culture methods. The tumor stem-like phenotype of CD44 expression was detected using flow cytometry. We examined growth characteristics, cloning capacity in soft agar, and radiation resistance of 2 groups of cells. Results: Suspended cells in serum-free medium formed spheres that were enriched for CD44 expression. CD44 was expressed in 62.5% of suspended cells, but only in 11.7% of adherent cells. The suspended cells had greater capacity for proliferation and colony formation in soft agar than the adherent cells. When the suspended and adherent cells were irradiated at 5 Gy, 10 Gy, or 15 Gy, the proportion of CD44+ suspended cells strongly and weakly positive for CD44 was 77.8%, 66.5%, 57.5%; and 21.7%, 31.6%, 41.4%, respectively. In contrast, the proportion of CD44+ adherent cells strongly positive for CD44 was 18.9%, 14.%, and 9.95%, respectively. When the irradiation dose was increased to 30 Gy, the survival of the suspended and adherent cells was significantly reduced, and viable CD44+ cells were not detected. Conclusion: Suspended cell spheres generated from OE-19 esophageal carcinoma cells in serum-free stem medium are enriched in tumor stem-like cells. CD44 may be a marker for these cells.