Life history responses of the cabbage beetle Colaphellus bowringi to temperature change

Jian‐Jun Tang,Hai-Min HE,Ting GENG,Shu FU,Fang-Sen XUE 한국곤충학회 2016 Entomological Research Vol.46 No.6

Temperature is considered one of the most important mediators of phenotypic plasticity in ectotherms. Here, we investigated life history traits of the cabbage beetle, Colaphellus bowringi Baly (Coleoptera: Chrysomelidae), at a wide range of temperatures (16, 19, 22, 24, 26 and 28°C). The larval and pupal times were significantly decreased with increasing rearing temperature and growth rate was positively correlated with temperature. However, the relationship between body size and rearing temperature in C. bowringi did not follow the temperature–size rule; both males and females reached the highest body weight at 19°C. Females were significantly larger than males at all temperatures. Male pupae lost significantly more weight at metamorphosis compared to females. However, diapausing males gained significantly higher weight after feeding compared to diapausing females at higher temperatures of 22, 24, 26 and 28°C. Body weight tended to decrease with increasing rearing temperature, whereas sexual size dimorphism (SSD) tended to increase with increasing rearing temperature; thus, Rensch's rule is upheld. The degree to which SSD changed with temperature varied with different development stages. SSD was lowest in pupae, highest in newly emerged adults and intermediate in diapausing adults.

An Inscribed Guoshulǚ Bell from the Freer Collections and its Relevant Issues

FANG Jian jun 아시아음악학회 2001 Asian Musicology Vol.18 No.-

In Freer Gallery of Art, there is an inscribed bronze bell. According to its inscription, it is called Guoshulǚ bell, namely, the owner of the bell was Guoshulǚ. This paper examines the inscriptions, compares with other provenanced Guoshulǚ bells, discusses the combination and acoustical property of Guoshulǚ bells. In conclusion, the author thinks that Guoshulǚ bell in Freer Gallery of Art can be dated to Western Zhou dynasty (ca. 8th-9th century B.C.), but it is a true bell with the false inscription, the complete set of Guoshulǚ bells has 8 pieces, the tones of the bells can be formed with a tetrachord in Chinese yu mode.

An Inscribed Guoshulǚ Bell from the Freer Collections and its Relevant Issues

FANG Jian jun 아시아음악학회 2011 Asian Musicology Vol.18 No.-

Juvenile hormone-mediated reproduction induced by a sublethal concentration of imidacloprid in Cyrtorhinus lividipennis (Hemiptera: Miridae)

Jun Zhu,Yao Li,Ying-Ying Tang,Jian-Qi Liu,Yi-Yu Chen,Zhongxian LV,Fang Liu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

The Hemipteran predator, Cyrtorhinus lividipennis, feeds on the eggs and nymphs of rice planthoppers and leafhoppers. We previously demonstrated that sublethal concentrations of imidacloprid stimulated the reproduction of C. lividipennis. Considering the essential roles of juvenile hormone (JH) in insect reproduction, we speculated that sublethal concentrations of imidacloprid may stimulate the reproduction of C. lividipennis by regulating JH level. To test this, we cloned C. lividipennis JH acid methyl transferase (ClJHAMT) and JH esterase (ClJHE), which are responsible for JH biosynthesis and degradation genes, respectively. We then knocked down ClJHAMT by injecting dsRNA into C. lividipennis nymphs and found that emerging female adults exhibited 88.8% lower expression of the vitellogenin gene (ClVg) and the number of eggs was reduced by 41.5% as compared with controls. Silencing ClJHE increased ClVg mRNA expression by 275.0% but did not affect fecundity. A sublethal concentration of imidacloprid (LC 20 ) increased the JH titer in females by 35.3% and 60.6% at 24 and 48 h post-emergence, respectively. In treatments containing both imidacloprid and dsJHAMT, the silencing of CLJHAMT reduced the number of eggs produced by adult females by 21.4% as compared to the control (imidacloprid + dsGFP). Our results indicated that sublethal concentration of imidacloprid may induce C. lividipennis reproduction by upregulating JH level via JHAMT. The finding could provide valuable information for improved integration of C. lividipennis and insecticides in pest management.

Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells

Jun-Cheng Guo,Yi-Jun Yang,Jin-Fang Zheng,Jian-Quan Zhang,Min Guo,Xiang Yang,Xiang-Ling Jiang,Li Xiang,You Li,Huang Ping,Liu Zhuo 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths, but its molecular mechanisms are not yet well characterized. Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, including that of HCC. However, the role of homeobox A11 antisense (HOXA11-AS) in determining HCC stem cell characteristics remains to be explained; hence, this study aimed to investigate the effects of HOXA11-AS on HCC stem cell characteristics. Initially, the expression patterns of HOXA11-AS and HOXA11 in HCC tissues, cells, and stem cells were determined. HCC stem cells, successfully sorted from Hep3B and Huh7 cells, were transfected with short hairpin or overexpression plasmids for HOXA11-AS or HOXA11 overexpression and depletion, with an aim to study the influences of these mediators on the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo. Additionally, the potential relationship and the regulatory mechanisms that link HOXA11-AS, HOXA11, and the Wnt signaling pathway were explored through treatment with Dickkopf-1 (a Wnt signaling pathway inhibitor). HCC stem cells showed high expression of HOXA11-AS and low expression of HOXA11. Both HOXA11-AS silencing and HOXA11 overexpression suppressed the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo, as evidenced by the decreased expression of cancer stem cell surface markers (CD133 and CD44) and stemness-related transcription factors (Nanog, Sox2, and Oct4). Moreover, silencing HOXA11-AS inactivated the Wnt signaling pathway by decreasing the methylation level of the HOXA11 promoter, thereby inhibiting HCC stem cell characteristics. Collectively, this study suggested that HOXA11-AS silencing exerts an antitumor effect, suppressing HCC development via Wnt signaling pathway inactivation by decreasing the methylation level of the HOXA11 promoter.

Prevalence of Spina Bifida Occulta and Its Relationship With Overactive Bladder in Middle-Aged and Elderly Chinese People

Jun Wei Wu,Yu Rong Xing,Yi Bo Wen,Tian Fang Li,Quan De Feng,Xiao Ping Shang,Yun Long Li,Jin Jin Feng,Xin Xin Wang,Rong Qun Zhai,Xiang Fei He,Tao Chen,Xin Jian Liu,Jian Guo Wen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.2

Purpose: To investigate the prevalence of spina bifida occulta (SBO) and its relationship with the presence of overactive bladder (OAB) in middle-aged and elderly people in China. Methods: A cross-sectional community-based survey was carried out at 7 communities in Zhengzhou City, China from December 15, 2013 to June 10, 2014, where residents aged over 40 years were randomly selected to participate. All of the participants underwent lumbosacral radiographic analysis and relevant laboratory tests. A questionnaire including basic information, past medical history and present illness, and the OAB symptom score was filled out by all participants. Chi-square tests and logistic regression were used for data analysis with a P-value of <0.05 denoting statistical significance. Results: A total of 1,061 subjects were qualified for the final statistical analysis (58.8±11.7 years; male, 471 [44.4%]; female, 590 [55.6%]). The overall prevalence of SBO was 15.1% (160 of 1,061): 18.3% (86 of 471) in men and 12.5% (74 of 590) in women. Among these subjects, 13.7% (145 of 1,061) had OAB: 13.2% (62 of 471) in men and 14.1% (83 of 590) in women. The results of logistic regression showed that age, SBO, history of cerebral infarction (HCI), and constipation were risk factors for OAB (P<0.05), while sex, history of childhood enuresis (HCE), body mass index (BMI), and diabetes mellitus (DM) were not (P>0.05). In men, age, SBO, and constipation were risk factors for OAB (P<0.05), while HCE, BMI, DM, HCI, and benign prostate hyperplasia were not (P>0.05). In women, age, SBO, and HCI were risk factors for OAB (P<0.05), while HCE, BMI, DM, vaginal delivery, and constipation were not (P>0.05). Conclusions: The prevalence of SBO is high and it is related to OAB in middle-aged and elderly people in China.

Identification and characterization of a novel endoglucanase (CMCase) isolated from the larval gut of Bombyx mori

Ru-Jian Ma,Chun-Yan Wang,Yan-Wei Liu,Thasma Raman Sivakumar,Zi-Xu Ren,Ying Fang,Jun-Qiang Jia,Zhong-Zheng Gui 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1

While screening for cellulase-producing fungi from insect gut, a fungus with high endoglucanase (carboxymethylcellulase; CMCase) activitywas isolated fromthe larval gut of Bombyx mori. Based onmorphological characteristicsand using an 18S rRNA-based molecular phylogenetic approach, the fungus, strain BMC-2, was identified as aMucor sp. expressing a novel alkalotolerant cellulase. The maximum production of cellulase by the BMC-2 strainwas observed at 55°C and pH8.0. The CMCase activity was inhibited by Cu2+ N Na+ N Zn2+ N Mg2+ N Ba2+, andinduced by Ca2+, Mn2+, Fe2+, and K+.

Sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge

Zhang, Jun,Zheng, Jin-Wei,Cho, Byung Cheol,Hwang, Chung Yeon,Fang, Chengxiang,He, Jian,Li, Shun-Peng Microbiology Society 2012 International journal of systematic and evolutiona Vol.62 No.3

<P>A Gram-negative, non-motile, non-spore-forming, non-flagellated rod capable of degrading cypermethrin, designated LQY-18<SUP>T</SUP>, was isolated from activated sludge of a wastewater treatment plant in China. Strain LQY-18<SUP>T</SUP> grew at 8-40 °C (optimum 30 °C), at pH 5.0-10.0 (optimum pH 7.0) and with 0-5 % (w/v) NaCl (optimum 1 %). The predominant menaquinone was MK-7 (97 %) and the major fatty acids were summed feature 3 (comprising C16 : 1ω6<I>c</I> and/or C16 : 1ω7<I>c</I>), iso-C15 : 0 and iso-C17 : 0 3-OH. The DNA G+C content was 40.3 mol%. Phylogenetic analysis revealed that the isolate belonged to the genus <I>Sphingobacterium</I> of the phylum <I>Bacteroidetes</I> and showed low 16S rRNA gene sequence similarity with recognized members of the genus <I>Sphingobacterium</I>. The closest neighbour was <I>Sphingobacterium mizutaii</I> ATCC 33299<SUP>T</SUP> (92.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, genetic and phylogenetic data, strain LQY-18<SUP>T</SUP> ( = ACCC 05410<SUP>T</SUP> = CCTCC AB 2010005<SUP>T</SUP> = KCTC 23009<SUP>T</SUP>) should be classified as a representative of a novel species of the genus <I>Sphingobacterium</I>, for which the name <I>Sphingobacterium wenxiniae</I> sp. nov. is proposed.</P>

Experimental Study and Numerical Simulation of Precast Shear Wall with Rabbet-Unbonded Horizontal Connection

Chong-fang Sun,Shu-ting Liang,Xiao-jun Zhu,Hu Li,Jian-min Guo,Gang Li,Ya-min Song,Dong-yue Wu 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.1

This paper reports the results of a seismic performance study of a precast shear wall with a new horizontal connection. The new connection is the rabbet-unbonded horizontal connection, which is composed of rabbets and unbonded rebar segments. The rabbets are used to improve the shear capacity and prevent slippage of the connection, and the unbonded rebar segments are used to improve the ductility and energy dissipation. Three specimens were tested with different parameters under cyclic quasi-static loading. The test results showed that the specimen with a larger unbonded level had a richer hysteresis curve, larger ductility, larger energy dissipation, and slightly smaller bearing capacity. Moreover, in relation to the stiffness degradation, in the initial stage, the specimen with a larger unbonded level had a smaller stiffness, whereas in the last stage, the stiffnesses were similar regardless of the unbonded level. A parameter analysis using a finite element model proved that the ductility and energy dissipation of a shear wall with the rabbet-unbonded horizontal connection increased with the unbonded length and level. In addition, when the axial compression ratio increased, the bearing capacity increased, but the load-displacement curves decreased more rapidly. It was concluded that the unbonded length and unbonded level could effectively improve the ductility and energy dissipation of a shear wall. However, they should not be too large under high pressure, and the design suggestions for the new connection need further research considering other factors.

Association Between XRCC1 Gene Polymorphisms and Risk of Glioma Development: A Meta-analysis

Sun, Jian-Ying,Zhang, Chun-Yang,Zhang, Zhen-Jun,Dong, Yan-Fang,Zhang, An-Long,Wang, Zhi-Wei,Mei, Xiao-Long Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: Previous studies of the association between X-ray cross-complementing group 1 (XRCC1) gene polymorphisms and the gliomas risk have yielded conflicting results, and thus a meta-analysis was performed to provide a more accurate estimation. Methods: A computerized literature search of 5 electronic databases was conducted to identify the relevant studies. Fixed or random effect models were selected based on the heterogeneity test. Publication bias was estimated using Begg's funnel plots and Egger's regression test. Results: A total of 11 studies (3,810 cases and 6,079 controls), 7 studies (2,928 cases and 5,048 controls), and 4 studies (1,461 cases and 2,593 controls) were finally included in the analyses of the association between XRCC1 Arg399Gln, Arg194Trp, and Arg280His polymorphisms and glioma risk, respectively. The pooled results showed that GlnGln carriage was associated with moderately increased risk of gliomas in Asians (GlnGln vs. ArgArg, OR=1.490, 95%CI 1.031-2.153; GlnGln/ArgGln vs. ArgArg, OR=1.321, 95%CI 1.037-1.684), whereas a marginal association was revealed in Caucasians. For the Arg194Trp polymorphism, although a significant association was shown in the homozygous genotype comparisons (TrpTrp vs. ArgArg, OR = 2.209, 95%CI 1.398-2.945), no significant link was found on subgroup analysis stratified by ethnicity. With regard to the Arg280His polymorphism, no significant association was found in each comparison. No particular study was found to significantly influence the pooled results, and no potential publication bias was detected. Conclusions: This meta-analysis suggested that the XRCC1 Arg399Gln polymorphism is moderately associated with increased risk of gliomas in Asians, while Arg194Trp and Arg280His polymorphisms demonstrated no significant influence. Due to the limited studies and the potential confounders, further studies are needed to confirm these results.