Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

Xie, Jia-jun,Chen, Xu,Niu, Jin,Wang, Jun,Wang, Yun,Liu, Qiang-qiang The Korean Society of Fisheries and Aquatic Scienc 2017 Fisheries and Aquatic Sciences Vol.20 No.4

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and $H_2O_2$ supplementation were applied (control group: without both astaxanthin and $H_2O_2$ treated; $H_2O_2$ group: just with $H_2O_2$ treated; $H_2O_2$ + AST group: with both astaxanthin and $H_2O_2$treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of $H_2O_2$ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation ($H_2O_2$ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from $H_2O_2$ (P < 0.05), but it could be raised by astaxanthin supplementation ($H_2O_2$ vs $H_2O_2$ + AST), and the malondialdehyde (MDA) was significant higher in $H_2O_2$ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

( Jia-jun Xie ),( Xu Chen ),( Jin Niu ),( Jun Wang ),( Yun Wang ),( Qiang-qiang Liu ) 한국수산과학회 2017 Fisheries and Aquatic Sciences Vol.20 No.2

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and H₂O₂ supplementation were applied (control group: without both astaxanthin and H₂O₂ treated; H₂O₂ group: just with H₂O₂ treated; H₂O₂ + AST group: with both astaxanthin and H₂O₂ treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of H₂O₂ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation (H₂O₂ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from H₂O₂ (P < 0.05), but it could be raised by astaxanthin supplementation (H₂O₂ vs H₂O₂ + AST), and the malondialdehyde (MDA) was significant higher in H₂O₂ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

Comparison of Liver Transplantation and Liver Resection for Hepatocellular Carcinoma Patients with Portal Vein Tumor Thrombus Type I and Type II

Jia-Yu Lv,Ning-Ning Zhang,Ya-Wei Du,Ying Wu,Tian-Qiang Song,Ya-Min Zhang,Yan Qu,Yu-Xin Liu,Jie Gu,Ze-Yu Wang,Yi-Bo Qiu,Bing Yang,Da-Zhi Tian,Qing-Jun Guo,Li Zhang,Ji-San Sun,Yan Xie,Zheng-Lu Wang,Xin 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.1

Purpose: The aim of this study was to compare the efficacy of liver transplantation (LT) and liver resection (LR) for hepatocellularcarcinoma (HCC) patients with portal vein tumor thrombus (PVTT) and to investigate risk factors affecting prognosis. Materials and Methods: A total of 94 HCC patients with PVTT type I (segmental PVTT) and PVTT type II (lobar PVTT) were involvedand divided into LR (n=47) and LT groups (n=47). Recurrence-free survival (RFS) and overall survival (OS) were comparedbefore and after inverse probability of treatment weighting (IPTW). Prognostic factors for RFS and OS were explored. Results: Two treatment groups were well-balanced using IPTW. In the entire cohort, LT provided a better prognosis than LR. Among patients with PVTT type I, RFS was better with LT (p=0.039); OS was not different significantly between LT and LR(p=0.093). In subgroup analysis of PVTT type I patients with α-fetoprotein (AFP) levels >200 ng/mL, LT elicited significantly longermedian RFS (18.0 months vs. 2.1 months, p=0.022) and relatively longer median OS time (23.6 months vs. 9.8 months, p=0.065). Among patients with PVTT type II, no significant differences in RFS and OS were found between LT and LR (p=0.115 and 0.335,respectively). Multivariate analyses showed treatment allocation (LR), tumor size (>5 cm), AFP and aspartate aminotransferase(AST) levels to be risk factors of RFS and treatment allocation (LR), AFP and AST as risk factors for OS. Conclusion: LT appeared to afford a better prognosis for HCC with PVTT type I than LR, especially in patients with AFP levels>200 ng/mL.

Evolution of the Mir-155 Family and Possible Targets in Cancers and the Immune System

Xie, Guang-Bing,Liu, Wei-Jia,Pan, Zhi-Jun,Cheng, Tian-Yin,Luo, Chao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

The mir-155 family is not only involved in a diversity of cancers, but also as a regulator of the immune system. However, the evolutionary history of this family is still unclear. The present study indicates that mir-155 evolved independently with lineage-specific gain of miRNAs. In addition, arm switching has occurred in the mir-155 family, and alternative splicing could produce two different lengths of ancestral sequences, implying the alternative splicing can also drive evolution for intragenic miRNAs. Here we screened validated target genes and immunity-related proteins, followed by analyzation of the mir-155 family function by high-throughput methods like the gene ontology (GO) and Kyoto Eneyclopedin of Genes and Genemes (KEGG) pathway enrichment analysis. The high-throughput analysis showed that the CCND1 and EGFR genes were outstanding in being significantly enriched, and the target genes cebpb and VCAM1 and the protein SMAD2 were also vital in mir-155-related immune reponse activities. Therefore, we conclude that the mir-155 family is highly conserved in evolution, and CCND1 and EGFR genes might be potential targets of mir-155 with regard to progress of cancers, while the cebpb and VCAM1 genes and the protein SMAD2 might be key factors in the mir-155 regulated immune activities.

Research on the Power Sharing Control and Stability of VSGs

Xie, Dong,Zang, Da-Jin,Gao, Peng,Wang, Jun-Jia The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.2

Aiming at the deficiencies of power sharing control performances when a traditional droop control is adopted for microgrid inverters, this paper proposes a microgrid inverter power sharing control strategy based on a virtual synchronous generator. This control method simulates the electromechanical transient characteristics of a synchronous generator in a power system by an ontology algorithm and the control laws of a synchronous generator by control over the speed governor and excitation regulator. As a result, that the microgrid system is able to effectively retain the stability of the voltage and frequency, and the power sharing precision of the microgrid inverter is improved. Based on an analysis of stability of a microgrid system controlled by a virtual synchronous generator, design thoughts are provided for further improvement of the power sharing precision of inverters. The simulation results shows that when the virtual synchronous generator based control strategy was adopted, the power sharing performances of microgrid inverters are improved more obviously than those using the droop control strategy.

Spectroscopic Determination of the Species Fractions and the Power Profiles of the Diagnostic Neutral Beam on the HT-7 Tokamak

Jia Fu,Yuejiang Shi,Yingying Li,Fudi Wang,Sheng Liu,Jian Zhang,Jun Li,Yiyyun Huang,Yuanlai Xie,Zhimin Liu,Chundong Hu,Chundong Hu,DNB Team,William Rowan,He Huang 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.5

A diagnostic neutral beam (DNB) has been installed on the HT-7 tokamak for the measurement of charge exchange recombination spectroscopy (CXRS). The H_α-light Doppler shift spectroscopy from the drifted duct is measured to determine the components of the neutral beam. The fractions of neutral beam species are investigated under a wide range of arc voltages and extraction high voltages of the beam aimed to the optimized species fractions for the CXRS applications. A magnet ring is used to improve the magnetic property of the ion source. The result shows that the full-energy component fractions increase from 19 to 25 percent with optimization of the beam operation, but with a dramatic increase of the water component. There are nine optical fiber channels observing one section of the beam simultaneously for this spectroscopy, which provides information of the power profiles of the beam. The full width at half maximum (FWHM) of the beam profile is 8 cm, as measured by using the spectroscopy.

Intensified solvent extraction and separation of cobalt from Ni-rich leaching solution in impinging stream-rotating packed bed contactor

Chang Jun,Jia Fukang,Mumford Kathryn A.,Yang Xiaohong,Xie Zinan 한국자원공학회 2020 Geosystem engineering Vol.23 No.5

This work describes the separation of Ni and Co from a real feed solution in chloride media with high Ni content. The extractant used was P507 (2-ethylhexyl phosphoric acid-2-ethylhexyl ester) in a laboratory scale Impinging Stream–Rotating Packed Bed (IS-RPB) contactor. Key variables including feed solution pH, extractant concentration, saponification degree, high gravity level and total volumetric flow rate of fluids amongst other variables were examined. A McCabe–Thiele diagram of the extraction stage predicted that at a total volumetric flow rate of 90 L/h, feed solution pH of 5, saponified P507 concentration of 25% and high gravity of 83, almost 99% of cobalt, would be extracted within three stages. With regard to the scrubbing results, the co-extracted nickel was scrubbed efficiently from the loaded organic solution using a mixture of 0.02 M CoCl2 and 0.5 M hydrochloric acid solution, The purified Co solution was achieved after stripping the loaded-organic phase with 1 M HCl. The maximum value of the overall volumetric mass transfer coefficient for the IS-RPB extractor was found to be 0.16 s−1 which was superior to that of conventional extractors such as mixer-settler and centrifugal extractors, providing strong confidence for its industrial application.

Research on the Power Sharing Control and Stability of VSGs

Dong Xie,Da-Jin Zang,Peng Gao,Jun-Jia Wang 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.2

Aiming at the deficiencies of power sharing control performances when a traditional droop control is adopted for microgrid inverters, this paper proposes a microgrid inverter power sharing control strategy based on a virtual synchronous generator. This control method simulates the electromechanical transient characteristics of a synchronous generator in a power system by an ontology algorithm and the control laws of a synchronous generator by control over the speed governor and excitation regulator. As a result, that the microgrid system is able to effectively retain the stability of the voltage and frequency, and the power sharing precision of the microgrid inverter is improved. Based on an analysis of stability of a microgrid system controlled by a virtual synchronous generator, design thoughts are provided for further improvement of the power sharing precision of inverters. The simulation results shows that when the virtual synchronous generator based control strategy was adopted, the power sharing performances of microgrid inverters are improved more obviously than those using the droop control strategy.

JCAD deficiency attenuates activation of hepatic stellate cells and cholestatic fibrosis

Li Xie,Hui Chen,Li Zhang,Yue Ma,Yuan Zhou,Yong-Yu Yang,Chang Liu,Yu-Li Wang,Ya-Jun Yan,Jia Ding,Xiao Teng,Qiang Yang,Xiu-Ping Liu,Jian Wu 대한간학회 2024 Clinical and Molecular Hepatology(대한간학회지) Vol.30 No.2

Background/Aims: Cholestatic liver diseases including primary biliary cholangitis (PBC) are associated with active hepatic fibrogenesis, which ultimately progresses to cirrhosis. Activated hepatic stellate cells (HSCs) are the main fibrogenic effectors in response to cholangiocyte damage. JCAD regulates cell proliferation and malignant transformation in nonalcoholic steatoheaptitis-associated hepatocellular carcinoma (NASH-HCC). However, its participation in cholestatic fibrosis has not been explored yet. Methods: Serial sections of liver tissue of PBC patients were stained with immunofluorescence. Hepatic fibrosis was induced by bile duct ligation (BDL) in wild-type (WT), global JCAD knockout mice (JCAD-KO) and HSC-specific JCAD knockout mice (HSC-JCAD-KO), and evaluated by histopathology and biochemical tests. In situ-activated HSCs isolated from BDL mice were used to determine effects of JCAD on HSC activation. Results: In consistence with staining of liver sections from PBC patients, immunofluorescent staining revealed that JCAD expression was identified in smooth muscle α-actin (α-SMA)-positive fibroblast-like cells and was significantly up-regulated in WT mice with BDL. JCAD deficiency remarkably ameliorated BDL-induced hepatic injury and fibrosis, as documented by liver hydroxyproline content, when compared to WT mice with BDL. Histopathologically, collagen deposition was dramatically reduced in both JCAD-KO and HSC-JCAD-KO mice compared to WT mice, as visualized by Trichrome staining and semi-quantitative scores. Moreover, JCAD deprivation significantly attenuated in situ HSC activation and reduced expression of fibrotic genes after BDL. Conclusions: JCAD deficiency effectively suppressed hepatic fibrosis induced by BDL in mice, and the underlying mechanisms are largely through suppressed Hippo-YAP signaling activity in HSCs.

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Qian, Jun,Li, Jing,Jia, Jian-Guang,Jin, Xin,Yu, Da-Jun,Guo, Chen-Xu,Xie, Bo,Qian, Li-Yu Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Objectives: To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. Materials and Methods: SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. Results: It was found that the proliferation of SP was significantly faster than that of NSP (P<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (P<0.05). Conclusions: With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.