갑상선 세포에서 전사보조활성인자인 CBP와 CIITA의 인터페론-감마 활성 부위에 대한 조절 : CBP and CIITA on GAS(Interferon- γ Activated Site)-Mediated Transcription in Thyroid Cells

채수흥,홍우정,김군순,조영석,김도희,김현진,구본정,송민호,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : In the previous stuides, we identified that the interferon-r activated sequence (GAS) in the 5-flanking region of rat ICAM-1 gene is major element for interferon-r-inducible expression of the gene in rat thyroid cells, FRTL-5. We here, investigated the role of transcriptional coactivators, CBP(CREB binding protein) and CⅡTA (class Ⅱ transactivator) in the modulation of the activity of GAS which could interacts with signal transducers and activators of transcription-1 and 3 (STAT1 and STAT3). Methods : The expression of CBP RNA and protein were quantitated in FRTL-5 after stimulation with interferon-r (IFN-r), thyroid stimulating hormone (TSH), forskolin and methimazole. Direct association of CBP with STAT were analyzed by immunoprecipitation. The transcriptional roles of CBP and CⅡTA in the regulation of GAS were assessed by the cotransfection with their expression vectors with reporters; 5-deletion constructs of rat ICAM-1 promoter or 8xGAS-luc constructs, into FRTL-5 thyroid cells. Results : The level of CBP RNA and protein were not changed by the treatment with TSH, IFN-r, forskolin and methimazole in FRTL-5, FRT and BRL liver cells. The CBP could be directly associated with STAT1. Furthermore, the overexpression of CBP significantly increases the both promoter activities; rat ICAM-1 gene promoter which has GAS element and 8xGAS-luc cassette constructs. However, the cotransfection of CⅡTA decreased the constitutive and CBP-mediated transactivation of rat ICAM-1 promoter and 8xGAS-luc cassette constructs. Conclusion : We identified that the two tanscriptional coactivators; CBP and CⅡTA has differential roles in the regulation of transcriptional activity of GAS drived promoter. CBP increases the GAS activity through the direct binding with STAT1, but CⅡTA inhibited the CBP-mediated transactivation of GAS activity.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

지속가능성 지표의 설정과 응용에 관한 연구

김민정,김종오,이창호 慶尙大學校生産技術硏究所 1998 生産技術硏究所論文集 Vol.14 No.-

The purpose of this study was to develope the Sustainability index representing the environmental conservation and development balance of 71 cities in Korea. The Sustainability index was set up from 3 major factors ; human-living environment, social-economy environment, and natural environment. Each factor was divided into some subfactors and total 9 subfactors were considered. Also, each subfactor contained some variables reflecting sustainability concepts and total 46 variables was selected. Principle components analysis performed repeatedly in each subfactor group and first principle component was used to develope weighting value of each variables for sustainability index. Using sustainability index, some cities in Korea could be compared and evaluated.

은퇴기간에 따른 노후자금지속가능성 분석

김민정,박광수 동의대학교 경제경영전략연구소 2011 經濟經營硏究 Vol.6 No.2

In this study, we aim at presenting a Financial Asset Withdrawal Strategy that can be applied to Korean retirees’household economy. Financial Asset Withdrawal Strategy means a strategy to manage the assets accumulated before retirement in an asset portfolio, aiming at preventing the old age fund from being exhausted before the end of the retirement period, even if a certain amount is withdrawn during that period. That is, this strategy is to find out the rate of withdrawal(the rate of the amount withdrawn in the first year of retirement to the amount of the old age fund in the first year of retirement) that will not exhaust the old age fund before the end of the retirement period, even if the withdrawal from each of the portfolio compositions is continued for a certain period. The ratio of withdrawal in this case is called Sustainable Initial Withdrawal Rate(SIWR) and the largest value is called the Maximum Sustainable Initial Withdrawal Rate(MaxSIWR). In 0 percent probability of failing, MaxSIWR30 is 4.2% with a 0/100 bond/stock allocation. In 1%, 10%, 20%, MaxSIWR30 is 4.4%, 4.9%, 5.9% with 10/90, 20-40/80-60, 50-60/50-40 bond/stock allocation, respectively. If retirees plan longer retirement periods in order to consider longevity risks, they should enhance the weight of investments in stocks and should use a smaller MaxSIWR.

초고층 주상복합 아파트 거주 후 평가에 관한 연구 : S 초고층 아파트 거주자 설문을 통하여

배정익,안병욱,김상민 대한건축학회 2004 대한건축학회논문집 Vol.20 No.9

Recently, super high-rise apartments having more than 30 floors have been constructing 10 town. This study investigated residents' preferences and satisfactions focusing on architectural design factors of super high-rise apartment Statistical methods, anova, correspondence analysis and Pearson correspondence analysis were used to find the factors that affect the preference As a result, the following findings were obtained 1) Generally, the residents are satisfied with the living environment of high-rise apartment housing 2) The result shows that the residents prefer in order of "transportation and location", "maintenance", "environmentally friendly plan", "automated equipment", "new concept design", and "economics" 3) Satisfaction level for "daylighting" and "View" is highly influenced by the floor level 4) The "circulation" affects more on the preference of site planning than any other elements 5) The "prevention of noise" affects more on the preference of interior planning than any other elements 6) The "Air-conditioning" affects more on the preference of interior environmental planning than any other elements

외상 후 뇌 지방색전증 1예

문정미,소정일,김용권,류진호,허탁,서정진,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Post-traumatic fat embolism was first reported by Zenker in 1862, Von Bergmann reported the first clinical diagnosis of the fat embolism syndrome in 1873. Fat embolism has been associated with traumatic or non-traumatic disorders. Fat embolization after long bone trauma is probably common as a subclinical event. The diagnosis of fat embolism syndrome is based on the patient's history, is supported by clinical signs of pulmonary, cerebral, and cutaneous dysfunction, and is confirmed by the demonstration of arterial hypoxemia in the abscence of other disorders. Two different mechanisms cause fat to embolize: direct entry of deposit fat into the blood stream and agglutination of endogenous or exogenous plasma fat. MRI can detect a cerebral fat embolism with a higher sensitivity than cerebral CT. We report a case of post-traumatic cerebral fat embolism without pulmonary involvement, and we present a review of the literature. A 16-year girl had a traffic accident and pelvic bone fracture. Forty eight hours later severe trauma become stuporous without a focal neurological deficit. The patient received supportive therapy, and her condition improved throughout her hospital course. She was discharged with good condition after a 30-day hospital stay.

三日酒와 三日 基以內로 빚어지는 우리나라의 速成酒 : Ⅱ. 찹쌀로 빚은 速成酒 Ⅱ. About Brewing from Glutinous Rice

錢精日,申敏子,崔榮辰,金賢淑,李惠靜,鄭在洪,白淑殷 동아시아식생활학회 1999 東아시아食生活學會誌 Vol.9 No.3

This study was carried out to understand the brewing and processing methods of rapid-brew alcoholic beverage. In the contents of 45 classical records, there were 68 items about rapid-brew alcoholic beverage and single and double brew alcoholic beverage. Those alcoholic beverage of 68 items above, were made from green grown at that time-rice, glutinous rice, regular rice. Selected 38 items among those brewages, alcoholics from glutinous rice, were distributed into 4 patterns. Four patterns, grounded on rapid-brewing and processing methods, were set one thing to another and analyzed.

대학생의 개인재무관리역량

이슬기, 박현정, 김민정 충북대학교 생활과학연구소 2012 생활과학연구논총 Vol.16 No.1

The purpose of this study is to measure financial competency of students in Chung-buk national university. The financial competency included financial attitude, financial knowledge, and financial skills. The major findings are as follows: First, as the financial management competency of university students, their attitude towards financial management was 75 points, knowledge in financial management was 68.5 points, and financial management skill was 59.9 points. Second, male students showed higher scores than female students in all areas. Third, students in the fields of medicine, education, and laws showed higher levels of financial management knowledge yet their financial management skill was low. Forth, students who receive allowances from parents showed high points of attitude towards financial management yet their financial management skill was low. Those who receive allowances from people that are not parents showed lower points in the attitude towards financial management or the knowledge than those who do not receive allowances. Fifth, students who spend money they earned showed higher points in every area than other students’ points. Finally students who received financial education showed similar or lowered points in financial management attitude and financial management knowledge with students who did not receive the financial education. However, the level of their financial management function was higher. Students who have saving accounts, periodicaldeposits, fixed deposits, a check card, or debit card showed higher levels of financial management knowledge and function that those who do not have the above items.

Quality Characteristics of Jeung-pyun Using Dry-Milled Rice Flour

Ji Won Park,Yoon Jeung Lee,Min Young Kim,Myeong Seob Song,Hyun Ah Oh,Park Gi Hoon,Sun Young Choi,Heon Sang Jeong 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10