Effect of Oral Administration of Lactobacillus plantarum HY7714 on Epidermal Hydration in Ultraviolet B-Irradiated Hairless Mice

( Jehyeon Ra ),( Dong Eun Lee ),( Sung Hwan Kim ),( Ji Woong Jeong ),( Hyung Keun Ku ),( Tae Youl Kim ),( Il Dong Choi ),( Woonhee Jeung ),( Jae Hun Sim ),( Young Tae Ahn ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.12

In this study, we evaluated the effect of Lactobacillus plantarum HY7714 on skin hydration in human dermal fibroblasts and in hairless mice. In Hs68 cells, L. plantarum HY7714 not only increased the serine palmitoyltransferase (SPT) mRNA level, but also decreased the ceramidase mRNA level. In order to confirm the hydrating effects of L. plantarum HY7714 in vivo, we orally administered vehicle or L. plantarum HY7714 at a dose of 1 × 109 CFU/day to hairless mice for 8 weeks. In hairless mice, L. plantarum HY7714 decreased UVB-induced epidermal thickness. In addition, we found that L. plantarum HY7714 administration suppressed the increase in transepidermal water loss and decrease in skin hydration, which reflects barrier function fluctuations following UV irradiation. In particular, L. plantarum HY7714 administration increased the ceramide level compared with that in the UVB group. In the experiment on SPT and ceramidase mRNA expressions, L. plantarum HY7714 administration improved the reduction in SPT mRNA levels and suppressed the increase in ceramidase mRNA levels caused by UVB in the hairless mice skins. Collectively, these results suggest that L. plantarum HY7714 can be a potential candidate for preserving skin hydration levels against UV irradiation.

Apple Pomace Extract Improves Endurance in Exercise Performance by Increasing Strength and Weight of Skeletal Muscle

정지웅,심재중,최일동,Sung Hwan Kim,Jehyeon Ra,구형근,Dong Eun Lee,Tae-Youl Kim,Woonhee Jeung,Jung-Hee Lee,이기원,허철성,Jae-Hun Sim,YOUNG-TAE AHN 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.12

Ursolic acid is a lipophilic pentacyclic triterpenoid found in many fruits and herbs and is used in several herbal folk medicines for diabetes. In this study, we evaluated the effects of apple pomace extract (APE; ursolic acid content, 183 mg/g) on skeletal muscle atrophy. To examine APE therapeutic potential in muscle atrophy, we investigated APE effects on the expression of biomarkers associated with muscle atrophy and hypertrophy. We found that APE inhibited atrophy, while inducing hypertrophy in C2C12 myotubes by decreasing the expression of atrophy-related genes and increasing the expression of hypertrophy-associated genes. The in vivo experiments using mice fed a diet with or without APE showed that APE intake increased skeletal muscle mass, as well as grip strength and exercise capacity. In addition, APE significantly improved endurance in the mice, as evidenced by increased exhaustive running time and muscle weight, and reduced the expression of the genes involved in the development of muscle atrophy. APE also decreased the concentration of serum lactate and lactate dehydrogenase, inorganic phosphate, and creatinine, the indicators of accumulated fatigue and exercise-induced stress. These results suggest that APE may be useful as an ergogenic functional food or dietary supplement.

Clinical Evidence of Effects of Lactobacillus plantarum HY7714 on Skin Aging: A Randomized, Double Blind, Placebo-Controlled Study

( Dong Eun Lee ),( Chul Sung Huh ),( Jehyeon Ra ),( Il Dong Choi ),( Ji Woong Jeong ),( Sung Hwan Kim ),( Ja Hyun Ryu ),( Young Kyoung Seo ),( Jae Sook Koh ),( Jung Hee Lee ),( Jae Hun Sim ),( Young T 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

The beneficial effects of probiotics are now widely reported, although there are only a few studies on their anti-aging effects. We have found that Lactobacillus plantarum HY7714 (HY7714) improves skin hydration and has anti-photoaging effects, and in the present study, we have further evaluated the anti-aging effect of HY7714 via a randomized, double blind, placebo-controlled clinical trial. The trial included 110 volunteers aged 41 and 59 years who have dry skin and wrinkles. Participants took 1 × 10(10) CFU/day of HY7714 (probiotic group) or a placebo (placebo group) for 12 weeks. Skin hydration, wrinkles, skin gloss, and skin elasticity were measured every 4 weeks during the study period. There were significant increases in the skin water content in the face (p < 0.01) and hands (p < 0.05) at week 12 in the probiotic group. Transepidermal water loss decreased significantly in both groups at weeks 4, 8, and 12 (p < 0.001 compared with baseline), and was suppressed to a greater extent in the face and forearm in the probiotic group at week 12. Volunteers in the probiotic group had a significant reduction in wrinkle depth at week 12, and skin gloss was also significantly improved by week 12. Finally, skin elasticity in the probiotic group improved by 13.17% (p < 0.05 vs. controls) after 4 weeks and by 21.73% (p < 0.01 vs. controls) after 12 weeks. These findings are preliminary confirmation of the anti-aging benefit to the skin of L. plantarum HY7714 as a nutricosmetic agent.

Effects of gut microbiota on the pharmacokinetics of protopanaxadiol ginsenosides Rd, Rg3, F2, and compound K in healthy volunteers treated orally with red ginseng

Kim, Jeon-Kyung,Choi, Min Sun,Jeung, Woonhee,Ra, Jehyeon,Yoo, Hye Hyun,Kim, Dong-Hyun The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.4

Background: It is well recognized that gut microbiota is involved in the biotransformation of ginsenosides by converting the polar ginsenosides to nonpolar bioactive ginsenosides. However, the roles of the gut microbiota on the pharmacokinetics of ginsenosides in humans have not yet been fully elucidated. Methods: Red ginseng (RG) or fermented red ginseng was orally administered to 34 healthy Korean volunteers, and the serum concentrations of the ginsenosides were determined using liquid chromatography-tandem mass spectrometry. In addition, the fecal ginsenoside Rd- and compound K (CK)eforming activities were measured. Then, the correlations between the pharmacokinetic profiles of the ginsenosides and the fecal ginsenoside-metabolizing activities were investigated. Results: For the RG group, the area under the serum concentratione-time curve values of ginsenosides Rd, F2, Rg3, and CK were 8.20 ± 11.95 ng·h/mL, 4.54 ± 3.70 ng·h/mL, 36.40 ± 19.68 ng·h/mL, and 40.30 ± 29.83 ng·h/mL, respectively. For the fermented red ginseng group, the the area under curve from zero to infinity (AUC<SUB>∞</SUB>) values of ginsenosides Rd, F2, Rg3, and CK were 187.90 ± 95.87 ng·h/mL, 30.24 ± 41.87 ng·h/mL, 28.68 ± 14.27 ng·h/mL, and 137.01 ± 96.16 ng·h/mL, respectively. The fecal CK-forming activities of the healthy volunteers were generally proportional to their ginsenoside Rd-eforming activities. The area under the serum concentration-time curve value of CK exhibited an obvious positive correlation (r = 0.566, p < 0.01) with the fecal CK-forming activity. Conclusion: The gut microbiota may play an important role in the bioavailability of the nonpolar RG ginsenosides by affecting the biotransformation of the ginsenosides.

Effects of gut microbiota on the pharmacokinetics of protopanaxadiol ginsenosides Rd, Rg3, F2, and compound K in healthy volunteers treated orally with red ginseng

Jeon-Kyung Kim,Min Sun Choi,Woonhee Jeung,Jehyeon Ra,Hye Hyun Yoo,DONG-HYUNKIM 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4

Background: It is well recognized that gut microbiota is involved in the biotransformation of ginsenosidesby converting the polar ginsenosides to nonpolar bioactive ginsenosides. However, the roles of thegut microbiota on the pharmacokinetics of ginsenosides in humans have not yet been fully elucidated. Methods: Red ginseng (RG) or fermented red ginseng was orally administered to 34 healthy Koreanvolunteers, and the serum concentrations of the ginsenosides were determined using liquidchromatographyetandem mass spectrometry. In addition, the fecal ginsenoside Rde and compound K(CK)eforming activities were measured. Then, the correlations between the pharmacokinetic profiles ofthe ginsenosides and the fecal ginsenosideemetabolizing activities were investigated. Results: For the RG group, the area under the serum concentrationetime curve values of ginsenosides Rd,F2, Rg3, and CK were 8.20 11.95 ng$h/mL, 4.54 3.70 ng$h/mL, 36.40 19.68 ng$h/mL, and40.30 29.83 ng$h/mL, respectively. For the fermented red ginseng group, the the area under curve fromzero to infinity (AUCN) values of ginsenosides Rd, F2, Rg3, and CK were 187.90 95.87 ng$h/mL,30.24 41.87 ng$h/mL, 28.68 14.27 ng$h/mL, and 137.01 96.16 ng$h/mL, respectively. The fecal CKformingactivities of the healthy volunteers were generally proportional to their ginsenoside Rdeformingactivities. The area under the serum concentrationetime curve value of CK exhibited an obvious positivecorrelation (r ¼ 0.566, p < 0.01) with the fecal CK-forming activity. Conclusion: The gut microbiota may play an important role in the bioavailability of the nonpolar RGginsenosides by affecting the biotransformation of the ginsenosides.

Inhibitory effect of<i>Agrimoniae Herba</i>on lipopolysaccharide-induced nitric oxide and proinflammatory cytokine production in BV2 microglial cells

Bae, Hyunsu,Kim, Hye-Jeoung,Shin, Minkyu,Lee, Hyejung,Yin, Chang Shik,Ra, Jehyeon,Kim, Jinju Informa UK (TaylorFrancis) 2010 Neurological research Vol.32 No.suppl1

<P>OBJECTIVES: Agrimoniae Herba has been used as an anti-inflammatory agent in traditional medicine. Nitric oxide and proinflammatory cytokines produced by activated microglia may be a possible etiological factor of neurodegenerative disorders. We evaluated whether Agrimoniae Herba could have an anti-inflammatory effect on lipopolysaccharide-induced BV2 microglial cells. METHODS: The effects of Agrimoniae Herba on lipopolysaccharide-induced nitric oxide and proinflammatory cytokine production in BV2 microglial cells were evaluated by nitric oxide assay, enzyme-linked immunosorbent assay and western blotting. RESULTS: Agrimoniae Herba had no cytotoxicity and suppressed lipopolysaccharide-induced nitric oxide production in BV2 microglial cells. Agrimoniae Herba also suppressed lipopolysaccharide-induced production of proinflammatory cytokines such as tumor necrosis factor, interleukin 1 beta and interleukin 6 in a dose-dependent manner. Agrimoniae Herba inhibited the expression of inducible nitric oxide synthase. DISCUSSION: Taken together, these findings indicate that Agrimoniae Herba may be used as a form of pharmaceutical acupuncture therapy in the treatment of brain inflammation.</P>

Hepatoprotective Effect of Lactiplantibacillus plantarum DSR330 in Mice with High Fat Diet-Induced Nonalcoholic Fatty Liver Disease

( Na-kyoung Lee ),( Yunjung Lee ),( Da-soul Shin ),( Jehyeon Ra ),( Yong-min Choi ),( Byung Hee Ryu ),( Jinhyeuk Lee ),( Eunju Park ),( Hyun-dong Paik ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.2

Lactiplantibacillus plantarum DSR330 (DSR330) has been examined for its antimicrobials production and probiotics. In this study, the hepatoprotective effects of DSR330 were examined against nonalcoholic fatty liver disease (NAFLD) in a high-fat diet (HFD)-fed C57BL/6 mouse model. To induce the development of fatty liver, a HFD was administered for five weeks, and then silymarin (positive control) or DSR330 (10⁸ or 10⁹ CFU/day) was administered along with the HFD for seven weeks. DSR330 significantly decreased body weight and altered serum and hepatic lipid profiles, including a reduction in triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels compared to those in the HFD group. DSR330 significantly alleviated HFD-related hepatic injury by inducing morphological changes and reducing the levels of biomarkers, including AST, ALT, and ALP.Additionally, DSR330 alleviated the expression of SREBP-1c, ACC1, FAS, ACO, PPARα, and CPT-1 in liver cells. Insulin and leptin levels were decreased by DSR330 compared to those observed in the HFD group. However, adiponectin levels were increased, similar to those observed in the ND group. These results demonstrate that L. plantarum DSR330 inhibited HFD-induced hepatic steatosis in mice with NAFLD by modulating various signaling pathways. Hence, the use of probiotics can lead to hepatoprotective effects.