제초제(propanil. butachlor) 저항성 잡초 선발 방법에 관한 연구

정성엽,김영미,박재현,이재현,김학윤,이인중,신동현,김길웅 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

This study was conducted to develop a simple selection method for herbicide-resistance weeds. Two methods, designated "seedling method" and "stem node method" were employed for screening of barnyardgrass against propanil and butachlor. In the seedling method, shoot and root growth of barnyardgrass were significantly inhibited at quarter of the recommended herbicide rate, while in the stem node method, the similar inhibition was obtained at half of the recommended rate. Thus, it was concluded that the seedling method is more simple and quick method to evaluate response of barnyardgrass to propanil and butachlor compared to stem node method.

비장에서의 악성 림프종

이상숙,임태진,김인호,추중엽 啓明大學校 醫科大學 1996 계명의대학술지 Vol.15 No.1

We experienced a case of malignant lymphoma of the spleen on 69-year-old male patient with huge splenomegaly. The spleen represented diffuse proliferation of large lymphocytes with plasmacytoid feature by microscope and strong positively stained to B-cell marker for immunohistochemical stain and finally diagnosed as malignant lymphoma, large cell, immunoblastic, diffuse(B-cell type). So we reported this case and reviewed the related literature briefly.

유방확대술 후 실리콘 삽입물 파열의 치험례

이정엽,홍인표,심영기,이세일 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.1

Silicone prosthesis is well known as relatively acceptable material since Cronin had introduced at 1963. But it can't be a perfect material due to complications such as capsular contracture, prosthesis rupture. We experienced wto cases of prosthesis rupture with siliconoma of breast and the one was identified with history of closed capsulotomy and the other without trauma history. With review of the reported cases, we should consider followings 1. Silicone gel prosthesis can be ruptured with closed capsulotomy or without trauma history. 2. Ruptured prosthesis and silicone gel must be removed for prevention of progressive complications as soon as possible prosthesis rupture is noted.

제 3형 구상돌기 골절의 수술적 치료

전인호,민우기,오창욱,경희수,박병철,김풍택,인주철,이정엽 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적: 8례의 제 3형 척골 구상돌기 골절에 대해 수술적 치료 후 임상적 방사선학적 결과를 보고하고자 한다. 대상 및 방법 : 8명의 구상돌기 골절에 대한 후향적 연구를 시행하였다. 대상은 모두 남자였으며 평균 나이는 33세였다. 3례에서 척골 구상돌기 단독 골절이었으며 2례에서 주관절 탈구가, 2례에서 요골 두 및 경부 골절이, 1례에서 내측 측부인대 손상이 동반되었다. 전례에서 전방 도달법을 통해 유관나사를 이용한 관혈적 정복 및 내고정이 시행되었다. 평균 추시 기간은 31개월 이었으며 (24~60개월), Mayo Elbow Performance Score로 임상적 결과를 판정하였다. 결과 : 최근 추시에서 평균의 능동적 주관절 가동범위는 105°였다. 평균의 Mayo Elbow Performance Score는 76.9점이었다(50~95). 1례에서 최우수, 4례에서 우수, 2례에서 양호, 1례에서 불량의 결과를 보였다. 결론 : 제 3형의 척골 구상돌기 골절에서 조기의 관혈적 정복 및 안정적인 내고정으로 신뢰할 만한 치료를 제공하였으며, 손상 주관절의 동반된 인대손상과 복합골절은 중요한 예후인자로 간주되었다. Purpose: To review the clinical results of eight cases of typeⅢ coronoid process fractures which were treated operatively. Materials and Methods : Eight patients with coronoid type Ⅲ were reviewed retrospectively. All were men with an average age of 33. There were three isolated fractures, two elbow dislocations, two radial head and neck fractures, and one medial collateral ligament rupture. Open reduction and internal fixation through anterior approach with canulated screws was used. The patients were followed up for a mean of 31 months(24 to 60). Results: Average active elbow joint motion at the most recent follow up was 105°. The average Mayo Elbow Performance Score was 76.9 (50 to 95). There was one excellent result, four good, two fair, and one poor. Conclusion: Early open reduction and stable internal fixation provided a reliable method for the treatment of typeⅢ coronoid process fractures. Any associated ligament injuries to the elbow and fracture comninution were considered as important prognostic factors.

<i>TP53</i> Mutations in Korean Patients with Non-small Cell Lung Cancer

Lee, Eung Bae,Jin, Guang,Lee, Shin Yup,Park, Ji Young,Kim, Min Jung,Choi, Jin Eun,Jeon, Hyo Sung,Cha, Seung Ick,Cho, Sukki,Kim, Chang Ho,Park, Tae-In,Jung, Tae Hoon,Son, Ji-Woong,Park, Jae Yong The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.5

<P>Although <I>TP53</I> mutations have been widely studied in lung cancer, the majority of studies have focused on exons 5-8 of the gene. In addition, <I>TP53</I> mutations in Korean patients with lung cancers have not been investigated. We searched for mutations in the entire coding exons, including splice sites of the gene, in Korean patients with non-small cell lung cancer (NSCLC). Mutations of the gene were determined by direct sequencing in 176 NSCLCs. Sixty-nine mutations (62 different mutations) were identified in 65 tumors. Of the 62 mutations, 12 were novel mutations. <I>TP53</I> mutations were more frequent in males, ever-smokers and squamous cell carcinomas than in females, never-smokers and adenocarcinomas, respectively (all comparisons, <I>P</I><0.001). Missense mutations were most common (52.2%), but frameshift, nonsense, and splice-site mutations were frequently observed at frequencies of 18.8%, 15.9% and 10.1%, respectively. Of the 69 mutations, 9 (13.0%) were found in the oligomerization domain. In addition, the proportion of mutations in the oligomerization domain was significantly higher in adenocarcinomas than in squamous cell carcinomas (23.5% vs. 2.9%, <I>P</I>=0.01). Our study provides clinical and molecular characteristics of <I>TP53</I> mutations in Korean patients with NSCLCs.</P>

Bone marrow findings in severe fever with thrombocytopenia syndrome: prominent haemophagocytosis and its implication in haemophagocytic lymphohistiocytosis

Kim, Namhee,Kim, Kye-Hyung,Lee, Su Jin,Park, Sang-Hyuk,Kim, In-Suk,Lee, Eun Yup,Yi, Jongyoun British Medical Association 2016 Journal of clinical pathology Vol.69 No.6

<P><B>Aims</B></P><P>Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by the SFTS virus; primary manifestations are fever, thrombocytopenia, leukopenia and gastrointestinal symptoms. Before an aetiological diagnosis is made, SFTS patients can undergo bone marrow examination due to cytopenias. Although several studies have reported on bone marrow examination in SFTS patients, most do not provide adequate details. Bone marrow findings in SFTS patients were investigated in this study.</P><P><B>Methods</B></P><P>An observational study was conducted in SFTS patients who were hospitalised between 2013 and 2014 in two university hospitals in South Korea. Patients were included in the study if SFTS was confirmed by real-time PCR for the SFTS virus and a bone marrow examination was conducted. The morphologic findings of the bone marrow samples were reviewed.</P><P><B>Results</B></P><P>Three cases met the study inclusion criteria. One patient died of multiple organ failure. Haemophagocytosis was evident in the bone marrow samples of all three patients. Histiocytic hyperplasia and haemophagocytosis were more pronounced in the fatal case. One patient was diagnosed as having haemophagocytic lymphohistiocytosis.</P><P><B>Conclusions</B></P><P>Haemophagocytosis in the bone marrow of SFTS patients may be common. In SFTS endemic areas, SFTS should be one of the differential diagnoses of fever of unknown origin with haemophagocytosis in the bone marrow.</P>

한국 소아의 폐기능 검사치에 관한 연구

박종무,이하백,설인준,장순섭,이수엽 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Pulmonary function testing differs in children and adults. And difficulty is known to exist in performing accurate testings in children due to many factors including cooperation and because volume changes occur from birth through the period of growth to adulthood. In spite of its obvious importance, possibly due to the difficulties mentioned, the data on the normal values of pulmonary function test (PFT) in Korean children are extremely scarce, and therefore, currently interpretation of the attempted PFTs becomes less meaningful without proper reference normal values. This study was conducted to attempt to investigate the reliability of PFT currently available in children, to determine the normal range for the results of PFTs in Korean children, and to analyze the PFTs results in the study population. A total of 189 Korean children, between 6 and 15 years of age, was subjected to PFTs at Hanyang University Hospital during the period of 3 years from March, 1983 to June, 1986, in this study. The results of this study are as follows: 1. In 125 boys studied, height ranged from 113.0 to 179.0cm, weight from 17.3 to 63.0 Kg, and body surface area (BSA) from 0.75 to 1.80㎡. In 64 girls studied, height ranged from 108.5 to 155.0 cm, weight from 17.0 to 50.0 Kg, and BSA from 0.72 to 1.50㎡. 2. FVC test showing MEFV curve, MVV, and Spirometry were performed on each study subject. Numbers, means, and standard deviations of each group of body and girls at 10㎝ interval of height, were obtained for FVC, FEV₁, FEV₁/FVC, ??, PF, ??, ??, ??, TV, ERV, IRV, IC and VC. Boys and girls were divided by 7 and 6 groups of height, respectively, and the means of each measurement of both sexes increased along with increase in height as a whole, except for FEV₁/FVC. 3. Prediction equations and correlation coefficients on height were obtained in boys and girls for FVC, FEV₁, ??, PF, ?? and ??, Correlation coefficients of predication equations in boys range from 0.69 to 0.92; those in girls form 0.67 to 0.91, and those for FVC and FEV₁in both sexes were observed to be the highest among them. 4. Comparing the results in both sexes, the predicted values of FVC, FEV₁, PF, and ?? Of boys were higher than those of girls as a whole except under about 115㎝ in height. However, the predicted values of ?? And ?? Of girls were higher than those of boys over all heights. 5. In comparison with other races, the predicted values of FVC and FEV₁of boys were lower than those of Mexican Americans and whites, but higher than those of blacks over all heights. And the predicted values of FEF were lower than those of Mexican Americans and whites over all heights, but higher than those of blacks as a whole. The predicted values of FVC and FEV₁of girls were lower than those of whites and Mexican Americans over all height, but higher than those of blacks as a whole. And the predicted values of ?? were lower than those of Mexican Americans and whites, but higher than those of blacks over all heights.

Genetic association of MYH genes with hereditary hearing loss in Korea

Kim, Sang-Joo,Lee, Seokwon,Park, Hong-Joon,Kang, Tae-Hun,Sagong, Borum,Baek, Jeong-In,Oh, Se-Kyung,Choi, Jae Young,Lee, Kyu-Yup,Kim, Un-Kyung Elsevier 2016 Gene Vol.591 No.1

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Myosin is a key protein involved in regulating the shape and motility of cells. The <I>MYH9</I> and <I>MYH14</I> genes, which encode non-muscle myosin heavy chain IIA (NMMHC II-A) and IIC (NMMHC II-C), respectively, are expressed in the inner ear. These myosin genes are known to be associated with autosomal dominant non-syndromic hearing loss (ADNSHL); however, genetic studies in patients with ADNSHL in Korea have rarely been reported.</P> <P><B>Methods</B></P> <P>We analyzed the <I>MYH9</I> and <I>MYH14</I> genes in 75 Korean patients with ADNSHL.</P> <P><B>Results</B></P> <P>We identified 4 possible pathogenic variants: a novel variant p.F1303L and 2 previously reported variants (p.R1730C and p.R1785C) in the <I>MYH9</I> gene, and a novel variant p.A1868T in the <I>MYH14</I> gene. All the variants were located in the myosin tail domain, which is essential for the interaction of myosin with actin. These variants were predicted to be possibly pathogenic by functional prediction tools and were absent in 100 unrelated normal controls.</P> <P><B>Conclusion</B></P> <P>These results suggest that all the variants identified in this study have a strong potential to affect the structural stability and/or function of non-muscle myosin in the inner ear, which might lead to ADNSHL. This study establishes the link between the genotype and development of ADNSHL and contributes to the establishment of Korean database for hereditary hearing loss.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We performed genetic analysis of <I>MYH</I> genes in Korean patients with ADNSHL. </LI> <LI> We found three and one possibly pathogenic variants in <I>MYH9</I> and <I>MYH14</I>, respectively. </LI> <LI> Our research will improve understanding of relations between MYH genes and ADNSHL. </LI> </UL> </P>

Incidences and Prognostic Impact of c-KIT, WT1, CEBPA, and CBL Mutations, and Mutations Associated With Epigenetic Modification in Core Binding Factor Acute Myeloid Leukemia: A Multicenter Study in a Korean Population

Sang Hyuk Park,Hyun Ji Lee,In-Suk Kim,Jeong-Eun Kang,Eun Yup Lee,Hyeoung-Joon Kim,Yeo-Kyeoung Kim,Jong-Ho Won,Soo-Mee Bang,Hawk Kim,Moo-Kon Song,Joo Seop Chung,Ho Jin Shin 대한진단검사의학회 2015 Annals of Laboratory Medicine Vol.35 No.3

Background: To identify potential molecular prognostic markers in core binding factor (CBF) AML, we analyzed incidences and prognostic impacts of mutations in c-KIT, WT1, CEBPA, CBL, and a number of epigenetic genes in CBF AML. Methods: Seventy one and 21 AML patients with t(8;21) and inv(16) were enrolled in this study, respectively. NPM1, CEBPA, c-KIT, IDH1/2, DNMT3A, EZH2, WT1, and CBL mutations were analyzed by direct sequencing. Patients were categorized with respect to c-KIT and WT1 mutation status, and both clinical features and prognoses were compared. Results: The incidences of FLT3 internal tandem duplication (ITD), NPM1, CEBPA, IDH1/2, DNMT3A, EZH2, and CBL mutations were low (≤5%) in CBF AML patients. However, c-KIT and WT1 mutations occurred frequently (10.9% and 13.8%, respectively). t(8;21) patients with c-KIT mutations showed significantly shorter overall survival (OS) and disease free survival (DFS) periods than those without mutations (P<0.001, for both); however, although the limited number of t(8;21) patients were analyzed, WT1 mutation status did not affect prognosis significantly. Relapse or death during follow-up occurred more frequently in t(8;21) patients carrying c-KIT mutations than in those without the mutation, although the difference was significant only in a specific patient subgroup with no WT1 mutations (P=0.014). Conclusions: The incidences of mutations in epigenetic genes are very low in CBF AML; however, c-KIT and WT1 mutations occur more frequently than others. The poor prognostic impact of c-KIT mutation in t(8;21) AML patients only applies in a specific patient subgroup without WT1 mutations. The prognostic impact of WT1 mutation in CBF AML is not evident and further investigation is required.

Non-invasive stem cell tracking in hindlimb ischemia animal model using bio-orthogonal copper-free click chemistry

Lee, Si Yeon,Lee, Sangmin,Lee, Jangwook,Yhee, Ji Young,Yoon, Hwa In,Park, Soon-Jung,Koo, Heebeom,Moon, Sung-Hwan,Lee, Hyukjin,Cho, Yong Woo,Kang, Sun Woong,Lee, Sang-Yup,Kim, Kwangmeyung Elsevier 2016 Biochemical and biophysical research communication Vol.479 No.4

<P><B>Abstract</B></P> <P>Labeling of stem cells aims to distinguish transplanted cells from host cells, understand <I>in vivo</I> fate of transplanted cells, particularly important in stem cell therapy. Adipose-derived mesenchymal stem cells (ASCs) are considered as an emerging therapeutic option for tissue regeneration, but much remains to be understood regarding the <I>in vivo</I> evidence. In this study, a simple and efficient cell labeling method for labeling and tracking of stem cells was developed based on bio-orthogonal copper-free click chemistry, and it was applied in a mouse hindlimb ischemia model. The human ASCs were treated with tetra-acetylated <I>N</I>-azidoacetyl-<SMALL>D</SMALL>-mannosamine (Ac<SUB>4</SUB>ManNAz) to generate glycoprotein with unnatural azide groups on the cell surface, and the generated azide groups were fluorescently labeled by specific binding of dibenzylcyclooctyne-conjugated Cy5 (DBCO-Cy5). The safe and long-term labeling of the hASCs by this method was first investigated <I>in vitro</I>. Then the DBCO-Cy5-hASCs were transplanted into the hindlimb ischemia mice model, and we could monitor and track <I>in vivo</I> fate of the cells using optical imaging system. We could clearly observe the migration potent of the hASCs toward the ischemic lesion. This approach to design and tailor new method for labeling of stem cells may be useful to provide better understanding on the therapeutic effects of transplanted stem cells into the target diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new method was proposed for labeling and tracking of stem cells. </LI> <LI> This method enables safe and long-term monitoring of stem cells <I>in vivo</I>. </LI> <LI> We observed the migration of the stem cells toward the ischemic lesion. </LI> </UL> </P>