석회화치성낭양 변화를 동반한 법랑모세포섬유치아종

권혁록,한진우,이진호,최항문,박인우,이석근 대한구강악안면방사선학회 2001 Imaging Science in Dentistry Vol.31 No.3

Thirteen-year-old girl complaining of the swelling and pain on the left midface visited our dental hospital. On the radiogaphic examination, well-defined radiolucent lesion with hyperostotic border was found in the left maxilla accompanying with the external root resorption of the involved teeth and the displaced second molar. CT showed calcified bodies, thinning of hard palate, inferior orbital wall and lateral wall of nasal fossa, and thinning and perforation of the buccal plate of the maxilla. Enucleation and curettage of the lesion and nasoantrostomy was carried out and histopathologic examination mainly showed a solid tumor tissue composed of odontogenic epithelium and pulp tissues admixed with dentin and enamel formation. And some part of reduced follicular epithelium of tooth germ showed a change mimicking calcifying odontogenic cyst, Taken together, we concluded the lesion is an ameloblastic fibro-odontoma with a change of calcifying odontogenic cyst. (Korean J Oral Maxillofac Radiol 2001; 31 : 181-4)

국내 살모넬라 분리주에서 spvR 유전자 분포와 단일 뉴클레오티드 다형 연구

권혁준,박경윤,박주연,박용호,김선중,유한상 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 지금까지 병원성 살모넬라를 확인하기 위해 분자량이 큰 플라스미드의 존재 유무를 확인하여 왔으나 살모넬라 혈청형 및 검출 방법에 따라 양성율에 큰 차이를 보이고 있고, 일부 병원성 살모넬라 분리주에서 병원성 플라스미드가 관찰되지 않아 spv 유전자들의 유무에 대한 아른 확인방법이 필요하다. 본 실험은 국내 동물 유래 주요 살모넬라 혈청형에서 spvR 유전자의 분포를 알아보고, 병원성 살모넬라 분자진단을 위한 분자표지로써의 가치를 평가하기 위해 실행하였다. 재료 및 방법 : 국내 가축 유래 S. lyphimurium (ST, 26주), S. enteritidis (SE, 10주), S. pullorum (SP, 40주), S. gallinarum (SG, 53주)에 대해 GenBank에 등록된 ST, SE, SP의 spvR 유전자를 비교하여 ST와 SE 간에 차이를 보이는 single nucleotide polymorphism (SNP, 625번 뉴클레오티드)을 포함하도록 시발자를 합성하여 집락-PCR을 수행하였다. 증폭 산물(194bp)은 자동 염기서열장치를 이용하여 염기서열을 결정하였고, 제한효소인 MseI을 사용하여 PCR-RFLP를 실시하였다. 결과 : 집락-중합효소연쇄반응 결과 SP, SG, SE의 모든 (100%) 분리주에서 특이 증폭산물이 검출되었으나 ST의 경우 19주(73%)에서만 증폭되었다. 특히 병원성 관련 플라스미드가 관찰되지 않았던 SP 4주에서 특이적인 증폭산물이 검출되었다. 염기서열 분석결과 SE, SG, SP는 625번 뉴클레오티드에 아데닌을, ST는 구아닌을 가지고 있어 PCR-RFLP를 이용하여 쉽게 구분할 수 있었다. 결 론 : spvR에 대한 집락-중합효소연쇄 반응법은 병원성 플라스미드 검출법보다 SE, SP, SG 병원성 주를 신속하게 검출하는데 유용하고, 625번 뉴클레오티드의 SNP는 ST와 SE, SG, SP를 구분하는 분자표지로 사용될 수 있을 것으로 기대되며, ST에 비해 SE는 진화적으로 SG와 SP에 가까운 것으로 생각되었다. Background : The Salmonella virulence plasmid (spv) genes (spvR, A, B, C and D) on the large virulence plasmids of pathogenic Salmonella serotypes can replace the virulence of the whole plasmid. Recently, virulence plasmid-negative pathogenic Salmonella isolates were isolated. However, positive rates of spv genes among Korean Salmonella serotypes have been obscure. spv genes are conserved in compared to other virulence genes but there are single nucleotide polymorphisms (SNPs) conserved in only certain serotype. Such SNPs are useful for differentiation and understanding evolution of certain serotypes. Materials and Methods : Salmonella serotypes isolated from live stocks [Salmonella typhimurium (ST, 26), S. enteritidis (SE, 10), S. gallinarum (SG, 40) and S. pullorum (SP, 53)] were used for colony-PCR. A primer set covering single nucleotide polymorphism (SNP) at 625th nucleotide of spvR was designed. The nucleotide sequences of amplicons were determined by cyclic sequencing method and RFLP was performed by using MseI. Results : All isolates of SE, SG and SP, including four plasmid-negative isolates, showed specific amplicons but not all of ST (19/26, 73%) were positive to spvR. Based on the nucleotide sequence of 625th nucleotide and PCR-RFLP, SE, SG and SP [A(625)] and ST [G(625)] could be differentiated. Conclusion : spvR can be used as a molecular marker to detect virulent SE, SG, SP and the SNP may be useful for differentiation of SE, SG, SP and ST. According to the SNP study SE may be evolutionarily closer to SG and SP than ST.

WDM-PON Link에서 upstream, transmission 성능 향상에 대한 연구

권혁춘,정구익,하상진,한상국 연세대학교 전파통신연구소 2001 電波通信論文誌 Vol.5 No.1

본 논문에서는 파장 분할 WDM-PON 구조를 제안하였으며, CW source를 가지고 Fabry-Perot laser에 injection 시키는 방법을 사용하여 4 channel 155Mbps를 20km upstream 전송하여 분석해 보았다. 제안된 WDM-PON 시스템에서의 이 injection locking 방법은 약 -7dBm ∼ -4dBm 정도의 높은 출력 파워를 제공하였고, 155Mbps 전송시 BER=10-9에서 receiver sensitivity 는 -38dBm정도를 유지하였다. 또한 제안된 구조에서 CW source로 파장 분할된 LED와 FP laser를 사용하여 각각에 대한 시스템에서의 성능을 비교 분석해 보았다. In this paper, we propose a spectrally sliced WDM-PON architecture and analyze the upstream transmission of 4 channels 155Mbps signals by 25km using an injection locking method of Fabry-Perot laser as a CW source. For a proposed WDM-PON system, This injection locking method provides high output power of -7dBm ∼ -4dBm and satisfies receiver sensitivity -38dBm for 10-9 Bit Error Rate for 155Mbps transmission. Also, we compare performances using spectrum-sliced LED and FP laser respectively as CW source for a proposed WDM-FON system.

불안정한 바닥위에서 발목각도가 기립균형에 미치는 영향

이한숙,권혁철 한국전문물리치료학회 1997 한국전문물리치료학회지 Vol.4 No.3

항혈청 투여에 따른 돼지 유행성 설사병 예방효과 : Ⅱ.임상증상, 병리조직학적 검사, 면역조직학적 검사 Ⅱ.Clinical sign, Histopathological Lesion and Immunohistochemical Finding

지영철,한정희,권혁무,정현규,이함희 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (PEDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Clinical signs and gross, histopathological lesion and immunohistochemical findings were examined. The results obtained were as follows; 1. In clinical signs, piglets of the control group appeared the typical signs of severe watery diarrhea, depression and anorexia but piglets of the PEDV antiserum treated group recovered progressively. In mortality, control group showd 75%, but PEDV antiserum treated group showed 16.7%, respectively. 2. In gross findings, piglets of the control group appeared the typical findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 3. In histopathological findings, piglets of the control group appeared the typical findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 4. In immunohistochemical findings, piglets of the PEDV antiserum treated group showed more intensive in reaction for IgG and IgG than those of control group. The recation for IgA was stronger than that of IgG. It was concluded that oral administration of PEDV antiserum to piglets was effective in preventing PEDV infection and reduced their mortality.

치수염의 임상 증상과 치수내 Lekotriene B4의 농도에 관한 연구

임성삼,권혁춘,윤수한 大韓齒科保存學會 1996 Restorative Dentistry & Endodontics Vol.21 No.1

항혈청 투여에 따른 돼지 전염성 설사병 예방효과 : Ⅰ.혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅱ.Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (REDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Serum antibody titers aginst PEDV were examined by serum neutralization (SN) test, dectection for PEDV or PEDV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the PEDV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of PEDV in feces and small intestines by RT-PCR were 26.2% and 16.7% in PEDV antiserum treated group and 48.1% and 75.0% in control group, respectively. 3. The detection rate of PEDV antigen in the small intestine by IFA were 0% in PEDV antiserum treated group and 50.0% in control group, respectively. It was concluded that oral administration of antiserum against PEDV to piglets was effective in preventing PEDV infection.

항혈청 투여에 따른 돼지 전염성 위장염 예방효과 : Ⅰ. 혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅰ. Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against transmissible gastroenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 5 hrs, 24 hrs and 36 hrs after birth. five piglets adiminstered the antiserum were experimentally infected with TGEV at four-day-old. Control group were four piglets infected with TGEV only. Serum antibody titers against TGEV were examined by serum neutralization (SN) test, dectection for TGEV or TGEV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the TGEV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of TGEV in feces and small intestines by RT-PCR were 24.5% and 20.0% in TGEV antiserum treated group and 44.0% and 75.0% in control group, respectively. 3. The detection rate of TGEV antigen in the small intestine by IFA were 26.7% in TGEV antiserum treated group and 75.0% in control group, respectively. It was concluded that oral administration of antiserum against TGEV to piglets was effective in preventing TGEV infection.

임신 생식관련인자와 비만위험

장기홍,권혁찬,이득주,유희석,이은주,한인권 대한비만학회 1995 The Korean journal of obesity Vol.4 No.1

It is thought Omt there is a close relationship between obesity and pregnancy, but there are not any concrete studies in Korea. Therefore, a retrospective study was conducted to evaluate how factors surrounding pregnancies affect the body mass index. 385 women who had no history of disease or medication as a cause of obesity were randomly selected, and factors vlated to pregnancy vi ere compared between a normal body mass index group and a group where the body mass index was more thsn one standard deviation from the average. The average age of the study group was 44,25 years(S.SS) and the average body mass index was 23.09 kg/m2(3.00). 4 the obese group the average age was statistically higher, the level of education was statistically lower, but there was no statistica] difference with regard to smoking, alcohol consumption and exercise. Age showed to be statistically significant positive correiation with body mass index, but the level of education showed negative correlation. The age of first pregnancy, age of first delivery, and the mterval between menarche and first pregnancy showed statistieally significant negative correlation with the body mass index, whereas the duration of lactation, total number of pregnancies showed statistically significant positive correlation with body mass index. There was statistical1y significant more deliveries in the group where the body mass index was more than one standard deviation frvm the average, the risk for obesity was 2.5 times higher in women who had three or more deliveries compared to those who had less than three deliveries, and the risk was 1.5 times higher in women who had their first delivery before the age of 25 years compared to those over the age of 25 years. But other factors were found not to be different between the two study group and were independent of age, level of education, aleohol consumption and smoking. It can be seen from these results that pregnancy related factors are related to obesity, and that especially age, level of education, alcohol consumption and smoking independantly increase the risk for obesity. Also those women who have had more than three deliveries or who had their first pregnancy before the age of 25 years were at risk, and there should be a formulative plan for these women. It is hoped that in-depth study of pregnancy factors contributing to obesity be determined with further study and research.

一部 公務員에서의 HBsAg의 發現率과 生化學的 檢査値에 關한 調査

김태전,권혁한 서울保健大學 1983 論文集 Vol.3 No.1