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pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리
남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2
Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.
곽혜란,한승원,이종석,김신기 한국화훼연구회 2001 화훼연구 Vol.9 No.1
The Master Gardener(MG) program is one of representative outreach program of cooperative extension at land grant Universities in U.S.A. The purpose of MG programs are primarily focused on educating community and people who want to join gardening project. MGs are valuable resources as volunteer-educators teaching environmental horticulture, increasing life quality through horticulture, community landscaping, school gardening, nutrition problems and horticultural therapy for the common-wealth or benefit of their community. MG educational programs and volunteer internships are needed to get and maintain MG title and they follow the policies and philosophies of cooperative extension. MG programs present lots of benefits by enlarge boundaries of horticulture and offer the practical educational services. To extend and activate further the value of horticulture, the MG programs should be introduced in Korea soon. MGs are regarded as new managers or primary leaders for educating horticulture, landscaping for community because of their knowledge and eagerness to help the people's advancement.
교육용 소프트웨어 개발에 적합한 하이퍼미디어 시스템에 관한 연구
박성한,정혜윤,한민규,심진석 한양대학교 공학기술연구소 1993 공학기술논문집 Vol.2 No.1
This paper presents a back-end system of hypermedia authoring system for courseware development. For the design, repersentative hypertext reference models, the Dexter model and the HAM(Hypertext Abstract Machine) which belongs to Campbell & Goodman model were analyzed. Based on those models, data objects, attributes, and operations of back-end system were designed and implemented. Because the application area of this system is on computer based education, the path mechanism is enforced for the various educational strategies. In order to reduce the user disorientation and cognitive overheads in navigating hypernetwork, filtering, backtracking and history mechanism were implemented.
Hye Suk Han,Jeong Eun Kim,Soon Kil Kwon,Hye Young Kim,Kyeong Seob Shin,Bo Ra Son,Ki Hyeong Lee,Seung Taik Kim 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.4
Hemodialysis (HD) patients continually exposed to heparin are at risk of developing heparin-induced thrombocytopenia (HIT). However, HIT is very rare in chronic HD patients with end-stage renal disease (ESRD). The authors report the case of a chronic HD patient with ESRD who developed HIT complicated by recurrent thrombocytopenia and significant bleeding episodes. A 67-year-old man with diabetic ESRD on chronic HD suddenly developed recurrent acute bleeding episodes and severe thrombocytopenia (platelet count <1.0×10(3)/uL) 2 months prior to presentation. These bleeding episodes and the thrombocytopenia always occurred 1 week after initiating HD with heparin, and improved within 1 week of discontinuing heparin. HIT was confirmed by ELISA for anti-heparin/platelet factor 4 antibody. HD was conducted successfully and thrombocytopenia did not occur after switching argatroban for heparin. This case report suggests that clinicians must consider HIT in the differential diagnosis of thrombocytopenia during maintenance HD.
Case Report : Tracheal Laceration detected by high end-tidal CO2 during endoscopic thyroidectomy
Han Suk Park,Sang Ik Lee,Hye Won Shin,Sung Uk Choi,Ji Yong Park,Hye Won Lee,Hae Ja Lim,Suk Min Yoon,Seong Ho Chang 대한마취과학회 2009 Korean Journal of Anesthesiology Vol.56 No.6
Endoscopic thyroidectomy is frequently used for cosmetic reasons, such as reducing cervical scarring. Subcutaneous gas insufflation with CO2 is needed to maintain the surgical space, and optimal surgical techniques and careful attention are required when conducting this procedure due to the limited space available for the endoscopic instruments. We report here a case of a tracheal laceration with a tear in the cuff of a reinforced tube, which was detected by an abrupt increase in end-tidal CO2 to 90 mmHg. Reintubation was achieved using a tube exchanger and the patient was effectively ventilated without complications. (Korean J Anesthesiol 2009; 56: 703~5)
Han, Hye-Suk,Lim, Sung-nam,An, Jin Young,Lee, Ki Man,Choe, Kang Hyeon,Lee, Ki Hyeong,Kim, Seung Taik,Son, Seung-Myoung,Choi, Song-Yi,Lee, Ho-chang,Lee, Ok-Jun Elsevier 2012 JOURNAL OF THORACIC ONCOLOGY Vol.7 No.2
<P>To evaluate epidermal growth factor receptor (EGFR) mutation status in lung adenocarcinoma specimens with different proportions of tumor cells using two methods with different sensitivities.</P>