최근 5년 동안 충북대학교병원의 혈액배양에서 분리된 원인균 및 향균제 감수성

신경섭 충북대학교 의과대학 충북대학교 의학연구소 2004 忠北醫大學術誌 Vol.14 No.2

연구목적: 균혈증은 심각한 합병증을 야기할 수 있으므로 혈액 검체에서 분리되는 병원성균의 종류 및 항균제 감수성의 변화양상을 주기적으로 파악하는 것은 매우 중요하다. 이에 저자들은 충북대학교 병원에 입원한 환자의 혈액에서 분리된 균의 분포 및 감수성 양상을 조사하였다. 대상 및 방법: 1999년부터 2003년까지 충북대학교병원 미생물 검사실에 의뢰된 혈액배양에서 분리된 균주를 대상으로 하였다. 균주의 동정은 통상적인 방법 및 Vitek 기기를 이용하였으며, 항균제 감수성 검사는 디스크 확산법과 Vitek 감수성 카드를 이용하였다. 결과: 5년 동안 혈액배양에서 Coagulase negative staphylococci (35.7%), Escherichia coli (14.6%), Staphylococcus aureus (11.6%), Klebsiella pneumoniae (5.9%), Pseudomonas aeruginosa (3.5%) 순으로 분리되었다. S. aureus에서 oxacillin 에 대한 내성은 59.8% 이었다. E. coli에 서 ampicil1in 및 cefotaxime 에 대해 60.4% 및 1.4% 의 내성을, K. pneumoniae에서는 99.3% 및 8.8% 의 내성을 보였다. P. aerugmosa에서 piperacillin 및 ceftazidime에 46.0% 및 35.6%의 내성을, Acinetobacter baumanii에서는 30.2% 및 3 1.7% 의 내성을 보였다. 결론: 병원균의 종류와 항균제 감수성 양상에 대해 주기적으로 파악하는 것은 내성균의 조절과 원내감염의 조기 발견 그리고 나아가 임상에서의 적절한 감염질환의 치료에 도움이 될것이다. Purpose : Because bacteremia can have serious consequences, it is of quite importance to know the current trends of species and the changing patterns of antimicrobial resistant rates of isolates from blood. So author analyzed the species and the antimicrobial susceptibility of bacteria isolated from blood at the Chungbuk National University Hospital. Materials and Methods : For the isolates from blood culture during 1999 to 2003, the identification of species was done by the conventional method and Vitek systems. The antimicrobial susceptibility tests was performed by disk diffusion and Vitek susceptibility cards. Results : Coagulase negative staphylococci (35.7%), Escherichia coli (14.6%), Stahpylococcus aureus (11.6%), Klebsiella pneumoniae (95.9%) and Pseudomonas aeruginosa (3.5%) were frequently isolated. The methicillin-resistant S. aureus was 59.8%. In E. coli and K. pneumoniae, the rate of resistance against ampicillin and cefotaxime were 60.4/1.4% and 99.3/8.8%, respectively. In P. aeruginosa and Acinetobacter baumanii, the rate of resistance against piperacillin and ceftazidime were 46.0/35.6% and 30.2/31.7%, respectively. Conclusions : The periodic analysis of distribution rates and trends of antimicrobial susceptibility can be tool to control the emergence of resistant strains and the guide to select antimicrobial agents.

자동생화학 분석기 Hitachi 7180의 평가

신경섭,주대일,김영숙 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: 저자들은 본원에 응급화학검사기기로 새로 도입된 Hitachi 7180 (Hitachi, Tokyo, Japan)의 분석 능을 보기 위하여, 정밀도, 직선성, 정확성을 평가하였으며 기존의 화학검사기기인 Hitachi 747 (Hitachi, Tokyo, Japan)과의 상관성도 평가하였다. 대상 및 방법: 포도당, 크레아티닌, blood urea nitrogen (이하 BUN), 총단백, 알부민, aspartic transaminase (이하 AST), 나트륨, 칼륨, 클로라이드 등 총 9종목에 대하여 정밀도를 측정하였으며, 포도당, 크레아티닌, BUN, AST에 대하여 직선성 및 회수율을 구하였다. 포도당, 크레아티닌, AST 및 총 빌리루빈에 대하여 Hitachi 747과 상관관계를 평가하였다 결과: 일내 정밀도는 저농도 및 고농도 정도관리 물질에서 3.05% 및 2.02% 이하의 변이계수를 보였으며, 총 정밀도는 각각 4.4%와 2.42% 이하의 변이계수를 보였다. 직선성을 평가한 모든 항목의 결정계수(r²)는 0.999 이상이었다. 회수율은 평가한 4종목에서 100.0에서 103.08% 사이이었다. Hitachi 747과 상관검사에서 측정한 4종목의 상관계수(r)는 모두 0.9986 이상이었다. 결론: Hitachi 7180은 정밀도, 직선성 및 회수율이 우수하였으며, 기존의 기기와 좋은 상관성을 보여 임상검사실에서의 적용에 유용할 것으로 사료된다. Purpose: The authors evaluated a recently introduced chemical analyzer, Hitachi 7180 (Hitachi, Tokyo, Japan) for precision, linearity, accuracy and comparability with Hitachi 747 (Hitachi, Tokyo, Japan) to see if it was suitable for the stat analyzer. Material and Methods: The precision, linearity, accuracy and comparison study were performed. For twenty day, the precision was performed for glucose, creatinine, BUN, protein, albumin, AST, sodium, potassium and chloride. The linearity and recovery rates were performed for glucose, creatinine, AST, BUN. The comparison study was performed with Hitachi 747 for glucose, creatinine, AST and total bilirubin. Results: The within-run coefficients of variations (CVs) at low and high concentrations were less than 3.05% and 2.02%, respectively. The total CVs for each groups were less than 4.4% and 2.42%, respectively. The linearity was well maintained in range of medically significant levels(r²≥0.999). The recovery rates were good for all tested items (100-103.8%). In comparison study, coefficients of correlation exceeded 0.9986 for all tests. Conclusion: The precision, linearity and accuracy of Hitachi 7180 were good. The correlation with Hitachi 747 was good. We concluded that its analyzer can be useful for clinical laboratory.

Applications of MALDI-TOF Mass Spectrometry in Clinical Microbiology

Shin, Kyeong Seob,Yum, Jonghwa 대한의생명과학회 2022 Biomedical Science Letters Vol.28 No.3

Over the past few decades, few technologies have had a greater impact on clinical microbiology laboratories than matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS). The MALDI-TOF MS is a fast, accurate, and low-cost and efficient method of microbial identification. This technology generates characteristic mass spectral fingerprints that is a unique signature for each microorganism, making it an ideal method for accurate identification at the genus and species levels of both bacterial and fastidious microorganism such as anaerobes, mycobacterium and fungi etc. In addition, MALDI-TOF MS has been successfully used in microbial subtyping and susceptibility tests such as determination of resistance genes. In this study, the authors summarized the application of MALDI-TOF MS in clinical microbiology and clinical research and explored the future of MALDI-TOF MS.

Research on the Status of Domestic Wedding Industry -Focusing on Dress, Studios, Makeup Firms-

( Kyeong Seob Shin ) 한국패션비즈니스학회 2008 패션 비즈니스 Vol.12 No.3

The purpose of this research is to analyze the overall process of the wedding industry -arranging domestic wedding firms and formulating a database related to the business. Simultaneously, with all the data in hand the research attempts to seek flaws within the wedding industry and tries to offer solutions to revitalize the industrial section. Because the list of articles is enormously expansive, for the purpose of basic research, objects have been selected according to the process presented below. Wedding-product firms have been classified within the boundaries of dresses, studios, and makeup firms; distributing channels are mainly focused on wedding planners and related-consulting firms; related departments of universities and wedding organizations are illustrated as well. Due to the unorganized system of this particular field, the research process has been conducted with materials from personal experiences, newspapers, magazines, Internet websites, documents, and interviews with wedding-related firms and organizations, and professors. As a result, over 13 subjects which formulate a market structure of over 30trillion won. However, due to lack of systemization of the industry, as it expands, numerous problems occur. Excessive competition between wedding-consulting firms and the lack of reliable education for wedding planners, unnecessary external investment and the lack of product research, false Information from the Internet puts the entire industry in a inefficient position. Organizations such as Korea Traditional clothes Industrial Union, Korea Wedding Consulting Association, Korea Martial Industrial Promotion Association(KOMIPA) etc, are made to seek for solutions. For the wedding industry to revitalize, wedding-product firms, wedding planners and consulting firms must maintain an organic relationship every season. They must systemize a proper distribution system, with wedding-product companies enhancing the quality of products, wedding planners organizing wedding plans with responsibility, and consulting firms focusing not only on profits. In order to make high-valued products, wedding-product companies must put their greatest effort in producing talented minds, and universities with related departments must do so as well. In other words, the industrial and educational section of our society must cooperate through a sophisticated system. In addition, related organizations must act to receive governmental support in order to support the industry.

Antimicrobial Susceptibility and Distribution of Salmonella spp., Shigella spp., and Vibro parahaemolyticus Isolated from Stool Specimen in a Tertiary Hospital in 2003-2010

Kyeong Seob Shin, Bo Ra Son 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

Antibiotic resistant salmonellosis has shown a recent increase, making treatment of patients with Salmonella infections difficult. This study analyzed changes in the species, serogroups, and antimicrobial susceptibility of enteric pathogens isolated from stool specimens during a period of eight years from 2003 to 2010 in a tertiary hospital. In total, 237 pathogens (1.9%) were isolated from 12,289 stool specimens, comprising 50.6% NTS and 49% Vibrio parahaemolyticus. Serogroups D, B, and C Salmonella were isolated most frequently, in that order. Of the Salmonella, 49% of the isolates were ampicillin resistant and this rate for serogroups B, C, and D Salmonella was 56.8, 64.7, and 43.5%, respectively. Overall, the rates of trimethoprim-sulfamethoxazole (SXT), chloramphenicol (CM), and ciprofloxacin resistance were 11.0, 22.0, and 0%, respectively. Two cefotaxime-resistant Salmonella were isolated in 2010. All of the V. parahaemolyticus were susceptible to SXT, CM, and tetracycline. In conclusion, NTS and V. parahaemolyticus were the major enteric pathogens isolated from stool during the eight years examined, and the rate of Salmonella resistance to antimicrobial agents showed a significant increase. Antimicrobial susceptibility testing is mandatory for proper treatment of patients with Salmonella spp. infection.

Septicemia Caused by Leuconostoc lactis with Intrinsic Resistance to Vancomycin in a Patient with Biliary Stent

Kyeong Seob Shin,Kyudong Han,Seung Bok Hong 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.3

Leuconostoc spp. is intrinsically resistant against vancomycin and rarely causes the infection in immunocompromised patients. In this report, we describe a fatal case of Leuconostoc lactis bacteremia in a patient with biliary tract stent insertion to resolve the biliary tract obstruction by multiple pseudocysts in the pancreatic head region. Leuconostic lactis isolated from the blood of the patients was confirmed by 16S rRNA sequencing and this isolate was susceptible against most antibiotics, including levofloxacin, penicillin, erythromycin and cefotaxime except vancomycin. The septic shock and multi-organ failure was abruptly progressed due to delayed use of adequate antibiotic. Using vancomycin as the empirical antibiotics in a bacteremic patient by Gram positive cocci, the treatment failures by the isolates with intrinsic resistance against vancomycin have to be considered. In addition, the prompt and accurate identification of Leuconostoc spp. are very important to select the adequate antibiotics.

Evaluation of the Antibacterial Effects of Phellinus baumii Extract on Methicillin-Resistant Staphylococcus aureus by Using Broth Microdilution Based on a Colorimetric Method

Kyeong Seob Shin,Mi Young Jo,Seung Bok Hong 대한의생명과학회 2016 Biomedical Science Letters Vol.22 No.4

The broth microdilution technique used to measure the minimal inhibitory concentration (MIC) of natural compounds against bacteria is problematic: it is difficult to visualize bacterial growth due to the color of the natural compound. Therefore, the use of a colorimetric method with a redox indicator by broth microdilution can simplify it and increase its objectivity. This study evaluated the usefulness of the colorimetric method in measuring the MIC of Phellinus baumii against methicillin-resistant Staphylococcus aureus (MRSA). The inhibition in disc diffusion method was observed from 8,192 μg/mL P. baumii in all 10 MRSA isolates examined; however, the MIC ranges of the 10 MRSA isolates was 512~2,048 μg/mL by broth microdilution using a colorimetric method; with the 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) indicator. In addition, the MIC of P. baumii by broth microdilution using MTT as indicator yielded excellent results. However, the 2, 3, 5-triphenyltetrazolium chloride (TTC) results could not be determined due to the color of the TTC indicator. The MICs of four antibiotics against MRSA using MTT or TTC were equal to those determined by visual interpretation. In conclusion, to evaluate the antibacterial effects of a natural compound, the broth microdilution technique is considered to be better than the disc diffusion method. Moreover, to resolve the problems caused by the colors of natural compounds, a colorimetric method such as that using MTT may be very valuable.

Secular Trends of Species and Antimicrobial Resistance of Blood Isolates in a Tertiary Medical Center for Ten Years: 2003~2012

Kyeong Seob Shin,Young Il Son,Yong Dae Kim,Seung Bok Hong,Je-Seop Park,Sunghyun Kim,Young-Bin Yu,Young Kwon Kim 대한의생명과학회 2014 Biomedical Science Letters Vol.20 No.2

Periodic analysis of local epidemiologic data of prevalent pathogens of blood culture can provide clinicians with relevant information to guide empirical antibiotic therapy. In this study, we analyzed a pattern of change of causative microorganisms and antimicrobial resistance at a tertiary medical center in Chungcheong province from 2003 to 2012, retrospectively. Of 70,258 blood specimens cultured, 6,063 (8.6%) were positive. Among the positive isolates, 95.9% were aerobic or facultative anaerobic bacteria, 0.1% were anaerobes, and 3.9% were fungi. Coagulase-negative Staphylococci (CoNS) (32.9%), Escherichia coli (16.7%), Staphylococcus aureus (9.1%), Klebsiella pneumoniae (6.4%), and α-hemolytic Streptococcus (5.9%) were commonly isolated bacteria, and Candida albicans (1.4%) was the most commonly isolated fungi. Enterococcus faecium progressively increased but Streptococcus pneumoniae, Acinetobacter baumannii and Proteus species gradually decreased over a period of 10 years. The multidrug-resistant microorganisms such as methicillin-resistant S. aureus (MRSA), vancomycin-resistant enterococci (VRE), cefotaxime-resistant E. coli, imipenem-resistant Pseudomonas aeruginosa (IRPA) and imipenem-resistant A. baumannii (IRAB), were significantly increased. Therefore, there is a need for a more strict control of antibiotics and a more updated guideline for the treatment of bloodstream infection.

중국(中國) 묘족(苗族) 민속의상(民俗衣裳) 이미지의 디자인 연구(硏究)

신경섭 ( Kyeong Seob Shin ) 한국패션비즈니스학회 2000 패션 비즈니스 Vol.4 No.1

The purpose of this study is to research Miao ethnic costume which have been distinctly expressed differences according tribes and areas, present fashion design reflecting image of Miao ethnic costume. Two steps was taken in order to achive this purpose. The first step was to put in order formative characteristics of Miao ethnic costume being based on the Chinese books and domestic research materials. The second step was to study formative characteristics of Miao ethnic costume in comparison 2000A/W fashion trend, illustrate and schematize fashion designs. The results of formative characteristics of Miao ethnic costume are summarized as follows 1. X or H silhouette through skirt & jacket and pants & jacket. 2. Layered look that been twisted around several items. 3. Fine pleats skirts. 4. Refined and splendid color. Black was used main color, dark blue & red purple were used coordi color, vivid color were used accent color 5. Geometrical pattern, cross stitch embroidery. 6. Various ornaments being used silver. 7. Various hair styles and hairdressers. Using above characteristics, this study presneted fusion fashion design combined images of ethnic and techno with dark blue, mustard, blue purple. The target was Forties, professional woman.

중국(中國) 패션시장(市場) 진출(進出)을 위한 방안(方案)

신경섭 ( Kyeong Seob Shin ),조규화 ( Kyu Hwa Cho ) 한국패션비즈니스학회 1998 패션 비즈니스 Vol.2 No.3