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A Review on DNA Methylation Occurring in Tissue Culture
Zhen Wang,Xin-Qiang Zheng,Yue-Rong Liang,Kwan-Jeong Song,Jian-Liang Lu 한국차학회 2015 한국차학회지 Vol.- No.S
Tissue culture is an important practice for industrial seedling production and germplasm conservation as well as transgenic operation. More and more evidences proved that DNA methylation change is usually involved in dedifferentiation, continuous cultivation and redifferentiation during tissue culture. DNA methylation variation increases with prolonging culture duration and increasing subculture times which might induce abnormal phenotype of propagules. The pattern and level of DNA methylation are significantly influenced by explants, growth regulators ents. DNA methylation change might reflect the growth and development processes and stress response of cells or tissues during tissue culture. Elucidation of epigenetic variation mechanism might help to improve tissue culture and transgene technology especially for the woody plant with high regeneration difficulty and low transformation frequency.
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Application of Building Block Approach on Crashworthiness Design of Composite Vehicular Structures
Zhen Wang,Kai Song 한국섬유공학회 2022 Fibers and polymers Vol.23 No.6
Firstly, the representative volume element (RVE) model was established at the microscale, a three-dimensionalmicromechanical material constitutive relationship was defined as the user defined material subroutine (UMAT) inABAQUS/Standard, which was able to accurately capture mechanical behaviors of both matrix and fiber materials. Constrained by periodic boundary conditions (PBCs), the RVE model can be used to predict elastic properties as well asstrength properties of the composite laminates. Then, the accuracy of the RVE model was validated by comparing the tensileresponses between predictions and experiments. Next, comparisons in the flexural responses of carbon fiber reinforcedplastic (CFRP) laminates, lateral and axial crushing behaviors of CFRP tubes between single-layer and multi-layer modelingapproaches were investigated at the mesoscale, respectively. Numerical results indicated that the single-layer model wasefficient to predict the mechanical indictors while the multi-layer method can reliably predict both mechanical indictors anddeformation patterns. Finally, the validated finite element model (FEM) and material constitutive model were employed toinvestigate the material cost and crashworthiness characteristics of the composite bumper subsystem of the electric vehicleunder the frontal crash condition at the macroscale.
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Zhen Wang,Jichang Guo,Zhiqiong Chen,Zhiqiong Chen,Lisu Yu,Yuhao Wang,Hong Rao 한국통신학회 2022 Journal of communications and networks Vol.24 No.2
This paper considers a novel scenario, where a physicallayer security issue is studied in unmanned aerial vehicles(UAVs)-assisted cognitive relay system. A secondary unmannedaerial vehicle (SUAV) relay delivers information from multiplesecondary Internet of things (IoT) devices to a secondary user(SU) under the spectrum sharing with primary users (PUs). Inthe processing of the information transmission of the SUAV relay,a secondary eavesdropper (SE) wiretaps the information transmittedby the UAV relay with imperfect location information. Inorder to confuse the SE, a friendly SUAV jammer is employedto transmit jamming signals to the SE. To prevent the SEwiretapping information as much as possible, we aim to maximizeaverage worst-case secrecy rate of the secondary relay networkby jointly optimizing robust trajectories and power of the SUAVrelay and jammer under the power, trajectories, informationcausality and multiple interference temperature (IT) thresholdconstraints. Thus, we formulate the original problem whichis a challenging non-convex problem. We propose an effectivealgorithm to solve the original problem and attain locally optimalsolution based on the successive convex approximation (SCA)technology and the alternate optimization method. Simulationsare offered to demonstrate that our proposed resource allocationscheme can effectively improve the security performance ofthe SUAV relay network in comparison with other benchmarkschemes.
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Tyrosine nitration site specificity identified by LC/MS innitrite-modified collagen type IV
Zhen Wang,David C. Paik,James P. Dillon,Elizabeth R. Gaillard 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.1
Non-enzymatic nitrite induced collagen cross-link-ing results in changes reminiscent of age-related damage and parallels the well-known model system, non-enzymatic glycation. We have recently ob-served that nitrite modification of basement mem-brane proteins can induce deleterious effects on overlying retinal pigment epithelial cells in studies relevant to age-related macular degeneration. The 3-nitro-tyrosine (3-NT) as a product of the reaction and to identify the site specificity of nitration in collagen IV, a major component of basement mem-branes. Human collagen type IV was modified via incubation with 200 mM NaNO2 (pH = 7.38) for one week at 37oC. The modified protein was prepared in 2 different ways, including acid hydrolysis and trypsin digestion for site specificity determination. The samples were analyzed by LC/MS using a C12 RP column. Site specificity was determined from tan-dem MS/MS data utilizing TurboSEQUEST software and the Swiss-Prot sequence database. 3-NT was detected in protein digests and acid hydrolysates of nitrite modified collagen IV. Positive identification with standard 3-NT was confirmed by identical Rt, λmax= 279 nm and 355 nm, and m/z= 227. Analyses of tryptic digests identified four sites of tyrosine ni-tration, α1(IV)Y348, α1(IV)Y534, α2(IV)Y327, and α2(IV)-Y1081. These sites are located in the triple- helical region of the protein and provide clues regarding potential sites for nitrite modification in collagen type IV.
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Zhen Wang,Qiyang Tan,Pengfei Shi,Ge Yang,Siyu Zhu,Guoshan Xu,Bin Wu,Jianyun Sun 국제구조공학회 2020 Smart Structures and Systems, An International Jou Vol.26 No.3
Hybrid simulation (HS) is a versatile tool for structural performance evaluation under dynamic loads. Although real structural responses are often multiple-directional owing to an eccentric mass/stiffness of the structure and/or excitations not along structural major axes, few HS in this field takes into account structural responses in multiple directions. Multi-directional loading is more challenging than uni-directional loading as there is a nonlinear transformation between actuator and specimen coordinate systems, increasing the difficulty of suppressing loading error. Moreover, redundant actuators may exist in multi-directional hybrid simulations of large-scale structures, which requires the loading strategy to contain ineffective loading of multiple actuators. To address these issues, lately a new strategy was conceived for accurate reproduction of desired displacements in bi-directional hybrid simulations (BHS), which is characterized in two features, i.e., iterative displacement command updating based on the Jacobian matrix considering nonlinear geometric relationships, and force-based control for compensating ineffective forces of redundant actuators. This paper performs performance validation and application of this new mixed loading strategy. In particular, virtual BHS considering linear and nonlinear specimen models, and the diversity of actuator properties were carried out. A validation test was implemented with a steel frame specimen. A real application of this strategy to BHS on a full-scale 2-story frame specimen was performed. Studies showed that this strategy exhibited excellent tracking performance for the measured displacements of the control point and remarkable compensation for ineffective forces of the redundant actuator. This strategy was demonstrated to be capable of accurately and effectively reproducing the desired displacements in large-scale BHS.
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Zhen Wang,,Feng Ye 경남대학교 극동문제연구소 2019 ASIAN PERSPECTIVE Vol.43 No.3
Since Sri Lanka and the People’s Republic of China established diplomatic relations in the 1950s, China has become an important partner of Sri Lanka in the latter’s cause of maintaining national independence and development. Meanwhile, Sri Lanka has been an important political and diplomatic partner for China in the Third World. Since Chinese president Xi Jinping proposed the Belt and Road Initiative (BRI) in 2013, Sri Lanka has become a new investment hot spot due to its location as a hub of the proposed 21st-Centrury Maritime Silk Road. This article provides views identifying the opportunities and potential challenges for Sino–Sri Lankan relations under the framework of China’s 21st-Century Maritime Silk Road initiative.
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Zhen Wang,Jianting Zhou,Leng Liao,Jinquan Zhang,Huabin Zhang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2
To solve the problem of bridge surface cracking caused by an uneven temperature field inside and outside the web of a concrete box structure, an automatic temperature control device applied to the beam surface of a concrete box bridge in the daytime is developed. The device, hanging on the surface of the box girder webs, consists of a radiation cooling coating and a composite shape-stabilized phase change material. With reference to a bridge in Guizhou Province, finite element analysis, short-term temperature monitoring and temperature-drop monitoring are carried out using the device. The result shows that the automatic temperature control device works well continuously. For a box girder without an automatic temperature control device, the inside and outside of the web demonstrate different sensitivities to the temperature of the external environment, with a high temperature fluctuation and a relatively high temperature stress. For a box girder with an automatic temperature control device, as the cooling coating reflects part of the solar radiation and the phase transformation of the composite shape-stabilized phase change material releases or absorbs part of the latent heat to or from the external environment, the temperature fluctuation and temperature stress of the inside and outside of the web are relatively small. When the temperature suddenly drops, the automatic temperature control device works better than under conventional weather conditions because it can reduce the external temperature by approximately 50% on the outer surface of the box girder webs.
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Zhen Wang,Yanli Zhang,Dianhai Zhang,Ziyan Ren,고창섭 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.2
The presence of direct current (dc) biased magnetic feld enhances the vibration and noise in the transformer cores due to magnetostrictive efect in the grain-oriented electrical steel laminations. Based on an Epstein frame, an experimental setup was built to measure the in-plane magnetostriction, the principal magnetostriction was computed under a dc bias, and the infuence of dc bias magnetic feld on the magnetostrictive property of electrical steel sheet was investigated. A model based on a back propagation neural network (BPNN) method assisted with the Levenberg–Marquardt algorithm was proposed to describe the magnetostrictive behaviour in the presence of dc bias. Finally, the BPNN model was verifed by comparing the measured magnetostriction and the computed one. This research is helpful to make an accurate estimation to deformation of iron core under a dc bias.
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