Effective mucosal live attenuated Salmonella vaccine by deleting phosphotransferase system component genes ptsI and crr

Yong Zhi,Shunmei Lin,A-Yeung Jang,Ki Bum Ahn,Hyun Jung Ji,Hui-Chen Guo,임상용,Ho Seong Seo 한국미생물학회 2019 The journal of microbiology Vol.57 No.1

Salmonella enterica is a major human pathogen that causes invasive non-typhoidal Salmonellosis (iNTS), resulting in significant morbidity and mortality. Although a number of pre-clinical and clinical studies have reported on the feasibility of developing a safe and effective vaccine against iNTS, there have been no licensed Salmonella vaccines available to protect against NTS strains. Vaccine formulations of highest priority for NTS are live attenuated vaccines, which can elicit effective induction of intestinal mucosal and intracellular bacteria-specific cell mediated immune responses. Since glucose is crucial for intracellular survival and replication in host cells, we constructed strains with mutations in components of the glucose uptake system, called the phosphotransferase system (PTS), and compared the relative virulence and immune responses in mice. In this study, we found that the strain with mutations in both ptsI and crr (KST0556) was the most attenuated strain among the tested strains, and proved to be highly effective in inducing a mucosal immune response that can protect against NTS infections in mice. Thus, we suggest here that KST0556 (ΔptsIΔcrr) is a potential live vaccine candidate for NTS, and may also be a candidate for a live delivery vector for heterologous antigens. Moreover, since PTS is a well-conserved glucose transporter system in both Gramnegative and Gram-positive bacteria, the ptsI and crr genes may be potential targets for creating live bacterial vectors or vaccine strains.

Usability Evaluation Method of ECDIS Human-computer Interface Based on Emotional Experience

Yongzhi Hao,Pengjun Zheng,Yibing Wang,Tianming Yan,Zhihao Han 국제이네비해양경제학회 2019 International Journal of e-Navigation and Maritime Vol.13 No.1

At present, ECDIS has been widely used in ship positioning, collision avoidance and navigation, and has developed into an important part of integrated navigation and automation system. However, most ECDIS software focuses too much on system function and technical level when realizing powerful navigation AIDS, neglecting the problems that the crew are most concerned about, such as realizing the human-computer interface operation quickly and simply as well as obtaining the information of human-computer interaction effectively and accurately. In order to improve the design of Electronic Chart Display and Information system(ECDIS)human-computer interface and its usability effectively, a new method for evaluating ECDIS human-computer interface is proposed. Starting from the concept and connotation of usability, five-factor personality model is used to measure the users’ personality type in reference to the essence of usability (user’s emotional experience) and calculate the basic emotional variant of users. Then, according to the users’ emotional stimulation after finishing the given ECDIS task and combining with the system Usability scale (SUS), a tool dedicated to usability testing and five dimensions of usability evaluation proposed by Jakob Nielsen, father of usability, the emotion relationship model of usability and users is established by using partial least square method ( PLS ), and the validity of the model is verified with an aim to locate the key indicators that affect the usability of the ECDIS human-computer interface, It will be more helpful to improve the usability of ECDIS human-computer interface.

Investigation on the Stratigraphic Response and Plugging Effect Induced by Press-In Open Caisson in Mucky Soil

Yongzhi Wang,Mengbo Liu,Shaoming Liao,Qiong Yi,Junzuo He,Linqi Liu,Zhiqun Gong,Kaiying Li 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.5

Press-in open caisson method, which sinks into soil with the aid of mechanical pressure without excavation, is becoming an important method of construction in extremely soft foundations. However, many engineering problems are prone to occur in the construction of caissons, such as sinking and large deformation. In this study, the stratigraphic response model, such as soil migration during the press-in process of open caisson, is focused on. Through model tests of soil and caissons, the migration patterns of each part of the soil were carried out, and the mechanism underlying the stratigraphic response pattern was investigated. The three primary conclusions are as follows: 1) The bottom edge foot of caisson produces lateral extrusion on surrounding soil in the range of 10 cm (20%H) below, resulting in lateral movement of soil. 2) two kinds of plug, i.e., “wedged” and “unwedged” plugs exist, and the driven forces for the soil migration can be divided into three stages, i.e., caused by soil plug with active arch, by soil plug with passive arch and caused by soil squeezing effect. 3) there is a significant linear correlation between the soil plug changes, the horizontal movement of the deep soil body, and the displacement of the surface uplift. The soil plugging effect and squeezing effect are interacted and enhanced with each other. This study provides a new understanding of the stratigraphic response in the construction of press-in open caissons, andguides safety control of open caissons construction.

Securing MapReduce Result Integrity via Verification-based Integrity Assurance Framework

Yongzhi Wang,Jinpeng Wei,Yucong Duan 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.6

MapReduce, a large-scale data processing paradigm, is gaining popularity. However, like other distributed computing frameworks, MapReduce suffers from the integrity assurance vulnerability: malicious workers in the MapReduce cluster could tamper with its computation result and thereby render the overall computation result inaccurate. Existing solutions are effective in defeating the malicious behavior of non-collusive workers, but are less effective in detecting collusive workers. In this paper, we propose the Verification-based Integrity Assurance Framework (VIAF). By using task replication and probabilistic result verification, VIAF can detect both non-collusive and collusive workers, even if the malicious workers dominate the environment. We have implemented VIAF on Hadoop, an open source MapReduce implementation. Our theoretical analysis and experimental result show that VIAF can achieve high job accuracy while imposing moderate performance overhead.

Translation of Poetic Spatiality

Yongzhi Liu 한국외국어대학교 통번역연구소 2019 한국외국어대학교 통번역연구소 학술대회 Vol.2019 No.07

Nonsingular Finite-time Second Order Sliding Mode Attitude Control for Reentry Vehicle

Yongzhi Sheng,Jie Geng,Xiangdong Liu,Liang Wang 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.4

This paper presents nonlinear robust flight control strategies for the reentry vehicle which is nonlinear, coupling, and includes parameter uncertainties and external disturbances. Firstly, a finitetime second order sliding mode attitude control strategy is pointed out with the introduction of a nonsingular finite-time sliding mode manifold. By the proposed controller, the attitude tracking errors are mathematically proved to converge to zero within finite time and the chattering of sliding mode controller is alleviated without any deterioration of robustness and accuracy. For further alleviation of chattering, a smooth second order sliding mode attitude control strategy is then designed based on an improved nonsingular finite-time sliding mode manifold. Finally, the proposed strategies are applied to the attitude control of X-33 RLV in the reentry phase to verify the validity and robustness of the proposed strategies.

북동아시아 페스트 발생중 두만강 유역 전염병 예방과 국제협력

Yongzhi Lee 한국재난정보학회 2017 한국재난정보학회 논문집 Vol.13 No.2

북동아시아 페스트에 관한 연구는 활발히 진행되었으나, 연변지역의 방역에 관한 내용은 거의 언급되지 않고 있다. 본 연구는 북동아시아 페스트 관련 연구자들의 연구 결과를 토대로 연변지역 지방사료들을 참고하고 당시 두만강유역(주로 현 연변지역) 폐페스트 예방조치와 영향을 파악하고자 하였다. 특히 기존 문헌을 활용하여 사망수치에 대한 검증과 비록 제한적이지만 이 지역에 있었던 국제적 협력에 대해서도 고찰하고 페스트 역병방지에 있어 성과가 있게 된 원인을 분석하고자 한다. Studies on the North East Asian Black Death have been actively conducted, but the contents of the Yanbian region have been rarely mentioned. The purpose of this study was to investigate the preventive measures and effects of the pneumonic plague in the Tumen River basin (mainly the present Yanbian region) based on the study of Yanbian region based on the research of researchers related to the North East Asia Black Death. In particular, we will use historical sources to provide a review of mortality levels and international cooperation in the region, as well as an overview the causes of the pandemic prevention efforts.

Development of Korean Communities in Northern Jiāndǎo in the 1910s and the March First Movement: Centered on the March Thirteenth Independence Demonstrations in the Lóngjǐng Region

Li Yongzhi 건국대학교 인문학연구원 2019 통일인문학 Vol.5 No.1

The peninsula-wide March First Movement in 1919 demonstrated the cohesiveness of the Korean people and served as the opening chapter to a new history; the entire peninsula was flooded with protests for independence, and shocked by their intensity, the Japanese colonial government engaged in indiscriminate suppression. The March First Movement propelled demonstrations to be held as well in Northern Jiāndǎo (“Puk-kando”), situated north of the Tumen River.Thousands of demonstrators gathered on March 13 in Lóngjǐng to read the Declaration of Independence as part of the demonstration. Although dozens of people were injured due to the suppression by the Chinese armed forces (seventeen were killed), numerous demonstrations (currently known are fifty-eight) took place throughout Northern Jiāndǎo. A frontier region, Northern Jiāndǎo was a unique cultural space wherein Koreans who crossed into this borderland formed their own communities; with active ethno-nationalist education and religious propaganda, the region served as a nexus of ethno-national and anti-Japanese consciousness. In addition, due to the frequent exchanges between the Korean peninsula and the Maritime Province, Lóngjǐng in particular served as the cradle of ethno-national independence movements.

북동아시아 페스트 발생중 두만강 유역 전염병 예방과 국제협력

Lee, Yongzhi 한국재난정보학회 2017 한국재난정보학회 논문집 Vol.13 No.2

Studies on the North East Asian Black Death have been actively conducted, but the contents of the Yanbian region have been rarely mentioned. The purpose of this study was to investigate the preventive measures and effects of the pneumonic plague in the Tumen River basin (mainly the present Yanbian region) based on the study of Yanbian region based on the research of researchers related to the North East Asia Black Death. In particular, we will use historical sources to provide a review of mortality levels and international cooperation in the region, as well as an overview the causes of the pandemic prevention efforts. 북동아시아 페스트에 관한 연구는 활발히 진행되었으나, 연변지역의 방역에 관한 내용은 거의 언급되지 않고 있다. 본 연구는 북동아시아 페스트 관련 연구자들의 연구 결과를 토대로 연변지역 지방사료들을 참고하고 당시 두만강유역(주로 현 연변지역) 폐페스트 예방조치와 영향을 파악하고자 하였다. 특히 기존 문헌을 활용하여 사망수치에 대한 검증과 비록 제한적이지만 이 지역에 있었던 국제적 협력에 대해서도 고찰하고 페스트 역병방지에 있어 성과가 있게 된 원인을 분석하고자 한다.

A Data-Driven Deep Learning Network for Massive MIMO Detection with High-order QAM

Yu, Yongzhi,Ying, Jie,Wang, Ping,Guo, Limin 한국통신학회 2023 Journal of communications and networks Vol.25 No.1

Massive multiple-input multiple-output (MIMO)can provide higher spectral efficiency and energy efficiencycompared to conventional MIMO systems. Unfortunately, asthe numbers of modulation orders and antennas increase, thecomputational complexity of conventional symbol detection algo-rithms becomes unaffordable and their performance deteriorates. However, deep learning (DL) techniques can provide flexibility,nonlinearity and computational parallelism for massive MIMOdetection to address these challenges. We propose an efficientdata-driven detection network, i.e., accelerated multiuser inter-ference cancellation network (AMIC-Net), for uplink massiveMIMO systems. Specifically, we first introduce an extrapolationfactor regarded as a learnable parameter into the multiuserinterference cancellation (MIC) algorithm for iterative sequentialdetection (ISD) detector through extrapolation technique toenhance the convergence performance. Then we unfold the aboveaccelerated iterative algorithm and adopt a sparsely connectedapproach, instead of fully connected, to obtain a relativelysimple deep neural network (DNN) structure to enhance thedetection performance through the data-driven DL approach. Furthermore, in order to accommodate communication scenarioswith higher-order modulation, a novel activation function isproposed, which is composed of multiple softsign activationfunctions with additional learnable parameters to implement amulti-segment mapping of the set of constellation points withdifferent modulations. Numerical results show that the proposedDL network can bring significant performance gain to ISDdetector with various massive antenna settings and outperformthe existing detectors with the same or lower computationalcomplexity, especially in high-order QAM modulation scenarios.