Molecular Cloning and Expression of a Novel Protease-resistant GH-36 α-Galactosidase from Rhizopus sp. F78 ACCC 30795

Ya Nan Cao,Ya Ru Wang,Hui Ying Luo,Peng Jun Shi,Kun Meng,Zhi Gang Zhou,Zhi Fang Zhang,Bin Yao 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.11

Pigment Distribution in Fresh Shoot Tissues of Four Colored-leaf Prunus Taxa

Ya Meng Li,Xiao Ling Qu,Xiao Lei Li,Ling Guo,Xiang Shen 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Identification and expression patterns of UDP-glycosyltransferase (UGT) genes from insect pest Athetis lepigone (Lepidoptera: Noctuidae)

Ya-Nan Zhang,Ji-FangMa,Lu Xu,Zhi-PingDong,Ji-Wei Xu,Meng-Ya Li,Xiu-Yun Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

UDP-glucuronosyltransferase (UGT) genes,which belong to an ancient gene family and play very important roles in all organisms, encode extracellularly secreted proteins that are involved in the transfer of glycosyl residues fromactivated nucleotide sugars to acceptor hydrophobicmolecules (aglycones). Athetis lepigone is an important polyphagous pest worldwide, and since 2011 it has become one of the major maize pests in North China. However, there have been no studies on pesticides for the effective control of this pest. In this study,we identified 23 putative UGT genes in A. lepigone by analysing previous antennal transcriptomic data. Phylogenetic analysis showed that all of the AlUGTs are distributed within 11 of 14 insect UGT sub-families. Tissue expression analysis revealed that N70% of AlUGTs were primarily expressed in adult antennae, of which three (AlUGT33AD1, AlUGT40F6 and AlUGT40L4) and four (AlUGT33B18, AlUGT33F10, AlUGT40Q3 and AlUGT41D3) displayed male-biased and female-biased expression, respectively. SomeAlUGTs, however, had higher expression levels in non-antennal tissues. Our study is the first to identify UGT genes in A. lepigone,which will help us to elucidate the diverse functions of these genes, and ultimately provide potential targets that will facilitate the development of efficient and environmentally friendly pesticides against A. lepigone.

The effect of S-functionalized and vacancies on V2C MXenes as anode materials for Na-ion and Li-ion batteries

Ya-Meng Li,Yong-Liang Guo,Zhao-Yong Jiao 한국물리학회 2020 Current Applied Physics Vol.20 No.2

The electrochemical properties of V2C and V2CT2 (T = O, S) MXenes with and without vacancy as anode materials for Na-ion and Li-ion batteries, have been studied using first-principles calculation. The present results indicate that the adsorption strength of Li-ion and Na-ion on V2CS2 are less than that of O-functionalized, together with a lower diffusion barrier. Simultaneously, V2CS2 monolayer exhibits lower open-circuit voltage (OCV) values of 0.72 and 0.49 V for Li- and Na-ion, respectively. Interestingly, the presence of atomic vanadium vacancy on V2CS2 monolayer exerts more prominent effects on enhancing adsorption strength than that of carbon vacancy for Li-ion and Na-ion, but with an exception for the diffusion of Li-ion and Na-ion on V2CS2 monolayer. The finding suggests that the V2CS2 monolayer is expected to be a potential candidate as anode material for Li-ion and Na-ion battery due to its lower open-circuit voltages and diffusion barriers.

Soluble Epoxide Hydrolase Inhibitory and Anti-inflammatory Components from the Leaves of <i>Eucommia ulmoides</i> Oliver (Duzhong)

Bai, Meng-Meng,Shi, Wei,Tian, Jun-Mian,Lei, Ming,Kim, Jang Hoon,Sun, Ya Nan,Kim, Young Ho,Gao, Jin-Ming American Chemical Society 2015 Journal of agricultural and food chemistry Vol.63 No.8

<P><I>Eucommia ulmoides</I> leaves have been used as a functional food and drink in China. The purpose of this study was to identify the bioactive constituents with soluble epoxide hydrolase (sEH) inhibitory activity and anti-inflammatory properties. Twenty-seven known compounds (<B>1</B>–<B>27</B>) were isolated from the leaves of <I>E. ulmoides</I> Oliver, and their structures were identified by NMR and ESIMS analysis; three of these, 2,5-dimethoxy-3-glucopyranosyl cinnamic alcohol (<B>11</B>), foliasalacioside E<SUB>2</SUB> (<B>26</B>), and icariside F<SUB>2</SUB> (<B>27</B>), were obtained from this plant for the first time. Compounds <B>1</B>–<B>7</B> exhibited soluble epoxide hydrolase (sEH) inhibitory activity at 100 μM; among them, quercetin (<B>1</B>) and kaempferol (<B>5</B>) displayed potential activities with IC<SUB>50</SUB> values of 22.5 ± 0.9 and 31.3 ± 2.6 μM, respectively, with noncompetitive inhibition mode. Nuclear factor kappa B (NF-κB) inhibitory activity of the isolated compounds was evaluated by the NF-κB liciferase assay in HepG2 cells. Compounds <B>1</B>, <B>9</B>, <B>20</B>, and <B>27</B> displayed potent NF-κB inhibitory effects, with IC<SUB>50</SUB> values of 15.14 ± 2.29, 15.23 ± 2.34, 16.88 ± 2.17, and 16.25 ± 2.19 μM, respectively, whereas other compounds showed weak inhibition of NF-κB transcriptional activity ranging from 17.54 to 92.6 μM. A structure–activity relationship of flavonoids <B>1</B>–<B>9</B> was also discussed. The results obtained in this work might contribute to the understanding of pharmacological activities of <I>E. ulmoides</I> leaves and further investigation on its potential application values for food and drug.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2015/jafcau.2015.63.issue-8/acs.jafc.5b00055/production/images/medium/jf-2015-00055j_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf5b00055'>ACS Electronic Supporting Info</A></P>

Molecular characterization of the sans fille gene in Antheraea pernyi: A highly conserved gene during the evolution of animals

Ya-Jie Li,Rui Mi,Nan Meng,Zhi-Xin Wen,Xue-Jun Li,Mo Chen,Yan-Qun Liu,Shu-Ying Li 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.3

Sans-fille (SNF) is the Drosophila homologue ofmammalian general splicing factors U1A and U2B″, and plays an important role in sex determination in Drosophilamelanogaster. In this study, the snf gene fromAntheraea pernyi (Lepidoptera: Saturniidae), an economically important insect, was isolated and characterized. The obtained 925 bp cDNA sequence contains an open reading frame of 669 bp encoding a polypeptide of 222 amino acids,showing 78% sequence identity to that from D. melanogaster. A database search revealed that SNF protein homologs are present in many animals, including invertebrates and vertebrates, with more than 70% amino acid sequence identities, suggesting that they were highly conserved during the evolution of animals. Phylogenetic analysis revealed that A. pernyi SNF was closely related to Bombyx mori SNF. Quantitative real-time PCR (qRT-PCR) analysis showed that the A. pernyi snf gene was transcribed during five larval developmental stages,and in six tested tissues (ovaries, testes, silk glands, fat body, integument, and hemolymph),with the most abundance determined in the gonads (ovaries or testes). Investigation of expression changes throughout embryonic development indicated that A. pernyi snfmRNAwas expressed at a lowlevel fromdays 0 to 4, and reached amaximum level at day 10, but decreased to a low level before hatching. These results suggest that the product of the snf gene may play important roles in the development of A. pernyi.

Coevolution between Human's Anticancer Activities and Functional Foods from Crop Origin Center in the World

Zeng, Ya-Wen,Du, Juan,Pu, Xiao-Ying,Yang, Jia-Zhen,Yang, Tao,Yang, Shu-Ming,Yang, Xiao-Meng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Cancer is the leading cause of death around the world. Anticancer activities from many functional food sources have been reported in years, but correlation between cancer prevalence and types of food with anticancer activities from crop origin center in the world as well as food source with human migration are unclear. Hunger from food shortage is the cause of early human evolution from Africa to Asia and later into Eurasia. The richest functional foods are found in crop origin centers, housing about 70% in the world populations. Crop origin centers have lower cancer incidence and mortality in the world, especially Central Asia, Middle East, Southwest China, India and Ethiopia. Asia and Africa with the richest anticancer crops is not only the most important evolution base of humans and origin center of anticancer functional crop, but also is the lowest mortality and incidence of cancers in the world. Cancer prevention of early human migrations was associated with functional foods from crop origin centers, especially Asia with four centers and one subcenter of crop origin, accounting for 58% of the world population. These results reveal that coevolution between human's anticancer activities associated with functional foods for crop origin centers, especially in Asia and Africa.

Chemoenzymatic Synthesis of Dual-responsive Amphiphilic Block Copolymers and Drug Release Studies

Peng Chen,Ya-Peng Li,Shu-Wei Wang,Xin-Lei Meng,Ming Zhu,Jing-Yuan Wang 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

Dual-responsive amphiphilic block copolymers were synthesized by combining enzymatic ring-opening polymerization (eROP) of ε-caprolactone (CL) and ATRP of N,N-dimethylamino-2-ethyl methacrylate (DMAEMA). The obtained block copolymers were characterized by gel permeation chromatography (GPC), 1H NMR and FTIR-IR. The critical micelle concentration (CMC) of copolymer was determined by fluorescence spectra, it can be found that with hydrophilic block (PDMAEMA) increasing, CMC value of the polymer sample increased accordingly, and the CMC value was 0.012 mg/mL, 0.025 mg/mL and 0.037 mg/ mL for PCL50-b-PDMAEMA68, PCL50-b-PDMAEMA89, PCL50-b-PDMAEMA112, PCL50-b-PDMAEMA89 was chosen as drug carrier to study in vitro release profile of anti-cancer drug (taxol). The temperature and pH dependence of the values of hydrodynamic diameter (Dh) of micelles, and self-assembly of the resulting block copolymers in water were evaluated by dynamic light scattering (DLS). The result showed that with the temperature increasing and pH decreasing, the Dh decreased. Drug-loaded nanoparticles were fabricated using paclitaxel as model. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) had been explored to study the morphology of the hollow micelles and the nanoparticles, revealing well-dispersed spheres with the average diameters both around 80 nm. In vitro release kinetics of paclitaxel from the nanoparticles was also investigated in different conditions (pH and temperature, etc.), revealing that the drug release was triggered by temperature changes upon the lower critical solution temperature (LCST) at pH 7.4, and at 37 °C by an increase of pH.

H-형태 양친매성 펜타블록 공중합체의 화학효소적 합성과 자기회합거동 평가

Peng Chen,Ya Peng Li,Can Jin Li,Xin Lei Meng,Bao Zhang,Ming Zhu,Yan-jing Liu,Jing Yuan Wang 한국고분자학회 2013 폴리머 Vol.37 No.3

H-shaped amphiphilic pentablock copolymers (PSt)2-b-PCL-b-PEO-b-PCL-b-(PSt)2 was synthesized via chemoenzymatic method by combining enzyme-catalyzed ring-opening polymerization (eROP) of ε-caprolactone (ε-CL) and atom transfer radical polymerization (ATRP) of styrene. By this process, we obtained copolymers with controlled molecular weight and low polydispersity. The structure and composition of the obtained copolymers were characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC) and infrared spectroscopy analysis (IR). The crystallization behavior of the copolymers was analyzed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). The crystallization behavior of the H-shaped block copolymers demonstrated a PCL dominate crystallization. The self-assembly behavior of the copolymers was investigated in aqueous media. The hydrodynamic diameters of the copolymer micelles in aqueous solution were measured by dynamic light scattering (DLS). The morphology of the copolymer micelles was observed by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hydrodynamic diameters of spherical micelles declined gradually with the increase of the hydrophobic chain lengths of the copolymers. The critical micelle concentration (CMC) values were determined from fluorescence emission, and it was found that the CMCs decreased with an increase of PSt hydrophobic block lengths.

Transcriptome analysis of differentially expressed genes in rabbits’ ovaries by digital gene-expression profiling

Tao Huang,Ya‑dong Wang,Ming‑ming Xue,Xue Feng,Cai‑Xia Sun,An‑si Wang,Shu‑yu Xie,Meng Zhang,Gui‑Rong Sun,Ming Li 한국유전학회 2018 Genes & Genomics Vol.40 No.7

Reproduction is a complex physiological process that is regulated by multiple genes and pathways. Compared with studies of common livestock, fewer studies of genes related to the fertility of rabbits (Oryctolagus cuniculus) have been reported, and the molecular mechanism of their high productivity is still poorly understood. To identify candidate genes associated with development and prolificacy in rabbits, we analyzed gene expression differences among the ovaries of mature Californian rabbit (LC), and mature (HH) and immature Harbin white rabbit (IH) using digital gene expression technology. We detected 885 and 321 genes that were significantly differentially expressed in comparisons between HH/IH and HH/LC, respectively. The functions of the differentially expressed genes (DEGs) were determined by GO classification and KEGG pathway analysis. The results suggest that most of the DEGs between the mature and immature developmental stages were predominantly associated with DNA replication, cell cycle, and progesterone-mediated oocyte maturation, and most were up-regulated in the IH group compared with the HH group. The DEGs involved in disparate fecundities between HH and LC were associated with reproduction, fructose and mannose metabolism, steroid hormone biosynthesis, and pyruvate metabolism. Our results will contribute to a better understanding of changes in the regulatory network in ovary at different developmental stages and in different fertility of rabbit.