실험적 치아이동시 glycosaminoglycan의 발현에 관한 연구

이경환,이종진,강경화,김은철,김상철 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.4

치아이동시 골대사에 있어 glycosaminoglycan의 역할에 대해 알아보고자 glycosaminoglycan의 주요 구성 성분인 chondroitin 4-sulfate(CH-4S)의 치주조직 내에서의 면역반응 정도 및 분포 양상을 백서 치아의 실험적 이동 과정에서 면역조직화학적으로 관찰하였다. 또한 사람 치주인대세포를 배양하여 여러 종류의 cytokine을 투여한 후 CH-4S의 발현 양상의 변화를 Western blot analysis를 통해 확인하여 다음과 같은 결과를 얻었다. 1.치아이동에 반응하는 치수, 치주인대, 골모세포, 파골세포, 골세포 부위에서의 CH-4S 발현이 대조군보다 많았으나 상아질, 백악질에서의 CH-4S의 발현은 견인력 적용 기간에 관계없이 대조군과 큰 차이가 없었다. 2.치수에서 CH-4S의 발현은 교정력을 가한 1일째에 크게 증가하였다가 7일째부터 감소되었으며 14일째에는 대조군과 차이가 없었다. 3.치주인대에서 CH-4S의 발현은 주로 치조골 면을 따라 견인측에서 나타났는데 교정력을 가한 1일째에 가장 많은 발현을 보인 후 4일째부터 감소하기 시작하였다. 4.골모세포와 파골세포 및 골세포에서 CH-4S의 발현은 4일째에 가장 많은 발현을 보였고 7일째 이후에는 크게 감소 하였다. 5.치주인대세포에 PDGF-BB를 투여한 경우 3일째에 가장 많은 CH-4S의 발현을 보였다. 6.치주인대세포에 TNF-α 처리 시 배양 1일째에 CH-4S의 발현 감소를 보였다. 7.치주인대세포에 PDGF-BB와 TGF-β를 혼합 투여한 경우가 PDGF-BB 및 TGF-β를 단독 투여한 경우 보다 배양 3일째에 CH-4S의 발현이 많았고 LPS나 TNF-α 투여군은 유사한 발현 감소를 보였다. 이상과 같이 교정적 치아이동시 시기 및 부위에 따라 glycosaminoglycan의 발현이 차이를 보이며, 치주인대세포에서도 cytokine의 자극에 따라 glycosaminoglycan의 발현이 변화하는 것으로 보아, glycosaminoglycan이 골대사에 있어 중요한 조절 인자로서의 역할을 하는 것으로 여겨진다. (주요 단어 : 치아이동, 치주인대세포, 골대사, glycosaminoglycan, chondroitin (CH) 4-sulfate) The purpose of this study was to evaluate 1) in vivo, the expression of chondroitin 4-sulfate (CH-4S), a structural element of glycosaminoglycans(GAGs), in periodontal tissue during the experimental movement of rat incisors, by labelled streptavidine biotin immunohistochemical staining for CH-4S, 2) In vitro, the expression of CH-4S in cultured human periodontal ligament(PDL) cells supplemented with 10ng/㎖ of TGF-β1, 20ng/㎕ of PDGF-BB, 1ng/ml TNF-α, or 1㎍/ml LPS by western blot analysis. The results of this study were as follows ; 1.The expression of CH-4S was stronger in pulp, PDL, osteoblasts, osteoclasts and osteocytes in experimental group than in control group, but was rare in dentin, and cementum of experimental groups, regardless of the duration of force application, which was not different from that of control group. 2.In experimental group, the expression of CH-4S in pulp began to increase at 1 day after force application and got to the highest degree at 7 days. After 14 days, the expression in CH-4S immunoreactivity was decreased, and became similar to that of control group at 28 days. 3.The expression of CH-4S in PDL was noted in adjacent to alveolar bone. PDL showed higher intensity of immunolabelling after 1 day of orthodontic tooth movement. And the expression was more stronger in the tension side than that of pressure side of PDL at 1 day, but more stronger in the pressure side than that of tension side of PDL at 4 days. After 7 days, a decrease in CH-4S expression was observed. 4.The expression of CH-4S in alveolar bone got to the highest degree at 4 days, and At 7 days, a decrease in CH-4S expression was observed. 5.PDGF-BB notably raised the expression of CH-4S in the PDL cells at 3 days of cultivation 6.The expression of CH-4S of PDL cells was decreased with the application of TNF-α at 1 day. 7.Admixture of TGF-β1 and PDGF-BB got more expression of CH-4S in PDL as compared to only TGF-β1 or PDGF-BB. A similar decrease of the expression of CH-4S was observed in the case of application of LPS or TNF-α. ※ Key words : Tooth movement, Periodontal ligament cell, Bone metabolism. Glycosaminoglycans,Chondroitin 4-sulfate

골절단을 이용한 급속 치아이동 후 치수 및 치주조직 변화

강경화,김은철,이선경,임채웅,마쓰다 기꾸,태기출,김상철 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.2

본 연구는 골절단을 이용한 치아-치조골 분절의 급속 치아이동 후 치수, 치주인대 및 치조골의 변화를 유성견에서 평가하고자 하였다. 하악 제4소구치의 근심, 원심, 치근단 부위에서 피질골을 절단하여 치아-골 분절을 형성하고 하악 제3소구치를 발거하면서 협측, 설측 부위의 피질골을 삭제하였다. 1주 휴지기 부여에 따라 휴지기군과 비휴지기군으로 나누어 치아의존형 견인장치로 6일 동안 견인하고 강화기를 거친 후 0주, 1주, 2주, 4주, 6주, 8주에 희생시켜 치수, 치주인대 및 치조골의 조직 변화를 임상적, 방사선적, 조직학적 및 면역화학적으로 평가하여 다음과 같은 결과를 얻었다. 1. 치아이동 양과 강화기 동안의 조직 치유 양상에서 휴지기 유무에 따른 차이는 없었다. 2. 견인측에서 골형성은 강화기 8주까지 계속되었는데 강화기 1-2주에 가장 활발하였으며 6-8주간의 변화는 적었다. 3. 비휴지기군의 강화기 1주에서 치조골 흡수 및 파골세포 출현, 염증세포 침윤이 가장 많았으며, 특징적으로 파상아세포가 압박측의 치주인대와 치수 내에 나타났다. 4. TGF-β는 치조골의 골기질 및 골모세포, 파골세포, 치수 내 파상아세포에서 강양성 발현을 보인 반면 치수, 백악모세포, 무세포성 백악질에서 경미한 양성 발현을 나타내는 부위 특이성이 있었다. 5. TGF-β는 견인측 치주인대의 치조골에 인접한 혈관 및 치주인대세포, 골모세포에서 강화기 초기 1-2주에 주로 발현되었으며 6주 이후에는 발현이 크게 감소하였다. The movement of tooth-bone segments by osteotomy can simultaneously shift tooth and surrounding alveolar bone in a relatively short period. The purpose of this study was to evaluate the tissue changes in pulp, periodontal ligament, and alveolar bone in rapid tooth bone movement with osteotomy. The mandibular 3rd premolar of a dog was extracted and cortical bones of the buccal and lingual area were eliminated, and then cortical bones around the mesial and distal area of root, and below the root apex of the mandibular 4th premolar were osteotomized. After a one week latency period, a tooth-borne distraction device was activated for 6 days. And pulp, periodontal ligament and alveolar bone were evaluated clinically, radiologically, histologically and immunohistochemically at 0, 1, 2, 4, 6, 8 weeks of the consolidation period and conclusions were reached as follows. 1. Latency period didn't affect total amount of tooth movement and healing process of tissue during consolidation period. 2. Bone formation continued through 8 weeks of consolidation in distracted side, with a high peak at 1-2 weeks, and the lowest at 6-8 weeks of consolidation. 3. At 1 week of consolidation, alveolar bone resorption, osteoclast appearance and inflammatory cell infiltration were the most active, and dentinoclasts characteristically appeared on the pulp and pressure side of the periodontal ligament. 4. The expression of TGF-β was area-specific, as it was strong-positive at bone matrix, osteoblast, osteoclast of alveolar bone, and dentinoclast inside pulp, but weak in pulp, cementoblast and acellular cementum. 5. The expression of TGF-β was generally observed at the initial 1-2 weeks of consolidation at vessels, periodontal ligament cells, and osteoblast near alveolar bone on the distraction side of the periodontal ligament, and was significantly decreased after 6 weeks of consolidation.

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.

한국인 알코올의존환자에서의 제1형 부신피질자극 호르몬 분비 촉진 호르몬수용체의 유전자 다형성에 관한 연구

김철민,김성곤,김지훈,김현경,김미경,유정현,민은정 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

스트레스 반응에 중요한 역할을 하고 있는 CRHR1 유전자 SNP와 알코올 의존과의 연관성에 관한 선행 연구들이 있다. 이에 본 연구는 백인을 대상으로 조사했던 기존연구와는 달리 한국인 남녀를 대상으로 하여 알코올 의존군과 정상 대조군의 CRHR1 유전자형 빈도 및 대립유전자형 빈도를 비교하였다. 본 연구의 연구대상자들은 알코올 의존군 268명(남자 218명, 여자 50명)과 정상 대조군 137명(남자 78명, 여자 59명)이었다. CRHR1 유전자 SNP를 SNP database와 선행연구를 근거로 선정한 후, 연구 대상자들의 이러한 SNP 유전자형을 PCRRFLP방법으로 조사하였다. 그 결과, 한국인의 CRHR1 유전자 SNPs로 7개(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887)가 분석되었다. 이중 rs28364027의 A 대립 유전자의 빈도는 남녀 각각에서 알코올 의존군이 대조군보다 유의하게 높았다. 그리고 rs28364027의 AA 유전자형 및 A 대립 유전자와 rs878886의 CC유전자형 및 C 대립유전자의 빈도는 알코올 의존군내에서 여성이 남성보다 유의하게 높았다. 이러한 결과를 종합해 보면 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인의 중요한 요인일 가능성을 제시하고 있다. 또 이러한 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인에서 남녀간 차이를 설명할 수 있을 가능성도 시사하고 있다. Objectives:There were several preceding studies investigating the association between alcohol dependence and corticotropine releasing hormone receptor1(CRHR1) gene SNPs that play an important role in response to stress. The frequencies of CRHR1 genotypes and alleles were compared between alcohol-dependent patients and normal control subjects. Methods:The subjects were 268 alcohol-dependent patients(218 males, 50 females) and 137 normal controls(78 male, 59 female). CRHR1 gene SNPs were investigated according to the SNP database and the results from previous studies, and their genotypes were analyzed by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP). Results:Seven CRHR1 gene SNPs(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887) were found in Korean subjects. The frequency of rs28364027 A allele was significantly higher in alcohol-dependent patients than normal controls in both genders. In alcohol-dependent patients, the frequencies of AA genotype and A allele of rs28364027, and of CC genotype and C allele of rs878886 were higher in female than male. Conclusion:These results suggest that CRHR1 gene SNP is one of the important genetic factors in the etiology of alcohol dependence. And also it is supposed that the different frequency of SNP genotype could explain stress-related gender difference in the genetic etiology of alcohol dependence.

개에서 발생한 이혈종의 임상적 관리

이우철,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 14-year-old male pomeranian was present with swollen left ear. The swelling occurred from base to apex of pinna. Otitis externa persisted for two years. Hematological and serum biochemistry values were not remarkable. The surgery treatment was the most appropriate method among indwelling drain, surgical, and steroid treatment in the aural hematoma because the dog was very sensitive to touching pinna and the size of aural hematoma. Longitudinal incision was applied after general anesthesia. Cefovecin was injected to prevent secondary infection. The outcome of surgery was good. There was no evidence of recurrence for one year.

합천 박실늪의 교란된 습지에서 식생의 회복 과정

김철수,오경환 경상대학교 환경보전연구소 1999 環境保全硏究所報 Vol.7 No.-

버들류 (Salix spp.)가 우점한 경남 합천군 박실지의 연안대에서 1997년과 1998년에 각각 3월부터 8월까지 인위적인 교란전·후의 식생구조와 토양환경을 조사하여 식생의 회복 과정을 밝혔다. 수생 및 습생 관속식물상을 비교한 결과, 교란 전에는 41과 91속 105종, 20변종으로 125종류, 교란 후에는 50과 119속 141종 24변종, 1품종으로 166종류가 각각 출현하여 종수가 증가하였다. 교란 전·후에 출현한 관속식물을 Swan (1970)의 기준에 따라 증가종 (increaser), 감소종 (decreaser), 중립종 (neutral), 침입종 (invader), 후퇴종 (retreater) 등의 5개 범주로 구분한 결과 전체 207종류 가운데 82종(40%)이 새로 나타난 침입종이고, 41종(20%)은 사라진 후퇴종으로서 모두 123종 (60%)의 종조성이 변화되어 식생 변화가 크게 나타남을 알 수 있었다. 교란 전·후의 생활형 조성은 Th-D_1-R_6-e형으로 동일하였고, 유사도지수는 CCs= 0.81 및 C=0.89이었으며, 종다양서지수는 2.45 및 2.52로서 약간 증가하였다. 교란 전·후의 토양 성분을 분석한 결과 pH는 5.14 및 6.18, 전기전도도 (㎛cm^-1)는 99.64 및 70.61, 유기물량 (%)은 2.17 및 1.46, 총질소량 (%)은 0.06 및 0.04, 유효인량 (mg/100g)은 0.15 및 0.08등으로서, 교란 후에 pH는 증가하였으나, 전기전도도, 유기물량, 총질소량 및 유효인량은 감소하였다. Vegetation structure and soil properties were investigated before and after artificial disturbance of willow forest to reveal the vegetative regeneration at the Parksil-nup wetland in Hapchoˇn-gun, Kyoˇngsangnam-do, Korea from March to August in 1997 and 1998. The flora of the undisturbed year was composed of 41 families, 91 genera, 105 species and 20 varieties or 125 taxa of the vascular hydrophytes and hygrophytes, and that of the disturbed year was 50 families, 119 genera, 141 species, 24 varieties and 1 form or 166 taxa of vascular plants, and total 207 taxa. Among 207 taxa, 82 taxa (40%) was classified as the invaders and 41 taxa (20%) as the retreaters by the Swan (1970). The biological spectra of the undisturbed and the disturbed year were same as Th-D_₁R_5-e. Similarity indices between the disturbed and the undisturbed years were CCs=0.81 and C=0.89, and species diversity indices (H') were 2.52 in the disturbed year and 2.45 in the undisturbed year. Sediment properties of the undisturbed and the disturbed year were pH 5.14 and 6.18, conductivity 99.64 and 70.61㎛ho㎝^-1, organic matter 2.17 and 1.46%, total nitrogen 0.06 and 0.04%, and available phosphrous 0.15 and 0.08 ㎎/100g, respectively. Conductivity, organic matter, total nitrogen, and available phosphorus decreased after the artificial disturbance.

백서 두개골에 형성된 골 결손부 크기에 따른 골 치유과정에 대한 조직학적 연구

윤도철,김경욱,임창준,김종여 단국대학교 치의학연구소 1994 단국대치의학연구소논문집 Vol.6 No.1

태양열 이용 냉난방 공조시스템 중 재생기의 하계 재생 능력에 관한 연구

김보철,최광환,금종수,김경철,김종렬 동의공업대학 1998 論文集 Vol.24 No.1

The humidity control is conducted not only for industrial applications but also for domestic ones. High humidity is a factor causing discomfort, thus the importance of using a LiCl(Lithium Chloride) solution has been recognized in recent years in connection with the high quality of goods to be manufactured. as well as cleanliness in a special environment against air pollution and environmental protection. This paper will examine the solar energy regenerator as a part of an open cycle solar energy absorption system for dehumidifying and drying purposes using LiCl solution. An outdoor experiment was done to find out how much could be regenerated in summer. It was found that regeneration rate was about 10kg/day at best in August, and about 5kg/day in September.

酸洗廢棄 酸化鐵의 水素還元에 관한 硏究

蘇京洙,李大喆 全北大學校 1983 論文集 Vol.25 No.-

In the manufacturing process for obtaining reduced iron from iron oxide waste, the production of reduced iron is strictly controlled by various reduction conditions of hydrogen gas. Therefore, in order to increase the reduction rate, fundamental knowledges on the effects of various factors and their mechanism has reviewed. In this study, time temperature porosity, flow rate of hydrogen gas and various particle size dependence of reduction rate for a iron oxide waste were studied. The results obtained in this study are as follows: 1. Higher reduction rates were obtained for the finer particle sizes and the larger porosity of pellet. 2. The reduction rate increased with the increase with the increase if the temperature. The optimum temperature was 750℃ or higher. 3. With a prolonged period of the reduction time, much higher reduction rate was obtained. The optimum period was 30 minutes or longer. 4. As the hydrogen gas flow rate was increased, the reduction rate was increased. The optimum flow rate was 300ml/minute.

Maximum Ratio Transmission 기법의 MIMO 다중경로 페이딩 채널로의 확장

이경규,이원철,신요안 崇實大學校 生産技術硏究所 2001 論文集 Vol.31 No.-

To effectively improve capacity on fading channels in the third generation and beyond mobile communication systems that require high quality and capacity, spatial diversity schemes utilizing MIMO (Multi-Input Multi-Output) concepts have been identified as an efficient way of improving the capacity without expanding the bandwidth. Among various MIMO spatial diversity schemes, MRT (Maximum Ratio Transmission) which maximizes signal-to-noise ratio at the reception has been recently proposed. In this paper, we extend the conventional MRT scheme which is restricted to flat fading channels to MIMO multipath fading channels, and analyze the bit error rate performance.