연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

소규모 하수처리를 위한 파일럿 규모 이중슬러지 KNR^® (Kwon's nutrient removal) 시스템의 영얌염류 제거성능 평가

안진영,권중천,김윤학,정유훈,김두언,유선호,김병우,An, Jin-Young,Kwon, Joong-Chun,Kim, Yun-Hak,Jeng, Yoo-Hoon,Kim, Doo-Eon,Ryu, Sun-Ho,Kim, Byung-Woo 한국청정기술학회 2006 청정기술 Vol.12 No.2

본 연구에서 소규모 하수고도처리를 위한 이중슬러지(Dual sludge) $KNR^{(R)}$ (Kwon's nutrient removal) 시스템이 개발되었다. $KNR^{(R)}$ 시스템은 부유성장식 탈질미생물과 부착성장식 질산화미생물을 분리시킨 이중슬러지 공정으로 최초침전조, 혐기조, 무산소조, 농축조의 복합기능을 수행하는 UMBR (Upflow multi-layer bioreactor)과 펠렛형 담체가 충진된 호기성 담체조로 구성되어 있다. 소규모 하수처리시 본 개발공정의 안정성과 처리성능을 평가하기 위해 처리용량 $50m^3/d$ 규모의 파일럿 플랜트를 고도처리 공정으로 개선공사 중인 처리용량 $50m^3/d$ 규모의 실제 소규모 마을하수처리장에 적용하였다. UMBR과 담체조의 체류시간은 각각 4.7 h와 7.2h이었으며, 반응조 수온은 $18.1{\sim}28.1^{\circ}C$이었다. 유입 하수량과 유입수의 BOD/N의 변동폭이 컸음에도 불구하고 파일럿 플랜트는 안정된 처리성능을 보였다. 전체 실험기간 중 처리수의 $COD_{cr}$, $COD_{Mn}$, $BOD_5$, TN, TP의 평균 농도는 11.0 mg/L, 8.8 mg/L, 4.2 mg/L, 3.5 mg/L, 9.8 mg/L, 0.87/0.17 mg/L (poly aluminium chloride(PAC) 투입/미투입)이었으며, 제거율을 각각 95.3%, 87.6%, 96.3%, 96.5%, 68.2%, 55.4/90.3% 이었다. 잉여슬러지 발생량은 $A_2O$와 Bardenpho 등과 같은 단일슬러지를 이용하는 고도처리공정과 비교시 약 1.9~3.8배 낮은 $0.026kg-DS/m^3$ and 0.220 kg-DS/kg-BOD로 나타났다. A simple dual sludge process, called as $KNR^{(R)}$ (Kwon's Nutrient Removal) system, was developed for small sewage treatment. It is a hybrid system that consists of an UMBR (Upflow multi-layer bioreactor) as anaerobic and anoxic reactor with suspended denitrifier and a post aerobic biofilm reactor, filled with pellet-like media, with attached nitrifier. To evaluate the stability and performance of this system for small sewage treatment, the pilot-scale $KNR^{(R)}$ plant with a treatment capacity of $50m^3/d$ was practically applied to the actual sewage treatment plant, which was under retrofit construction during pilot plant operation, with a capacity of $50m^3/d$ in a small rural community. The HRTs of a UMBR and a post aerobic biofilm reactor were about 4.7 h and 7.2 h, respectively. The temperature in the reactor varied from $18.1^{\circ}C$ to $28.1^{\circ}C$. The pilot plant showed stable performance even though the pilot plant had been the severe fluctuation of influent flow rate and BOD/N ratio. During a whole period of this study, average concentrations of $COD_{cr}$, $COD_{Mn}$, $BOD_5$, TN, and TP in the final effluent obtained from this system were 11.0 mg/L, 8.8 mg/L, 4.2 mg/L, 3.5 mg/L, 9.8 mg/L, and 0.87/0.17 mg/L (with/without poly aluminium chloride(PAC)), which corresponded to a removal efficiency of 95.3%, 87.6%, 96.3%, 96.5%, 68.2%, and 55.4/90.3%, respectively. Excess sludge production rates were $0.026kg-DS/m^3$-sewage and 0.220 kg-DS/kg-BOD lower 1.9 to 3.8 times than those in activated sludge based system such as $A_2O$ and Bardenpho.

구치의 경사도와 회전정도가 발치공간 폐쇄시 치근막의 응력분포와 치아의 초기이동에 미치는 영향에 대한 3차원 유한요소법적 연구 : Three dimensional finite elements study

권대우,손우성,양훈철 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.5

본 연구는 상악 제1대구치에 발치공간 폐쇄를 위한 근심력 적용시, 대구치의 근원심 경사도와 회전정도의 변화가 치근막의 응력분포와 치근막 내에서의 초기 치아이동에 어떤 변화를 야기하는지를 살펴보기 위하여 시행되었다. 상악 제1대구치와 치근막의 3차원 유한요소모델을 제작하여 20˚의 근심경사와 원심경사를 설정하고. 20˚의 근심회전과 원심회전을 설정하였다. 각각의 상황에서 치체이동을 유도하는 근심력을 가하기 위해 협면에 rigid element를 부착하고 200g의 근심력과 9:1의 countertipping 모멘트. 5:1의 counterrotation 모멘트를 부여하였다. 각각의 응력분포 및 초기이동 양상을 비교하여 다음과 같은 결론을 얻었다. 상악 제1대구치의 경사도 변화나 회전 변화를 통해 근심력 적용시 구치의 근심이동에 대한 저항의 증가를 얻을 수 없었다. 20˚의 근심경사와 20˚의 원심회전이 매우 유사한 응력분포 및 변위를 나타내었다. 20˚의 원심경사와 20˚의 근심회전도 마찬가지로 유사성을 보였다. 20˚ 근심경사 및 20˚ 원심회전된 경우 치근이 협측 아동하고 치관이 설측 이동하는 관상면 상에서의 회전경향이 발생하였다. 20˚ 원심경사 및 20˚ 근심회전된 경우 치근이 설측 이동하고, 치관이 협측 이동하는 관상면 상에서의 회전경향이 발생하였다. 경사되거나 회전된 치아에 근심력을 가해야 할 경우, 관상면상에서의 회전을 방지하기 위한 부가적인 토크 조절이 필요하다. The purpose of this study was to evaluate the stress distributions at the periodontal ligament (PDL) and displacements of the maxillary first molar when mesially directed force was applied under various molar angulations and rotations. A three dimensional finite element model of the maxillary first molar and its periodontal ligament was made. Upright position, mesially angulated position by 20˚ and distally angulated position of the same degree were simulated to investigate the effect of molar angulation. An anteriorly directed force of 200g, countertipping moment of 1,800gm-mm (9:1 moment/force ratio) and counterrotation moment of 1,000gm-mm (5:1 moment/force ratio) were applied In each situation. To evaluate the effect of molar rotation on the stress distribution, mesial-in rotation by 20˚ and the same amount of distal-in rotation were simulated. The same force and moments were applied in each situation. The results were as follows: In all situations, there was no significant difference in mesially directed tooth displacement. Also, any differences in stress distributions could not be found, in other words, there were no different mesial movements. Stress distributions and tooth displacement of the 20˚ mesially angulated situation were very similar with those of the 20° distal-in rotated situation. The same phenomenon was obserned between the 20° distally angulated situation and 20˚ mesial-In rotated situation. When the tooth was mesially angulated, or distal-in rotated, mesially directed force made the tooth rotate In the coronal plane, with its roots moving buccally, and Its crown moving lingually. When the tooth was distally angulated, or mesial-in rotated, mesially directed force made the tooth rotate in the coronal plane, with its roots moving lingually, and its crown moving buccally. When force Is applied to an angulated or rotated molar, the orthodontist should understand that additional torque control is needed to prevent unwanted tooth rotation in the coronal plane.

성과 에이즈에 관한 보건교육 방법별 효과 측정

권관우,이경무,김훈수,김정순,정경균 韓國保健敎育.健康增進學會 2000 보건교육건강증진학회지 Vol.17 No.2

The objective of this study is to find the most effective educational method on Sex and AIDS/HIV among young people in Korea. The number of people with HIV amounts to 1,224 as of Sep. 2000, and is increasing continuously, especially among young people(National Institute of Health, 2000). In setting up the policy of health education on Sex and AIDS, the criteria should be the effectiveness. By two-stage cluster sampling, 4,713 students were selected from among the 19,093 students from 44 middle and high schools and divided into three groups and taken the education with three different methods(〈lecture with slides show〉, 〈lecture without any material〉 and 〈lecture through broadcasting system〉) by the same lecturer with the same contents for about 50 minutes. To compare the effects of education by methods, the tests on the AIDS knowledge, AIDS attitude on AIDS and Sexuality attitudes with self-administered questionnaire were implemented three times, that is, 'before', 'right after' and '2 weeks later' of the education. As to the decrees of change of AIDS knowledge, AIDS attitudes, Sexuality attitudes and subjective evaluation of the education, 〈lecture with slides show〉 was the most effective, 〈lecture without any material〉 was the second and 〈lecture through broadcasting system〉 was the least effective, respectively. In the cost-effectiveness analysis using the results of this study, 〈lecture with slides show〉 turned out to be the most effective, and 〈lecture through broadcasting system〉 was the second and 〈lecture without any material〉 was least effective. From these results, it is suggested that 〈lecture with slides show〉 is more effective health educational methods on Sex and AIDS than 〈lecture without any material〉 or 〈lecture through broadcasting system〉.

근긴장성 이영양증 환자 1례에 대한 보고

권용욱,조종훈,정필현,강석 東國大學校醫學硏究所 1997 東國醫學 Vol.4 No.-

근긴장성 이영양증은 1909년 Steinert에 의해 처음으로 보고된 질환으로 상염색체 우성유전으로 발현되며 근긴장증 이외에 전신증상이 함께 나타나는데, 전두두 대머리, 긴 얼굴, 안검하수, 역전된 미소모양의 입술등이 보이고 식도, 대장, 자궁 등의 평활근도 침범하여 연하곤란, 구음장애, 변비, 분만장애 등을 일으킬 수 있다. 또한 심장비대와 심장전도의 이상을 동반하는 경우 이로 인한 부정맥이 주요 사망원인이 될 수 있다. 이러한 특징적인 임상증상 외에 근전도 검사상 일명 dive bomber sound 또는 motorcycle sound를 보이는 운동단위전위가 특징적으로 관찰된다. 본 교실에서는 좌측 근위약을 호소하고 원위부보다 근위부 근력이 더 심하게 약화되어 있는 근긴장성 이 영양증 환자 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Myotonic dystrophy, first described by Steinert in 1909, is an autosomal dominant multisystem diease. Most patients note the insidious onset of weakness in the limb muscles or difficulty relaxing muscles after a strong contraction. The evidence of Multisystem involvement includes early frontal balding, less than average intelligence, posterior subcapsular cataracts, abnormal cardiac conduction and testicular tubular cell atrophy. In addition, there is wasting of the facial musculature, including the temporalis, masseter, and stemocleidomastoid muscles, which produces a characteristic long face with ptosis, hollow temples, and a hanging jaw. Electromyography is extremely helpful in making the diagnosis. The presence of myotonic discharges in affected muscles is characteristic finding in electromygraphy. Both the amplitude of the potentials and the frequency of the discharges wax and wane. The sound of the discharges is quite characteristic and in their recognition which is similar to a dive bomber sound or motorcycle sound. We recently experienced a case of myotonic dystrophy who complained unusual symptoms such as the left side weakness, proximal muscle weakness rather than distal muscle weakness. So we report this case with review of the literatures.

접종능이 우수하고 페이딩 속도가 느린 구상 흑연 주철용 접종제 개발

권해욱,안종헌,최훈영 嶺南大學校 工業技術硏究所 2003 工業技術硏究所論文集 Vol.31 No.1

The performance of inoculant for ductile cast iron was investigated. The ability and fading rate of inoculant were evaluated through thermal analysis during holding the cast iron melt after the nodularizer and inoculant had been added. The effect of the addition of minor elements was also examined.

다공질 Si의 열적 안정성 연구

권영해,조훈영,홍치유 동국대학교 자연과학연구원 1995 자연과학연구 논문집 Vol.15 No.-

The oxidation and pressure effects of electrochemically etched porous Si have been investigated by photoluminescence(PL) and Raman spectroscopy. The porous Si sample was etched with a current density of 50mA/㎠ for 20 min, and shows a PL signal at 710 nm and a Raman peak at 517 cm. Rapid-thermal-annealing of the as-etched sample at 400℃ shows a rapid drop in the PL intensity. On the other hand, the intensity of the rapid-thermal-oxidized sample remains constant in the temperature range from 600℃ to 800℃. The Raman signal of the oxidized sample also showed thermal stability. The reverse leakage current of the oxidized porous Si was reduced to 100 times in comparision to that of as-grown porous Si. The PL peak position of the oxidized porous Si shows the shifts to 705 nm and the Raman peak shifts to a higher energy, closer that of the bulk polycrystalline Si. This can be explained in terms of the stress on the Si columnar structure. The initial tensile stress induced by the etching process is reduced by the compressive stres caused by the formation of oxides (SiO₂) in the gaps. It is supposed that this can cause the blue shift in the PL spectrum and the recovery of the Raman peak position.

소형 연 X-선 현미경

권영만,김한경,강성훈,김경우,윤권하 원광대학교 대학원 2008 論文集 Vol.41 No.-

A Soft x-ray microscope operates in the 'water window' wavelength region (2.3-4.4 nm), where natural contrast between carbon (protein) and oxygen (water) allows to image a biological cell. The compact soft x-ray microscope operates at λ = 2.88 nm (430 eV) and consist of liquid-nitrogen-jet-target laser-plasma x-rays source, a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate, and a thinned back-illuminated charge coupled device with 1,024 × 1,024 pixels. The Liquid-nitrogen-jet-target laser-plasma source was a high brightness of 1.4×10^(12) photons/pulse·sr·line. The tandem ellipsoidal condenser mirror has 15% reflectivity. The photon flux arrived at the sample is 1.29×10^(5) photons/μ㎡ The micro zone plate was used for an objective optic, and had outermost zone width of 30 nm, zone number of 667, and diameter of 80㎛, and the focal length was 835㎛. On performance test for the compact soft x-ray microscope, the 'Siemens Star' image was obtained with a 60-nm-resolution at 380 magnification and 3 minutes exposure. I also obtained images of biological samples, such as diatom and red blood cell of a mouse with 5-10 minutes exposure.

신경회로망 제어기를 이용한 PID 파라미터 추정에 관한 연구

權重東,裵銀敬,金恩基,全基英,李承桓,吳鳳煥,李勳九,金容珠,韓慶熙 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-

In this paper, supposed to solve these problem to PID parameters controller algorithm using ANN. In the proposed algorithm, the parameters of the controller were adjusted to reduce by on-line system the error of the speed of IM. In this process, EBPA NN was constituted to an output error value of an IM and conspired an input and output. The performance of the self-tuning controller is compared with that of the PH) controller tuned by conventional method (Ziehler-Nichols). The effectiveness of the proposed control method is verified thought the Matlab Simulink and experimental results.

Water glass로부터 ZSM-5의 합성

우훈희,홍지숙,서정권,이관영,이정민 한국공업화학회 2002 응용화학 Vol.6 No.2

In order to seek optimum conditions for ZSM-5 formation in the absence of organic template,the effects of both starting composition and water glass as silica source were examined. Water glass was most inexpensive among silica sources, but this was difficulty in reactant control because of contained much alkali ingredient, Considering environmental problem and economic performance,chose alkali drawing-out method to use methanol newly to remove alkali ingredient of waterglass. Characterization of ZSM-5 was carried out by means of XRD, SEM.