대장암환자에서 자연살해세포의 변화

김광호,심강섭,박응범,강은숙,홍기숙 대한대장항문학회 1998 Annals of Coloproctolgy Vol.14 No.3

RNase A 와 B 에 대한 Mouse Immunoglobulin의 생성 및 분리조건

전숙영,이광표,김하형 중앙대학교 약학연구소 1997 약학 논총 Vol.11 No.-

Ribonuclease A, B (RNase A, B) are the enzymes catalyse he hydrolysis of 3',5'-phosphodiester linkages of ribonucleic acids. RNase B(M.W. 15.5KDa) differs from RNase A(M.W. 13.6KDa) in that RNase B has five glycoforms consisting of Man_9GlcNAc_2, to Man_9GlcNAc_2 at the single glycosylation site (Asn-34). It has not been fully studied that the difference of glycosylation between RNase A and RNase B would make difference in production of polyclonal antibody when it was used as antigen. In the present study, male Balb/c mice aged 6-7weeks were used, and three mice per group were immunized intraperitoneally with 0.2ml of emulsion mixture containing 100ug RNase A or B in 0.01ml of PBS and 0.18ml of Freund's adjuvant, and immunized with PBS as a control. To identify the production of polyclonal antibody, total protein amount was detected by use of UV- spectrophotometer and tested 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In order to know the affinity of polyclonal antibody to RNase A, B and to measure the concentration of polyclonal antibody. ELISA method had been applied using the mouse monoclonal antibody 6 isotypes (IgG1, IgG2a, IgG2b, IgG3, IgA, IgM) and monoclonal anti-goat/sheep IgG peroxidase. Protein A column was prepared to test the possibility of separation of the polyclonal antibody to each immunoglobulin class. As a result, the glycosylation between RNase A and B did not have an effect on production pattern of polyclonal antibody from ascites of mice immunized with RNase A or B. Second, in the present study, the polyclonal antibody made from mice immunized with RNase A or RNase B should be isolated to immunoglobulin class or subclass by use of various binding or elution buffer.

사회적 지원이 섬유산업 종사자의 직무스트레스 징후 및 직무성과에 미치는 영향

유화숙,박광희 한국의류산업학회 2005 한국의류산업학회지 Vol.7 No.1

The pm-pose of this study was to investigate social support buffering stress symptoms and the relationship between stress symptoms and job performance. The data were obtained from questionnaires completed by 529 workers employed in textile or clothing companies. The SPSS package was used for data analysis which included t-test, ANOVA, and correlation analysis. The results showed there were significant differences in the perceived levels of social support according to individual differences such as gender, marital status, length of service, title of current position, and division of responsibility. Workers with a high level of supervisor or co-worker social support were found to have the lower levels of stress symptoms. Social support appeared to moderate the relationship between stress symptoms and job performance.

부모권력구조에 대한 아동기 경험이 여대생의 성역할 정체감에 미치는 영향 : The Influence of Childhood Experience of Parental Power Structure

김광응,유미숙,조유진 숙명여자대학교 아시아여성연구소 2004 아시아여성연구 Vol.43 No.1

본 연구는 여대생을 대상으로 부모권력구조에 대한 아동기 경험이 성역할 정체감에 어떤 영향을 미치는지를 알아보고자 하였다. 이를 위해 여대생 425명을 대상으로 부모권력구조에 대한 아동기 경험과 성역할 정체감을 측정한 자료를 분석하였다. 본 연구의 결과를 요약하면 다음과 같다. 첫째, 부모권력구조 각 하위요인(가족행사 · 친족관련 권력,자녀교육 권력, 가정경제 권력)에 대해 부우위형과 모우위형의 부모권력을 경험한 여대생은 성역할 정체감 분포에서도 다른 양상을 보였다. 반면 부모권력구조 척도의 전체점수를 합산하여 부우위형과 모우위형으로 부모권력을 구분했을 때 여대생의 성역할 정체감 분포에는 차이를 보이지 않았다. 이는 기존 부모권력 측정도구의 문제점을 지적한 연구자의 비판에 타당성을 더해주는 결과이다. 둘째, 여대생의 성역할 정체감에 영향을 미치는 변인으로 부모권력구조 하위요인 모두를 동시에 고려한 다반응 로짓분석 결과 부모권력구조 하위요인 중 자녀교육 권력과 가정경제 권력이 유의한 변인으로 나타났다. 즉, 자녀교육 권력에서 아버지가 적극적으로 참여하고 의사결정 정도가 높을 때 여대생들의 양성성과 남성성 성역할 정체감 발달 확률이 높은 반면, 가정경제 권력에서는 어머니의 참여와 의사결정 정도가 높을 때 여대생들이 남성성 성역할 정체감을 발달시킬 확률이 높은 것으로 나타났다. 한편, 가족행사 · 친족 관련 권력은 다른 하위요인의 영향을 제거했을 때 여대생의 성역할 정체감과는 관계가 없는 것으로 나타났다. The purpose of this study was to investigate how sex-role identities of female college students were related to their childhood experiences of parental power structure. Questionnaire survey was conducted with 425 female college students regarding their childhood experiences of parental power structure and their sex-role identities. Parental power structure consisted of three subfactors: parental power on family events/relative-related, child education, and home economics. It was found that the female college students formed different sex-role identity dimensions when their experiences of parental power hierarchy were measured individually in subfactors. However, they did not show differences when their experiences of parental power hierarchy were measured as the sum of subfactor scores. This supports the authors' conviction that previous measures are problematic in identifying relationships of parental power structure and children's sex-role identity. In particular, multi-response logit analysis was used in order to take into account of all the subfactors of parental power structure at the same time. As a result, childhood experiences of parental power hierarchy on child education, and on home economy were found to be closely related to the sex-role identities in the female college students. That is, the female college students, whose fathers had power on their children's education through active participation and decision-making, were likely to develop their androgyny and masculinity. On the other hand, those whose mothers had power in home economy were likely to develop masculinity. Furthermore, family events and relative-related parental power were not found to be related to the sex-role identity in female college students when the impacts of other subfactors were eliminated. It is posited that equal or balanced exertion of parental power is critical in the development of adptive and flexible sex-role identity in their children.

음악영재 판별기준에 대한 탐색 : 영재교육기관의 선발제도 사례를 중심으로

이광숙 순천향대학교 인문과학연구소 2006 순천향 인문과학논총 Vol.18 No.-

당뇨병과 동반된 화농성 간농양의 임상상 및 예후인자

오은숙,강무일,이원영,오기원,임동준,이소영,이정민,고승현,김성래,안유배,손현식,윤건호,차봉연,이광우,손호영,강성구 대한당뇨병학회 2000 임상당뇨병 Vol.1 No.1

연구배경: 감염질환은 혈관질환과 함께 당뇨병의 중요한 사망원인의 하나이며, 혈당조절이 불량할수록 감염 빈도가 증가됨은 잘 알려져 있다. 간농양의 발생이 있어서도 당뇨병은 중요한 기저질환 중의 하나이다. 일반적으로 간농양의 가장 흔한 원인균은 Escherichi coli라고 알려져 있으나, 최근 당뇨병을 가진 간농양 환자에서 Klesiella pneumoniae의 검출이 증가되고 있다. 또 최근 연구에 의하면 K.pneumonie가 간농양의 가장 흔한 원인균이며, K. pneumoniae에 의한 간농양은 K.pneumoniae 이외의 균주에 의한 간농양보다 당뇨병과 밀접히 관련되어 있다고 보고된 바 있다. 이에 저자들은 간농양 환자를 대상으로 당뇨병환자의 빈도를 알아보고, 그 원인균 및 특징을 비당뇨병 환자들의 경우와 비교해 보고자 하였다. 방법: 1992년 1월부터 1999년 6월까지 방사선학적 검사 혹은 수술에 의해 간농양이 확인되거나, 경피적 천자나 수술을 통한 배농액 배양, 혹은 혈액 검사에서 원인균이 증명된 182명의 환자를 대상으로 후향적 임상고찰을 실시하였다. 결과: 화농성 농양을 가진 167명의 환자(남:94명, 여:73명)중 당뇨병을 가진 환자는 54명 (남:30명, 여:24명)으로 32.3%를 차지하였다. 환자의 평균나이는 당뇨군에서 62.1 ± 13.4세, 비당뇨군에서는 55.1 ±15.5세로 당뇨군에서 높았으며(p=0.0021), 남녀간의 평균나이 비교 시 두 군 모두 여성의 나이가 의미있게 높았다. 비당뇨군에 비하여 당뇨군의 경우 저알부민혈증의 빈도와 aspartate transaminase가 증가되어있었다. 원인균주는 K. pneumoniae (당뇨군54%, 비당뇨군 39.1%), E. coli(당뇨군 17.5%, 비당뇨군 18.8%), Pseudomonas aeruginosa, Streptococcus viridans, Enterococcus 순이었으며 두 군간의 의미는 차이는 없었으나 K. pneumoniae의 경우 다른 균종에 비해 단독감염의 빈도가 높았다(82.8%). 사망률은 당뇨군에서 16.7%, 비당뇨군에서 7.1%로 당뇨군에서 의미있게 높았으며(p=0.019), 당뇨군에서의 사망환자는 모두 패혈증이 동반되어 있었다. 당뇨환자의 사망군은 생존군에 비해 당뇨병 이환기간이 길고 혼합감염의 빈도가 높았다(p=0.046). 검사실 소견에서는 총 빌리루빈 및 알카리성 포스파타제의 증가가 관찰되어 있었다. 결론: E. coli가 간농양의 주된 원인균이라는 기존 보고와는 달리 당뇨군과 비당뇨군 모두 K. pneumoniae가 간농양의 주된 원인균이었다. 또한 당뇨 유병기간이 길고 당뇨조절이 불량한 환자에서 혼합감염이며 총 빌리루빈 및 알카리성 포스파타제의 증가등과 같은 위험인자가 존재할 경우 사망률이 증가될 수 있으므로 이들 군에서는 적극적인 치료가 필요할 것으로 사료된다. Background: Liver abscesses are commonly associated with underlying disease, particularly diabete mellitus. The number of the liver abscesses caused by Klebslella pneumoniae in diabetic patient has been increased in Korea nowadays. This study was conducted to clarify the clinical presentation and prognostic factors of pyogenic liver abscesses, especially in diabetic patients and to determine the proportion of K. pneunomiae as a pathogen in liver abscess in Korea. Methods: Medical records of 167 patients treated for pyogenic liver abscess from January, 1992 through June, 1999 were reviewed retrospectively in detail. Major Pathogenic organism, clinical manifestations, prognostic factors, the importance of diabetes mellilus as an underlying disease and its effect on clinical features and prognosis were analyzed. Results: Among 167 cases of pyogenic liver abscess, underlying diabetes mellitus was present in 32.3%. The mean age of patients was 62.7 13.4 years in diabetic liver abscess group and 55.1 15.5 years in non-diabetic liver abscess group. Most liver abscesses were cryptogenic in origin or secondary to the billary tree diseases. The clinical presentations among the two groups were not significantly different. When compared to patients without diabetes, patients with diabetes had significantly higher proportions of hypoalbuminemia and elevated aspartate transaminase level. The most common organism of the pyogenic abscess was K. pneumoniae in both groups. Percutaneous drainage of the abscess with appropriate antibiotics was the most commonly used therapeutic modality in liver abscess. The mortality rate of diabetic liver abscess is 16.1 % and 7.1% in nondiabetic liver abscess. Complications, especially septicemla, were found more frequently in patients with diabetes than in patients without diabetes (64.8% vs 28.3%, septicemia : 31.5% vs 9.7%). The factors influencing mortality in the diabetic liver abscess were disease duration, mixed infection, presence of septicemia, elevated bilirubin and alkaline phosphatase. Conclusions: In contrast to prior report that the E. coli was the most common pathogen in liver abscess, we found that K. pneumoniae was the most common organism cultured in liver abscess. Diabetic patients have more complications and higher mortality than patients without diabetes. Early detection and proper treatment are needed to improve the outcome for diabetic patient with liver abscess.

하악전돌증 환자의 하악운동에 관한 연구

김기숙,김광남 대한두개하악장애학회 1991 대한두개하악장애학회지 Vol.3 No.1

It is important to harmonize the occlusion with TMJ and neuromuscular system. However, clinically, how to harmonize is very difficult. The mandibular movement is the motion in which all component part of stomatognathic system participate. This study was performed to compare mandibular movement of mandibular prognathic patients group with that of normal group, to ascertain which components of mandibular movement have differences between two groups, and to use for occlusal treatment of mandibular movement. Thirteen adult who have physiologically normal occlusion and are free of TMJ dysfunction were selected as a control group(Group l). Eight adult who are mandibular prognathic patient and have more than four anterior teeth crossbite, therefore have not anterior guidance function and have posterior interference at protrusion were selected as a experimental group(Group 2). Electronic pantograph, Denar Pantronic (Denar Corp., U.S.A.), was used to record mandibular movement. Pantronic survey was performed by using an arbitrary hinge axis according to manufacturer's direction. Of the Pantronic recordings, immediate side shift (ISS), progressive side shift (PSS), orbiting condylar path (ORB), protrusive condylar path (PRO) between two groups were compared and analysed. The results were as follows: 1. The average protrusive and orbiting condylar inclination of mandibular prognathic patient(28.44˚, 36.94˚) was significantly lower than those of normal group(40.15˚, 48.00˚) (P<0.01). 2. There was no statistically significant difference between the average immediate and progressive side shift of mandibular prognathic patient (0.37㎜, 6.19˚)and those of normal group(0.52㎜, 5.96˚) (P>0.0l). 3. The significant correlation was found between orbiting condylar inclination and protrusive condylar inclination.

의복행동에 관한 이론적 고찰(Ⅰ) : 의복의 동조성, 유행, 개성, 흥미성, 실용성, 심미성 중심으로 Conformity, Fashionability, Individuality, Interest, Practicality, and Aesthetics

박광희,정인향,최선영,한정숙 啓明大學校 生活科學硏究所 1998 科學論集 Vol.24 No.-

This is the first of two papers in which we review clothing behavior literature found in the field of clothing and textiles. Articles dealt with at least one of six aspects of clothing behavior (conformity, fashionablity, individuality, interest, practicality, and aesthetics) were analyzed 1) to generate each composite definitions of conformity, fashiooablity, individuality, interest, practicality, and aesthetics, 2) to examine scales used from the perspective validity and reliability, and 3) to investigate the results of studies for exploring the possibility of developing new measurements of clothing behavior.

울산시 주민의 건강을 위한 식행동 조사 연구

신애숙,김광기 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.2

This is a descriptive study of consumption patterns of how to take meals in everyday life. The data used was collected from a probability sample by multi-stage cluster sampling, drawn from those who were living in the Ulsan Metropolitan Area. Face-to-face interviews were conducted by trained interviewers with a household member who was between the ages of 15 and 60 in 1989 and had the earliest birthday, resulting in a sample of 1.232 respondents. Univariate and bivariate analyses were made to describe behavioral patterns of taking meals. Meal-taking behaviors under this study include frequency of taking meals per day, regularity of taking meals, which meal to skip, number of frequency of taking a meal by oneself, reasons of skipping and irregular meals, and eating out. Half of the respondents reported to take meals three times a day, while those who skipped at least a meal reported breakfast as one most frequently skipped. Of reasons not to take a meal on a regular basis, "too busy to eat" was most frequently mentioned. Only 32.8% of the respondents reported dining-out once a week.

수종의 암세포주의 저선량 방사선감수성에 관한 실험적 연구

김민숙,고광준 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.2

The purpose of this study was to aid in the radiation therapy of head an neck cancer patients. For this study, radiation survival curves were generated for B16, MG063 and YAC-1 cell lines using semiautomated MTT assay and Dye Exclusion Assay. Irradiation of 2, 4, 6, 8, 10Gy were delivered at room temperature at a dose rate of 210.2cGy/min using ??Co r-ray Irradiator ALDORADO 8. The viable cells were determined for each radiation dose and compared to control values. The obtained results were as follows: 1.There was significantly different absorbance at 10Gy on B16 cell line in MTT assay(P<0.05). 2.There was significantly different absorbance at 4, 6, 8, 10Gy on MG-63 cell line in MTT assay(P<0.05). 3.YAC-1 cell line was more sensitive than B16 or MG-63 cell line to all doses of radiation(P<0.05). 4.There was significantly different absorbance among all tumor cell lines except between B16 and MG-63 cell line at 2Gy in MTT assay(P<0.05). 5.Good correlation was obtained between MTT assay and DEA(P<0.05). The efficient of correlation of B16, MG-63 and YAC-1 cell line was 0.845, 0.824 and 0.906, respectively.