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      • Antifungal activities of zoysiagrass (Zosia japonica Steud.) chitinases against Rhizoctonia solani and analysis of fungus responsive cis-elements in chitinase genes promoter

        Ji-Nam,Kang,So-Mi,Kang,Hong-Gyu,Kang,Hyeon-Jin,Sun,Yong-Ik,Kwon,Suk-Min,Ko,Hyo-Yeon,Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

        Zoysiagrass are damaged by fungi diseases such as large patch, dollar spot, pythium blight and brown patch. Large patch is one of the major diseases caused by Rhizoctonia solani AG2-2 on zoysiagrass fields e.g. golf courses. Plant chitinases have been known PR (Pathogen related)-protein. In this study, we isolated two chitinase genes (Zjchi1 and Zjchi2) from zoysiagrass. Antifungal activity analysis revealed that Zjchi2 protein inhibited mycelium extension of fungi. A further study, we cloned 5` upstream region from two chitinase genes for investigating transcription regulatory mechanism that inducing of two chitinase genes dependent R. solani. -818 bp and -799 of upstream region from Zjchi1 and Zjchi2 successfully isolated using in vitro LA (Long and Accurate) PCR system. And then, we generated promoter-GUS reporter constructs with deletion construct based on W-boxes. Constructs were introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation for stable expression of GUS reporter gene.

      • KCI등재

        Students Perceptions on English Grammar Learning and Instruction in a College

        Kang Hyeon Sook(강현숙) 학습자중심교과교육학회 2016 학습자중심교과교육연구 Vol.16 No.7

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        본 연구는 그 결과를 수업설계와 교과과정에 반영하기 위한 목적으로 대학생들의 영문법 학습과 대학 영문법 강좌에 대한 인식을 살펴 본 것이다. 주관식 응답과 객관식 응답을 구할 수 있는 설문지를 사용하여 조사하였고 토익점수에 따라 상/하반으로 구분하여 인식의 차이가 있는지도 살펴보았다. 학생들은 영어학습에 있어 영문법의 긍정적인 역할에 대해 동의하면서 영문법 학습과 대학에서도 영문법 강의가 필요함을 인정하였다. 문법 학습에 대한 인식에서는 문법용어에 대한 어려움을 호소하였고 특히 수준이 낮은 학생들이 더욱 그러하였으며 문장형식, 어순에 대해 가장 많이 어려움을 토로하였다. 학습방식에서는 규칙을 먼저 학습하는 연역적 학습 방법을 선호하였고 문법 번역식에 대한 의존도가 높았다. 또한 대학에서도 영문법 강좌를 개설해 줄 것을 원하였으나 필수교과목이 아닌 선택교 과목으로서의 개설을 희망하였다. 증가하는 온라인 강좌에 대한 인식도 조사하였는데, 학생들은 오프라인 학습을 선호하였으며, 온라인 강좌에 대한 긍정적, 부정적 이유들도 다양하게 언급되었다. 마지막으로 학생들은 대학에서의 영문법 강좌에 대한 여러 제안을 하였다. 본 연구는 설문항목의 결과만이 아니라 학생들의 다양한 주관식 응답 또한 교수자와 교과과정개발자에게 중요한 시사점을 가진다고 하겠다. The study examined students perceptions on English grammar learning and instruction in a college in order to provide better English learning environment to them. Whether the upper level students have a different idea from the lower level ones are also examined. A questionnaire with open-ended and close-ended questions is used. The participants confirmed the positive role of grammar in improving their language ability, regardless of their proficiency level. They appeal difficulty in understanding the meaning of grammatical terms. Sentence patterns, caused by different word orders between English and Korean, are the most problematic in learning English. Deductive way of learning grammar rules and translation are welcomed in getting grammar instruction. Positive opinion about an opening of a grammar course in a college is confirmed, as an elective one. Their opinion of online and offline learning is also examined. Offline learning is favored, and their reasons for it are given. A few interesting suggestions on a better grammar course are included. Statistical difference between the two groups is not detected in most of the responses, but the upper level group appreciates the role of grammar in improvement of reading and writing more than the lower level group, while lower level group feel understanding grammatical terms more challenging. The results of the study can be all taken into account when practitioners develop English teaching syllabus and curriculum.

      • KCI등재SCOPUS

        The Influence of Gastroesophageal Reflux Disease on Daytime Sleepiness and Depressive Symptom in Patients With Obstructive Sleep Apnea

        ( Hyeon Hui Kang ), ( Chul-hyun Lim ), ( Jung Hwan Oh ), ( Min-jae Cho ), ( Sang Haak Lee ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.2

        Background/Aims As there is insufficient evidence for a relationship between gastroesophageal reflux disease (GERD) and obstructive sleep apnea (OSA), we investigated whether OSA diagnosed by polysomnography (PSG) is related to GERD. Methods A total of 402 subjects was evaluated. Overnight PSG was performed and a few questionnaires on GERD, anxiety, depression, and daytime sleepiness were administered. An apnea-hypopnea index < 5 was the classification criterion for subjects without OSA. Subjects with heartburn or acid regurgitation at least once a week were classified as having GERD. Results Among the 402 subjects, 318 had OSA and 84 did not. The prevalence of GERD was 12.9% among patients with OSA and 10.7% among those without (P = 0.590). The prevalence of GERD did not correlate with OSA severity (P = 0.474). Patients with OSA with GERD had higher Stanford Sleepiness Scale (P = 0.004), Epworth Sleepiness Scale (P = 0.001), and depression (P < 0.001) scores than patients with OSA without GERD. Subjects with nocturnal gastroesophageal reflux symptoms had a higher body mass index, waist-to-height ratio, and waist circumference-to-height index than those without symptoms. Multiple logistic regression showed that higher Epworth Sleepiness Scale and depression scores were independent factors associated with GERD in patients with OSA. Conclusions The prevalence of GERD in patients with OSA was 12.9%. The prevalence of GERD did not correlate with OSA severity. Daytime sleepiness and depression seem to be associated with GERD in patients with OSA, while nocturnal reflux symptoms seem to be related to obesity in OSA. (J Neurogastroenterol Motil 2021;27:215-222)

      • Production and Perception of English /r/ and /l/ by Korean Learners of English : An Experimental Study

        Kang,,Hyeon-Seok 한국음성과학회 1999 음성과학 Vol.6 No.1

        Eleven Korean learners of English took part in a experiment where the production and perception of English /r/ and /1/ in four different word positions was investigated. Overall the subjects made more errors on /1/ in both production and identification test. The frequency of the subjects' errors was also sensitive to word postitions in which the two English liquids occur. Especially the subjects made noticeably fewer errors in intervocalic medial postition. It is suggested that the Korean subjects' acquisitional pattern in this particular case of foreign phone learning can be explained more by language particular 'interference' effects rather than 'universal' acoustic arguments such as those given in Dissosway et al. (1982) and Sheldon and Strange (1982). The results of the experiment also support the minority position among second language educators that in some cases of non-native phone acquistition, learners' production abilities can be developed earlier than their perceptual abilities. Keywords: English liquids, phonological interference, word-positions

      • SCOPUSKCI등재

        In Vivo Neuroprotective Effect of Histidine-Tryptophan-Ketoglutarate Solution in an Ischemia/Reperfusion Spinal Cord Injury Animal Model

        Kang,,Shin,Kwang,Kang,,Min-Woong,Rhee,,Youn,Ju,Kim,,Cuk-Seong,Jeon,,Byeong,Hwa,Han,,Sung,Joon,Cho,,Hyun,Jin,Na,,Myung,Hoon,Yu,,Jae-Hyeon The Korean Society for Thoracic and Cardiovascular 2016 The Korean Journal of Thoracic and Cardiovascular Vol.49 No.4

        Background: Paraplegia is a devastating complication following operations on the thoracoabdominal aorta. We investigated whether histidine-tryptophan-ketoglutarate (HTK) solution could reduce the extent of ischemia/reperfusion (IR) spinal cord injuries in a rat model using a direct delivery method. Methods: Twenty-four Sprague-Dawley male rats were randomly divided into four groups. The sham group (n=6) underwent a sham operation, the IR group (n=6) underwent only an aortic occlusion, the saline infusion group (saline group, n=6) underwent an aortic occlusion and direct infusion of cold saline into the occluded aortic segment, and the HTK infusion group (HTK group, n=6) underwent an aortic occlusion and direct infusion of cold HTK solution into the occluded aortic segment. An IR spinal cord injury was induced by transabdominal clamping of the aorta distally to the left renal artery and proximally to the aortic bifurcation for 60 minutes. A neurological evaluation of locomotor function was performed using the modified Tarlov score after 48 hours of reperfusion. The spinal cord was harvested for histopathological and immunohistochemical examinations. Results: The spinal cord IR model using direct drug delivery in rats was highly reproducible. The Tarlov score was 4.0 in the sham group, $1.17{\pm}0.75$ in the IR group, $1.33{\pm}1.03$ in the saline group, and $2.67{\pm}0.81$ in the HTK group (p=0.04). The histopathological analysis of the HTK group showed reduced neuronal cell death. Conclusion: Direct infusion of cold HTK solution into the occluded aortic segment may reduce the extent of spinal cord injuries in an IR model in rats.

      • KCI등재
      • KCI등재

        Estimating Moisture Content of Cucumber Seedling Using Hyperspectral Imagery

        Kang,,Jeong-Gyun,Ryu,,Chan-Seok,Kim,,Seong-Heon,Kang,,Ye-Seong,Sarkar,,Tapash,Kumar,Kang,,Dong-Hyeon,Kim,,Dong,Eok,Ku,,Yang-Gyu Korean Society for Agricultural Machinery 2016 바이오시스템공학 Vol.41 No.3

        Purpose: This experiment was conducted to detect water stress in terms of the moisture content of cucumber seedlings under water stress condition using a hyperspectral image acquisition system, linear regression analysis, and partial least square regression (PLSR) to achieve a non-destructive measurement procedure. Methods: Changes in the reflectance spectrum of cucumber seedlings under water stress were measured using hyperspectral imaging techniques. A model for estimating moisture content of cucumber seedlings was constructed through a linear regression analysis that used the moisture content of cucumber seedlings and a normalized difference vegetation index (NDVI). A model using PLSR that used the moisture content of cucumber seedlings and reflectance spectrum was also created. Results: In the early stages of water stress, cucumber seedlings recovered completely when sub-irrigation was applied. However, the seedlings suffering from initial wilting did not recover when more than 42 h passed without irrigation. The reflectance spectrum of seedlings under water stress decreased gradually, but increased when irrigation was provided, except for the seedlings that had permanently wilted. From the results of the linear regression analysis using the NDVI, the model excluding wilted seedlings with less than 20% (n=97) moisture content showed a precision ($R^2$ and $R^2_{\alpha}$) of 0.573 and 0.568, respectively, and accuracy (RE) of 4.138% and 4.138%, which was higher than that for models including all seedlings (n=100). For PLS regression analysis using the reflectance spectrum, both models were found to have strong precision ($R^2$) with a rating of 0.822, but accuracy (RMSE and RE) was higher in the model excluding wilted seedlings as 5.544% and 13.65% respectively. Conclusions: The estimation model of the moisture content of cucumber seedlings showed better results in the PLSR analysis using reflectance spectrum than the linear regression analysis using NDVI.

      • KCI등재후보

        Manufacture of Functional Yogurt supplemented with Crude Materials extracted from Kaempferia parviflora

        Kang,,Soo-Hyun,Kim,,Soo-Ki,Kim,,Dong-Hyeon,Kim,,Hong-Seok,Lee,,Soo-Kyung,Song,,Kwang-Young,Yim,,Jin-Hyuk,Kim,,Young-Ji,Kang,,Il-Byung,Jeong,,Dana,Park,,Jin-Hyeong,Jang,,Ho-Seok,Chon,,Jung-Whan,Kim,,Hy Korean Society of Milk Science and Biotechnology 2016 한국유가공기술과학회지 Vol.34 No.1

        Kaempferia parviflora (Krachaidam, Zingiberaceae) is used as a traditional Thai medicine for treating various ailments, including allergy, fatigue, sexual dysfunction, and ulcer. Moreover, it is also used to promote longevity and as a nerve tonic. The aim of this study was to develop functional yoghurt containing Kaempferia parviflora and to examine the physicochemical characteristics of yoghurt supplemented with different concentration of K. parviflora. To this end, we examined the pH and sensory evaluation of yoghurt containing K. parviflora. The pH of this yoghurt decreased whereas the TA increased with incubation time (up to 5 h) without altering the amounts of K. parviflora added. In the sensory evaluation, the taste, flavor, color, and overall acceptability decreased in proportion to the amount of K. parviflora added. Among the experimental group, high scores were achieved by yoghurt containing 1% K. parviflora compared with that by the control group. We conclude there is an urgent need for additional research on the production of this multi-functional yogurt (with properties including anti-obesity and anti-inflammatory), using an optimum concentration of K. Parviflora.

      • KCI등재

        Long Short Term Memory based Political Polarity Analysis in Cyber Public Sphere

        Kang,,Hyeon,Kang,,Dae-Ki The International Promotion Agency of Culture Tech 2017 International Journal of Advanced Culture Technolo Vol.5 No.4

        In this paper, we applied long short term memory(LSTM) for classifying political polarity in cyber public sphere. The data collected from the cyber public sphere is transformed into word corpus data through word embedding. Based on this word corpus data, we train recurrent neural network (RNN) which is connected by LSTM's. Softmax function is applied at the output of the RNN. We conducted our proposed system to obtain experimental results, and we will enhance our proposed system by refining LSTM in our system.

      • Genetic Stability of the Characters Transformed by Microinjection of Exogenous DNA into Crop Plants

        KANG,,NAM-JUN,PARK,,CHAN-HEE,JEONG,,YEON-OK,CHO,,JEOUNG-LAI,KIM,,ZHOO-HYEON,UM,,SUNG-KYUN,PARK,,JUNG-CHOON,KANG,,SEONG-MO 경상대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.7 No.-

        Genetic stability was examined for the second generation(TS-2) progenies of 4 transformants induced by injecting Altari(A) DNA into kungzung(K) radish. All TS-2 plants originated from T3 and T4 had red hypocotyls, suggesting the stable incorporation of the foreign gene and its expression at the second generation. The progenies of these 2 transformants had bigger leaves and heavier roots. However, the segregation of hypocotyl colors of TS-2 plants from T5 and T7 was variable, although the majority still had red hypocotyls. Except the progenises derived from T7, the shapes of cotyledons and the first true leaf, and overall morphology of TS-2 plants as well, were of A types as long as their hypocotyl color was red. For the seedlings to have red hypocotyls, the banding patterns of total suluble proteins and the isozymes of MDH and acid phosphatase should be similar to those of A radish, or at least a mixed type of A and K radish. The later the embryos were removed from the mother plants, and the higher the concn of exogenous DNA up to 10㎍ embryo, the better the differentiation of the explants. The probability to porduce intergeneric hybrids between radish and Chinese cabbage (CC) depended on which was being served as a female. The hybrid plants tended to have mixed banding patterns of total proteins and the isozyme when CC served as a female

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