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Seo, Seung-Bo,Kim, Jae-Ho,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1
내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13 세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13을 2% 가용성 전분, 1% 효모 추출물, 1% Na_(2)CO_(3) 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 50℃에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 65℃, pH 8.5-9.0 이었고 80℃이하와 pH 5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용 시켰을 때 49%의 CD 수율을 보였다. To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isolated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1% Na_(2)CO_(3) (pH 8.5) at 50℃ for 24 h and about 80% of maximal activity was also showed in the culture broth of 60℃ for 18 h . Optimum reaction temperature and pH of the partial purified CGTase for soluble starch were 65℃ and pH 8.5-9.0, respectively. The partial purified CGTase were also stable below 80℃ and pH 5.0-10.0. When 1% soluble starch was digested with the partial purified CGTase, the yield of cyclodextrin was 49%.
동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여
박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1
This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.
박종대,김연보 대구대학교 (한사대학) 산업기술연구소 1994 産業技術硏究 Vol.13 No.-
This paper presents a CMOS intregrated circuit design for measuring simultaneously physiolocial signals from 8 subjects. he implantable system consists of 4 parts; a command receiver, a subject selection receiver, a conditioner and an RF transmitter. Main features of this system are to turn on/of implanted battery-powered system, and to select one among the 8 subjects, and to enable continuous measurement of 8 channel biogical signals from the selected subject and to transmit the biological signals with a double modulation technique. This system, used together with appropriate sensors, is expected to contribute to clinic medicine as pressure, pH, and temperature
스템핑 공정 최적화 시스템의 개발 : 최적블랭크 설계와 최적배치의 일체화 Integration of Optimal Blank Design and Optimal Nesting
박종규,심현보 嶺南大學校 工業技術硏究所 2003 工業技術硏究所論文集 Vol.31 No.1
In recent years, design of an optimal blank shape is very important for sheet metal forming process in the automobile industry because the raw material cost rate is significant part in the automobile industry. With the design of an optimal blank shape, the engineer can protect a blank from an excessive holding force to improve the quality and reduce the ratio of material scrap. Therefore design of an optimal blank shape is inevitable in sheet metal forming process. However, if it causes a complicated shape of blank, it may be difficult to do the blank layout optimally. In this study, we developed software of optimal blank layout connected with the software of optimal blank shape design which was created in the past by the present authors. And by using these softwares, we would like to present the method in order to get optimal utilization ratio easily and precisely within short time for the sequence of works from design to blank layout.
김종보,은종극,박승조 동아대학교 환경문제연구소 1993 硏究報告 Vol.16 No.2
This study has been carried out to confirm the incineration and dewatering ability of the papermaking sludge added pulverized coal. This study purposes was dewatering effeciency of paper-making sludge and autogenous combustion abilities adding heat content analysis as variation coal dosage and particle size. The conclusions from experiment are as follows ; This experiment has been carried out variation of coal dosage. The optimum dewatering efficiency was available in addition 200% pulverized coal as the pulverized coal particle size was from 8 to 10 mesh. The autogenous combustion was possible when pulverized coal more 80% addition to paper-making sludge.