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      • KCI등재

        반월?시화공단 주변 주거지역의 악취현황 및황화합물의 체감도

        김종보,정상진,송일석 한국대기환경학회 2007 한국대기환경학회지 Vol.23 No.2

        This study was carried out to investigate the characteristics of fourteen odor compounds from a total of 10 sam--Sihwa industrial complex. The measurement data are analyzed andcompared with sensation of odor unit. Only a hydrogen sulde out of four sulfur compounds was quantied above the detection limit(0.06ppb) in theresidential area around Banwol industrial complex with leather companies and a sewage treatment plant. The con-centrations of VOCs were higher than those measured from a big city, and styrene showed the relatively high con-~ 37.8ppb). In the case of carbonyl compounds, acetaldehyde was found mostfrequently with the mean of 3.97 ppb, and its concentration difference was not signicant between Banwol andSihwa industrial complex. Of the nitrogen compounds, ammonia was measured at the relatively high concentrationfrom all the sampling sites(12~ 707ppb), and a trimethylamine was found at the odor threshold level(0.1ppb). summer was ve times higher than that in autumn, the concentration of ammonia in autumn was two times higherthan that in summer. However other odorous compounds did not show such strong seasonal variation. Odor-concentration relationship between odor unit and H2S concentrations from industrial sources was examinedand used as odor sensation evaluation, and thus the neighbourhood odor complaints maybe caused during the fourseasons from the results.

      • KCI등재

        整備基盤施設에 대한 有償買入附款의 公定力과 限界 (대상판결: 대법원 2008. 11. 27. 선고 2007두24289 판결)

        김종보 한국행정판례연구회 2009 행정판례연구 Vol.14 No.2

        In urban and living environment improvement act, it is enacted that abolished infrastructure is transferred to developer gratuitously within range of newly-established infrastructure. Especially the range of gratuitous conveyance and the range, cost and time of purchase by compensation are argued. Some suggest that the range of gratuitous conveyance is limited to urban planning facility. But It’s not persuasive opinion because of unfair reduction of the range of gratuitous conveyance. Collateral clause that sets a developer purchase of some abolished infrastructures by compensation is the charge, not the condition. It is disposal that object of litigation for cancellation and it has uncancellable effect and official authority. So civil suit is not allowed, unless litigation for cancellation is filed. Collateral clause on purchase by compensation has official authority, but the nonfulfillment of collateral clause doesn’t cause invalidation of project permission or imposition of rental fee. Therefore, imposition of reparation by reason of the nonfulfillment of collateral clause is illegal. 도시정비법은 정비사업으로 인해 폐지되는 정비기반시설과 설치되는 정비기반시설의 소유권귀속에 관한 규정을 두고 있다. 이에 따르면 새롭게 설치되는 시설의 범위 내에서 폐지되는 시설이 사업시행자에게 무상으로 양도된다. 실무에서는 특히 무상으로 양도되는 시설의 범위, 유상으로 매입해야 하는 시설의 범위와 가액, 매입시기 등이 문제된다. 무상으로 양도되는 정비기반시설의 범위에 관해서는 폐지되는 정비기반시설을 도시계획시설결정이 있는 시설에 한정하는 견해가 있지만 이는 무상양도의 범위를 부당하게 축소하는 것으로 동의하기 어렵다. 폐지되는 정비기반시설의 일부를 사업시행자에게 유상으로 매입하도록 정하고 있는 부관은 조건이 아닌 부담이며, 독립해서 취소소송의 대상이 되는 처분이다. 이 부관에 처분성이 인정되면 그에 따른 불가쟁력과 공정력이 인정되며, 이에 대해 취소소송이 제기되어 취소되지 않는 한 사후에 그 효력을 부인하는 민사상의 청구는 허용되지 않는다. 유상매입부관은 독립한 처분으로 넓은 범위에서 공정력을 갖지만, 그 불이행이 사업시행인가의 효력을 소멸시키거나 사용료의 징수를 정당화하는 데까지 미치는 것은 아니다. 그러므로 유상매입부관의 불이행으로 사업시행자의 점유가 무단점유로 변하는 것은 아니어서 이를 이유로 변상금을 부과하는 것은 위법한 처분이다.

      • 유전자총과 아그로박테리움을 이용한 여러 가지 한국 잔디류의 형질전환체계 확립

        김종보,김경덕,박대섭,Kim Jong-Bo,Kim Kyong-Duck,Park Dae-Sup 한국잔디학회 2004 한국잔디학회지 Vol.18 No.3

        다양한 Zoysiagrass 4가지 품종들을 식물재료로 사용하여 Agrobacterium만 이용한 방법 그리고 particle bombardment로 배발생캘러스에 상처를 낸 후, Agrobacterium으로 공동배양 시키는 2가지 다른 형질전환 방법을 비교하였다. 예비실험에서 일반적으로 형질전환에 널리 사용되는 kanamycin과 PPT(phospinitricin)의 적적선발농도에 대해서 실험하였는데, kanamycin의 경우 300mg/l 그리고 PPT의 경위 50mg/l의 농도에서 가장 효과적인 선발 효율을 나타내었다. Agrobacterium을 이용한 형질전환은 Agrobacterium을 2일간 배양시킨 다음, 박테리아 농도를 O.D 600nm=1.0-1.2로 맞추고, 배발생캘러스를 30분간 간염 시키는 방법이 효과적이었는데, particle bombardment를 이용하여 캘러스에 상처를 유발시킨 후, Agrobacterium으로 감염시키면 3배 이상 높은 형질전환 수율을 획득할 수 있었다. 이상의 결과는 한국잔디 형질전환에 있어서 particle bombardment과 Agrobacterium을 병행하여 실시한 최초의 보고이고, 이러한 시스템을 기반으로 하여 향후 한국잔디를 포함하여 다른 난지형 및 한지형 잔디의 품종개량에 널리 이용되리라 생각된다. In this report, several factors such as infection time and concentration of bacterial suspension, influencing on transient gene expression in Agrobacterium-mediated transformation were evaluated. An appropriate concentration (O.D 600nm = 1.0-1.2) of bateria and 30 min of infection time showed a higher level of GUS expression. To improve transformation efficiency (TE), friable embryogenic calli (FEC) were bombarded by tungsten particles without plasmid DNA, and then co-cultivated with A. tumefaciens LBA4404 which contains pTOK233 super binary vector, carrying neomycin phosphotransferase (NPTII), hygromycin phosphotransferase (hpt) and$\beta-glucuronidase$ (GUS) genes. Three days after co-cultivation with A. tumefaciens and particle bombardment, FEC cultures were transferred to the selection medium (SM: MS medium supplemented with BA 1mg/l, hygromycin 100mg/l, cefotaxime 250 mg/l and vancomycin 200mg/l). They were cultured for 2 weeks and then transferred to the second SM containing hygromycin 50mg/l, cefotaxime 200 mg/l and vancomycin 100mg/l. Later, stable GUS expression was detected 4 to 6 weeks after transfer to the SM. Further, TE from Agrobacterium-mediated transformation after particle bombardment increased to about 3-folds compared with Agrobacterium-mediated transformation without particle bombardment. In the present study, we established an efficient transformation protocol of zoysiagrass by using A. tumefaciens in the combination with particle bombardment for the first time.

      • KCI등재

        Picloram을 이용한 효율적인 백합 캘러스 유도 체계 확립

        김종보,Kim, Jong Bo 국제문화기술진흥원 2021 The Journal of the Convergence on Culture Technolo Vol.7 No.3

        나리는 세계 5대 절화에 속하는 주요 절화 중의 하나이며, 아시아, 유라시아 및 북아메리카 등 다양한 지역에 서식한다. 나리 신품종개발을 위해서는 교배, 돌연변이 및 선발육종 기술 외에 조직배양 기술을 포함하는 생명공학기술 도입을 통한 신품종 육성이 필요한데 조직배양 체계확립은 나리 육종체계에 있어서 필수적인 요소 중 하나이다. 조직배양의 많은 분야 중 배발생캘러스를 이용하여 식물체 재분화 증식 체계확립이 많은 작물에서 연구 중인데 본 연구에서도 배발생캘러스 유도에 사용되는 오옥신 중 picloram을 다양한 농도로 처리하여 최적의 배발생캘러스 유도 농도를 선정하고자 실험을 수행하였다. 실험 결과, 3-4주 후부터 CEC (compact embryogenic callus), FEC (friable embryogenic callus) 및 백색캘러스 이렇게 3가지 형태 캘러스가 발생했는데 1.0 mg/l 처리구에서 CEC와 FEC 모두 높은 효율을 나타내었으나 갈변율이 높은 관계로 0.75 mg/l를 나리 배발생캘러스 유도에 적합한 최적 농도로 선정하였다. 이러한 연구결과는 향후 배발생캘러스를 이용한 나리 식물체 재분화 체계 확립 그리고 우량품종 증식체계 확립에 기여할 것이다. Lily is one of the most important 5 cut flowers in international flower market and lilies are distributed in Asia, Eurasia and North America. To develop a new lily cultivar, in addition to hybridization, mutation and selection methods, biotechnological techniques including tissue culture are also required. Establishment of tissue culture system is one of the requirement for the breeding program in Lily. Among many fields of plant tissue culture, establishment of regeneration system via embryogenic calluses are studied in many crops. In this study, research was carried out to decide the proper concentration of picloram which is used for the induction of embryogenic calluses. As a result, 3 different types of callused were observed after 3-4 weeks. They were CEC (compact embryogenic callus), FEC (friable embryogenic callus) and white callus type. 1.0 mg /l of picloram showed the best result for the production of embryogenic callus, however, due to its higher rate of browning in this concentration, 0.75 mg/l of picloram was selected as a proper concentration of picloram for the induction of CEC and FEC in Lily. These results can be contributed to the establishment of both regeneration system and mass propagation in lily in the future.

      • KCI등재후보
      • KCI우수등재
      • KCI우수등재
      • KCI등재

        행정법학의 개념과 그 외연(外延) ― 제도중심의 공법학방법론을 위한 시론(試論) ―

        김종보 행정법이론실무학회(行政法理論實務學會) 2008 행정법연구 Vol.- No.21

        In the study of administrative law, often the terms administrative law and public law are used interchangeably but there seems to be fundamental differences in the concept and scope between the two. While the concept of the latter derived from the distinction between private and public law, that of the former emerged in the course of the generalization of its study. Such usage of the terms appears to be rooted in the fact that a certain fine-tuning process for the two terms has not yet been properly carried out. Administrative law is a typical branch of public law. It is a field of law whose main purpose is to understand various public law systems of a nation and a community. Therefore, such a purpose needs not be limited to control only the litigable of the systems through administrative litigation and/or judicial review. Rather, the primary task of the study of administrative law should be to understand and to provide bases to design each system, relatively independent from litigability and reviewability. For this reason, the methodology of public law should take, as its subject matters, both administrative process which can be captured by a certain degree of litigation and/or judicial review and objective systems which are hard to be captured in such ways. If this approach is followed, the litigation-oriented legal methodology and the system-oriented legal methodology will mutually organically put together the areas of public law and this will enable a comprehensive understanding of its system. Therefore, the study of administrative law should be a system of legal theory which deals not only with laws relating to 'administration' but also with 'national systems, administrative process and laws relating to public systems.' The national systems which include the legislative and judical systems extend from the legislations relating to the prestige of public law such as national contracts, national property and national liability to various kinds of public law status of local governments. The administrative process, having been the primary subject matter of the general study of administrative law, is the most important subject matter of this field and remains as a logic system focused on administrative dispositions. Lastly, the public systems, despite the limited role of the administrative agencies, do have a very important meaning in public law and include legal personality under public law and the union system. In the study of administrative law, often the terms administrative law and public law are used interchangeably but there seems to be fundamental differences in the concept and scope between the two. While the concept of the latter derived from the distinction between private and public law, that of the former emerged in the course of the generalization of its study. Such usage of the terms appears to be rooted in the fact that a certain fine-tuning process for the two terms has not yet been properly carried out. Administrative law is a typical branch of public law. It is a field of law whose main purpose is to understand various public law systems of a nation and a community. Therefore, such a purpose needs not be limited to control only the litigable of the systems through administrative litigation and/or judicial review. Rather, the primary task of the study of administrative law should be to understand and to provide bases to design each system, relatively independent from litigability and reviewability. For this reason, the methodology of public law should take, as its subject matters, both administrative process which can be captured by a certain degree of litigation and/or judicial review and objective systems which are hard to be captured in such ways. If this approach is followed, the litigation-oriented legal methodology and the system-oriented legal methodology will mutually organically put together the areas of public law and this will enable a comprehensive understanding of its system. Therefore, the study of administrative law should be a system of legal theory which deals not only with laws relating to 'administration' but also with 'national systems, administrative process and laws relating to public systems.' The national systems which include the legislative and judical systems extend from the legislations relating to the prestige of public law such as national contracts, national property and national liability to various kinds of public law status of local governments. The administrative process, having been the primary subject matter of the general study of administrative law, is the most important subject matter of this field and remains as a logic system focused on administrative dispositions. Lastly, the public systems, despite the limited role of the administrative agencies, do have a very important meaning in public law and include legal personality under public law and the union system.

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