Four new compounds from the bulbs of Lycoris aurea with neuroprotective effects against CoCl2 and H2O2-induced SH-SY5Y cell injuries

An Jin,Xuelian Xiang,Yun-Yun Zhu,Heng-Yi Yu,Hui-Fang Pi,Peng Zhang,Han-Li Ruan 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

Three new alkaloids, 2a-hydroxy-6-O-n-butyloduline,O-n-butyllycorenine, (-)-N-(chloromethyl)lycoramine(1–3), and a new phenolic compound, ((7S)-7-(4-hydroxyphenyl)-7-hydroxypropyl)-20-methylbenzene-30,60-diol (14), along with ten known alkaloids (4–13), wereisolated from the bulbs of Lycoris aurea collected fromHuaihua County of Hunan Province, China. Their structureswere elucidated by spectroscopic methods includingHRESIMS, UV, IR, and NMR. All the isolated compoundswere tested for their neuroprotective effects against CoCl2and H2O2-induced SH-SY5Y cell death. Compounds 1–7and 10 exhibited significant neuroprotective effects againstCoCl2-induced SH-SY5Y cell injury, while compounds1–5, 7, 10 and 12 showed obvious neuroprotective effectsagainst H2O2-induced SH-SY5Y cell death.

BCG 접종후의 결핵반응검사의 변화

김진경,김기홍,김행미,안두홍 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

신생아 시기에 BCG를 접종받은 영아를 대상으로 3±개월, 7-8개월 및 15-16개월에 Mantoux 검사를 시행하여 다음과 같은 결과를 얻었다. 3-4개월째 측정한 결절의 크기는 5.8±4.5㎜, 7-8개월째 7.9±4.9㎜,15-16개월째 6.7±4.9㎜로서 7-8개월째 의의있게 (p<0.55)증가되어 있었다. Mantoux 검사상 결절의 크기가 5㎜미만인 경우를 음성으로 간주하였을대 3-4개월째 38.5%, 7-8개월째 26.0% 및 15-16개월째 35.2%가 음성이었으며 15㎜이상의 강양성은 3-4개월째 1.9%, 7-8개월째 12%, 15-16개월째 7.5%를 차지하였다. 33례의 미숙아에 있어서 BCG 접종후 18개월 동안 5㎜ 이상의 반응을 보인 양전례는 63.6%인 21례로 이들 양전군과 비양전군 사이에는 출생시 제태연령, 체중 및 BCG 접종시기에 유의한 차이를 보이지 않았으며 만삭아의 양전율과 비교 하였을 때 의의있는 차이를 보이지 않았다. We conducted this study to evaluate the change of tuberculin reaction after neonatal BCG vaccination in Korea. Seventy-four infants were followed for 15-16 month after birth. BCG vaccination were given within 4 weeks after birht and 3 times of Mantoux test were performed at 3-4 months, 7-8 months and 15-16 month after birth. During the same period, Mantoux test were done on 238 cases of 3-4 months-old infants, 107 cases of 7-8 months-old infants and 85 cases of 15-16 months-old infants who were given BCG vaccination within 4 week after birth. We evaluated the results without distinction of these infants because there was no statistically significant difference between two groups. The size of induration was significantly increased at 7-8 months(7.9±4.9㎜) compare to the size at 3-4 months (5.8±4.5㎜) and 15-16months(6.7±4.9㎜) (P<0.05). But 38.5%, 26.8% andn 32.5% of infants were not converted ( < 5㎜) at 3-4months, 7-8 months and 15-16 months after birth respectively. Thirty-three preterm infants were followed for 18 months after birth. Twenty-one out of 33 (63.6%) were converted during this period. There was no difference of gestational age, birth weight, age on BCG vaccination between converters and nonconverters. There was no difference of conversion rate compatre to full term infants.

The roles of interleukin-17A in risk stratification and prognosis of patients with sepsis-associated acute kidney injury

Heng Jin,Wei Wei,Yibo Zhao,Ai Ma,Keke Sun,Xiaoxi Lin,Qihui Liu,Songtao Shou,Yan Zhang 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.6

Background: The aim of this study was to evaluate the roles of interleukin (IL)-17A in risk stratification and prognosis of patients with sepsis-associated acute kidney injury (SAKI). Methods: We enrolled 146 sepsis patients (84 non-SAKI and 62 SAKI patients) admitted to the emergency department from November 2020 to November 2021. Patients with SAKI were differentiated based on the severity of acute kidney injury. All clinical parameters were evaluated upon admission before administering antibiotic treatment. Inflammatory cytokines were assessed using flow cytometry and the Pylon 3D automated immunoassay system (ET Healthcare). In addition, a receiver operating characteristic (ROC) curve was utilized to determine the prognostic values of IL-17A in SAKI. Results: The levels of creatinine, IL-2, IL-4, IL-6, IL-17A, tumor necrosis factor alpha, C-reactive protein, and procalcitonin (PCT) were significantly higher in the SAKI group than in the non-SAKI group (p < 0.05). The level of IL-17A revealed significant differences among stages 1, 2, and 3 in SAKI patients (p < 0.05). The mean levels of PCT, IL-4, and IL-17A were significantly higher in the non-survival group than in the survival group in SAKI patients (p < 0.05). In addition, the area under the ROC curve of IL-17A was 0.811. Moreover, the IL-17A cutoff for differentiating survivors from non-survivors was 4.7 pg/mL, of which the sensitivity and specificity were 77.4% and 71.0%, respectively. Conclusion: Elevated levels of IL-17A could predict that SAKI patients are significantly prone to worsening kidney injury with higher mortality. The usefulness of IL-17A in treating SAKI requires further research.

A Novel Monoclonal Antibody Induces Cancer Cell Apoptosis and Enhances the Activity of Chemotherapeutic Drugs

Xu, Heng,Tian, Yan-Na,Dun, Bo-Ying,Liu, Hai-Tao,Dong, Guang-Kuo,Wang, Jin-Hua,Lu, Shang-Su,Chen, Bo,She, Jin-Xiong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

A novel monoclonal antibody (mAb), known as AC10364, was identified from an antibody library generated by immunization of mice with human carcinoma cells. The mAb recognized proteins in lysates from multiple carcinoma cell lines. Cell cytotoxicity assays showed that AC10364 significantly inhibited cell growth and induced apoptosis in multiple carcinoma cell lines, including Bel/fu, KATO-III and A2780. Compared with mAb AC10364 or chemotherapeutic drugs alone, the combination of mAb AC10364 with chemotherapeutic drugs demonstrated enhanced growth inhibitory effects on carcinoma cells. These results suggest that mAb AC10364 is a promising candidate for cancer therapy.

한-중 과학영재학교 교육과정 비교고찰

김금희 ( Jin Ji Jin ),심재영 ( Jae Young Shim ),김언주 ( Ohn Joh Kim ),조대항 ( Da Heng Zhao ) 충남대학교 교육연구소 2008 교육연구논총 Vol.29 No.1

영재는 어느 한 분야에서 일반학생과 다른 능력을 가진 아동 이므로 영재들에게 적합한 차별화된 교육과정이 필요하다. 현재 세계 여러 나라들의 영재교육과정을 종합해보면 크게 두 형태로 나뉜다. 하나는 심화를 중심으로 하는 교육과정이고, 다른 하나는 속진을 위한 교육과정 설계이다. 이 두 형태는 완전히 다른 교육과정으로서, 각각의 장점과 단점을 갖고 있다. 현재, 한국은 주로 영재에 대한 심화교육과정을 실시하고 있고, 중국은 속진적인 교육과정을 실시하고 있다. 교육현장의 실천을 통하여 영재에 대한 심화 혹은 속진 등 한 가지의 단일형태의 교육과정으로는 영재들의 빠른 학습능력과 습득 지식의 수요량 등 면에서의 요구를 만족시킬 수 없다. 본 연구는 우선 두 나라의 심화와 속진의 영재교육과정의 특성을 분석하고, 다음은 두 나라의 영재들에게 더욱 적합한 영재교육과정을 제공하기 위해 개선해야 하는 점과, 보완해야 하는 점을 모색해봤다. 한국과 중국은 현재의 심화 혹은 속진 영재교육과정의 단일한 구조를 벗어나 이 두 가지를 모두 고려하는 영재교육과정을 설계, 실시해야 할 것이며, 동시에 영재들의 조화로운 사회성 및 인간적 육성을 위한 노력도 해야 할 것이다. The gifted has different characteristics compare to general students. It is very important how and what we offer appropriate curriculum for the gifted. There are two types of curriculum for the gifted. One is enrichment and the other is acceleration. They are much different and have merits and faults each. Particularly in Korea, they usually put enrichment curriculum for the gifted but in China, the acceleration type is more popular than the others. but both of two county just put the one type. It`s not easy to satisfy needs for the gifted who has excellent learning ability. We studied the two types of curriculum in both country. And we tried to figure out ideas to solve recent problems, to improve curriculum, and to develop sociality and personality for the gifted.

Biruloquinone, an Acetylcholinesterase Inhibitor Produced by Lichen-Forming Fungus Cladonia macilenta

( Luo Heng ),( Chang Tian Li ),( Jin Cheol Kim ),( Yan Peng Liu ),( Jae Sung Jung ),( Young Jin Koh ),( Jae Seoun Hur ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2

At present, acetylcholinesterase (AChE) inhibitors are the first group of drugs to treat mild to moderate Alzheimer`s disease (AD). Although beneficial in improving cognitive and behavioral symptoms, the effectiveness of AChE inhibitors has been questioned since they do not delay or prevent neurodegeneration in AD patients. Therefore, in the present study, in order to develop new and effective anti-AD agents from lichen products, both the AChE inhibitory and the neuroprotective effects were evaluated. The AChE inhibitory assay was performed based on Ellman`s reaction, and the neuroprotective effect was evaluated by using the MTT method on injured PC12 cells. One AChE inhibitor (IC50 = 27.1 μg/ml) was isolated by means of bioactivity-guided isolation from the extract of lichen-forming fungus Cladonia macilenta, which showed the most potent AChE inhibitory activity in previous screening experiment. It was then identified as biruloquinone by MS, and 1H- and 13C-NMR analyses. The inhibitory kinetic assay suggested that biruloquinone is a mixed-II inhibitor on AChE. Meanwhile, biruloquinone improved the viability of the H2O2- and β-amyloid-injured PC12 cells at 1 to 25 μg/ml. The protective effects are proposed to be related to the potent antioxidant activities of biruloquinone. These results imply that biruloquinone has the potential to be developed as a multifunctional anti- AD agent.

Mechanical and electrical properties of epoxy resin/epoxidized castor oil/carbon fiber cloth composites

Fan-Long Jin,Heng Zhang,Shan-Shan Yao,Soo-Jin Park 한국탄소학회 2017 Carbon Letters Vol.22 No.-

Synthesis and Properties of Cellulose-based Superabsorbent Hydrogel by a New Crosslinker

Heng-Xiang Li,Xin Tian,Luming Zhang,Leili Wang,Li’e Jin,Qing Cao 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

Cellulose-based superabsorbent hydrogel (SAH) has been successfully prepared by the grafting method withcellulose and sodium acrylate as well as aluminum hydroxide as a novel crosslinker. The factors influencing water absorptionsuch as the neutralization degree of acrylic acid (AA), the amount of initiator ammonium persulfate (APS) and crosslinker,and the proportion of cellulose to AA were investigated. The chemical structure and thermal behavior of the SAH wereanalyzed by fourier-transform infrared spectrometry, field-emission scanning electron microscopy, and thermal gravimetry,respectively. Results showed that the water absorbency of the SAH in distilled water and 0.9 wt% NaCl solution reached 922and 118 g/g when the mass ratio of cellulose to AA, APS to AA, Al(OH)3 to AA, and the neutralization degree of AA was39 wt%, 0.67 wt%, 0.89 %, and 76 %, respectively. Under the same condition, the hydrogel synthesized by traditionalcrosslinker N,N-methylenebisacrylamide was only 530 and 84 g/g in distilled water and 0.9 wt% NaCl solution, respectively. The mechanism of polymerization reaction was analyzed. And the cost and feasibility for the mass-producing the hydrogelwere analyzed.

Genome editing in potato plants by agrobacterium-mediated transient expression of transcription activator-like effector nucleases

Jin Ma,Heng Xiang,Danielle J. Donnelly,Fan-Rui Meng,Huimin Xu,Dion Durnford,Xiu-Qing Li 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.5

Genome editing (also known as targeted mutation) has promise for molecular breeding. Compared with the CRISPR/Cas9 system, the transcription activator-like effector nucleases (TALENs) have likely a lesser off-target rate in genome editing. Both a rapid test system for the functionality of designed TALENs and an effective delivery system for introducing the TALENs to plants are critical for successful target mutation. TALENs have usually been tested in protoplasts or introduced to plants with viral vectors. However, plant regeneration from protoplast culture can generate extensive somatic variation. Viral vectors are not always available, and plants edited by these vectors usually require virus elimination. Here, we used a nonviral, Agrobacterium-mediated transient expression approach, to serve both rapid test and effective delivery of TALENs into two vegetatively propagated potato cultivars, Solanum tuberosum Russet Burbank and Shepody. Two TALENs with different molecular weights (22 and 27 aarepeat modules) were expressed to target two endogenous genes (starch branching enzyme and an acid invertase) by Agrobacterium-mediated infiltration (agroinfiltration) into leaves of potato plants. The infiltrated leaf DNA was analyzed using restriction site loss assay and subsequent DNA sequencing. Deep sequencing of these tetraploid cultivars was also conducted to determine the zygosity at the targeted chromosomal loci. TALENs, with different molecular weights, successfully agroinfiltrated and induced mutations at both targeted loci.

Optimization of a Fluid Distribution Manifold

Heng Wang,Samuel D. Marshall,Rerngchai Arayanarakool,Lakshmi Balasubramaniam,Xin Jin,Poh Seng Lee,Peter C. Y. Chen 한국유체기계학회 2018 International journal of fluid machinery and syste Vol.11 No.3

This paper presents two optimized designs of a commonly-used fluid distribution manifold having one entrance and six exits. Numerical simulations were carried out to optimize the dimensions and mechanisms of these proposed designs for the sake of enhancing the uniformity of fluid distribution amongst the exits and reducing the formation of dead zones inside the manifold cavities. Particularly, to make the fluid distribution amongst exits more uniform, this study explored the relationship between entrance diameter and exit diameter. Furthermore, in order to reduce dead zone formations inside the manifold whilst still maintaining uniform fluid distribution, a conical cavity was designed. After that, blockers were designed to replace some exits, permitting a variable number of fluid distribution manifold exits, depending on the specific application. Both designs were found to be able to improve flow uniformity and dead zone reduction compared to the original commonly-used fluid distribution manifold, with the central-feeding distributor performing slightly better than the lateral-feeding distributor overall. From the perspective of manufacturing, each of these two fluid manifolds was made of two pieces with glue and rubber O ring used respectively as the bond between separate pieces. Preliminary experiments with these devices suggest similar results to those from the numerical studies. Based on real application requirements and limitations, the different fluid manifold designs with tunable dimensions can be utilized in various mechanical or biochemical devices to distribute fluid equally amongst several parallel components.