The novel herbal cocktail MA128 suppresses tumor growth and the metastatic potential of highly malignant tumor cells

KIM, AEYUNG,IM, MINJU,YIM, NAM-HIU,HWANG, YOUN-HWAN,YANG, HYE JIN,MA, JIN YEUL Spandidos Publications 2015 ONCOLOGY REPORTS Vol.34 No.2

<P>MA128, a novel herbal medicine, was previously identified and its effectiveness in the treatment of asthma and atopic dermatitis (AD) was demonstrated. In particular, post-inflammatory hyperpigmentation (PIH) in AD mice was improved by treatment with MA128. In addition, MA128 exhibited anti-melanogenic activity by inhibiting tyrosinase activity via the p38 MAPK and protein kinase A signaling pathways in B16F10 cells. In the present study, we examined whether oral administration of MA128 suppressed the in vivo tumor growth of HT1080 cells in athymic nude mice. The results showed that the daily oral administration of 75 and 150 mg/kg MA128 suppressed the tumorigenic growth of HT1080 cells efficiently. Since metastasis is a major cause of cancer-associated mortality and the greatest challenge during cancer treatment, we investigated the effect of non-toxic concentrations of MA128 on the metastatic potential of HT1080 cells. MA128 inhibited anchorage-independent colony formation, migration and invasion. Matrix metalloproteinase-9 (MMP-9) activity under resting and PMA-stimulated conditions was decreased in a dose-dependent manner by MA128 in HT1080 cells. In addition, the daily oral administration of MA128 at doses of 75 and 150 mg/kg efficiently blocked the lung metastasis of B16F10 cells that had been injected into the tail veins of C57BL/6 mice. In particular, none of the mice treated with MA128 exhibited systemic toxicity, such as body weight loss or liver and kidney dysfunction. MA128 also inhibited tumor-induced angiogenesis. Taken together, the results suggest that MA128 is a potential therapeutic agent and a safe herbal medicine for controlling malignant and metastatic cancer.</P>

Anti-melanogenic activity of the novel herbal medicine, MA128, through inhibition of tyrosinase activity mediated by the p38 mitogen-activated protein kinases and protein kinase signaling pathway in B16F10 cells

Kim, Aeyung,Ma, Jin Yeul Medknow PublicationsMedia Pvt Ltd 2014 Pharmacognosy magazine Vol.10 No.39

<P><B>Background:</B></P><P>Recently, our research group developed MA128, a novel herbal medicine, and demonstrated that MA128 is effective for the treatment of asthma and atopic dermatitis (AD). In particular, postinflammatory hyper-pigmentation in AD mice was improved with MA128 treatment. Thus, in this study, we determined the effect of MA128 on melanogenesis and its underlying mechanism in murine B16F10 melanoma cells.</P><P><B>Materials and Methods:</B></P><P>After treatment with MA128 at 100 and 250 μg/mL and/or alpha-melanocyte stimulating hormone (α-MSH) (1 μM), cellular melanin content and tyrosinase activity in B16F10 cells were measured. Using western blotting, expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF), and activation of c-AMP-dependent protein kinase (PKA), c-AMP-related element binding protein (CREB) and mitogen-activated protein kinases (MAPKs) were examined.</P><P><B>Results:</B></P><P>MA128 significantly inhibited melanin synthesis and tyrosinase activity in a resting state as well as α-MSH-stimulating condition, and significantly decreased the expression of tyrosinase, TRP-1, TRP-2 and MITF. In addition, phosphorylation of PKA and CREB by α-MSH stimulation was efficiently blocked by MA128 pretreatment. Moreover, MA128 as an herbal mixture showed synergistic anti-melanogenic effects compared with each single constituent herb.</P><P><B>Conclusion:</B></P><P>MA128 showed anti-melanogenic activity through inhibition of tyrosinase activity mediated by p38 MAPK and PKA signaling pathways in B16F10 cells. These results suggest that MA128 may be useful as an herbal medicine for controlling hyper-pigmentation and as a skin-whitening agent.</P>

Oral Administration of Novel Oriental Medicine, KIOM-C, Protect against Influenza Virus

Jin Yeul Ma, Eun Ha Kim, Jun Han Lee, Min-Suk Song, Yun Hee Baek, Young Ki Choi 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

The influenza virus is an important respiratory risk affecting humans, and effective treatments are needed. Some oriental medicines are currently applied for treatment of common colds as well as influenza infection. Previous studies have reported that the therapeutic properties of MA-128 are effective for treatment of psoriasis antiasthmatic and atopic dermatitis. In this study, we investigated the therapeutic properties of the novel herbal medicine, MA-128, for treatment of influenza virus infection by oral administration. MA-128 is an active natural biological compound from herbal-marine origin. The results showed that oral administration of MA-128 in mice could confer a survival benefit against Type A influenza virus infection. Daily oral administration of MA- 128 resulted in delayed death in infected mice for three days against mouse adapted H3N2 (A/Philippines/2/82). However, it protected more than 60% of mice from lethal infection of 2009 pandemic H1N1 (A/Korea/CJ01/2009) influenza virus. In addition, lung viral titers were significantly reduced at seven days post infection (~100 times) compared with mock-treated mice and viruses were cleared at 9 dpi only in the MA-128 treated groups. This study demonstrated the potential of the novel herbal medicine, MA-128, as an herbal remedy against influenza A viruses.

농업인 교육 프로그램 분석

김진모,김진군,마상진,전영욱,이진화,주현미 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구는 농업인을 대상으로 한 교육 프로그램의 내용 및 방법을 살펴봄으로써 향후 농업인 교육을 위한 기초자료를 제공하는 데 목적이 있었다. 농업인 교육프로그램 분석을 위한 개념적 틀은 관련문헌에 대한 고찰과 수집 자료에 대한 귀납적 분석을 통해 개발되었다. 그 결과, 8가지의 기관 유형, 17가지의 프로그램 내용 영역, 5가지의 교육방법, 3가지의 교육인원, 3가지의 교육기간이 도출되었다. 분석대상은 총 173개 기관의 2,483개 프로그램이었다. 이 연구의 주요 결과는 다음과 같다. 첫째, 우리나라의 농업인 교육은 국가기관 및 기술센터 등의 정부기관 주도로 이루어지고 있었다. 둘째, 재배/사육기술, 생활개선 및 농촌건강관리, 정보획득 등의 내용이 주요 교육내용으로 다루어지고 있었다. 셋째, 각 기관 유형별로 비중 있게 다루는 주요 교육내용에 있어서 다소 차이가 있었다. 넷째, 농업인 교육프로그램에서 활용되는 교육방법은 대체적으로 강의에 편중되어 있었다. 다섯째, 교육인원에 있어 중규모와 대규모 인원의 비중이 상대적으로 높았으며, 1 대 1 개별 교육은 전혀 없는 것으로 나타났다. 여섯째, 1일 혹은 3일 이상 이루어지는 교육의 비중이 상대적으로 높았으며, 2일 동안 이루어지는 교육의 비중은 비교적 낮은 편이었다. The purposes of this study were to analyze the farmer education programs in Korea, and to get implications for further program development. The framework for the analysis was developed through extensive literature review and an inductive method. As a result, eight types of organizations for farmer education, seventeen content domains of programs, five instructional methods, three categories of program participant size, and three categories of program period were derived. The subjects of analysis were 2,483 programs of 173 farmer education organizations. The major findings were as follows: 1) Farmer education programs of Korea were led by government agencies such as national organizations and extension agencies. 2) The content domains of cultivating/raising techniques, improvement of life quality and farmer health care, and information acquisition were relatively highly dealt with. 3) Main contents which were dealt with by each type of farmer education organization were differentiated. 4) In terms of instructional method, a lecture had the highest portion in most contents domains. 5) The portion of the middle size and large size of participants was relatively higher than others. And there are no programs which used one by one teaching method. 6) The portion of programs for 1 day or 3days was relatively higher, and the portion of programs for 2 days was lower.

龜尾地域의 工業化에 따른 諸問題點과 그 對策에 關한 硏究 : 經濟的 側面 an economic perspective

馬鎭浩 慶北大學校 東洋文化硏究所 1977 東洋文化硏究 Vol.4 No.-

This study explores and analyze some problems in the process of industrialization of Gumi area and then recommends some preparatory measures to cope with these circumstances. Chapter One present an introductory role to understanding the Gumi industrial complex by tracing its history, location and present conditions. Chapter Two explores the socio-economic effects brought about by industrialization of Gumi Area in three parts: (1) the effects on employment; (2) the effects on income growth; (3) the effects on export increase. The effects on employment enlargement goes to the extent that the industrialization of the Gumi area has greatly reduced the disguised unemplaymenis which had been existed in this once rural area; especially the reduction is remarkable for the unmarried female labor force who are the main man-power of the industrial complex. Not only those unmarried female laborers in this community but also those in the neighboring communities are diffused to be employed. At present it is estimated that this portion of labor force are almost fully employed. In the second place, the industrialization of the Gumi area has made a great contribution to the income growth of the people of this area. Despite the existing factors that might cause to decrease the income level such as the erosion of arable land, the increase of tax burden, and the rise of prices of commodity, it is estimated that the industrialization of the Gumi area has brought increase in income of the people of the area due to the increase of the employment, increase in property income owing to the rise of property prices and prosperity in various kinds of service industry. Therefore, factors leading to the increase of income overwhelm those leading to the opposite. Thus, the net increase of income per year is estimated to reach about five billions won in total which means 200,000 won of yearly income per household of this area. Thirdly, goal achievement has not been satisfactory in the area of export increment effect. Despite its purpose as an export industrial complex, only one third-of its total products has been exported. Out of 100 operating companies, twentyfour companies have never exported their production to foreign countries. Chapter Three analytically discusses various problems caused by industrilaizing Gumi area in terms of three perspectives: (1) efficiency aspect of the Gumi industrial complex management; (2) the problems of supporting projects (3) supply and demand of labor forces. The chapter also presents some measures to cope with these problems. First, in the discussion of the efficiency of the industrial complex management, several points including inefficiency of land use, unsatisfactory condition of export, lack of cooperation and production line arrangement between firms have been discussed with the proposal of some recommendations to remedy these conditions and problems. Secondly, problems pointed toward the supporting projects include the shortage of transportation instruments, housing and others. These problems are seriously discussed with the proposal of recommendations to be implemented both in short term and long term. Thirdly, the discussion of the problems of supply and demand in labor force analyzes and explores the prospective demand of labor force and the possibility of supply of labor force. It is expected that the near future will see shortage of labor force. Some measures to meet these problems have been proposed both on a short term and long term basis. Lastly in the concluding chapter, we have discussed some difficulties found in the process of industrialization of the Gumi area and summarizes our recommendations, We have conclusively made it clear that achieving a smooth progress in the industrialization of this area, most of all, requires formation of background cities surrounding the Gumi area.

轉換期에 있어서의 韓國 重化學工業의 輸出戰略

馬鎭浩 慶北大學校 經商大學 經濟學科 1978 慶商大學論集 Vol.6 No.-

平價切下와 貿易收支

馬鎭浩 慶北大學校 經商大學 經濟學科 1975 慶商大學論集 Vol.3 No.-

海外直接投資가 貿易에 미치는 效果

馬鎭浩 慶北大學校 經商大學 經濟學科 1983 慶商大學論集 Vol.11 No.-

南北問題 解決에 關한 一視角

馬鎭浩 慶北大學校 經商大學 經濟學科 1981 慶商大學論集 Vol.9 No.-

EC地域에 대한 投資家能性과 展望

馬鎭浩,崔炳龍,孫炳海,李東萬 慶北大學校 經商大學 經濟學科 1979 慶商大學論集 Vol.7 No.-