Disparate apoptotic activities of the adult-type granulosa cell tumor-associated FOXL2 mutant (C134W) and its wild-type FOXL2

Jae-Hong Kim,Seongmin Yoon,Mira Park,Ho-Oak Park,Jeong-Jae Ko,Sunyoung Lee,Kangseok Lee,Jeehyeon Bae 한국발생생물학회 2010 한국발생생물학회 학술발표대회 Vol.29 No.-

Some mutations in FOXL2 are responsible for premature ovarian failure accompanied with blepharophimosis-ptosis-epicanthusinversus syndrome (BPES) typeI disease, and FOXL2-null mice exhibit developmental defects of granulosa cells. Recently, a new somatic mutation in FOXL2,c.402C>G, leading top. C134W change, has been identified in a vast majority of adult-type ovarian garnulosa cell tumors (GCTs). In the current study, we investigated possible mechanisms by which the C134W mutation could contribute to GCT development. The wild-type (WT) FOXL2 and its mutant form displayed differential apoptotic activities, in which WT induced a significant granulosa cell death while the mutant exhibited a minimal cell death effect. The FOXL2-induced apoptotic response was greatly dependent on caspase8, BID, or BAK since the depletion of either of them prevented FOXL2 to elicitits full apoptotic responses. Stimulated activation of caspase8, consequently resulting increased production of truncated BID (tBID), up-regulation and oligomerization of BAK, and release of cytochromec were all associated with the apoptosis followed by WT FOXL2 expression. In contrast, the mutant FOXL2 was deficient to elicit the full apoptotic signaling responses. In addition, we found the differential up-regulations of expression of death receptors including Fas and TNF-R1 between the WT and the mutant. Moreover, granulosa cells expressing either the WT FOXL2 or its variant form (C134W) exhibited distinct cell death sensitivities by the activation of death receptors. Thus, these differential activities of FOXL2 and it mutant may partly account for the pathophysiology of GCT development occurred by the somatic mutation (C134W) of FOXL2.

폐경 전, 폐경 여성에 있어서 체 성분과 골밀도와의 연관성

정재은 ( Jae Eun Chung ),황성진 ( Sung Jin Hwang ),김민정 ( Min Jung Kim ),송재연 ( Jae Yen Song ),조현희 ( Hyun Hee Cho ),권동진 ( Dong Jin Kwon ),유영옥 ( Young Oak Lew ),임용택 ( Young Taik Lim ),김은중 ( Eun Jung Kim ),김장흡 대한폐경학회 2010 대한폐경학회지 Vol.16 No.1

연구목적: 본 연구는 폐경 전과 폐경 여성에서의 신체구성 (body composition) 중 체지방, 제지방 및 다른 인자와 요추골밀도 및 대퇴골 경부 (femoral neck), ward 삼각주, 대퇴골 전자부위 (trochanter), 대퇴골 전체 (total) 골밀도 (bone mineral density, BMD)의 상관관계를 알아보았다. 연구재료 및 방법: 가톨릭대학교 의과대학 서울 성모병원 건진 의학과 건진 센터에서 건강검진을 시행한 여성 394명을 대상으로 체 성분 및 지질, 공복혈당, 혈압, 골밀도 등을 측정하였다. 골밀도는 양 에너지 방사선 흡수계측 기 (dual energy X-ray absorptiometry, DXA)를 이용하여 요추 골밀도와 대퇴골 경부, ward 삼각주, 대퇴골 전자부위, 대퇴골 전체의 골밀도를 측정하고, bioimpedence 기계를 사용하여 체 성분을 분석하였다. 골밀도와 각 변수들과의 연관성은 통계적 분석 SPSS (for windows 13.0, SPSS Inc. Chicago, IL, U.S.A.)을 이용하여, bivariable correlation analysis로 분석하였다. 교란변수 보정을 위해서는 부분상관분석 (partial correlation) 분석을 이용하였다. P값이 0.05 미만인 경우를 통계학적 의미가 있는 것으로 정의하였다. 결과: 대상 환자의 평균나이는 49.5±9.8세였고, 이중 51.5%인 203명이 폐경 전 여성, 48.5%인 191명이 폐경 여성이었다. 폐경전 여성에서 나이와 체중을 보정하고도 요추 골밀도 및 대퇴골 경부, ward 삼각주, 대퇴골 전자부위(trochanter), 대퇴골 전체 (total) 골밀도는 근육량, 제지방량, 부위별 근육량, 기초대사량과 양의 상관관계를 보였으며, 복부지방률, 체지방량, 허리둘레, 체지방률, 총 콜레스테롤 등이 음의 상관관계를 가졌다. 폐경 여성의 요추 골밀도와 대퇴골 경부 골밀도는 부위별 근육량 중 몸통과 하체 근육량, 근육량, 제지방량, 기초대사량 등과 양의 상관관계를 보였으며 체지방량, 체지방률, 이완기 혈압에 대해 음의 상관관계를 보였다. 결론: 폐경 전, 폐경 여성에서 요추 및 대퇴골 특히 경부 골밀도는 체 성분 중 제지방량, 근육량, 부위별 근육량, 골격근육량과 양의 상관관계를 가지며, 체지방량, 체지방률, 허리둘레 등 중심비만과 연관 있는 인자들과 음의 상관관계를 보였다. Objectives: We analyzed the relationship between body composition, metabolic parameters, and lumbar and femur bone mineral density (BMD) in pre-and post-menopausal women. Methods: Of 394 females who participated in a medical check-up program, anthropometric measurements and fasting glucose levels and lipid profiles were measured. Body composition analysis was performed using the bioimpedence method and the BMD of the lumbar spine, femur neck, trochanter, ward`s triangle, and total were measured by dual energy X-ray absorptiometry. Results: The mean age was 49.5±9.8 years, and among the subjects, 203 (51.5%) were pre-menopausal and 191 (48.5%) were post-menopausal women. Skeletal muscle mass, fat-free mass, lean body mass, and basal metabolic rate had a positive correlation with the lumbar and femur spine BMD, even after adjustment for age and weight in pre-menopausal women. The abdominal fat ratio, fat mass, waist circumference, percent fat, and total cholesterol had a negative correlation with the lumbar and femur spine BMD, even after adjustment for age and weight in pre-menopausal women. The lean body mass in the legs and trunk, skeletal muscle mass, and fat free mass had a positive correlation with the BMD of the lumbar spine and femur neck, unlike the lean body mass of the arms did not, after adjustment for age and weight in post-menopausal women. Conclusion: In pre- and post-menopausal Korean women, body composition, lean body mass, fat -free mass, and skeletal muscle mass were positive correlates, and fat mass and percent fat were negative correlates with the lumbar spine and femur BMD. (J Korean Soc Menopause 2010;16:29-38)

AspectJ를 지원하는 AOP 개발 프레임워크

박옥자(Oak-Cha Park),김정옥(Jeong-Oak Kim),김재웅(Jae-Woong Kim),유철중(Cheol-Jung Yoo),장옥배(Ok-Bae Chan) 한국정보과학회 2006 한국정보과학회 학술발표논문집 Vol.33 No.1

관심사 분리는 소프트웨어 공학에서 핵심 문제로 다루어왔다. 기존의 OOD나 CBD등은 관심사를 분리하여 모듈화함으로써 프로그램 개발 및 유지보수를 용이하도록 발전해왔다. 하지만, 에러 처리나 로깅과 같이 여러 모듈에 산재되어 실행되는 횡단 관심사는 기존의 방법으로 해결하기 어려웠다. AOP는 이와 같은 횡단 관심사를 처리하려는 데 목적을 두고 제안된 방법으로 기존의 OOD나 CBD의 단점을 보완하면서 병행적으로 발전해왔다. AOP가 나타난 가장 큰 특징은 기존의 개발 방법론을 기반으로 핵심 관심사를 개발하고 해결하기 어려운 횡단 관심사는 AOP로 개발하려는데 초기 목적을 두고 있다. 하지만, 대부분의 연구가 초기 요구사항 분석 단계에서 관심사를 명시하는데 초점을 두고 있을 뿐 구현 단계에서 효율적인 접근 방법은 아직 부족한 편이다. 본 논문에서는 Java와 AspectJ를 이용하여 구현한 간단한 사례 연구를 적용한 AOP 개발 프레임워크를 제안한다. AOP 개발 프레임워크에서는 관심사 분리, 구현, 평가의 세 단계를 기술한다. 이 중 구현단계에서는 핵심 관심사와 횡단 관심사 구현에 초점을 두고 AOP 기법에 쉽게 접근할 수 있는 방법을 기술하고 있다. 프레임워크는 프로그램 개발을 보다 용이하게 하고 확장 및 유지보수시 많은 시간을 단축시키려는데 있다.

Consensus Statements on the Definition, Classification, and Diagnostic Tests for Tinnitus: A Delphi Study Conducted by the Korean Tinnitus Study Group

Choo Oak-Sung,Kim Hantai,Lee Seung Jae,Kim So Young,Lee Kyu-Yup,Lee Ho Yun,Moon In Seok,Seo Jae-Hyun,Rah Yoon Chan,Song Jae-Jun,Nam Eui-Cheol,Park Shi Nae,Song Jae-Jin,Shim Hyun Joon 대한의학회 2024 Journal of Korean medical science Vol.39 No.5

Background: Tinnitus is a bothersome condition associated with various symptoms. However, the mechanisms of tinnitus are still uncertain, and a standardized assessment of the diagnostic criteria for tinnitus is required. We aimed to reach a consensus on diagnosing tinnitus with professional experts by conducting a Delphi study with systematic review of the literature. Methods: Twenty-six experts in managing tinnitus in Korea were recruited, and a two-round modified Delphi study was performed online. The experts evaluated the level of agreement of potential criteria for tinnitus using a scale of 1–9. After the survey, a consensus meeting was held to establish agreement on the results obtained from the Delphi process. Consensus was defined when over 70% of the participants scored 7–9 (agreement) and fewer than 15% scored 1–3 (disagreement). To analyze the responses of the Delphi survey, the content validity ratio and Kendall’s coefficient of concordance were evaluated. Results: Consensus was reached for 22 of the 38 statements. For the definition of tinnitus, 10 out of 17 statements reached consensus, with three statements achieving complete agreement including; 1) Tinnitus is a conscious perception of an auditory sensation in the absence of a corresponding external stimulus, 2) Tinnitus can affect one’s quality of life, and 3) Tinnitus can be associated with hearing disorders including sensorineural hearing loss, vestibular schwannoma, Meniere’s disease, otosclerosis, and others. For the classification of tinnitus, 11 out of 18 statements reached consensus. The participants highly agreed with statements such as; 1) Vascular origin is expected in pulse-synchronous tinnitus, and 2) Tinnitus can be divided into acute or chronic tinnitus. Among three statements on the diagnostic tests for tinnitus only Statement 3, “There are no reliable biomarkers for sensory or emotional factors of tinnitus.” reached consensus. All participants agreed to perform pure-tone audiometry and tinnitus questionnaires, including the Tinnitus Handicap Inventory and Tinnitus Questionnaire. Conclusion: We used a modified Delphi method to establish a consensus-based definition, a classification, and diagnostic tests for tinnitus. The expert panel reached agreement for several statements, with a high level of consensus. This may provide practical information for clinicians in managing tinnitus.

소아 천식 진단과 흡입 스테로이드의 기관지내 직접 효과 판정을 위한 유도 객담의 세포 분석

오재원(Jae Won Oh),고영재(Young Jae Koh),김은아(Eun Ah Kim),이하백(Ha Baik Lee),강정옥(Jung Oak Kang),이상일(Sang Il Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1997 소아알레르기 및 호흡기학회지 Vol.7 No.2

목 적 : 기관지천식은 병리학적으로 염증세포의 기관지내 침착과 그 화학매개체 의해 염증반응이 유발되는 것으로 밝혀져 있으며, 최근 기관지내시경이나 기관지 조직검사, 기관지폐포액 세척 등, 기관지내의 염증에 대해 연구되고 있으나, 이들은 소아에서 침습적인 방법으로 시행상 문제가 있어 왔다. 본 연구는 소아 천식 환아에게 고농도 식염수를 흡입시켜 유도된 객담을 통하여 기관지내의 염증세포들의 분포를 조사하여 소아 천식 진단에 유용한지를 판정하고, 스데로이드의 홉입요법후 기관지내 염증세포의 변화를 측정하고자 하였다. 방 법 : 천식의 설문지 조사을 통해 천식이 있었던 학동기 소아 30명을 선정하고, 고농도 식염수 흡입 유발검사를 통해 현재 천식을 않고 있다고 판정된 소아 16명을 현재 천식군 (FEV_1<85% : PD_15)으로 현재는 천식이 없으나 과거력이 있는 소아 14명을 안정 천식군으로 구분하고, 정상 대조군으로 14명을 선정하여 모두에게 피부검사와 혈청 총 IgE치를 측정하여, 아토피 여부를 판정하였고, 초음파 네뷰라이져을 통해 10분간 4.5% 고농도 식염수를 흡입후 객담을 수집하고, 살부타몰을 1회 흡입시킨 뒤, 현재 천식군과 안정 천식군에게 스테로이드를 2회 흡입후 3시간에 동일한 방법으로 2차 객담을 수집하였다. 결 과 : 아토피 유무는 천식군에서 대조군보다 의미있게 높았으며, 유발검사전 페기능은 정상 대조군이 천식군보다 높았으나, 유의한 차이는 없었다. 유도객담의 총세포수는 각 군에서 많은 차이를 보이지만, 통계적으로 유의한 차이를 보이지는 않았으며, 호산구비는 의미있는 차이를 보였고(현재 천식군 : 7.9±6.0%, 안정 천식군 : 3.8土4.5%, 정상 대조군:0.8±1.4%, P<0.05), 그외 세포들은 차이가 없었으며, FEV_1 predicted %과 유도객담의 호산구비는 유의한 상관 관계는 없었다. 흡입 스데로이드의 기도내 효과를 판정하기 위하여 스테로이드 흡입 3시간후 유도객담의 총 세포수는 현재 천식군, 안정 천식군 모두에서 흡입후 의미있게 감소되었으며, 호산구비도 흡입후 의미있게 차이를 보였다. 결 론 : 호산구비가 각군에서 유의한 차이가 있는 것으로 나타나 소아 천식의 임상 진단에 이용될 수 있을 것으로 판정되고, 양 천식군의 호산구비 차이는 소아 천식의 자연적 호전의 판정에 유용할 것이며, 스테로이드 흡입후 보여지는 호산구비의 감소는 흡입용 스테로이드의 직접적 효과 판정에 유용할 것으로 사료된다. Purpose : Asthma is characterized clinically not only by episodic wheeze and reversible airway obstruction but also by excessive airway secretion. Oppenshaw-Warwick found that 77% of asthmatic subjects reported sputum production as a prominent symptom. Invasive assessment for the airway inflammation in children has been frequently limited. Induced sputum by inhalation of hypertonic saline allows monitoring of airway inflammation in children with asthma in a non-invasive way. Methods : Thirty subjects with asthma were selected by asthma questionares and divided two groups as 14 current symptomatic asthmatics (FEV_1<85%: PD_15) and 16 stable non-symptomatic asthmatics with 4.5% hypertonic saline challenge test, and 14 normal controls were selected. They all inhaled 4.5% hypertonic saline for 10 minutes by ultrasonic nebulizer. The expectorated sputum and saliva were collected from all subjects and collected 3 hours after corticosteroid (Beclomethasone diproprionate 200 ㎍×2) inhalation for asthmatics and were reduced by dithiotreitol. Total cell counts and differentials were determined. Results : Sputum from current asthmatics contained a significantly higher proportion of eosinophil than stable asthmatics or normal controls (7.9±6.0 vs. 3.8±4.5% vs. 0.8±1.4%, P<0.05). The induced sputum after corticosteroid inhalation showed significant decrease in total cell count, eosinophil proportion in current asthma and stable asthma. Conclusion : The cellular analysis of induced sputum was a useful non-invasive and safe study for the diagnosis of childhood asthma and assessment for the direct effects of inhaled corticosteroid on airway inflammation in children with asthma.

점프운동이 내측 비복근 근방추의 형태에 미치는 영향

옥정석,임재형 대한스포츠의학회 1997 대한스포츠의학회지 Vol.15 No.1

The purpose of this study was to evaluate the changes of muscle spindles after jump training. The cross sectional area of muscle spindles was measured by observing light microscopically van Gieson-stained serial sections of muscle of the rat. Intrafusal fibers from the rat medial gestrocnemius were investigated in sedentary animals and animals acutely exercised 50 trials in a day for 10 day for 10 days. Rats were trained in a modified two-way suttle box(7.5W, 0.5mA AC). After a preparatory period of 30 sec from the start of the trial, a light stimulus lasted 5 sec, 5 seconds after the end of the light stimulus, the electric current with 5-duration was applied to the floor grid(foot shock) as the unconditioned stimulus. The electroshock was a series of electro-pulses including both sinusoid and rectangular waves. The rat must jump to the other part of the box in response to the light before the application of the electric shock. The mean cross-sectional area of intrafusal fibers from the exercised animals was significantly less than that of fibers from the sedentary animals(p<0.05). The mean long-radius of intrafusal fibers from the exercised animals was significantly less than that of fibers from the sedentary animals(p<0.01), and so did the mean-circumference of intrafusal fibers. The mean long-radius of nuclear bag fibers from the exercised animals was significantly less than that of fibers from the sedentary animals(p<0.05). The mean cross-sectional area, the mean long-radius, the mean short-radius of nuclear chain fibers from the exercised animals was significantly less than that of fibers from the sedentary animals(p<0.01). On the basis of the results, we have drawn the following conclusions: 1) changes in muscle spindle morphology accompany the jump training, however, The morphology of muscle spindle in the sedentary animals had more levels of the mean cross-sectional area, the mean long-radius, and the mean short-radius than those of fibers in the exercised animals. 2) In contrast to the effect of exercise on the cross-sectional area in extrafusal fibers, there was evidence of less activity in the intrafusal fibers of the exercised animals.

Expression of Mulerian Inhibiting Substance Type II Receptor and Antiproliferative Effects of MIS on Human Cervical Cancer

( Jae Yen Song ),( Mee Ran Kim ),( Young Oak Lew ),( Tae Chul Park ),( Jang Heub Kim ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

Antioxidant, Antimicrobial, and Antitumor Activities of Partially Purified Substance(s) from Green Tea Seed

Jae-Hoon Choi,Jung-Oak Nam,Ji-Yeon Kim,Jin-Min Kim,Hyun-Dong Paik,Chang Han Kim 한국식품과학회 2006 Food Science and Biotechnology Vol.15 No.5

Analysis on STP Strategies of Pyeong Chang Alpensia Resort

Oak-Lan Park,Won-Jae Seo,Seung-JIn Han 한국스포츠과학회 2018 스포츠과학연구(JSAS) Vol.2 No.2

With the rapid increase of tourists for sport participation, sporting resort is becoming popular and a related industry is improving. However, sporting resorts have been faced managerial challenges to survive in the competitive sport tourism markets. One of the key issues is to develop an effective marketing mix for resorts to be suitable for sporting tourists. Hence, the current study analyzed a marketing mix of sporting resort, identified its strategies for segmentation, targeting, and positioning, and finally suggested the effective methods for STP. For the study, PyeongChang Alpensia Resort is selected as a case. The study reviewed the documents and online journals that have covered Alpensia Resort. Via literature review, current marketing mix of Alpensia Resort was identified and its current strategies of STP further were analyzed. For the validity of the results, three experts reviewed the results. Given the decent procedures, firstly, the study suggested that Alpensia Resort needs to seek to develop products that can appeal to MICE industry. Second, Alpensia needs to build all-in-one image by providing products and accommodations including lodging, shopping, ski and related sport programs. Third, Alpensia can seek to enhance marketing mix for inbound sport tourists. Conclusions and implications are further discussed.

Development of breast cancer during postmenopausal hormone therapy in Korea

( Jae Eun Chung ),( Jeong Min Moon ),( Hyun Hee Cho ),( Young Oak Lew ),( Young Taik Lim ),( Jang Heub Kim ),( Jin Hong Kim ),( Mee Ran Kim ) 대한산부인과학회 2009 Journal of Womens Medicine Vol.2 No.2

Objective: The clinicopathologic characteristics of patients with breast cancer arising during postmenopausal hormone therapy were determined. Methods: The study included 29 patients diagnosed with breast cancer during postmenopausal hormone therapy (HT group) and 285 patients diagnosed with breast cancer after menopause who did not receive HT (non-HT group). The data were collected at the three affiliated Hospitals of the Catholic University of Korea over a 10 year period. Tumor size, lymph node metastasis, the presence of estrogen receptors, the stage at the time of diagnosis, the method of treatment, the type of operation, the recurrence rate, and the mortality rate were compared between the two groups. Results: The HT group had smaller tumors than the non-HT group (P=0.0032). In the HT and non-HT groups, 55.2% and 28.6% of the tumors were T1, respectively. In the HT and non-HT groups, 53.9% and 13.4% of the tumors were well differentiated, respectively (P=0.0026). The 5-year survival rate was 100% and 87.6% in the HT and non-HT groups, respectively (P<0.001). Conclusion: Postmenopausal Korean women who developed breast cancer while taking HT had a higher rate of well differentiated tumors, a higher rate of tumors <2 cm in size, and a higher 5-year survival rate than women who developed breast cancer when not taking HT.