흰쥐의 뇌가 성장하는 동안 Ins ( 1 , 4 , 5 ) P3 3 - Kinase 와 mRNA 의 변화

김재웅,최관용,이서구 ( Jae Woong Kim,Kwan Yong Choi,Sue Goo Rhee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5

The enzymic activities of Ins(1,4,5)P₃ 3-Kinase increased by 14-fold during the development of rat brain from fetus to adult. Rat brain contains at least three immunologically distinct IP₃ 3-Kinases(53 kDa, 51kDa and 40kDa). Using molecular probes (monoclonal Ab and cDNA) specific to the IP₃ 3-Kinase, we quantitated the concentration of protein and mRNA of these enzymes during development, these enzymes were qualitatively paralleled by the changes in concentrations of mRNAs. These experiments with IP₃ 3-Kinase indicate that there is only one form of mRNA. At early stage, both IP₃ 3-Kinase protein and mRNA were barely detectible. Their intensities increased suddenly at a week after birth. These results suggest that the concentration of the IP₃ 3-Kinase are regulated at transcriptional level during brain development.

연어알에서 분리한 Carotenoprotein의 구조적 특성

김재웅,민태진,이태녕,Jae-Woong Kim,Tae-Jin Min,Tae-Young Lee 대한화학회 1988 대한화학회지 Vol.32 No.4

Carotenoprotein from Salmo Salar eggs was purified and characterized by CM-cellulose, 50% $(NH_4)_2SO_4$, DEAE-cellulose and sephadex G-75 column. The chromoprotein had a spectrum with ${\lambda}_{max}$ 409, 540 and 580nm in p-buffer (pH 7.0) at initial step. Molecular weights by sephadex G-200 gel filtration were 50, 200 and 26,000 daltons. SDS-PAGE analysis showed a structure with four identical subunits (12,500 daltons). Its sample retained a small amount of carbohydrates and lipids. Amino acids were analyzed, and mannose, galactose and glucosamine also were identified. Carotenoid extacted with acetone was found to be astaxanthin ester by partition test, epoxy test, iodine test, allylic test, reduction, acetylation, uv/vis, ir and nmr datas. Stearate (47.9%) and palmitate (21.4%) were predominant fatty acids in the astaxanthin ester. 연어알(Salmo Salar)에서 carotenoprotein을 CM-세룰로스, 50% 황산암모늄 침전, DEAE-세룰로스 및 세파덱스 G-75젤을 사용하여 충분히 정제하였다. 인산염 완충용액(pH 7.0)에서 자외선 흡수극대는 ${\lambda}_{max}$ 409, 540 그리고 580nm이었다. 세파덱스 G-200젤 여과법으로 측정된 분자량은 50, 200rhk 26,000달톤으로 heterogeneous한 두가지 형태였다. 전자는 적자색으로 SDS-PAG 전기영동 결과 분자량이 12,500달톤인 4개의 동일한 subunit로 구성되었고, 상당량의 지질과 만노오스, 갈락토스와 글루코사민을 함유하고 있음을 밝혔다. carotenoprotein의 색소를 추출하여 tlc로 정제한 다음 분배비 시험, 요오드 시험, 에폭시 그룹 시험, 알릴릭 시험, 환원 시험 그리고 아세틸화 시험등의 유기반응과 uv/vis, ir 및 nmr 데이타를 얻어 구조를 결정한 결과 carotenoid는 astaxanthin ester임을 확인하였으며 그 ester는 스테아르산(47.9%)과 팔미트산 (21.4%)이 대부분이었다.

Purification and Characterization of Carboxypeptidase B from Wild Salmon (Salmo Salar) Eggs

김재웅,민태진,Jae Woong Kim,Tae Jin Min Korean Chemical Society 1985 대한화학회지 Vol.29 No.3

연어(Salmo Salar)알 중의 Carboxypeptidase B를 CM-셀룰로오스, 0.5포화황산 암모늄, DEAE-셀룰로오스 및 세파덱스 G-75젤로 정제하여 그 성질을 조사하였다. 이 효소의 최적 온도는 55$^{\circ}C$였으며, 최적 pH는 4.0과 7.0이었고, pH안정성은 2.0∼3.0 및 5.5∼7.0이었다. 히푸릴-L-아르기닌 기질에 대하여 글리실-L-아르기닌 부위를 절단하는 특이성을 보였고, 그 K$_m$값은 0.21mM이었다. Cu$^{2+}$ 와 Fe$^{3+}$ 존재하에서는 효소의 활성도가 증가하였지만 Zn$^{2+}$의 경우에는 감소하였다. 특히 리신은 히푸릴-L-아르기닌 기질에 대하여 경쟁적 억제작용을 보였으며, K$_i$값은 4.3mM이었다. 분자량은 36,400돌톤이었고, 19종류의 아미노산으로 구성된 단위체이었다. Carboxypeptidase B from Salmo Salar eggs was purified by CM-cellulose, 0.5 ammonium sulfate saturation, DEAE-cellulose, and Sephadex G-75 gel filtration and its enzymatic properties were investigated. Optimum temperature was 55$^{\circ}C$, pH optima were 4.0 and 7.0 at 37$^{\circ}C$, and the enzyme was stable at pH 2.0∼3.0 and 5.5∼7.0 for 1.5h. This enzyme showed substrate specificity hydrolyzing the peptide bond of glycyl-L-arginine. Its K$_m$ values was 0.21mM, and the enzyme activity was stimulated by Cu$^{2+}$ and Fe$^{3+}$, while inhibited by Zn$^{2+}$. The lysine was found to be competitive inhibitor and its K$_i$ value was determined to be 4.3mM. Molecular weight of this enzyme was determined to be 36,400 daltons by SDS-PAGE and the enzyme was monomeric protein composed of 19 kinds of amino acid residues.

용액 전구체의 닥터블레이드 코팅 및 셀렌화 열처리를 통한 CuInSe₂ 박막 태양전지용 광흡수층 제조

김재웅,안세진,윤재호,이정철,윤경훈,Kim, Chae-Woong,Ahn, Se-Jin,Yun, Jae-Ho,Lee, Jeong-Chul,Yoon, Kyung-Hoon 한국재료학회 2008 한국재료학회지 Vol.18 No.6

In this paper, a novel non-vacuum technique is described for the fabrication of a $CuInSe_2$ (CIS) absorber layer for thin film solar cells using a low-cost precursor solution. A solution containing Cu- and Inrelated chemicals was coated onto a Mo/glass substrate using the Doctor blade method and the precursor layer was then selenized in an evaporation chamber. The precursor layer was found to be composed of CuCl crystals and amorphous In compound, which were completely converted to chalcopyrite CIS phase by the selenization process. Morphological, crystallographic and compositional analyses were performed at each step of the fabrication process by SEM, XRD and EDS, respectively.

사철느타리버섯 중 $G_{418}$-sensitive 미토콘드리아성 ATPase/ATP synthase의 특성

김재웅,김동희,이정복,이서구,민태진,Kim, Jae-Woong,Kim, Dong-Hee,Lee, Jung-Bock,Lee, Sur-Koo,Min, Tae-Jin 한국분석과학회 1992 분석과학 Vol.5 No.4

형질 전환된 사철느타리버섯으로부터 초원심분리 및 설탕밀도 기울기법으로 44% 층에서 미토콘드리아를 분리정제하였다. ATPase와 ATP synthase의 최적 활성조건은 각각 pH 7.4, $60^{\circ}C$ 및 pH 7.5, $57^{\circ}C$였고, km값은 11.6mM과 8.4mM였다. ATPase는 기질농도 5~6mM의 ATP에서, ATP synthase는 5~10mM ADP 농도에서 활성이 높으며, 그 이상의 농도에서는 기질 저해를 받았다. ATPase/ATP synthase 모두 $Mg^{2+}$ 의존성 효소로 $G_{418}$으로 비경쟁적인 저해를 받았다. 효소의 아미노산 분석결과 hydrophobic 아미노산 잔기는 50.5%, small 아미노산 잔기는 56.1%, hydrogen bonding 아미노산 잔기는 43.7%, helix breaking 아미노산 잔기는 55.2%였다. 인지질을 분석한 결과 phosphatidyl glycerol, phosphatidyl choline 및 phosphatidyl ethanolamine으로 구성되었고 phosphatidyl serine과 phosphatidyl inositol은 전혀 없었다. 포화 지방산은 palmitate(51.31%)와 stearate(18.32%)의 함량이 많았고, 불포화 지방산($C_{18:1}$, $C_{18:2}$ 및 $C_{16:1}$)의 함량도 많았다. The mitochondrion was purified at 44% sucrose layer from pleurotus florida by using ultracentrifuge and sucrose density gradient method. Optimum pH and temperature of ATPase and ATP synthase were pH 7.4, $60^{\circ}C$ and pH 7.5, $57^{\circ}C$ respectively, also their Km values were determined as 11.6mM and 8.4mM. ATPase was activated at 5~6mM ATP substrate concentration, then ATP synthase was 5~10mM range ADP. ATPase/ATP synthase were $Mg^{2+}$-dependent enzyme, partially inhibited by their substrate, and then showed an none competitive inhibition pattern by $G_{418}$. Amino acid composition of ATPase/ATP synthase was as follows, hydrophobic amino acid residue was 50.5%, small residue, 56.1%, hydrogen bonding residue, 43.7% and helix breaking residue, 55.2%. Phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl glycerol were contained but not phosphatidyl inositol and phosphatidyl serine. Palmitate(51.31%), stearate(18.32%) and unsaturated fatty acids($C_{18:1}$, $C_{18:2}$ and $C_{16:1}$) were predominated.

다양한 임상양상을 보인 영유아기 포도상구균성폐렴 5례

김재웅,김일경,성호,최창희,Kim, Jae Woong,Kim, Il Kyung,Sung, Ho,Choi, Chang Hee 대한소아감염학회 1998 Pediatric Infection and Vaccine Vol.5 No.2

Staphylococcal pneumonia caused by staphylococcus aureus can be characterized by its severity and rapid progress as a bacterial infection. The disease shows a high mortality in younger patients, especially in infants unless early and appropriate treatment is carried out. Treatment can be made of medical method alone but in cases of surgical interventions are needed, immediate surgical methods such as closed or open drainage of pleural fluid, lobectomy and decortication should be followed with combination of medical therapy. The choice of antibiotic should be made by proper antibiotic sensitivities tests. For a methicillin sensitive S. areus(MSSA), the penicillase resistant penicillin would be the first choice and for a methicillin resistant S. aureus (MRSA), the glycopeptides such as vancomycin would be the first one. Other drugs can also be used if the bacterial agents show any sensitivities to these drugs. Commonly, the chest roentgenographic findings reveal infiltrations, empyema, pneumothorax, pleural effusion, atelectasis or pneumatoceles in staphylococcal pneumonia and this fact easily can lead the physicians to its diagnosis as soon as possible. We experienced 5 cases of staphylococcal pneumonia in infants, proven by through bacterial cultures and report them with brief review of the related literatures.

Developmental Changes of Ins(1,4,5)$P_3$ 3-Kinase and mRNA in Rat Brain

김재웅,최관용,이서구,Kim, Jae-Woong,Choi, Kwan-Yong,Rhee, Sue-Goo 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.5

Rat brain이 성장하는 동안 Ins(1,4,5)$P_3$ 3-Kinase의 활성도가 14배 증가하였다. 면역학적으로 서로 다른 $IP_3$ 3-Kinase가 적어도 3가지 있었다(53 kDa, 51 kDa 및 40 kDa). $IP_3$ 3-Kinase에 특이성이 있는 모노클로날 항체와 cDNA를 probe로 사용하여 단백질과 mRNA의 양을 정량하였다. 한 가지 형태의 mRNA가 확인되었고, Parallel하며, 어린단계에서는 $IP_3$ 3-Kinase와 mRNA가 전혀 나타나지 않았으나 1주일 후에는 갑자기 증가하였다. 실험결과 흰쥐의 뇌가 성장하는 동안 $IP_3$ 3-Kinase는 transcriptional 수준으로 조절 받는 것이 확인되었다. The enzymic activities of Ins(1,4,5)$P_3$ 3-Kinase increased by 14-fold during the development of rat brain from fetus to adult. Rat brain contains at least three immunologically distinct $IP_3$ 3-Kinases(53 kDa, 51kDa and 40kDa). Using molecular probes (monoclonal Ab and cDNA) specific to the $IP_3$ 3-Kinase, we quantitated the concentration of protein and mRNA of these enzymes during development, these enzymes were qualitatively paralleled by the changes in concentrations of mRNAs. These experiments with $IP_3$ 3-Kinase indicate that there is only one form of mRNA. At early stage, both $IP_3$ 3-Kinase protein and mRNA were barely detectible. Their intensities increased suddenly at a week after birth. These results suggest that the concentration of the $IP_3$ 3-Kinase are regulated at transcriptional level during brain development.

태권도 돌려차기 시 기술 수준에 따른 몸통, 골반, 지지 다리의 운동학적 변인 비교

김재웅 ( Kim Jae-woong ),한기훈 ( Han Ki-hoon ),김제민 ( Kim Je-min ),이상우 ( Lee Sang-woo ),권문석 ( Kwon Moon-seok ),터틀노엘 ( Noelle J. Tuttle ) 국기원 2018 국기원태권도연구 Vol.9 No.1

이 연구의 목적은 태권도 돌려차기 시 기술 수준에 따른 몸통, 골반, 지지 다리의 운동학적 변인을 비교 분석하는 데 있다. 본 연구를 위해 블랙 벨트 소지자로서 기술 수준에 따라 중급자10명, 상급자 9명이 실험에 참여하였다. 3차원 동작 분석 시스템을 사용하여 골반, 몸통, 지지다리의 방향각을 산출하였다. Start, Toeoff, MKF, Max, Impact, Min에서 그룹 간의 평균 방향각을 비교하기 위하여 독립표본 t-검정을 하였다. MKF, Impact, Min에서 골반의 좌측 기울어짐 각도가 그룹 간의 유의한 차이가 나타났다. Start와 Max에서 지지 다리의 엉덩관절 굴곡 각도와 MKF에서 지지 다리의 엉덩관절 외전 각도 사이에 유의한 차이가 있었다. 이상의 결과를 볼 때, 상급자들은 푸쉬단계에서 엉덩관절의 굴곡 각도가 증가 되었고, MKF에서는 외전 각도가 감소하였다. 또한, 스트라이크 단계에서 골반의 좌측 기울어짐 각도는 감소하였다. The study purpose was to investigate skill level differences on trunk, pelvis, and supporting leg kinematics in a Taekwondo roundhouse kick. 10 intermediate and nine expert male black-belt holders executed roundhouse kicks at a target. Orientation angles of the pelvis, trunk, and supporting -thigh, -shank, and -foot were obtained through a three-dimensional video motion analysis. Orientation angles at Start, Toeoff, Maximum Knee Flexion(MKF), Impact, Maximum angle(Max), and Minimum angle(Min) were compared between groups using independent t-tests(p<.05). Significant differences between groups were found in the pelvis left lateral tilted position at MKF, Impact, and Min; significant differences were also found in the supporting hip joint flexed position at Start and Max, and abducted position at MKF. It was concluded that higher performers increased the hip joint flexion angle in the Push phase, decreased abduction angle at MKF, and decreased the pelvis left tilted angle in the Strike phase.

Uterine tumor resembling ovarian sex - cord tumor 1 예와 그 임상적 고찰

김재웅(Jae Woong Kim),한은미(Eun Mee Han) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.2

Uterine tumor resembling ovarian sex-cord tumor (UTROSCT) were first described by Clemen and Scully in 1976. They are a heterogenous group of uterine neoplasm characterised by pure or predominant histological patterns that resembled sex cord tumors of ovary. Clemen and Scully divide the tumors into two groups according to sex-cord-like structures within tumors (the so-called group I tumors with 50% of sex-cord elements, the so-called group II tumors with almost completely sex-cord-like structures). The histogensis of the sex-cord-like elements is uncertain, the group I tumor appears to be more malignant. We experienced a case of UTROSCT in a 44 years old woman underwent LAVH under tantative diagnosis of uterine myoma and report the case with brief review of literature.

애니메이션의 역사적 양식에 대한 연구

김재웅(Kim Jae-Woong) 한국만화애니메이션학회 2009 만화애니메이션연구 Vol.- No.16

이 논문에서는 애니메이션의 양식에 대한 유형을 분석하였다. 역사적 양식에는 자기 자신만의 고유한 특정기질을 드러내는 개인양식과 유사한 환경을 공유하는 보편적 성향인 시대양식, 그리고 집단의 형태적 취향과 민족정서를 드러내는 민족양식으로 분류할 수 있다. 이리 트른카와 팀 버틑, 유리 놀스테인 등의 개인양식은 각 개인의 독특한 감수성과 관찰력이 뛰어난 영상미로 표현하고 있으며, 주제에 대한 해석과 매체를 다루는 기술이 뛰어남을 알 수 있다. 애니메이션의 고전적 규범의 신기원을 구축한 디즈니는 풍부한 표정언어와 섬세한 동세의 풀 애니메이션으로 미국애니메이션의 민족양식을 완성시켰고, 일본 아니메의 역사양식에는 몇 몇 감독들의 노력과 함께 휴머니즘적 사상, 그리고 일본의 사회문화와 전통의 맥을 이어가는 지브리가 그 중심에 있었다. I try to extract typological factors from the historically relevant works of animation, for that we can refer 'style.' creator's individuality, general tendencies and restraints of his time, the national properties work on them. It is the individual aspect that excels in the works of Jiri Trnka, Tim Burton, Yuri Norstein, so they stand out not only in their own specific sensibility, vision but also in the elucidation of themes and the technology of dealing medium. On the other hand the Walt Disney's animation has so distinctive characteristics that we could identify them. Disney's so-called classical model accomplished a typically American animation form as full of expressive visual language, over reaction, full animation describing detail action. We could tell, Miyajaki Hayao's Gibri Studio represents Japanese animation, which works on traditional motives of Japan and expresses unique humanity.