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Mycoplasma pulmonis의 LAMP를 이용한 ELISA 법 개발
김동재(Dong-Jae Kim),박종환(Jong-Hwan Park),석승혁(Seung-Hyeok Seok),조선아(Sun-A Cho),백민원(Min-Won Baek),이희영(Hui-Young Lee),장동덕(Dong Deuk Jang),양기화(Ki-Hwa Yang),한범석(Beom-Seok Han),박재학(Jae-Hak Park) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.3
Mycoplasma pulmonis infection causes chronic respiratory disease and decreases reproductive efficiency. Infected mice display inactivity, weight loss, and ruffled hair coat, but the most prominent signs are "chattering" and dyspnea, due to rhinitis and purulent exudate in nasal passages. In this study we developed the ELISA method for M. pulmonis using LAMP (Lipid-Associated Membrane Protein) of Mycoplasma pulmonis. We isolated M. pulmonis from bronchial flushing fluid of conventionally housed mice and rats. The LAMP of isolated M. pulmonis is used in ELISA antigen development The results of serological test, in the ELISA with LAMP of M. pulmonis, were equal to those of a commercial ELISA kit. Our ELISA method will be used to eradicate Mycoplasma pulmonis infection in laboratory animal facilities and contribute to quality control of laboratory animals.
간과 선장의 암유발과정에서 혈액화학효소 및 DNA ploidy pattern 의 변화에 대한 조사
정자영,장동덕,조재천,이영순,Jeong, Ja-Young,Jang, Dong-Deuk,Cho, Jae-Cheon,Lee, Yong-Soon 한국수의병리학회 1998 한국수의병리학회지 Vol.2 No.2
This study was carried out to investigate on the serum chemistry and the DNA ploidy changes in carcinogenesis of the rat liver and kidney. Sixty male Sprague-Dawley rats were divided into two groups. Group I was non-treated control. Group II was given initiators (2,2'-dihydroxy- di-N-propylnitrosamine, 0.1% in drinking water(d.w.) for 1 week and N-ethyl-N-hydroxy-ethylnitrosamine; 0.15% in d.w. for 1 week) and promoters (3'methyl-cholanthrene; 3'MC, l0mg/kg, intraperitoneally(i.p.) twice a week and DL-serine; 0.05% in d.w. for 5 weeks, from 3 to 8 weeks). All examinations were performed at 12 and 20 weeks RBC, HGBCp<0.05) and PCVCp<0.01) significantly decreased in Group II at 20 weeks. Activities of ALT, AST(p<0.05) and GGT(p<0.01) were significantly increased in Group II at 20 weeks. Flow cytometric analysis showed hepatocyte nuclei from normal livers were predominantly tetraploid(66~67%) and then diploid(28~30%). Most of hepatocyte nuclei from carcinogen-treated rats were diploid (52~68%) and less were tetraploid(28~42%). Neoplastic liver nodules and hepatocellular carcinoma contained almost exclusively diploid nuclei. Renal cell nuclei from normal kidney were predominantly diploid(88~93%), those from carcinogen-treated rats had an abnormal DNA-content peak(aneuploidy, 6-7%), near the tetraploidy area. These results suggest that diploidy may be an effective screening marker of the liver carcinogenesis. Aneuploidy may be an useful marker in assessment of the experimental renal carcinogenesis.
Cyclopiazonic acid 및 aflatoxin B<sub>1</sub>이 토끼의 혈소판에서 arachidonic acid 대사, 칼슘 동원 및 초미세구조에 미치는 영향
홍충만,장동덕,조명행,Hong, Choong-man,Jang, Dong-deuk,Cho, Myung-haing 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
For better understanding the interrelationship of hemorrhage and aggregation mechanism, cyclopiazonic acid(CPA) known as promoting the aggregation of platelet, aflatoxin $B_1(AFB_1)$ inhibiting platelet aggregation were used as toxic mycotoxins in these studies. In order to investigate the potential role of prostaglandin metabolism on the platelet aggregation, a variety of prostaglandin metabolites such as $PGF_{2{\alpha}}$, $PGE_2$ and $TXB_2$ were measured in homogenized rabbit platelets by TLC and LSC. And the role of $Ca^{{+}{+}}$ on the platelet aggregation was investigated by flow cytometer. Finally, the morphological effects of mycotoxins on platelet were determined by transmission electron microscope. The results and conclusions obtained from these studies are: 1) CPA induced no changes but $AFB_1$ increased $PGE_2$ and $TXB_2$. 2) CPA promoted ADP, collagen, thrombin, A.A., and PAF-induced $Ca^{{+}{+}}$ release. $AFB_1$, however, decreased $Ca^{{+}{+}}$ level except collagen-induced $Ca^{{+}{+}}$ release. When the calcium blocker, verapamil, was used, CPA decreased thrombin-induced $Ca^{{+}{+}}$ release and increased collagen, ADP, PAF and A.A.-induced $Ca^{{+}{+}}$ release. $AFB_1$ in contrast decreased the all factors induced $Ca^{{+}{+}}$ release. 3) $AFB_1$ did not induce any ultrastructural changes except large vacuole formation in a few platelets. And CPA also did not induce any changes except moderate shape change, indicator of platelet activation. In conclusion, CPA promoted platelet aggregation by the increases of $Ca^{{+}{+}}$ release but had no changes in A.A. metabolites. Antiaggregating effects of $AFB_1$ may be due to decreases of $Ca^{{+}{+}}$ release and increases of $PGE_2$ and $PGF_{2{\alpha}}$ formation. These data provide the basis for the future study of mobilization and function of $Ca^{{+}{+}}$ in platelet aggregation.
Clofibrate 의 유도체가 토기의 혈소판 응집에 미치는 영향
홍충만(Choon Man Hong),장동덕(Dong Deuk Jang),신동환(Dong Hwan Shin),조재천(Jae Chon Cho),조명행(Myung Haeng Cho) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.2
Several clofibrate congeners (bezafibrate, gemfibrozil and fenofibrate) were investigated the relationship between effects on the aggregation induced by aggregating agents (thrombin, arachidonic acid, ADP and collagen) and arachidonic acid metabolism in rabbit homogenized platelet. In platelet aggregation study, all drugs produced no significant inhibition (data not shown) in arachidonic acid and thrombin. Also platelet aggregation by ADP was not changed in bezafibrate and inhibited dose dependently in fenofibrate and gemfibrozil. Platelet aggregation by collagen was inhibited dose dependently and significantly (from p<0.5 to p<0.001) by gemfibrozil and fenofibrate at concentrations between 20 and 400 μM. In arachidonic acid metabolism study, synthesis of thromboxane B₂ was not changed in rabbit platelet membranes and that of prostaglandin E₂ and F_(2α) was slightly increased by all drugs. It was concluded that clofibrate congeners inhibited ADP and collagen induced rabbit platelet aggregation and inhibition of collagen induced aggregation was probably mediated through some mechanism (pathway) other than arachidonic acid metabolism, judging from arachidonic acid metabolites (thromboxane B₂, prostaglandin E₂ and F_(2α)) synthesis in rabbit homogenized platelet.
생약 복용에 따른 아플라톡신 B<sub>1</sub>의 인체모니터링 연구
이진희,류희영,김현경,김도정,이영주,정수희,장동덕,김형수,홍연표,윤혜성,Lee, Jin-Hee,Ryu, Heui-Young,Kim, Hyun-Kyung,Kim, Do-Jung,Lee, Young-Joo,Jung, Su-Hee,Jang, Dong-Deuk,Kim, Hyung-Su,Hong, Yeon-Pyo,Yoon, Hae-Seong 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.2
Aflatoxin $B_1$, a known human carcinogen, is the member of aflatoxin subfamily that is most frequently found in contaminated foods. Epidemiological studies have suggested that aflatoxins may be associated with human liver cancer and acute hepatitis. Recently it was reported that the traditional medical herbs sold in domestic markets are contaminated with aflatoxins. Long-term administration of these contaminated medicines could result in adverse health effects. Therefore, it is important to evaluate the levels of exposure to aflatoxin in people who ingest traditional herbal medicines. Blood samples were collected, before and after the herbal medicine intake, from 151 subjects who visited the hospital. The metabolite of aflatoxin $B_1$ in blood, aflatoxin $B_1$-albumin (aflatoxin $B_1$-lysine), is reportedly an appropriate internal exposure indicator, and its levels in the collected bloods were therefore analyzed using a liquid chromatography-mass spectrometry. The analytical method of aflatoxin $B_1$-lysine in blood was firstly optimized in Korea and the levels were detected below quantification limits (2 pg/mg albumin) in this study population. Consequently, the exposure levels of aflatoxin $B_1$ by ingestion of herbal medicines were low but it is important to monitor routinely due to the possibility of risk on the aflatoxin exposure.
Indole-3-carbinol(I3C) 투여에 의한 7,12-Dimethylbenz[α] anthracene(DMBA) 유발 랫드 유선발암 억제
강진석,안병우,남기택,최미나,김지영,김대중,장동덕,양기화,Kang, Jin Seok,Ahn, Byeongwoo,Nam, Ki Taek,Choi, Mina,Kim, Ji Young,Kim, Dae Joong,Jang, Dong Deuk,Yang, Ki-Hwa 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4
Indole-3-carbinol (I3C), one component of cruciferous vegetables (the Fammily of Cruciferae), has been shown to exert its chemopreventive effect in liver, colon and mammary tissue before or concurrent exposure of carcinogen, but there have been several evidences that consumption of I3C induced tumor promotion in some tissues. Our studies were investigated to examine the modifying effects of I3C in the 7,12-dimethylbenz[$\alpha$]anthracene (DMBA) induced rat mammary gland tumor model. Fifty-two female Sprague-Dawley rats were randomly divided into five groups. Animals of the group 1 were given the diet containing 100ppm I3C and animals of the groups 2 and 4 were given the diet containing 300ppm I3C from 6 weeks of age. At 7 weeks of age, the animals of the groups 1, 2 and 3 were intubated with DMBA. All amimals were killed at 20 weeks after carcinogen treatment. There were significant increases of food consumption in I3C feeding groups compared with those of basal diet feeding groups. The incidences of the mammary tumors in the group 1, 2 and 3 were 75.0% (9/12), 56.3% (9/16) and 93.8% (15/16), respectively and the average number of tumors of group 1 (DMBA+I3C 100ppm: $2.08{\pm}0.61$) and 2 (DMBA+I3C 300ppm: $1.19{\pm}0.32$) were significantly lower than that of group 3 (DMBA alone: $4.63{\pm}0.72$) at the value of P<0.05 and P<0.001, respectively. In the pathological examination of appearing tumors, most of them were adenocarcinoma. Many epithelial cells of tumors showed strong estrogen receptor (ER) $\alpha$ expression but there were slight difference of ER $\alpha$ expression among the type of tumors. We suggest that pre-initiation treatment of I3C has an inhibitory effects on mammary carcinogenesis induced by DMBA.
청목향 Aristolochiae radix에 있어 F344 랫드의 독성
김충용,김용범,양병철,이종화,정문구,양기화,장동덕,한상섭,강부현,Kim, Choong-Yong,Kim, Yong-Bum,Yang, Byung-Chul,Lee, Jong-Hwa,Chung, Moon-Koo,Yang, Ki-Hwa,Jang, Dong-Deuk,Han, Sang-Seop,Kang, Boo-Hyon 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.1
13-week orally repeated dose toxicity was investigated to ascertain the toxic effects of Aristolochiae radix in F344 rats at dose levels of 0, 1 (0.003 AA, aristolochic acid, mg/kg), 5 (0.014 AA mg/kg), 25 (0.068 AA mg/kg), 125 (0.34 AA mg/kg), and 500mg/kg (AA 1.36 mg/kg). No mortalities were found in any of the dose groups including vehicle control groups of both sexes during the study period. Hematologic and serum biochemical examinations revealed no changes related to the test item in any of the dose groups of both sexes. However, gross findings at necropsy implicated thickening of the stomach wall. In histopathological examinations, prominent findings related to the test item treatment were observed in the stomach and urinary bladder. There were squamous cell papilloma, squamous cell hyperplasia, ulceration and erosion observed in the non-glandular stomach. Squamouse cell hyperplasia was observed at dose levels of more than 125 mg/kg in both sexes and squamous cell papilloma was observed at dose level of 500 mg/kg in both sexes. The incidence and severity of these proliferating lesions including squamous cell hyperplasia and squamous cell papilloma increased with dose dependency. Transitional cell hyperplasia was also observed in the urinary bladder at dose levels of more than 25 mg/kg in both sexes and the incidence and severity of the lesion increased with dose dependency. In conclusion, the toxic changes related to the test item treatment were observed in the stomach and urinary bladder, and the no-observed-adverse-effect level (NOAEL) was estimated to be 5 mg/kg/day for both males and females in F344 rats.