위의 저등급과 고등급 이형성에서 면역조직화학염색을 이용한 telomerase 발현의 비교

오영상 ( Young Sang Oh ),김호동 ( Ho Dong Kim ),문승원 ( Seung Won Moon ),정종혁 ( Jong Hyeok Jeong ),김동한 ( Dong Han Kim ),양혁승 ( Hyuk Seung Yang ),김수현 ( Soo Hyun Kim ),김상필 ( Sang Pil Kim ),전원정 ( Won Jeong Jeon ) 대한내과학회 2007 대한내과학회지 Vol.72 No.4

목적: Telomere는 세포 염색체의 말단에 존재하며 염색체의 안정을 유지하는데 필수적인 역할을 수행한다. 이러한 telomere의 합성을 촉매하는 telomerase 활성이 전암 병변에서도 관찰되면서 이 효소의 활성화는 발암과정의 초기 현상으로 암화과정에 기여하는 것으로 생각되고 있다. 전암성 병변으로 알려진 이형성의 단계에 따른 telomerase의 발현 정도를 측정하고 연관관계를 밝히고자 하였다. 방법: 현재까지 본원에서 위내시경을 시행한 환자들 중 병리조직검사에서 위의 저등급 이형성과 고등급 이형성으로 확진된 환자 43명을 대상으로 하여 후향적으로 연구하였다. 조직을 포르말린에 고정한 후 제작된 환자의 파라핀 조직 절편을 4 ㎛ 두께로 자른 다음 슬라이드에 부착하여 충분히 건조시켰고, 탈파라핀과 함수과정을 거친 조직 절편은 과산화수소에 반응시켰다. 슬라이드를 버퍼(buffer)에 넣고 압력기구에서 끓인 후 실온에서 방치하였으며, PBS로 세척한 다음 blocking solution으로 전처치 하였다. 일차 항체를 희석하여 4℃에서 하룻밤 동안 반응시켰고, 이차항체를 각각 반응시킨 후 세척하고 발색하여 대조 염색하였다. 광학현미경에서 면역조직화학염색에 양성반응을 보이는 이형성 세포의 비율을 25% 미만, 25~50%, 51~75%, 76% 이상으로 나누어 다섯 등급(0, 1+, 2+, 3+, 4+)으로 나타내었다. 염색은 세포의 핵에 국한되어야 하며, 두 군 간의 구분 기준은 세포의 25% 이하가 염색된 경우와 26% 이상이 염색된 경우로 하였다. 결과: 저등급 이형성 22명 중에는 남자 13명, 여자 9명이었으며 고등급 이형성 21명 중에는 남자 14명, 여자 7명이었다. 연령은 저등급 이형성이 64.4±9.0세였고, 고등급 이형성이 69.2±9.6세였다. 두 군 간의 성별과 연령은 무관하였다. 면역조직화학염색에서 25% 이하로 염색된 조직은 저등급 21명, 고등급 5명이었으며 26%이상 염색된 조직은 저등급 1명, 고등급 16명으로 저등급과 고등급 간에 telomerase 발현 정도의 유의한 차이가 있었다. 결론: 위의 이형성에서 저등급에서 고등급으로 갈수록 telomerase 발현 정도가 높아짐을 알 수 있었다. Background: Telomeres are simple repeats elements located at each end of the chromosomes of eukaryotic cells. The main function of telomeres is to cap the chromosome end and protect it from enzymatic attack. Telomerase that facilitates the synthesis of telomere has been detected in not only cancer, but also in precancerous lesion. In this study, we compared the telomerase expression between low-grade and high-grade gastric dysplasia. Methods: The telomerase expression of 43 patients with gastric dysplasia (22 low-grade and 21 high-grade) was evaluated by immunohistochemical staining in tissues. Results: The telomerase expression was much higher in the tissues from the patients with high-grade gastric dysplasia than in those tissues of the patients with low-grade gastric dysplasia. Conclusions: Activation of telomerase may be related with the malignant potentiality in gastric cells. Further studies are needed to define the role of telomerase in gastric tumorigenesis. (Korean J Med 72:368-375, 2007)

혀 세정기가 부착된 수동칫솔이 치아와 혀의 세정도 및 구취 관리도에 미치는 영향

김태일,홍삼표,이신재,강봉선,오영상,김인경,오미현,Kim, Tae-Il,Hong, Sam-Pyo,Lee, Shin-Jae,Kang, Bong-Sun,Oh, Young-Sang,Kim, In-Kyung,Oh, Mi-Hyun 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.4

Purpose: The aim of this study was to investigate the effect of tongue cleaner-equipped manual toothbrush on tooth cleanness, tongue cleanness and malodor index. Materials and methods: 504 subjects were included in this study. At 1st visit, basic information such as age, sex, smoking amount and alcohol consumption was recorded. Self assessment by individual subjects was performed regarding satisfaction to old toothbrush and toothbrushing habit. Tooth cleanness, tongue cleanness and malodor index was assessed by professional researcher. Tongue cleaner-equipped manual toothbrush was given to each subject with proper toothbrushing instruction. After 1 month passed, self assessment and researcher assessment regarding the same index were performed and analyzed statistically by chi-square test. Results: At 1st visit subjects seem to ignore tongue cleansing and showed poor tooth cleanness index, tongue cleanness index and malodor index, however the same subjects were motivated to clean their tongue and teeth and presented statistically improved distribution pattern in tooth cleanness index, tongue cleanness index and malodor index after using tongue cleaner-equipped manual toothbrush(p<0.01). Satisfaction to tongue cleaner-equipped manual toothbrush was 98%. Conclusion: Tongue cleaner-equipped manual toothbrush would be an effective tool for maintaining good oral hygiene through improving tooth and tongue cleanness and preventing malodor formation.

대장 관샘종에서 면역조직화학염색을 이용한 Telomerase 발현

김호동 ( Ho Dong Kim ),오영상 ( Young Sang Oh ),김수현 ( Soo Hyun Kim ),김상필 ( Sang Pil Kim ),신현학 ( Hyun Hak Shin ),박주용 ( Ju Yong Park ),박혁 ( Hyeuk Park ),명보현 ( Bo Hyun Myoung ),김도현 ( Do Hyun Kim ),이영직 ( Young 대한소화기학회 2007 대한소화기학회지 Vol.50 No.3

목적: Telomere는 세포 염색체의 말단에 존재하며 염색체 안정을 유지하는 데 필수적인 역할을 수행한다. 이러한 telomere의 합성을 촉매하는 telomerase 활성이 전암 병변에서도 관찰되면서 이 효소의 활성화는 발암과정의 초기 현상으로 암화과정에 기여하는 것으로 생각하고 있다. 대장암의 전암 병변으로 알려진 대장 관샘종의 형성이상 단계에 따른 telomerase의 활성도를 측정하고 연관관계를 밝히고자 하였다. 대상 및 방법: 본원에셔 2006년 1윌부터 2006년 12윌까지 대장내시경 용종절제술 또는 수술 받은 환자들 중 병리 조직검사에셔 경도 형성이상과 고도 형성이상으로 확진한 환자 48예를 대상으로 하여 후향적으로 연구하였다. 광학현미경에서 면역조직화학염색에 양성반응을 보이는 형성이상 세포의 비율이 50% 이하로 염색된 human telomerase reverse transcriptase (hTERT) 저염색군과 51% 이상 염색된 hTERT 고염색군으로 나타내었다. 염색은 세포의 핵에 국한된 경우로 하였다. 결과: 면역조직화학염색에서 hTERT 저염색군은 경도 형성이상 22예(96%), 고도 형성이상 13예(52%)이었으며 hTERT 고염색군은 경도 형성이상 1예(4%), 고도 형성이상 12예(48%)로 경도 형성이상과 고도 형성이상 간에 telomerase 활성도의 유의한 차이가 있었다(p<0.05). 결론: 대장 관샘종의 형성이상이 경도에셔 고도로 갈수록 telomerase 활성도가 높아졌다. Background/Aims: Telomeres are simple repeat elements located at each chromosome end of eukaryotic cells. The main function of telomeres is to cap the chromosome end and protect it from enzymatic attack. Telomerase that facilitates the synthesis of telomere has been detected in not only cancer but also precancerous lesion. In this study, we compared the telomerase expression between low grade and high grade colorectal tubular adenoma. Methods: Among thissues from forty eight patients with colorectal tubular adenoma (23 low grade and 25 high grade colorectal dysplasia), telomerase expressions were evaluated by immunohistochemical staining. Results: We classified 48 patients into two groups by the extent of nuclei staining pattern. High telomerase expression was a group which showed staining nucleus pattern above 50% in tubular adenoma. Low telomerase expression was a group which showed staining pattern nucleus below 50%. Twelve in 25 high grade colorectal dysplasia showed high telomerase expression (48%). Only one in 23 low grade colorectal dysplasia showed high telomerase expression (4%). Telomerase expression was much higher in the tissues from the patients with high grade than in those with low grade colorectal dysplasia (p<0.05). Conclusions: Activation of telomerase may be related to the malignant potential in colorectal epithelial cells. Further studies are needed to define the role of telomerase in colorectal tumorigenesis. (Korean J Gastroenterol 2007;50:164-169)

느릅나무 추출물의 항산화 효과 및 L-glutamate 유래 PC12 세포독성 보호효과

최원희(Won Hee Choi),오영상(Young Sang Oh),김성란(Sung Ran Kim),안지윤(Ji Yoon Ahn),하태열(Tae Youl Ha) 한국식품과학회 2005 한국식품과학회지 Vol.37 No.3

기관골 신생증 1예

오영상,이은우,정종혁,문승원,김수현,김동한,양혁승,박정환,박경옥,강성수,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Tracheobronchopathia osteoplastica is a rare disorder of unknown cause characterized by the projection of numerous bony or cartilaginous submucosal nodules into the tracheobronchial lumen with sparing of the posterior membranous portion of tracheobronchial tree. In the past, most of the cases were diagnosed as secondary finding at autopsy. Nowaday, the key component of the evaluation is flexible fiberoptic laryngoscopy and bronchoscopy, which typically demonstrates irregular spicules of subrnucosal bone and cartilage projecting into the tracheobronchial lumen and causing various degrees of airway obstruction. A 45 years old man was visited Sunchon St. Carollo hospital due to discomfort on throat. We saw several nodules just below vocal cord via laryngoscope. The computed tomography revealed multiple small calcified nqdules on trachea and both main bronchi. Pathologic finding of bronchoscopic biopsy showed abnormal proliferation of bony and cartilagious nodules in the tracheal submucosa. These fingdings were consistent with tracheobronchopathia osteoplastica. We report here on a case of tracheobronchopathia osteoplastica with review of literature.

Rifampicin에 의한 것으로 추정되는 위막성 대장염1예

김수현,이은우,정종혁,문승현,김동한,양혁승,오영상,김호동,김도현,박혁,박정환,박경옥,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Pseudomembranous colitis, caused by altering the normal colonic flora and allowing the multiplication of Clostridium difficile, is an deleterious adverse effect of antibiotics. But it is rarely reported by rifampicin. Rifampicin is one of the first line drug in the treatment of tuberculosis and many patients are exposed to its potential adverse effects. We experienced a patient that had abdominal discomfort and hematochezia due to pseudomembranous colitis after receiving antituberculous medication, and which was probably caused by rifampicin. A 82 years old man was admitted with abdominal discomfort and hematochezia for one week. On the past history he had been diagnosed as endobronchial tuberculosis about 4 months ago. Colonoscopy revealed multiple discrete whitish mucosal lesion on rectosigmoid colon, and histologic findings were consistent with pseudomembranous colitis. The antituberculous agents were discontinued and vancomycin was administered. The patient's symptoms were resolved within several days. There was no recurrence after reinstitution of the antituberculous agents excluding rifampicin. We report here on a case of pseudomembranous colitis probably due to rifampicin.