9-12세 정상 아동에서 종골 골밀도와 체성분의 연관성

신은경,김기숙,김희영,이인숙,정효지,조성일,Shin, Eun-Kyung,Kim, Ki-Suk,Kim, Hee-Young,Lee, In-Sook,Joung, Hyo-Jee,Cho, Sung-Il 대한예방의학회 2004 예방의학회지 Vol.37 No.1

Objectives : This cross-sectional study aimed to quantify the relationship between the bone mineral density at the os calcis and the body mass composition in healthy children. Methods : The areal bone mineral density was measured at the os calcis with peripheral dual energy X-ray absorptiometry. The fat free mass, fat mass and percentage fat mass were measured using bioelectric impedance, in 237 Korean children, aged 9 to 12 years. The sexual maturity was determined by self assessment, using standardized series of the 5 Tanner stage drawings, accompanied by explanatory text. Results : From multiple linear regression models, adjusted for age, sexual maturity and height, the fat free mass was found to be the best predictor of the calcaneal bone mineral density in both sexes. About 15 and 20% variabilities were found in the calcaneal bone mineral densities of the boys and girls, respectively, which can be explained by the fat free mass. After weight adjustment, the percentage fat mass was negatively associated with the calcaneal bone mineral density in both sexes. Conclusions : The findings of this study suggest that the fat free mass, among the body compositions, is the major determinant of bone mineral density at the os calcis in Korean children aged 9 to 12 years. Obesity, defined as the percentage fat mass, is assumed to have a negative effect on the calcaneal bone density in children of the same weight.

초경전 여아에서 운동의 기계적 스트레인과 칼슘섭취량에 따른 발꿈치뼈 골밀도의 분포

신은경,김기숙,김희영,이인숙,정효지,조성일,Shin, Eun-Kyung,Kim, Ki-Suk,Kim, Hee-Young,Lee, In-Sook,Joung, Hyo-Jee,Cho, Sung-Il 대한예방의학회 2005 예방의학회지 Vol.38 No.3

Objectives : The effects of exercise on bone density have been found to be inconsistent in previous studies. We conducted a cross-sectional study in premenarcheal girls to test two hypotheses to explain these inconsistencies. Firstly,'the intensity of mechanical strain, in terms of the ground reaction force(GRF), has more important effects on the bone mass at a weight-bearing site', and secondly, 'calcium intake modifies the bone response to exercise'. Methods : The areal bone mineral density was measured at the Os calcis, using peripheral dual energy X-ray absorptiometry, in 91 premenarcheal girls aged between 9 and 12 years. The intensity of mechanical strain of exercise was assessed by a self-report questionnaire and scored by the GRF as multiples of body weight, irrespective of the frequency and duration of exercise. The energy and calcium intake were calculated from the 24-hour dietary recall. An analysis of covariance(ANCOVA) was used to determine the interaction and main effects of exercise and calcium on the bone density, after adjusting for age, weight, height and energy intake. Results : The difference in the bone density between moderate and low impact exercise was more pronounced in the high than low calcium intake group. The bone density for moderate impact exercise and high calcium intake was significantly higher than that for low impact exercise (p=0.046) and low calcium intake, after adjusting for age, weight, height and energy intake. Conclusions : Our study suggests that the bone density at a weight-bearing site is positively related to the intensity of mechanical loading exercise, and the calcium intake may modify the bone response to exercise at the loaded site in premenarcheal girls.

마우스에서 Capillary hepatica 감염에 의한 간섬유증의 병리학적 연구

신은경,한정희,Shin, Eun-kyung,Han, Jeong-hee 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1

This study was performed to investigate the pathogenesis of hepatic granuloma and hepatic fibrosis induced in mice infected with Capillaria(C) hepatica and treated cyclophosphamide. The results were as grossly well-defined yellowish white spots and small nodules at the surface of the liver were scattered. Histopathologically, there were numerous granulomas composed of eggs and fragments of C hepatica surrounded by heavy infiltration of inflammatory cells. Severe fibrosis was observed around granulomas. Pathological lesions of group infected with C. hepatica and then injected with cyclophosphamide were most severe than those of other groups. Therefore this study suggested that hepatic fibrosis induced by C hepatica in mice would be useful for animal model of hepatic fibrosis in human.

Escherichia coli 리보핵산 내부분해효소 RNase E의 돌연변이체 선별 및 특성분석

신은경,고하영,김영민,주세진,이강석,Shin, Eun-Kyoung,Go, Ha-Young,Kim, Young-Min,Ju, Se-Jin,Lee, Kang-Seok 한국미생물학회 2007 미생물학회지 Vol.43 No.4

대장균의 필수적인 리보핵산 내부분해효소인 RNase E는세포내에서 여러 RNA의 분해와 가공과정에서 중요한 역할을 하며, 이 단백질의 효소활성부위를 포함하는 N-말단부위의 498 아미노산(N-Rne)만의 발현으로도 세포의 생장을 가능하게 한다. 이러한 RNase E의 특성을 활용하여 다양한 표현형을 가지는 N-Rne 돌연변이체들을 분리, 동정할 수 있는 효율적인 유전학적 시스템을 개발하였다. 이 시스템을 이용하여 얻어진 효소활성부위 돌연변이체들을 표현형으로 분류하여 분석한 결과, S1 도메인의 6번째 아미노산의 치환(I6T)을 가진 변이체는 야생형 N-Rne의 기능을 대체하지 못하였고, Small 도메인의 488번째 아미노산의 치환(R488C)을 가진 변이체는 야생형 N-Rne의 발현양보다 현저히 작게 발현시켜도 세포의 생장을 정상적으로 가능하게 하였다. 또한 DNase I 도메 인의 305번째 아미노산의 치환(N305D)을 가진 변이체는 야생형 N-Rne의 발현양보다 과발현시켰을 때만 세포의 생장을 가능하게 하였다. 각각의 아미노산 치환을 포함하는 N-Rne를 한정적으로 과발현시켰을 때의 ColEl-타입 플라스미드의 복제 수에 대한 영향을 측정한 결과, 돌연변이체 N-Rne의 세포생장에 대한 영향은 이 변이체들의 세포 내 효소활성 정도에 기인하는 것으로 밝혀졌다. 이러한 실험결과는 이 연구에서 개발한 유전학적 시스템을 이용하여 다양한 표현형을 가진 RNase E 변이체를 선별할 수 있으며, 이 변이체들의 특성을 분석함으로써 RNase E가 RNA의 안정성을 조절하는데 있어서 각각의 세부 도메인의 역할을 규명할 수 있으리라는 것을 시사한다. RNase E is an essential Escherichia coli endoribonuclease that plays a major role in the decay and processing of a large fraction of RNAs in the cell and expression of N-terminal domain consisted of 1-498 amino acids (N-Rne) is sufficient to support normal cellular growth. By utilizing these properties of RNase E, we developed a genetic system to screen for amino acid substitutions in the catalytic domain of the protein (N-Rne) that lead to various phenotypes. Using this system, we identified three kinds of mutants. A mutant N-Rne containing amino acid substitution in the S1 domain (I6T) of the protein was not able to support survival of E. coli cells, and another mutant N-Rne with amino acid substitution at the position 488 (R488C) in the small domain enabled N-Rne to have an elevated ribonucleolytic activity, while amino acid substitution in the DNase I domain (N305D) only enabled N-Rne to support survival of E. roli cells when the mutant N-Rne was over-expressed. Analysis of copy number of ColEl-type plasmid revealed that effects of amino acid substitution on the ability of N-Rne to support cellular growth stemmed from their differential effects on the ribonucleolytic activity of N-Rne in the cell. These results imply that the genetic system developed in this study can be used to isolate mutant RNase E with various phenotypes, which would help to unveil a functional role of each subdomain of the protein in the regulation of RNA stability in E. coli.

KS 시설관리 표준규격을 바탕으로 한 대학시설 FM 서비스 발전방향

신은경,김유진,김준하,Shin, Eun-Young,Kim, Yu-Jin,Kim, Jun-Ha 한국교육시설학회 2015 敎育施設 Vol.22 No.1

Over the years, great attention has been shown to the function of the campus facilities that should be well-equipped, operated and maintained to better support university's two main goals: providing education and supporting research. Properly managing campus facilities is the initial step to be prepared to meet the rapidly changing global educational standard. However, so far, no definitive answer has been given to the question of what service level/scope is appropriate for the effective campus facility management (FM) in Korea. Therefore, at the outset, it is imperative to establish the framework for the standardized campus FM services. The main focus of this research is to provide comprehensive understanding on the campus FM by establishing the framework in the form of FM performance indicator (PI). As the first step, FM standards set by Korean Agency for Technology and Standards (KATS) has been thoroughly reviewed and analyzed to establish the framework for FM standards applicable to campus facility management. Secondly, extensive literature review on the development of performance indicator (PI) for the educational facilities has been conducted to classify PI types and complement FM_KS. Finally, specific guideline and PIs developed by APPA which is globally well-known association in the field of FM for higher education facilities has been added to the combined PI which is the mixed form of FM_KS and PIs from the previous literature. This research provides campus facility managers and FM outsourcing service providers with framework in the form of PI to support future directions of effective campus FM services.

대식세포의 식세포활동과 화학주성에 대한 인삼분획물의 영향

신은경,김세창,Shin, Eun-Kyoung,Kim, Sei-Chang 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

쥐의 복강과 폐포 대식세포를 분리하고 배양하여 효모에 대한 식세포활동 및 화학주성을 관찰하였다. 식세포활동이 일어나는 양상을 wright-Giemsa로 염색하여 사진으로 확인하였다. 복강 대식세포의 경우 diol saponin 처리군에 의해 약 48%까지 식세포활동이 증가되었고 total saponin 처리군에 의해 약 35%까지 식세포활동이 감소되었다. 폐포 대식세포의 경우 모든 인삼분획물 처리군에 의해 최고 50%까지 섭식이 증가되었다. 화학주성은 복강 대식세포의 경우 인삼분획물 처리군 모두 약 17%까지, 폐포 대식세포에서는 diol saponin만 약 16%까지 이동되었다. actin의 증감을 SDS-PAGE로 확인해 보았으나 변화가 없었다. The phagocytosis and chemotaxis of murine macrophages after treated with saponin fractions are investigated. Phagocytic appearance against yeast was photographed by dying with Wright-Giemsa. Phagocytic activity of peritoneal macrophage was invreased in diol saponin treatment by 48% and was decreased in total saponin treatment by 35%. The ingestion of alveolar macrophage was increased by 50% maximally. Peritoneal chemotactic activity was shown in 17% increases and only diol saponin had effect in alveolar macrophage by 16%. According to SDS-PAGE method the contents of actin did not show any alterations.

대식세포의 화학주성과 세포내 칼슘과 Actin의 증가에 미치는 인삼사포닌 성분의 영향

신은경,김세창,Shin, Eun-Kyoung,Kim, Sei-Chang 배재대학교 자연과학연구소 1998 自然科學論文集 Vol.10 No.1

본 실험은 대식세포 화학주성과 세포내 칼슘과 F-actin 증가에 대한 인삼사포닌 분획의 영향을 알아보기 위하여 수행되었다. 여러 가지 인삼사포닌 분획을 처리한 복강 대식세포는 대조군에 비해 화학주성이 28.4-71% 증가하였다. 세포내에 유리된 칼슘의 양은 65%까지 증가하였으며, NBD- phallacidin을 처리한 세포에서 F-actin의 양은 10% 증가하였다. 칼슘이나 PMA로 활성화시키고 사포닌 분획을 처리하였을 때, F-actin의 양은 현저하게 증가하였으며 이러한 현상은 2분까지 지속되었다. 이러한 결과로 보아 인삼사포닌 분획이 chemoattractant로 작용할 수 있을 것으로 생각된다. In the present study, We have tested the potential effects of ginseng saponin fractions on macrophage chemotaxis and intracellular calcium and F-actin mobilization. Peritoneal macrophages treated with various ginseng saponin fractions showed 28.4% to 71% of increasement of chemotaxis as compared with untreated cells. The activity of intracelluar calcium mobilization was increased up to 65% by treatment with saponins, and F-actin content also increased 10% in the cells loaded with NBD-phallacidin. When the cells were activated with calcium of PMA and treated with saponin fractions, the intracelluar F-actin content increased significantly and prolonged for 2 minutes. These results suggest that ginseng saponin fractions might be a chemoattractants.

딥러닝 기반의 최대응력과 위치 예측 기법: 로드 휠 충격 테스트 예시

진아현(Ah-hyeon Jin),이성희(Sunghee Lee),유소영(Soyoung Yoo),신승연(Seungyeon Shin),김창곤(ChangGon Kim),허성필(Sungpil Heo),강남우(Namwoo Kang) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.8

The impact test of road wheels is one of the important tests to ensure the safety of the wheels. Automakers use CAE simulation to analyze the location and magnitude of maximum stress, reducing prototype testing costs and time. However, the time required for modeling and analysis is still large, so it is difficult to quickly evaluate a large amount of conceptual design. In addition, it is difficult for general engineering designers to utilize because it requires high expertise in CAE. This study develops an AI-based wheel performance evaluation process that uses deep learning with CAE data to learn the location and magnitude of maximum stress. This deep learning model can predict the strength performance of road wheels in real time, allowing rapid evaluation at the conceptual design stage without domain knowledge.

SOM과 LSTM을 활용한 지역기반의 부동산 가격 예측

신은경 ( Shin Eun Kyung ),김은미 ( Kim Eun Mi ),홍태호 ( Hong Tae Ho ) 한국정보시스템학회 2021 情報시스템硏究 Vol.30 No.2

Purpose The study aims to predict real estate prices by utilizing regional characteristics. Since real estate has the characteristic of immobility, the characteristics of a region have a great influence on the price of real estate. In addition, real estate prices are closely related to economic development and are a major concern for policy makers and investors. Accurate house price forecasting is necessary to prepare for the impact of house price fluctuations. To improve the performance of our predictive models, we applied LSTM, a widely used deep learning technique for predicting time series data. Design/methodology/approach This study used time series data on real estate prices provided by the Ministry of Land, Infrastructure and Transport. For time series data preprocessing, HP filters were applied to decompose trends and SOM was used to cluster regions with similar price directions. To build a real estate price prediction model, SVR and LSTM were applied, and the prices of regions classified into similar clusters by SOM were used as input variables. Findings The clustering results showed that the region of the same cluster was geographically close, and it was possible to confirm the characteristics of being classified as the same cluster even if there was a price level and a similar industry group. As a result of predicting real estate prices in 1, 2, and 3 months, LSTM showed better predictive performance than SVR, and LSTM showed better predictive performance in long-term forecasting 3 months later than in 1-month short-term forecasting.

구연발표 : 나트륨 측정 방법에 따른 대구시민의 일일 나트륨 섭취량 비교

신은경 ( Eun Kyung Shin ),이연경 ( Yeon Kyung Lee ) 대한지역사회영양학회 2008 대한지역사회영양학회 춘-추계학술대회지 Vol.2008 No.2