한국 특산 Thalictrum속 식물의 성분 연구

허봉희 서울保健大學 1985 論文集 Vol.5 No.1

우리나라 特産植物인 "산꿩의 다리"의 지하경을 methanol로 抽出하고, 이 抽出物을 減庄濃縮하여 Citric acid에 녹이고 이것을 NH_(4)OH로 pH9-10으로 한 後 ether Chloroform 可溶部와 水溶層으로 나누고 水溶尸 을 取하여 Reineckate鹽으로 만든 後 四級 alkaloid鹽을 沈澱시켜 (約I04g) 50% acetone水溶液에 녹이고 濾過해서 不溶性物質을 除去했다. 濾液을 陰ion 交換樹脂에 통과시켜 鹽化物을 얻었다. 鹽化物을 증발 乾固해서 賠褐色物質을 約 27g을 얻었다. 이것을 뜨거운 methanol에 녹여 不溶性物質을 除去하고 減庄濃縮한 殘溜 約 24.3g을 얻어 이 物質을 여러 spectrum 시험을 通해 그 化學구조를 究明한 結果 四級 alkaloid인 magnoflorine Chloride임을 同定하였다. Three kinds of alkaloids were isolated from the roots of Thalictrum tuberiferum Maximowicz which is one of the plants indigenous to our country. These alkaloids belong to aphorpline alkaloid. Among them a brownish crystalline substance showed mp 250-252°C, C_(20)H_(24)O_(4)^(+) Cl^(-) of molecular formula was identified to he magnoflorine Chloride through the UV, IR, NMR, and mass spectrum. Further study on the bioactivity of previous alkaloid must be followed. And continual identification of another alkaloids must be followed too.

殺菌製 Benomyl이 콩 發芽에 미치는 영향

허봉희 서울保健大學 1986 論文集 Vol.6 No.1

입자 크기에 따른 ITO 가스 센서의 가스 감지 특성

신동원,김봉철,전희권,이상태,이덕동,허증수 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

당뇨병 환자에서 혈장 Thrombin-Antithrombin Ⅲ 및 Plasmin-α_2-Plasmin Inhibitor 복합체의 임상적 의의

김경욱,김은숙,정상수,윤수지,박우일,이준희,남수연,안철우,문병수,김경래,차봉수,송영득,임승길,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:당뇨병 환자에서 혈액응고 및 섬유소용해 체계의 이상경향이 있어 그 결과로 여러 혈관합병증의 발생위험이 높다는 사실은 널리 알려져 있다. 그 기전은 아직 확실히 밝혀지지 않았으나, 고혈당으로 인한 혈장 단백질들의 비효소성 당화작용이나 산화성 스트레스로 인한 유리 라티칼 작용으로 응고항진이나 섬유소용해 활성의 저하를 유발하는 것으로 생각되고 있다. 최근 응고 및 용해인자와 그 억제자의 복합체들의 증가가 이 상태를 비교적 예민하게 반영한다고 알려져 있다. 방법:본 연구에서는 당뇨병 환자 101명과 정상 대조군 20명에서 혈장내 thrombin­antithrombin complex(TAT)와 plasmin­α₂­plasmin inhibitor complex(PIC)를 측정하여 비교하고, 당뇨병 환자에서 미세혈관 합병증과 대혈관합병증의 유무에 따른 차이와, 이미 혈관 질환의 위험인자로 알려져 있는 인자들간의 상관성을 알아보고자 하였다. 결과:1. 환자의 분포를 살펴보면 혈관합병증이 있는 군은 85명, 혈관합병증이 없는 군은 16명이었고, 평균연령은 각각 57.9±14.1세, 49.9±16.6세로 혈관 합병증이 있는 군에서 더 나이가 많았고, 체질량지수는 23.2±3.4㎏/㎡, 24.1±3.4㎏/㎡로 두 군간 유의한 차이는 없었다. 또 두 군간의 혈압 및 HbA1c, 공복혈당 및 인슐린과 C­peptide, 총 콜레스테롤, 중성지방, HDL­콜레스테롤, Lp⒜는 유의한 차이가 없었고, 미세혈관합병증이 있는 군에서 당뇨병의 유병기간이 길었다. 2. TAT 및 PIC의 농도는 정상 대조군에서는 2.8±1.2 ng/mL, 240.4±69.7 ng/mL이었고, 당뇨병 환자군에서는 9.5±22.6 ng/mL, 472.2±258.7 ng/mL이었다. TAT와 PIC 모두 당뇨병 환자군에서 정상 대조군에 비해 유의하게 증가되어 있었고(p<0.001), TAT/PIC ratio는 두 군간 차이가 없었다. 3. 당뇨병 환자의 혈관합병증에 따른 TAT 및 PIC, fibrinogen 농도는 합병증이 없는 군은 각각 4.1±2.4ng/mL, 362.2±272.0ng/mL, 322.7±102.4mg/mL으로 PIC와 fibrinogen의 증가를 보였으나, 연령을 보정한 후에는 통계학적 유의성은 없었다. 또 대혈관 합병증군에서는 각각 6.0±4.9 ng/mL, 507.4±321.6 ng/mL, 427.1±194.7 mg/dL이었으며 미세·대혈관 합병증군에서는 10.4±6.4 ng/mL, 484.8±269.7 ng/mL, 388.4±132.4 mg/dL으로 TAT의 증가를 보였으나 역시 연령을 보정한 후에는 통계학적 유의성은 없었다. 4. 미세혈관합병증군에서 HbA1c(>8%)가 높은 군의 PIC 농도가 유의하게 높았고(p=0.049), 대혈관합병증군에서 HbA1c(>8%)가 높은 군의 총 콜레스테롤 농도가 유의하게 높았다(p=0.042). 5. 총 당뇨병 환자군에서 PIC는 fibrinogen과 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며(r=0.47, 0.31,-0.25), 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다(r=0.67). 결론:이상의 결과에서 혈장 TAT 및 PIC 농도는 당뇨병 환자에서 정상 대조군에 비해 의미있게 증가되어 있었고, 당뇨병 환자군에서는 연령의 증가와 유병기간이 혈액응고항진 및 용해의 장애에 큰 역할을 함을 알 수 있었으며, 총 당뇨병 환자군에 PIC와 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다. 따라서 당뇨병 환자에서 혈액응고 및 용해의 장애가 동반되어 있다고 볼 수 있으며, 혈장 TAT 및 PIC는 혈관합병증으로의 진행을 예측하는 지표로서 유용하리라 생각된다. 또 혈당조절정도와 상관성이 있으므로 혈당조절후에 추적검사를 시행하여 합병증의 예방이 가능한지 추후 연구가 필요하리라 생각된다. Background : Abnormality of coagulation and fibrinolystic system is known as a predisposing factor of vascular complication in diabetes. Although the pathogenesis is not well known, non-enzymatic glycation reaction and the increase in production of free radicals due to an increased oxidative stress may be linked to the hypercoagulibility and hypofibrinolytic activity. As indices of abnormality in coagulation and firinolysis in peripheral blood, plasma thrombin-antithrombin Ⅲ complex (TAT) and plasmin-α_2-plasmin inhibitor complex (PIC) were measured. The purpose of this study was to clarify whether hypercoagulability exists in diabetic patients with or without vascular complication. Methods : In our study, we measured plasma thrombin-antithrombin Ⅲ compelx (TAT) and plasmin-α_2-plasmin inhibit or complex (PIC) in 101 diabetic subjects and 20 controls. Comparing TAT and PIC levels in diabetic microvascular complication group, diabetic macrovascular complication group and controls, we examined correlation between risk factors associated with diabetic vascular complication. Results : 1. The group with diabetic vascular complication was older than group without complication. There was no significant difference in BMI, blood pressure, HbA_ic, blood sugar level, insulin, C-peptide, serum creatinine, total cholesterol, triglyceride, HDL-cholesterol, Lp (a) between two groups. The group with diabetic microvascular complication had longer duration of diabetes. 2. Concentration of TAT and PIC were 2.8±1.2 ng/ mL, 240.4±69.7 ng/ mL in controls and 9.5±22.6 ng/ mL, 472.2±258.7 ng/ mL in diabetic patients, respectively. TAT and PIC were significantly higher in diabetic patients than in control (p<0.001). But TAT/PIC ratio was no significant difference between two groups. 3. In diabetic patients, concentration of TAT and PIC and fibrinogen were respectively 4.1±2.4 ng/ mL, 362.2±272.0 ng/ mL, 322.7±102.4 mg/ dL in group without vascular complication and 5.3±4.1 ng/ mL, 529.5±258.7 ng/ mL, 374.9±106.2 mg/ dL in group with microvascular complication, which group had increase in PIC and Fibrinogen but no significance after correction of age. Concentration of TAT and PIC and Fibrinogen were 60.±4.9 ng/ mL, 507.4±321.6 ng/ mL, 427.1±194.7 mg/ dL in macrovascular complication, and 10.4±6.7 mg/ mL, 484.8±269.7 ng/ mL, 388.4±132.4 mg/ dL in combined vascular complication which group showed increase of TAT but also had no significant increase after correction of age. 4. In diabetic microvascular complication patients, group of high HbA_1c (>8%) (p=0.049) had significant high PIC concentration. In diabetic macrovascular complication patients, group of high HbA_1c (>8%) (p=0.042) had significant high total cholesterol concentration. 5. In all diabetic patients, PIC was positively correlated with fibrinogen and HbA_1c and negatively correlated BMI (r=0.47, 0.31, -0.25). Only in daibetic patients without angiopathy, TAT was positively correlated with HbA_1c (r=0.67). Conclusion : In this study, plasma TAT and PIC concentration significantly increased in diabetic patients compared with controls, and PIC was increased in group with microvascular complication, TAT were increased in group with combined micro macrovascular complication. However, there was no significance relationship existed when correctinf for age. PIC was correlated with HbA_1c. TAT was correlated with HbA_1c only in the group without angiopathy. Abnormality of coagulation and fibrinolysis were combined in diabetes, plasma TAT and PIC can be used as an index of vascular complication. Also we found the correlation with the degree of the blood glucose control. Therefore we need follow up study for the possibility of prevention of vascular complication after controlling the blood glucose to age-matched patients (J Kor Diaabetes Asso 25:354~363, 2001).

Lingans from Korean Red Ginseng

Huh, Bong-Hee,Lee, Ihn-Ran,Han, Byung-Hoon The Pharmaceutical Society of Korea 1990 Archives of Pharmacal Research Vol.13 No.3

Two lingans were isolated from hexane-soluble fraction of Korean red ginseng. Their chemical structures were elucidated as gomisin N and gomisin A by spectrometric analysis.

Equivalent circuit model of a rectangular RF driven hydrogen ion source for impedance matching network design

Huh, Sung-Ryul,Park, Min,Jung, Bong-Ki,Chang, Doo-Hee,Kim, Tae-Seong,Kim, Sun-Ho,Jeong, Seung Ho Elsevier 2018 Fusion engineering and design Vol.136 No.2

<P><B>Abstract</B></P> <P>KAERI is presently developing a rectangular RF driven deuterium ion source for both neutron source and fusion applications. For a model-based design of a reliable impedance matching network for the ion source, we extend an equivalent circuit model of the ion source system by allowing effects of an isolation transformer and a Faraday shield on the system to be reflected in our previous model (Huh et al., 2017 <I>Fusion Eng. Des.</I> <B>121</B> 337). In this paper we use the model to investigate the trends in external electrical characteristics of the ion source system (e.g., voltage and current across and through an RF antenna coil and the impedance matching network circuit components) with RF power of up to 50 kW. The model results provide criteria for the impedance matching network design and data for an assessment of the ion source performance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An equivalent circuit model of a rectangular radio frequency driven hydrogen ion source is developed. </LI> <LI> This model predicts electrical characteristics of the ion source equipped with an isolation transformer and a Faraday shield. </LI> <LI> The model is used to design of an impedance matching network for the KAERI rectangular radio frequency driven ion source. </LI> </UL> </P>

Anit-HBc 단독 양성자의 anti-HBs 양전에 영향을 미치는 요인

허봉렬,조비룡,서상연,고희정,이윤,정범,명승권 대한가정의학회 1999 Korean Journal of Family Medicine Vol.20 No.6

Preliminary design of an impedance matching circuit for a high power rectangular RF driven ion source

Huh, Sung-Ryul,Park, Min,Jung, Bong-Ki,Chang, Doo-Hee,Kim, Tae-Seong,Kim, Sun-Ho,Jeong, Seung Ho North-Holland 2017 Fusion engineering and design Vol.121 No.-

<P><B>Abstract</B></P> <P>Within the framework for the development of a radio frequency driven positive ion source for fusion applications, KAERI is currently constructing a new high power large area radio frequency ion source. An impedance matching circuit maximizing the power transfer to a plasma in the ion source, while taking into account the technical feasibility, is preliminarily designed at a conceptual level. In order to estimate the plasma load impedance and design parameters, an integrated model composed of the hydrogen plasma global model, the two-dimensional electromagnetic model, the relative plasma dielectric constant calculation module, and the impedance matching circuit module is developed for the ion source with a rectangular tube geometry. By employing this design model, the design parameters dependent on RF input powers of up to 50kW are investigated and the preliminary design is assessed.</P>

Selection of Early Flowering Plants by Transformation of the DgLsL Anti-sense Partial Gene in Chrysanthemum (Dendranthema grandiflorum Kitamura) Zinba

Bong Hee Han,Su Young Lee,Eun Joo Huh 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

질병이 가족에게 미치는 영향에 관한 연구 : 백혈병 환아의 부모에 대한 GHO 및 Family APGAR결과를 중심으로 The Application of General Health Questionnaire & Family APGAR to Parents of Childhood Leukemia Patients

허봉렬,김창엽,서홍관,황인홍,박훈기,박희순,김용은 대한가정의학회 1990 Korean Journal of Family Medicine Vol.11 No.4