Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

UV/Persulfate 산화 및 전기분해 방법을 이용한 TMAH 용액 및 부산물 처리 연구

최효연 ( Hyo-yeon Choi ),이용호 ( Yong-ho Lee ),이효주 ( Hyo-joo Lee ),이수민 ( Su-min Lee ),박대원 ( Dae-won Pak ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2019 No.-

현재 TFT-LCD를 제조하는 과정에서 다양한 종류의 화학약품을 사용하며 이로 인해 다양한 종류의 폐수가 다량 발생되고 있다. 이 중 TMAH(Tetra methyl ammonium hydroxide)는 유입되는 질소의 주요 성분으로 TFT-LCD 제조 공정에 있어서 핵심 공정 중의 하나인 Photo 공정 중 포토 레지스트(photo resist)의 현상액으로 사용되는 강알칼리의 유기 아민계 용액으로, 무색, 투명의 강염기성 물질이고, 수용성, 가연성의 성질을 나타낸다. 또한 그 농도가 증가하면서 강한 독성을 배출하여 생물학적 분해가 어렵고, 분자 구조 속에 질소를 포함하고 있어, 분해 시 암모니아성 질소를 생성하는 특성을 가지고 있다. 이에 따라 자연 수중 생태계에 영향 등의 문제가 발생되기 때문에 TMAH 폐수의 효율적인 처리방법 도출이 필요한 사항이다. 현재 TMAH를 함유한 폐수는 호기성 생분해, 혐기성 생분해, 촉매 산화, 광촉매 분해(UV 조사) 또는 이온 교환 기술 등으로 처리되고 있습니다. 그 중 UV 조사는 과황산염 활성화를 위한 에너지의 한 형태로 적용되며 난분해성 분해에 많이 적용되고 있다. 과황산음이온(S₂O₈ ^2-)은 산화환원전위가 2.01 V인 강한 산화제이며 토양과 지하수에 존재하는 유기오염물질을 분해하는데 효과적이라고 한다. 과황산염 반응 메커니즘은 다양한 과황산염 활성화를 통한 강한 황산염 라디칼(SO₄^-·) 및 하이드록실 라디칼(HO^·)의 생성에 기반을 두고 있으며 1 mole의 황산염은 자외선 조사를 통해 2 mole의 황산염 라디칼을 생성하도록 활성화 될 수 있다고 보고되어 다양한 난분해성 유기 오염 물질을 분해에 적용되고 있다. 전기화학적 산화 처리공정은 고분자량을 가지는 화합물을 파괴하며 난분해성 유기물질의 독성을 감소시킬 수 있어 효과적인 것으로 제시되고 있으며, 고도처리공정들 가운데 오염물질 생분해성과 관계없이 높은 제거율과 처리시간이 짧은 운전특성을 나타내고 있어 유기화합물 또는 질소화합물의 제거에 있어서 응용가능성이 높은 것으로 평가되고 있다. 따라서, 본 연구에서는 TMAH 모형폐수를 제조하여 먼저 과황산염(S₂O₈ ^2-)을 이용한 UV 산화방법으로 TMAH를 분해하고 그에 따른 부산물을 전기분해 방법으로 처리하고자 하였다.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

기존 부적격 건축물의 특례제도에 관한 연구 : 한일간의 비교법적 고찰을 통한 우리 특례 규정의 정비방안을 중심으로

조연팔,최철호 梨花女子大學校 法學硏究所 2016 法學論集 Vol.20 No.4

사회 정세의 변화에 대응하기 위하여 ｢건축기준법｣상의 규제를 보다 엄하게 정하는 경우, 신법령의 행정목적과 건축주의 기득권의 조정을 고려해, 입법 정책적으로 ‘현존 건축물’과 ‘공사중’인 건축물에는 구법령을 적용하는 것으로 하였다. 이것이 일본의 기존 부적격 건축물 제도이다. 이 중 ‘공사중인 건축물’의 해석에는 ‘착공’ 이후가 ‘공사중’인 건축물에 해당한다는 착공설과 인공 구조물인 건축물의 존재를 가지고서 ‘공사중’인 건축물에 해당한다는 건축물설이 있다. 일본의 판례는 건축물설에 따른 경우도 있으나 최고재판소는 착공설에 따라 판단하였다. 우리 ｢건축법｣ 제6조나 ｢국토계획법｣ 제82조 등에서도 기존 건축물의 특례제도를 인정하고 있다. 먼저 이중에서 신축에 대하여 보면 우리 법령은 신축에 대하여서는 함구하고 있으나 처분의 위법성 판단 시에 대한 통설 판례의 입장이 처분시설인데 이 견해에 의하면 우리 ｢건축법｣ 제6조나 ｢국토계획법｣ 제82조에서 신축의 경우에도 이미 허가를 받은 경우에는 법령의 제정이나 개정과 상관없이 계속하여 허가받은 사항대로 건축공사를 할 수 있다는 의미에서 기존 건축물의 특례를 인정하고 있다고 할 수 있다. 따라서 문제가 되는 것은 강화된 규정이 입법 예고된 상태에서 실제 개정된 이후의 불이익을 예상하여 예고기간이 끝나기 전에 건축허가를 신청하는 끼어들기 건축허가신청을 막을 길이 없다는 점이다. 따라서 ｢국토계획법｣ 제82와 ｢건축법｣ 제6조에서는 신축에 대하여 어느 범위까지 기존 건축물의 특례를 인정할 것인가를 명확히 할 필요가 있다. 입법론적으로는 ｢국토계획법｣ 제31조 제2항과 마찬가지로 ‘이미 사업이나 공사에 착수한 때’로 한정하여 끼어들기 건축허가신청을 방지할 필요가 있다고 여겨진다. 이 때 공사의 착수시점을 언제로 보아야 하는가의 문제가 있지만 이 경우에는 일본의 사례에서 시사를 받을 필요가 있다고 생각된다. 다음으로 증･개축에 대하여 보면 증･개축의 경우에도 일본처럼 일정한 경우에는 그것을 완화하는 규정을 둘 필요가 있다고 생각된다. 그리고 우리 ｢국토계획법｣ 제31조 제2항에서는 “도시･군관리계획 결정 당시 이미 사업이나 공사에 착수한 자는 그 도시･군관리계획 결정에 관계없이 그 사업이나 공사를 계속할 수 있다”고 규정하고 있는데, 문제는 도시･군관리계획 결정만을 그 근거로 하고 있고 법령의 제정이나 개정 당시 사업이나 공사에 착수한 자도 그 사업이나 공사를 계속할 수 있는지에 대하여서는 함구하고 있다. 하지만 법령의 제･개정과 도시계획의 결정 또는 변경과의 사이에 차이를 두어야 하는 합리적인 이유가 없다고 여겨지기 때문에 법령의 제정이나 개정의 경우에도 도시･군관리계획 결정과 마찬가지로 이미 사업이나 공사에 착수한 자는 사업이나 공사를 계속할 수 있다고 하는 것이 타당해 보이지만, 동 규정이 ‘도시･군관리계획 결정의 효력’인 관계로 여기에 법령의 제정이나 개정 시에도 사업이나 공사를 계속할 수 있다는 규정을 두는 것은 법률의 체계상 타당하지 아니하다. 따라서 ｢국토계획법｣ 제31조 제2항을 제82와 함께 묶어서 규정하는 것도 고려해 봄직하다. 다만 ｢국토계획법｣ 제31조 제2항은 효력규정이어서 기존건축물의 특례에 대하여 정하고 있는 제82조와 함께 규정하는 것에는문제가 있다고 생각할 수도 있겠지만 한편으로 ｢국토계획법｣ 제31조 제2항은 기존 건축물의 특례에 관한 규정이라고 볼 여지도 있어 제31조 제2항을 제82조와 함께 묶어서 규정하는 것은 입법 방법론적인 문제라고 생각된다. It may prescribe regulation more strictly to cope with the change of social conditions. It is necessary to consider the administrative purpose of new laws and ordinances and the protection of vested rights then. Therefore, decided to apply old laws and ordinances by the building law for the building (include an existing building) under construction. This is a Japanese existing building exception system. Interpretation under construction includes two opinions. first is a start of construction theory. This is an opinion that start of construction afterward is under construction. There is a building theory next. This is an opinion to be under construction with the existence of the building which is an artificial structure. Part of the precedent had depended on the building theory, but judged the Supreme Court is as start theory of the construction. Korea establishes an existing building example system, too. (For example, building law Article 6, land development program law Article 82). At first it is new construction. The Korean laws and ordinances do not establish a rule for new construction. The common view (precedent) looks at the time of the illegal judgment at the time of disposal. Therefore, it may be said that recognize the exception of the existing building in construction law Article 6 in Korea according to this opinion in the case of new construction. This is because building works are possible regardless of an establishment and the revision of laws and ordinances successively when receive permission. Therefore, it is as follows to become the problem. In other words, expecting a disadvantage after the revision, It means that cannot block a planning permission application during a notice period. Therefore, it is necessary to recognize the exception of the existing building in land development program law Article 82 or building law Article 6 to which range for new construction, or to make it clear. It is thought that it is necessary to prevent permission application to do it like land development program law Article 31, and to interrupt if I say for the legislation idea. But it becomes the problem to decide start of the construction time. In this case, it is thought that it is necessary to receive current events in a Japanese example. Next is a problem for extension and reconstructionnext. It is thought that it is necessary to put a rule to relieve it like Japan in the case of extension and reconstruction when it is constant. And it establish it as follows in land development program law Article 31 Clause 2 of Korea. In other words, “at the time of decision of the city planning, the person who made start of the construction can follow by the construction regardless of city planning decision.” But it is as follows to become the problem. In other words, it prescribes the city planning decision, but does not prescribe it for the revision of the law. However, it is thought that there is not the rational reason that must establish a difference for the establishment of laws and ordinances and the decision of the city planning. Therefore, it is thought that it is proper to do as well as city planning decision in the case of the legislation. Finally it is thought that it is necessary to tie up land development program law Article 31 (Clause 2) with land development program law 82, and to prescribe it.

닭의 능력검정정보 통계분석시스템 개발

최연호,서강석,조상문 한국농업정보과학회 2000 농업정보과학 Vol.1 No.2

This study was carried out to develop RSTASystem (Random Sample Test Analyses System) and internet homepage for providing chick random sample test infermation to rhick breeders and farmers. RSTA-System is the applied statistical package sofhvare which can be used to analyse the random sample test data collected since 1985 by the chick random sample test station in Ansung. Microsoft Visual Basic(V6.0) was used for developing RSTASystem, and the system was composed of 4 main menus and 10 sub-menus. Random sam-ple test information acquisited by RSTASystem could be provided through the internet homepage (agis.nlri.go,kr/www.poultry.or.kr). Major contents are as fnllows : introduction and schedule of random sample tesL summary reports from 1910 to 1999 for tapers and broilers, full reports from 1990 to 1999 for layers and broilers, down-load of RSTASystem fnes and database file. Users could acquire random sample test data and relevant information easily and quitkly through this system, and the information would be applied for selecting chick strains and producing of making more profits.

급박 하지 허혈증에 대한 비수술적 치료의 장기 추적 결과

최재영,손민수,이길연,이상목,고석환,홍성화,윤층,오수명,박호철 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.1

흰쥐 십이지장 점막의 Polyamine 대사, [^(14)C]Spermine 단백 결합능 및 [³H]Thymidine DNA 섭취에 대한 Calcitriol과 Prednisolone의 상호작용

최상현,이영재,정휘정,김형건,신경호,천연숙,전보권 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.1

Calcitriol has been shown to induce the duodenal synthesis of the spermine-binding protein. and hydrocortisone was reported to increase the intestinal polyamine contents. This study examined whether prednisolone might enhance or reduce the calcitriol effects on the polyamine contents, [^(14)C]spermine-protein binding activity, and [^(3)H] thymidine DNA synthesis of the rat duodenum. The mucosal contents of putrescine. spermidine. and spermine were increased by 198.8%, 84.0%, and 78.3%, respectively, within 6 hours after calcitriol. 5 ㎍/kg (CT) and fell down to the control values at 12 hours. And prednisolone. 10 mg/kg (PD) also increased the polyamine contents in similar patterns. They did not counteract each other in the polyamine metabolism. CT increased the [^(14)C]spermine-binding activity of the cytosol protein fraction from the duodenal mucosa by 71.1%. But PD increased the binding activity by 28.0% and did not affect the CT-induced increase. CT increased the [^(3)H]thymidine DNA synthesis of the duodenal mucosa by 42.3%. but PD reduced it slightly. The CT-dependent increase of the synthesis was significantly inhibited by PD. These results suggested that the inhibitory effect of glucocorticoids on the vitamin D dependent increases of the duodenal calcium absorption and mucosal proliferation seems not to be associated with the metabolic changes of polyamines in the duodenal mucosa.

구미산업단지 대기중 휘발성유기화합물(VOCs)의 농도특성

최우건,배상호,박덕신,정연구,김태오 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

This study focuses on the measurement of airborne Volatile Organic Compounds (VOCs) in the Kumi electronic industrial complex during the time periods of August and September, 2002 and January and February, 2003. This study was based on the US-EPA method TO-14 while the VOCs were analyzed with GC/MSD. The toluene level revealed high concentration at all measurement sites. The areal rank of average concentrations of VOCs is as follows : industry l>industry2>urban>middle>residential. Concentrations of VOCs in Kumi electronic industrial ones were generally higher than at Yeochon and Ulsan industrial complexes. Dichloromethane and trichloroethylene, which are used as a cleaner in the process of electronic industries, were observed 4 to 8 times higher than those of other areas. Among the aromatic compounds, toluene showed the highest level, while the concentrations of dichloromethane and trichloroethylene were higher than those of other halogen compounds. In Kumi, toluene, trichloroethylene, and dichloromethane were confirmed as the major compounds of VOCs by this research.

Starter 및 멸치액젓 첨가가 김치양념 및 겉절이 김치의 품질에 미치는 영향

최택권,박소희,유진현,임호수,조재선,황성연 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.2

양념에 Leu. mesenteroides, Lac plantarum의 젖산균과 멸치액젓 첨가가 김치 양념의 대장균군 제어, 속성촉진여부 및 관능적 특성에 미치는 영향을 조사하였다. Leu. mesenteroides과 젓갈을 첨가한 경우 초기 숙성이 촉진되나 후기 산패는 완만하였으며 Lac. plantarum의 첨가시에는 초기 숙성에는 큰 차이가 없고 후기 산패가 현저하게 진행되었다. 대장균군은 산도 0.80% 이상에서 사멸되었는데 leu. mesenteroides의 경우가 숙성이 촉진되어 보다 신속히 사멸시켰다. 총균수와 젖산균수는 초기에 starter 첨가구는 10^6CFU/mL 정도인데 반해서 starter를 첨가하지 않은 않은 처리구에서 총균수는 10^6CFU/mL , 젖산균수가 10^4CFU/mL 이었고 숙성 후에는 첨가균이 높은 수준을 유지하였다. 하지만 대체적으로 스타터의 첨가가 숙성 및 대장균에 크게 영향을 주지 못하는 경향으로 동일하게 나왔다. 관능적 특성에서는 Leu. mesenteroides 첨가구가 Lac. plantarum의 경우보다 우수하였다. 한편, 시판 겉절이 김치는 산도가 0.09~0.18%, ph 5.46~5.89, 총균수 2.2×10^6~3.1×10^7CFU/mL, 젖산균수는 2.1×10^4~6.6×10^5CFU/mL, 대장균수는 1.8×10^4~7.1×10^5CFU/mL, 염분 1.89%~2.51%으로 스타터 첨가 겉절이에 비해 ph와 염도를 제외한 품질 특성에서 전반적으로 낮은 값을 나타냈다. This study was performed in order to investigate the effect of Leuconostoc mesenteroides, Lactobacillus plantarum and salt fermented anchovy extracts on Kimchi sauce. The sauce was fermented more rapidly by addition of Leu. mesenteroids or salt fermented anchovy extracts on the early fermentation stage than the control, but it was fermented slowly on the late fermentation stage. When L. plantarum was added to the Kimchi on the early fermentation stage, the acidity of Kimchi didn't show a significant difference from the control, but acidity was remarkably increased on the late stage. Coliform group was disappeared when acidity of sauce was higher than 0.8% during fermentation. It was controlled by Leu. mesenteroides but not by L. plantarum. Total count and lactic acid bacterial count of the sauces with starter were 6.30×10^6~1.0×10^7 CFU/mL and 1.04~2.04×10^6 CFU/mL, respectively, but those of the control sauce were 10^6 CFU/mL and 10^4 CFU/mL, respectively. Those count of the sauce with starter were higher than those of the control sauce on the later stage of fermentation. Organoleptic quality of the sauce with Leu. mesenteroides was superior to that with L. plantarum.