축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

Ginger Extract Ameliorates Lipid Accumaulation and Endoplasmic Reticulum Stress of Adipose Tissues by SIRT-1-PGC-1α Signaling via AMPK Activation in ob/ob mice

Hwa-Jin Kim,Han-Jung Chae,Geum-Hwa Lee,Soon-Yeon Jeong,Seon-Ah Park,Hwa-Young Lee,The-Hiep Hoang,Young-Je Lim,Ji-Hyun Kim 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

S100A8 and S100A9 promotes invasion and migration through p38 mitogen-activated protein kinase-dependent NF-κB activation in gastric cancer cells

Kwon, Chae Hwa,Moon, Hyun Jung,Park, Hye Ji,Choi, Jin Hwa,Park, Do Youn Springer-Verlag 2013 Molecules and cells Vol.35 No.3

O⁶-alkylguanine-DNA alkyltransferase gene polymorphisms and the risk of primary lung cancer

Chae, Myung Hwa,Jang, Jin-Sung,Kang, Hyo-Gyoung,Park, Jae Hyung,Park, Jung Min,Lee, Won Kee,Kam, Sin,Lee, Eung Bae,Son, Ji-Woong,Park, Jae Yong Wiley Subscription Services, Inc., A Wiley Company 2006 Molecular Carcinogenesis Vol.45 No.4

<P>O<SUP>6</SUP>-alkylguanine-DNA alkyltransferase (AGT) plays an important role in the repair of O<SUP>6</SUP>-alkylguanine adducts, which are major mutagenic lesions produced by environmental carcinogens. Polymorphisms in the AGT gene may affect the capacity to repair DNA damage and thereby have influence on individual's susceptibility to smoking-related cancer. To test this hypothesis, we investigated the potential association of AGT polymorphisms (485C > A, Leu53Leu (C > T) and Leu84Phe] with the risk of lung cancer in a Korean population. The AGT genotypes were determined in 432 lung cancer patients and in 432 healthy controls who were frequency-matched for age and gender. The 485 AA genotype was associated with a significantly increased risk for overall lung cancer as compared with the 485 CC genotype and the combined 485 CC + CA genotype, respectively (adjusted odds ratio (OR) = 1.83, 95% confidence interval (CI) = 1.12–2.99, P = 0.02, and Bonferroni corrected P-value (Pc) = 0.04; and adjusted OR = 1.67, 95% CI = 1.05–2.66, P = 0.03, respectively). When the lung cancer cases were categorized by the tumor histology, the 485 AA genotype was associated with a significantly increased risk of adenocarcinoma (AC) and small cell carcinoma (SmCC), respectively, as compared with the combined 485 CC + CA genotype (adjusted OR = 2.54, 95% CI = 1.39–4.66, P = 0.003; and adjusted OR = 2.19, 95% CI = 1.06–4.55, P = 0.04, respectively). However, the genotype distributions of the Leu53Leu and Leu84Phe polymorphisms were not significantly different between the lung cancer cases and the controls. On a promoter assay, the 485C > A polymorphism did not have an effect on the promoter activity of the AGT gene. These results suggest that the effect of the AGT 485C > A polymorphism on the risk of lung cancer may be secondary to linkage disequilibrium (LD) with either another AGT variant or with a true susceptibility gene, and that the AGT 485C > A polymorphism could be used as a marker for the genetic susceptibility to lung cancer. © 2005 Wiley-Liss, Inc.</P>

모노아조 색소 탈색세균 Klebsiella pneumoniae DB51 의 특성

지원대,손동화,정현채,정민선,정영건,최웅규,최규양 한국위생과학회 1996 한국위생과학회지 Vol.2 No.1

On purpose to develop monoazo dye-decolorizing microorganism, DB51 which could highly decolorize acidic and reactive monoazo dyes was isolated and finally selected. The decolorization rates of this organism to two kinds of monoazo dyes were appeared as those of 74.5% to acid orange 6(AO6), 61.7% to acid orange 10(A010), 82.7% to reactive orange 16(R016) and 79.8% to reactive red 4(RR4) respectively. This isolate was identified as Klebsiella pneumoniae by using API 20E kit. On the temperature and pH effects to the growth rate of Klebsiella pneumoniae DB51 the most growths were appeared at the temperature of 30℃ and in pH 6.0. The growth of this isolate was inhibited an the media contained sodium chloride more than 3% of it. Effect of oxygen to the growth of Klebsiella pneumoniae DB51 was accelerated by more amount of oxygen volume. But, effect of oxygen to the decolorization rates by Klebsiella pneumoniae DB51 was decelerated by more amount of oxygen volume.

Comparison between predicted total digestible nutrients and actual total digestible nutrients using nutrient digestibility of rice straw and timothy in ruminants

Chae Hwa Ryu,Seul Lee,Byeonghyeon Kim,Sang Yun Ji,Hyunjung Jung,Hyun-Jeong Lee,Jae-Yong Song,Youl Chang Baek 충남대학교 농업과학연구소 2021 Korean Journal of Agricultural Science Vol.48 No.2

FOXO1 degradation via G9a-mediated methylation promotes cell proliferation in colon cancer

Chae, Yun-Cheol,Kim, Ji-Young,Park, Jin Woo,Kim, Kee-Beom,Oh, Hyein,Lee, Kyung-Hwa,Seo, Sang-Beom Oxford University Press 2019 Nucleic acids research Vol.47 No.4

<P><B>Abstract</B></P><P>Posttranslational modifications of the Forkhead family transcription factor, FOXO1, have been known to have important regulatory implications in its diverse activities. Several types of modifications of FOXO1, including acetylation, phosphorylation, and ubiquitination, have been reported. However, lysine methylation of FOXO1 has not yet been identified. Here, we reported that FOXO1 is methylated by G9a at K273 residue <I>in vitro</I> and <I>in vivo</I>. Methylation of FOXO1 by G9a increased interaction between FOXO1 and a specific E3 ligase, SKP2, and decreased FOXO1 protein stability. In addition, G9a expression was increased by insulin and resulted in insulin-mediated FOXO1 degradation by K273 methylation. Tissue array analysis indicated that G9a was overexpressed and FOXO1 levels decreased in human colon cancer. Cell proliferation assays revealed that G9a-mediated FOXO1 methylation increased colon cancer cell proliferation. Fluorescence-activated cell sorting (FACS) analysis indicated that apoptosis rates were higher in the presence of FOXO1 than in FOXO1 knock-out cells. Furthermore, we found that G9a protein levels were elevated and FOXO1 protein levels were decreased in human colon cancer patients tissue samples. Here, we report that G9a specific inhibitor, BIX-01294, can regulate cell proliferation and apoptosis by inhibiting G9a-mediated FOXO1 methylation.</P>

Non viral gene delivery to human ovarian cancer cells using arginine-grafted P AMAM dendrimer

Hwa Jeong, Lee,Soo Hyun, Jang,Su Jin, Choi,Ji Hyun, Oh,Song Wha, Chae,Kihoon, Nam,Jong Sang, Park 이화여자대학교 약학연구소 2012 藥學硏究論文集 Vol.- No.22

BACKGROUND: A specific and effective strategy is in demand to treat ovarian cancer successfully. Epidermal growth factor receptor (EGFR) is highly expressed in ovarian cancer, and thus EGFR antisense gene therapy can be a potential therapeutic strategy. METHOD: L-Arginine-grafted-polyamidoamine dendrimer (PAMAM-Arg) has been reported to be a novel nonviral gene delivery carrier. Therefore, the ability of PAMAM-Arg in transferring a luciferase gene to ovarian carcinoma SK-OV3 cells has been examined, and the cytotoxicity of the cationic polymer has been investigated. In addition, the suppression of cell proliferation has been evaluated by transferring an EGFR antisense gene to SK-OV3 cells using PAMAM-Arg. Polyethyleneimine (PEI) 25K was used as a positive control. RESULTS: As a result, in vitro gene transfection efficiency of PAMAM-Arg was enhanced with increasing transfection time and N/P ratios. PAMAM-Arg transferred the luciferase gene into cells more efficiently than PEI. In addition, PAMAM-Arg was minimally toxic to the cells whereas PEI 25K was highly toxic. The polyplexes formed by the EGFR antisense gene and PAMAM-Arg significantly reduced thymidine incorporation into the cells suggesting the suppression of cancer cell proliferation. CONCLUSION: These results suggest that a PAMAM-Arg/EGFR antisense gene complex can be used as a safe and efficient therapeutic agent for cancer gene therapy.

Thiophene-initiated polymeric artificial cathode-electrolyte interface for Ni-rich cathode material

Chae, Bum-Jin,Park, Jun Hwa,Song, Hye Ji,Jang, Seol Heui,Jung, Kwangeun,Park, Yeong Don,Yim, Taeeun Elsevier 2018 ELECTROCHIMICA ACTA Vol.290 No.-

<P><B>Abstract</B></P> <P>Ni-rich layered oxide (Ni-rich NCM) is a promising advanced cathode material for lithium-ion batteries (LIBs) because of its high specific capacity. However, the high Ni content in the layered structures is associated with poor surface stability, which in turn accelerates the capacity fading of the cell. To improve the interfacial stability of Ni-rich NCM cathode material, we propose poly(thiophene)-based artificial cathode-electrolyte interphase (CEI)-immobilized Ni-rich NCM cathode material, synthesized using a simple and convenient one-step spin-coating process. At room-temperature cycling, cells with thiophene-modified NCM811 cathodes exhibit improved cycling retention (>91.3%), compared those with bare NCM811 (84.2%). The effectiveness of the artificial CEI layer is evident during high-temperature cycling: cells with 36 kDa-NCM811 exhibit a specific capacity retention of 89.4%, whereas those with bare NCM811 exhibit a drastic decrease in the cycling retention capacity (55.1%) at 55 °C. XPS analyses reveal that the improved electrochemical performance of cells with cycled NCM811 cathodes is due to the surface stability of the cathode; the decomposed adducts in the recovered 36 kDa-NCM811 cathodes are less, whereas they are present in considerable amounts in the cycled NCM811 cathode. In addition, it is confirmed that the molecular weight of the polymer used for the artificial CEI-layer formation significantly affects the electrochemical performance of the cell; polymers with low molecular weights are preferred because P3HTs with low molecular weight have high crystallinity with a hard phase, particularly at high temperature, due to their low <I>T</I> <SUB>g</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Thiophene-initiated artificial CEI layer is immobilized on Ni-rich NCM cathode. </LI> <LI> Artificial CEI layer effectively suppresses electrolyte decomposition on electrode. </LI> <LI> NCM modified by artificial CEI layer gives improved high temperature cycling. </LI> </UL> </P>

Effects of 5-HT₄ selective receptor agonist, mosapride citrate on electrocardiogram in dogs

Chae, Ji Sang,Ahn, Jin Ok,Coh, Ye Rin,Park, Chong Woo,Youn, Hwa Young The Korean Society of Veterinary Science 2012 大韓獸醫學會誌 Vol.52 No.3

Mosapride stimulated dietary motility was introduced because of the arrhythmogenic effect of cisapride. Cisapride, 5-HT receptor agonist, induces prolongation of QT interval. Additionally, this condition can raise the possibility of acute, "malignant" arrhythmias such as torsade de pointes. It is hard to find any reports about effects of mosapride on cardiac parameters in dogs. By confirming electrocardiogram (ECG) parameters, the surface extremity leads ECG that was obtained from the four-limb electrodes and which was recorded by an ECG recorder after administration of mosapride 3 mg/kg PO b.i.d, and mosapride 3 mg/kg with itraconazole 5 mg/kg PO b.i.d, respectively. QT interval was shortened on the days of 3, 5, and post-day 1 in both mosapride 3 mg/kg administrated group and mosapride with itraconazole group. Heart rate increased significantly. QTc was slightly prolonged in mosapride administration group and mosapride with itraconazole group. However, all dogs of QTc were in normal variation (150~250 msec). Besides, the dogs showed no side effects reported in human medicine during the administration with these drugs. Although mosapride can increase the heart rate, this study suggest that mosapride may be useful for the dogs with disorders of gastrointestinal motility because of no fatal arrhythmogenic effect inspite of administration with itraconazole in dogs.