CRP* 의존성 maltose 대사 촉진 유전자 sfs4의 클로닝 및 염기배열 결정

최용락,정희태,조무제,정수열 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

CRP (cAMP receptor protein)은 cAMP와 결합하여 cAMP-CRP 복합체를 형성하여 전사조절의 조절인자로서 작용한다. crp 유전자에 변이를 도입하여 cAMP의 비존재 상태에서 cAMP-CRP와 비슷한 기능을 가진 crp* 유전자가 도입된 대장균 MK2001 (crp*¹, cya::km)을 숙주로 사용하여 cAMP 혹은 cGMP의 비존재하에서도 mal 유전자의 발현을 촉진시키는 유전자 sfs (sugar fermentation stimulation) 수 종을 클로닝하였다. 본 실험에서는 이미 밝혀진 nlp (Ner like protein) 유전자와 같이, sfs의 새로운 유전자를 탐색하여, 그 중 sfs4의 2126bp 전 염기배열을 결정하고, 잠정적인 sfs4의 promoter 영역에는 CRP 단백질과의 결합 DNA 공통 염기배열(5' AAT TGTGA ACACCA TCACC CGT 3')이 존재함을 확인했다. lacZ 융합 유전자를 작성하여 TP2010R1과 MK2001의 균주에서 cAMP를 첨가할 경우 각각 2.3배, 1.8배의 β-galactosidase 활성이 증가 하는 것으로 보아 sfs4는 cAMP-CRP에 의해 발현 조절을 받는 것으로 나타났다 In Escherichia coli, CRP forms a complex with cAMP and acts as a transcriptional regulator of many genes, including sugar metabolism operons. The E. coli MK2001, which is introduced the altered crp*¹, is functional in the expression of lac, ara and man, in the absence of cAMP. However, the expression of mal gene is fully activated by the addition of cAMP or cGMP. The object of the study is cloning of the sfs (sugar fermentation stimulation) genes, which was involved in regulation of mal gene expression with the altered crp*¹gene, and structural analysis and characterization of the genes at the molecular level. We have cloned 5 different E. coli genes which stimulate the maltose metabolism in a crp*¹, cya::km (MK2001) background. Newly identified genes were designated as sfs. One of the sfs genes (pPC1), located at the 53.2 min map position in the E. coli chromosome, was further analyzed. Expression of the genes, which is involved in maltose metabolism, malQ (amylomaltase), was increased to 5.8-fold in the presence of a plasmid, pAP5, containing the subcloned sfs4 gene. The nucleotide sequence of a common 2,126bp segment of the pPCM1 was determined and two open reading frames (ORF1 and ORF2) were detected. The ORF1 encodes the sfs4 gene and ORF2 encodes a truncated protein. Potential CRP binding site is located in the upstream of the putative promoter in the regulatory region. Expression of the cloned sfs4 gene was positively regulated by the cAMP-CRP complex.

韓國 東海沿岸海(注文津)에서 風成海流 影響下의 海洋汚染 樣相

崔孝,朴榮濟,崔俊 강릉대학교동해안지역연구소 1993 東海岸硏究 Vol.4 No.3

本 硏究에서는 東海의 注文津港口와 連谷川河口에서 沿岸海域으로 流入된 汚染水의 COD와 DO의 농도를 分析하여 沿岸海域에서의 汚染狀態를 調査하였다. 海水의 COD분포의 일반적인 樣相은 注文津 港口의 入口와 河川水의 流入이 많은 連谷川 河口에서 高濃度의 分布를 나타내며 沿岸에서 外海로 갈수록 低濃度의 分布樣相을 보여준다. 그러나 注文津港의 入口에서 海水가 低鹽分度를 나타내지만 COD의 濃度分布는 高濃度이고, 反面에 連谷川 河口에서는 다소 高鹽水이고 COD도 高濃度를 나타낸다. COD의 濃度가 漲潮流時에 注文津港의 入口에서 高濃度를, 落潮流時에 低濃度를 보여주며, 反對로 連谷川 河口에서는 漲潮流時에 低濃度를, 落潮流時에 高濃度를 보여준다. 外海로 갈수록 漲潮流時와 落潮流時에 큰 差異를 보이지 않는다. DO의 濃度分布는 汚染物質이 많이 排出되는 注文津 港口의 入口에서는 DO의 量이 減少함에 따라 海水의 汚染狀態가 심해감을 알 수 있으며 連谷川 河口 역시 生活汚水와 廢水의 放流에 依해 水質이 좋지 못함을 알 수 있다. 그러나 沿岸에서 外海로 갈수록 DO量이 增加하여 水質이 養好해져감을 볼 수 있다. 따라서 沿岸海域에서의 汚染狀態는 DO量이 가장 적은 注文津 港口의 入口가 가장 심하고 連谷川 河口에서 다소 덜 심하며 沿岸에서 3km밖의 外海側에서는 汚染이 되지 않은 海域임을 알 수 있다. By analyzing the concentrations of COD and DO of polluted waters flowed into the East Sea from the Chumunchin harbor and the Yunkok Stream estuary the pollution state in the coastal sea was investigated in this reseach. The general aspect of COD distribution of sea water showed that the concentrations were high in the mouth of the Chumunchin harbor and the eastuary of Yunkok Stream, which the fresh waters flowed in, but low in the open sea at the distance far away from the coast. In the mouth of Chumunchin harbor the salinity of sea water was low and the COD concentration was high, while in the eastuary of Yunkok Stream the salinity was also high and the COD was high. The distributions of COD in the mouth of Chumunchin harbor showed especially high concentrations during the flood, but low concentrations during the ebb. On the other hand, the COD concentrations in the estuary of Yunkok Stream were low for the flood and high for the ebb, but their concentrations in the open sea for both flood and ebb were not much different. Since the concentrations of DO decreased in the mouth of Chumunchin harbor, where a great amount of the pollutants were released, the pollution state became serious, and the water qualities in the estuary of the Yunkok Stream were also in the bad situation due to discharging sewage and waste water. However, the amount of DO increased as the distance from the coast to the open sea increased and the quality of sea water became in the good state. Thus, the pollution state in the coastal sea was worst in the mouth of Chumunchin harbor, where the concentration of DO was low, and relatively not much serious in the eastuary of the Yunkok Stream, but the sea the outside of 3km still contained clean waters.

Bochner 방법을 응용한 Shigella의 약제내성 제거

최정현,이제철,설성용 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

To study the optimal curing condition on selective medium described by Bochner et al. and modified medium, six strains of S. flexneri and 2 strains of S. sonnei were tested for minimum inhibitory concentration(MIC) of quinaldic acid and cured resistance pattern. Also, changing patterns of elimination according to incubation temperature were investigated at 37℃ and 42℃. MICs of Shigella on original Bochner medium and modified medium were 50 5o 400㎍/㎖. But MICs of Shigella were above of 400㎍/㎖ on the Bochner's medium excluding tetracycline and sodium phosphate. There were no differences in MIC between at 37℃ and 42℃. On the Bochner's medium, losses of drug resistance were more effective than other medium and cured resistance pattern was partial or total of original resistance, regardless of incubation temperature. Frequencies of curing were low on modified Bochner's medium, but most of cured resistance pattern were total resistance of Shigella. Frequencies of curing at 42℃ were similar to 37℃, but some strains showed resistance loss only at 42℃. MICs of 8 strains of Shigella were above of 400㎍/㎖ and no strains detected resistance loss on the both tryptic soy agar and tryptic soy broth.

외상에 이한 혈관 손상 환자에서 중재적 방사선 시술에 의한 치료

최지은,조용필,안제홍,한명식,장혁재,김용호,최윤백 대한혈관외과학회 2004 Vascular Specialist International Vol.20 No.1

유입량에 따른 빈도별 저수용량 결정에 관한 연구

최한규,최용묵,전광제 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A

A past monthly data is not faithful so much for a short term. But, the stochastic generation technique was provide of a long-term data. Thus this study is used a data which generated a monthly inflow amounts data by Thomas-Fiering model. This model is needed a certain process which determination of distribution, decision of continuous durability, etc. It was generated a inflow data every one month as Thomas-Fiering method. The generated inflow data was used input data for a monthly cumulative analysis. This analysis obtained a storage capacities which would be required during droughts having various return periods. It was presented a equation of fitting regression that carried out regression analysis of 5,10, 20, 50 years period.

WIPO의 전통지식 등의 지재권 설정 논의에 관한 개략적 방안 모색

이제현,김용진,최환수 대전대학교 한의학연구소 2004 혜화의학회지 Vol.12 No.2

The international government committee is progressing their agreements about intellectual property protection of traditional knowledge(TK1, gene resource(GR), folklore(FL) in WIPO. It is in the course of selection with precedence of TK, GR, FL in WIPO, focused on discussions about listing of TK documents, standardization of DB construction, sharing and profit distribution of GR. There are disagreements between developed countries and developing countries about intellectual property protection agreements of TK, GR. The developed countries insist on using the existing intellectual property protection, but the developing countries ask new ones on character of TK, GR. It causes intangible assets to be valuable trade properties in future world trade. This research Groped for a summary program about intellectual property protection of traditional knowledge(TK) etc. debating in WIPO. This program confirms that such as TK, GR etc. not only to be the cultural property accumulated in human history, but also to be the original resource may be using at present. Therefore, we suggest that the focus of discussion should transfer to UNESCO instead of WIPO which only deal with the intellectual property protection. Besides, the main body which protecting and supporting TK should become its holding organization so as to achieve more effective management about it. In order to protect and support TK, the government should have firstly the recognition that TK is the property belongs to the country. By this viewpoint, it needs to setup DB through overall excavation of the unofficial knowledges in order to protect and support these TK, GR. Because the positive dealing with those WIPO's discussion means to support our TK, GR, so there should have some reorganization about existing related ones, and also needs systemic supporting policies & management system.

우리 전통의약학 자료의 보호 방안 : WIPO의 전통지식에 관한 지적재산권 보호 논의의 대응 방안 모색 Finding ways against discussions of intellectual property protection on traditional knowledge by WIPO

이제현,김용진,최환수 대전대학교 한의학연구소 2004 한의학연구소 논문집 Vol.12 No.2

WIPO international government committee will finish to develop toolkit for making documentation on discussions of intellectual property protection with TK, GR until the late 2003. As they construct DB of TK, GR, it becomes in a hurry to construct DB of TK information in Korea. We looked into application of copyright in force and a lot of problems in terms of analyzing medical books in the resent condition or protecting them in Korea. There are 29 kinds which have been lost, 12 kinds which could have read in parts, 136 kinds which have been existed of Korean medical books before 1900. There are also a lot of medical books which have been published after 1900 and still are unpublished. It would be 406 medical books from China totally, 111 kinds from Chosun Dynasty version, 134 kinds from Euybangeuchui, 225 kinds from Hyangyackjibsungbang, 78 kinds from Dongeuybogam. We consider to know whether Korean medical books could been applied to the copyright in force or not in accordance with discussions of WIPO on the period, subject(author), contents of protection. If it is applied to the copyright in force, it will be decided to revise according to discussions of WIPO. On the other hand, the problem which is occurred to protect Korean medical books is friction with China as importing their medical books, to find our medical books actively which has been unpublished. Finally, We have to revise administration and intellectual property standards or protect our medical books by making new administration laws.

월경전기증후군의 심리적 요인에 관한 연구

이현제,최용성,권택술 大韓神經精神醫學會 1994 신경정신의학 Vol.33 No.2

Authors surveyed how the psychologic factors, especially the personality traits were related to the premenstrual syndrome, using PAF and MMPI, with 110 married nurses and 106 unmarried nursing students. The results were as follows : 1) There were no relations between the premenstrual syndrome and the demographic characteristics. 2) The high-scored types in PAF were turned out to be fatigue, lability, anxiety and atypical depressive features in rank order. On the contrary, the low-scored types in PAF were turned our to be increased well-being, hostility/anger, miscellaneous mood/behavior changes and impulsivity in rank order. 3) In the mean comparison of the MMPI scales among three clusters Pa, Pt and Sc scales showed statistically significant relations both in married and unmarried group, also D scale showed statistically significant relation in married group only and Hs, Ma scales showed statistically significant relation in unmarried group only. 4) In the mean comparison of the MPDS scales among three clusters BDL, ANT, CPS, PAG, PAR and STY scales showed statistically significant relations both in married and unmarried group, also DEP scale showed statistically significant relation in unmarried group only.

물리 시범실험 기구의 개발 및 물리교육에서의 활용

박용환,최제영 永同工科大學校 1996 硏究論叢 Vol.2 No.1

물리 시범실험 기구는 학생들의 학습 동기를 유발하고 물리 이론의 개념이나 원리, 법칙 등을 효과적으로 설명할 수 있는 장점이 있다. 본 연구에서는 자체 개발하거나 다른 문헌에 발표된 물리 시범실험 기구 20여종을 제작하여 물리 이론 교육에 활용하였다. 이러한 물리 시범실험 기구를 이용한 물리교육은 학생들의 물리 기본개념에 대한 정확한 이해를 유도하고 과학적 탐구정신을 계발하는데 큰 도움이 되었다. 또한 물리 실험의 실험기구 개발에 필요한 기초자료 및 타 전공에서의 시범실험 교육의 기초 자료로서 제공할 수 있다. Physics demonstrations are designed to motivate students' loaming and illuminate the concepts and principles of physics. In this work we developed and built 20 demonstration apparatus, some of which are well known.In-class demonstrations helped students understand basic concepts in physics and develope their scientific attitude. These apparatus may be used in developing experimental devices in physics and may be provided for helping develope demonstrations in other majors.

노인정신장애 평가척도(Psychogeriatric Assessment Scale)의 한국판 표준화 연구

조맹제,박임순,신영민,김무진,정희연,정은기,최용성,조성진,서국희,함봉진 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

연구목적 : 본 연구는 치매와 우울증을 다차원적으로 평가할 수 있는 도구인 Psychogeriatric Assessment Scale(PAS)의 한국판(PAS-K)을 개발하기 위한 것이다. 방 법 : 번역, 역번역, 그리고 세차례의 예비조사를 통하여 PAS-K를 제작하였다. 60세 이상의 노인 291명(임상환자군 : 58명, 지역사회노인군 : 168명, 수용시설 거주자 : 67명)과 그들의 정보제공자들을 대상으로 PAS-K, Geriatric Depression Scale(GDS), Korean version of Mini-Mental State Examination(MMSE-K), Hamilton Rating Scale for Depression(HRSD), 그리고 Hachinski Ischemic Score 를 적용하고, Diagnostic Interview chedule(DIS-Ⅲ-R)을 사용하여 치매와 우울증을 진단하였다. PAS-K의 신뢰도와 타당도를 평가하고, 예민도와 특이도, Kappa값, 그리고 ROC커브 분석법을 이용하여 PAS-K의 6개의 소척도들(피검자면접 : 인지기능 장애척도, 우울척도, 뇌졸중척도, 정보제공자면접 : 인지기능 저하척도, 행동변화척도, 뇌졸중척도)의 최적 절단점을 구하였다. 결 과 : PAS-K는 높은 내적 일관성을 보였고, 검사자간 신뢰도도 만족할 만한 수준이었다. PAS-K의 소척도들을 다른 표준화된 검사도구들과 비교하였을 때 인지기능 장애척도와 인지기능 저하척도는 MMSE-K와, 우울척도는 GDS 및 HRSD와 유의한 상관관계를 보였으며, 뇌졸중척도는 Hachinski Ischemic Score와 통계적으로 의미있는 상관관계를 보였다. 최적절단점은 인지기능 장애척도 10점, 인지기능 저하척도 3점, 우울척도 5점, 피검자면접과 정보제공자 뇌졸중척도 모두 1점, 그리고 행동변화척도 2점으로 추정하였다. 결 론 : 본 연구를 통해 PAS-K의 신뢰도와 타당도가 검증되었고, 노인정신의학 역학연구나 보건분야에서 치매와 우울증의 임상경과관찰 및 일차 선별도구로서 유용하게 사용될 수 있게 되었다. Objectives : This study was designed to develop the Korean version of the Psychogeriatric Assessment Scale(PAS-K), a multidimensional screening tool for the dementia and depression. Methods : Through three times preliminary trials, the authors translated PAS into Korean. The PAS-K, Geriatric Depression Scale, Hamilton Depression Rating Scale, Hachinski Ischemic Scale and Mini-Mental State Examination-Korean version were administered to 291 subjects over the age of 60 and the same number of their informants(67 subjects were institutionalized, 168 subjects were in community, 58 subjects were psychiatric hospital patients). And the Diagnostic Interview Schedule(DSM-Ⅲ-R) was independently administered to exactly diagnose dementia and depression. The reliability and validity test, optimal cut-off point estimation for six each scale and ROC curve analyses were done to investigate the diagnostic validity of PAS-K. Results : Internal consistency and interrater reliability of the PAS-K were high. Concurrent validity of each scales of PAS-K was good in being measured with other standardized scales. The optimal cut-off points of each scale of the PAS-K were estimated as follows : 1) Stroke Scale of subject interview : 1, 2) Cognitive Impairment Scale of subject interview: 10, 3) Depression Scale of subject interview : 5, 4) Stroke Scale of informant interview : 1, 5) Cognitive Decline Scale of informant interview : 3, and 6) Behavioral Change Scale of informant : 2. conclusions : The PAS-K was valid and reliable screening tools for detecting dementia and depression. Therefore the PAS-K could be widely and extensively used in psychogeriatric epidemiological research or clinical setting of primary screening for dementia and depression.