비육돈에 미생물제제 급여시 분뇨 특성에 미치는 효과

곽정훈,최동윤,박치호,김재환,정광화,양창범,유용희,천현식,라창식,Kwag, J.H.,Choi, D.Y.,Park, Ch.H.,Kim, J.H.,Jeong, K.H.,Yang, Ch.B.,Yoo, Y.H.,Chen, H.S.,La, C.S. 한국축산환경학회 2007 축산시설환경학회지 Vol.13 No.3

본시험은 비육돈사료에 미생물제제를 사료에 미생물제제 A 및 B 0.1 미생물제제 C를 0.2% 혼합 급여할 경우 사료섭취량 및 돈분의 오염물질 배설농도에 미치는 영향을 분석하기 위하여 4처리$\times$반복당 5두로서 총 20두를 공시하여 실시하였는데 그 결과를 요약하면 다음과 같다. 1. 비육돈의 일일 평균사료섭취량은 대조구 3.15 kg/일.두였고 미생물A, B, C구는 각각 3.14kg/일/두, 3.31, 3.42로 미생물제제 C구에서 일일 사료섭취량이 가장 높게 조사되었으며(p<0.05), 2. 일일평균 음수량은 사료섭취량이 높았던 미생물 C구에서 3.95kg/일/두로 가장 높게 조사되었다(p<0.05). 3. 미생물제제 처리구별로 분뇨배설량은 사료섭취량이 높았던 미생물제제 C구에서 가장 많이 배설되는 것으로 조사되었으며(p<0.05), 돈뇨의 배설량도 미생물제제 C구에서 2.23kg/일/두에서 높았다(p<0.05). 4. 돈분뇨의 수분 함량은 및 비료성분인 T-N, $P_{2}O_{5}$, $K_{2}O$ 성분도 처리 간에 큰 차이를 보이지 않았다(p<0.05). 5. 돈분뇨의 평균 BOD 농도는 돈분의 경우 미생물제제 B, C제제 급여구가 유의적으로 높게 조사되었다(p<0.05). 그리고 돈뇨의 BOD의 경우에는 대조구에서 $8,657.5mg/{\ell}$로 가장 높은 것으로 조사되었다(p<0.05). 6. COD 농도는 대조구에서 가장 높게 조사되었으며(p<0.05). 돈뇨의 경우에는 미생물제제 A급여구에서 평균 $9,545mg/{\ell}$로 가장 높았다(p<0.05). 7. SS 농도는 미생물제제 B급여구에서 가장 높게 조사되었으며(p<0.05), 돈분뇨중의 T-N 농도는 처리구간에 유의적인 차이가 나타나지 않았다(p<0.05). 그리고 T-P 농도의 경우에는 미생물제제 C급여구에서 유의적인 차이가 나는 것으로 조사되었다(p<0.05). 이상의 결과를 요약해보면 비육돈에 미생물제제 혼합급여시 사료섭취량과 음수량을 증가시키는데 효과가 있는 것으로 조사되었으나, 비료성분 배설량에는 큰 차이를 보이지 않는 것으로 조사되었으나, BOD 등 오염물질농도의 경우에는 미생물제제 A급여구에서 가장 낮게 조사되어 비육돈사료에 미생물제제 급여시 오염물질 저감효과가 있는 것으로 조사되었다. Study for the effect of three different microbial feed additives(henceforth MA-A, MA-B, and MA-C) on feed coversion rate, and physical and chemical characteristics of swine finisher was conducted. MA-B had higher number of Lactobacillus spp. and yeast, compared to any other. The amylase activity of MA-B was also higher than any other. The daily feed intake rates of pigs fed control, MA-A, MA-B and MA-C were 3.15, 3.14, 3.31 and 3.42 kg, respectively. MA-C had the highest weight gain. However, there was no significant difference between treatments. The weights of feces daily excreted by pigs fed control, MA-A, MA-B, and MA-C were 2.14, 2.02, 2.18, and 2.23 kg/day, respectively. The volume of urine daily excreted by pigs fed control, MA-A, MA-B, and MA-C were 3.14, 3.26, 3.27, and $3.41\;{\ell}/day$, respectively. Water content, T-N, $P_{2}O_{5}$, and $K_{2}O$ in swine manure were not significantly different between treatments. The BOD were between 42,576 and $67,450\;mg/{\ell}$ for feces and were between 5,882.5 and $8,657.5\;mg/{\ell}$ for urine, respectively. The SS were between 138,000 and $180,000\;mg/{\ell}$ for feces and were between 875.0 and $1450.0mg/{\ell}$ for urine, respectively.

산란간격을 단축시킨 오스트랄롭 선발계통에서 주요 조직적합성 복합(MHC)과 생산형질의 관련성

유병현,Sheldon, B. L. 한국축산학회 1998 한국축산학회지 Vol.40 No.5

Frequencies of three major histocompatibility complex (MHC) haplotypes (B^(8a) B^(9a) and B^(21)) segregating in three Australorp lines of the same origin were compared. Frequencies of B^(8a) and B^(9a) were higher in the AS line selected mainly for short oviposition interval than the AC line, a randombred control line. Frequencies of B^(9a) and B^(21) were higher in the ASS line, a commercial dam line derived from the AS line, than in the AS line itself. The differences in MHC haplotype frequency between the AS and AC tine were consistent with within-line estimates of the effect of the MHC on oviposition interval. However, the differences between the AS and ASS line could not be explained in terms of the effects of the MHC on the characters included in the selection criteria. It was suggested that interplay between a difference in susceptibility to avian leukosis virus (ALV) between MHC haplotypes and selection on a character affected by ALV infection could have been a contributing factor. The average effects of haplotype substitution (relative to B^(8a)) estimated within lines were consistent over the two selection lines for some characters (e.g. pooled estimates for oviposition interval were -0.11 h and 0.09 h for B^(9a) and B^(21), respectively), but different between the two lines for some other characters such as rate of lay, fertility and hatchability.

B-containing nanomaterial synthesis when a combustion wave moves within a packed bed of solid particles

Nersisyan, H.,Lee, T.H.,Yoo, B.U.,Kwon, S.C.,Suh, H.,Kim, J.G.,Lee, J.H. Elsevier [etc.] 2016 Combustion and Flame Vol.172 No.-

This study deals with combustion behavior of B<SUB>2</SUB>O<SUB>3</SUB>/Mg/NH<SUB>4</SUB>Cl/C complex systems for the synthesis of amorphous boron (B), boron carbide (B<SUB>4</SUB>C), and boron nitride (BN) nanostructures. The raw mixtures used in the experiments were prepared on the base of a B<SUB>2</SUB>O<SUB>3</SUB>-Mg precursor mixture, which is sufficiently exothermic to maintain a self-propagating regime of the combustion reaction. Thermodynamic analysis of the combustion temperatures and experimental validation indicate that the 1000-1500<SUP>o</SUP>C temperature range is very effective for synthesizing the nanostructures of B, B<SUB>4</SUB>C, and BN. It was found that B-containing functional nanostructures are mainly spherical nanoparticles (B) or nanosheets (B<SUB>4</SUB>C, BN). The phase composition and microstructural characteristics of the final products were evaluated based on the combustion temperature and solid/liquid phase changes.

22 H. pylori 감염시 NSAID의 항암 효과에 대한 실험적 증거

김도현 ( D. H. Kim ),이기명 ( K. M. Lee ),박현진 ( H. J. Park ),최찬임 ( C. I. Chor ),원명이 ( M. I. Won ),김인혜 ( I. H. Kim ),정재연 ( J. H. Jung ),유병무 ( B. M. Yoo ),김진홍 ( J. H. Kim ),조성원 ( S. W. Cho ),함기백 ( K. B. Hahm 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

腫瘍免疫療法 : 特히 胃癌을 中心으로

許景渤,金益洙,兪熙,閔珖植 順天鄕病院 1977 順天鄕醫報 Vol.2 No.1

Carcinoma is one of the most frequently observed malignant tumor in Korean patients. Result of surgery, for instance, in early stomach carcinomais encouraging and it is reported to be about 45% of five year survival rate but cases are few and rare. The majority of cases are that of either unresectable or metastatic regional lymphnode involvement of carcinoma. Their prognosis is poor. In those who had regional lymph node metastasis two year and three year survival rates are reported tobe each 37.1% and 17.9% respectively. Development of tumor immunology of the malignant disease made us greatly interested on the immunotheraly on the post resection stomach carcinoma. Studies on immunological competency by way of D.N.C.B. skin test started from 1971 and few cases those who may understand the new development of additional immuno therapy were selecter for B.C.G. innoculation after the subtotal gastrectomy. Recently monitering of peripheral thymus depentent lymphocytes (T-cell) count were undertaken to correlate the clinical course of the patients with the immunotherapy. In order to evaluate the immunological competency of healthy and all kind of malignant deseases of different stages, study with D.N.C.B. sensitization was undertaken in 264 cases. In healthy men of 53, only one man showed anergy, this anergic man is still healthy after three year observation. Table 1 further demonstrate the incedence of D.N.C.B. anergy in each group of patients, it notes that the unresectable malignant patients showed anergy 77 out of 105 cases(73.3%). From the D.N.C.B. sensirtization studies it may be clear to state that patient's immunological competency inthe malignant disease is severly deteriorated in the advanced tumo patients. The peripheral T-cell count also showed that the advanced malignant patients had lower T-cell count and it is progressively decreasing at the terminal stage(Table 2). In few cases who has B.C.G. after the subtotal gastrectomy the T-cell count are increasing after the treatment(Table 4). In 1971 five partients received B.C.G. therapy after the subtotal gastrectomy and three patients are sill living well. These 5 had all regional lymph node metastasis. The usual three year survival ratein similar patients is about 17 to 20%. Number of B.C.G. cases are not sufficient to a statistical significance but it may suggest a good deal of implication. Chance of two year survival after the B.C.G. therapy may be also far better than non-B.C.G.group(Table 5). The immunotherapy on malignant patients may be controversial, and yet we understand that the malignant tumor patients had impared immunity or immnological ability and that B.C.G. or immunotherapy may be effective on microscopic malignant lesion. In a selected case an immunotherapy after subtotal gastrectomy for gastric carcinoma may offer a beneficial effects on clinical course of the disease.

혈청 HBV bDNA 음성이나 간기능검사 이상을 보인 만성 B형 간염 환자들의 임상상

김상진 ( S. J. Kim ),박중원 ( J. Park ),김진희 ( J. H. Kim ),홍요한 ( Y. H. Hong ),정혜령 ( H. R. Jung ),김형준 ( H. J. Kim ),도재혁 ( J. H. Do ),유병철 ( B. C. Yoo ),박실무 ( S. M. Park ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> B형 간염바이러스(HBV)의 증식이 억제되면 만성 B형 간염의 활동성은 감소되며 이러한 이유에서 라미뷰딘과 같은 항바이러스제들을 치료제로 사용하고 있다. 그러나 HBV의 혈중 농도는 낮으나 간기능검사 이상을 보이는 만성 B형 간염 환자들이 상당수 있어 그 원인 규명이 필요한 실정이다. 이에 연구자들은 혈청 HBV branched DNA(bDNA) 음성인 만성 B형 간염 환자들을 대상으로 간 기능검사 이상과 관련될 수 있는 임상적 원인들을 조사하

Solanum nigrum produces nitric oxide via nuclear factor-&kgr;B activation in mouse peritoneal macrophages

An, H-J,Kwon, K-B,Cho, H-I,Seo, E-A,Ryu, D-G,Hwang, W-J,Yoo, S-J,Kim, Y-K,Hong, S-H,Kim, H-M Lippincott Williams Wilkins, Inc. 2005 EUROPEAN JOURNAL OF CANCER PREVENTION Vol.14 No.4

Nitric oxide (NO) is an antitumour molecule produced in activated macrophages and Solanum nigrum is a plant used in oriental medicine to treat tumours. In this study using mouse peritoneal macrophages, we have examined the mechanism by which Solanum nigrum regulates NO production. When Solanum nigrum was used in combination with 20 U/ml of recombinant interferon-&ggr; (rIFN-&ggr;), there was a marked cooperative induction of NO production. The increase in NO synthesis was reflected as an increased amount of inducible NO synthase (iNOS) protein. The production of NO from rIFN-&ggr; plus Solanum nigrum-stimulated peritoneal macrophages was decreased by treatment with N-monomethyl-L-arginine or N-tosyl-Phe chloromethyl ketone, an iNOS inhibitor. Additionally, the increased production of NO from rIFN-&ggr; plus Solanum nigrum-stimulated cells was almost completely inhibited by pretreatment with 100 μmol/l of pyrrolidine dithiocarbamate, an inhibitor of nuclear factor &kgr;B (NF-&kgr;B). Furthermore, Solanum nigrum increased activation of NF-&kgr;B. These findings suggest that Solanum nigrum increases the production of NO by rIFN-&ggr;-primed macrophages and NF-&kgr;B plays a critical role in mediating these effects.

Effect of the Length of Feed Withdrawal on Weight Loss, Yield and Meat Color of Broiler

Kim, D.H.,Yoo, Y.M.,Kim, S.H.,Jang, B.G.,Park, B.Y.,Cho, S.H.,Seong, P.N.,Hah, K.H.,Lee, J.M.,Kim, Y.K.,Hwang, I.H. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.1

The current study was conducted to determine the optimum length of feed withdrawal for pre-harvest broilers. A total of three hundred broilers were sampled from an industrial population, and 30 chicks for each weight group (e.g., 1.5 and 2.5 kg) were randomly assigned to feed withdrawal treatments for 0, 3, 6, 9 and 12 h. Weight loss, yield, muscle pH, objective meat color and weights of gastro intestinal contents, crop, gizzard, provenriculus, small intestine, caecum, and rectum were determined. Live weight loss was significantly (p<0.05) increased as length of feed withdrawal extended. A significant (p<0.05) carcass yield for both 1.5 and 2.5 kg groups coincided after 9 and 6 h feed withdrawal, respectively. Net weights of intestinal contents for crop and gizzard were significantly (p<0.05) reduced by 6 h, and the reduction for proventriculus and small intestine occurred from 3 h. A noticeable effect of feed withdrawal on pH for breast muscle at 3 h postmortem occurred only when chicks were fasted for 3 h of which pH (6.05) was significantly (p<0.05) higher than that for other groups including the control (5.74). There was a linear tendency of higher lightness (Hunter L* value) numerically for chicks fasted for longer periods. The highest coefficient of determinations of regression models to estimate weight loss as a function of fasting period and body weights were achieved, when the models included both linear and quadratic terms for fasting period, and linear term for both 1.5 ($R^2=0.76$) and 2.5 kg ($R^2=0.78$) body weight groups. Given the practical aspect, approximately 1.5 kg of body weight is dominant, weight loss could be predicted by the following function; live weight $loss=26.6-0.28{\times}(fasting period)^2+12.34{\times}pasting\;period-0.012{\times}body\;weight$, $R^2=0.76$. Current data implied that the optimum fasting time for pre-slaughter chicks varied depending on slaughter weight; 6 and 9-h fasting were recommendable for 2.5 and 1.5 kg chicks, with little effect on objective meat color.

과산화수소가 함유된 저산도 질산용액에서 DEHPA 추출제에 의한 Np 의 추출거동

이일희,양한범,김광욱,임재관,유재형 ( E . H . Lee,H . B . Yang,K . K . Kim,J . K . Lim,J . H . Yoo ) 한국공업화학회 1996 공업화학 Vol.7 No.4

본 연구는 Np의 환원제로 H₂O₂가 함유된 IM 이하의 저산도 질산용액으로부터 DEHPA(di-(2-ethyhexyl)phosphoric acid) 추출제에 의한 Np의 추출 및 역추출 조건 설정과 추출속도 향상에 주안점을 두어, 회분식으로 실험을 수행하였다. 저산도 질산용액에서 Np의 산화상태는 주로 Np(V)로 존재하고 있음을 확인하였으며, NP의 추출율은 H₂0₂농도 및 DEHPA의 농도 증가에 따라 증가하고, 질산농도 증가에 따라 급격히 감소하였다. 제3의 산화/환원제가 첨가되지 않는 경우 추출율은 약 70% 정도로 다소 낮지만, DEHPA에 의해 추출이 가능함을 보았다. 또한 추출속도는 H₂O₂농도의 0.516 승에 비례하며, 질산농도의 0.483 승에 반비례하고 있는 다음과 같은 식을 얻었다. d[Np(V)]/dt=-1.391×10^(-2)[H₂O₂]^(0.516)[HNO₃]^(-0.483) [Np(V)] 그리고 과산화수소의 첨가 유무에 관계없이, 유기상으로 추출된 Np은 옥살산(oxalic acid)에 의해 효과적으로 역추출되었으며, 0.5M 옥살산으로 약 92% 이상을 역추출하였다. Extraction behaviour of Np with DEHPA(di-(2-ethyhexyl) phosphoric acid) from the low nitric acid solution(below 1M HNO₃) containing H₂O₂as a reducing agent was studied at a batch system in order to establish the conditions of extraction and stripping and to enhance the extraction rate. As results, it was confirmed that the Np was mainly the pentavalent oxidation state in the low nitric acid solution. The extraction yield of Np was increased with increasing the concentration of DEHPA and H₂O₂and decreased more rapidly with the increase of HNO₃ concentration. It was also found that the Np could be extracted into DEHPA even without the addition any redox agents, although the extraction yield is rather low as about 70%. The extraction rate was proportional to the 0.516 power of H₂O₂concentration and inversely proportional to 0.483 power of HNO, concentration as follows. d[Np(V)]/dt=-1.391×10^(-2)[H₂O₂]^(0.516)[HNO₃]^(-0.483) [Np(V)] Regardless of the H₂O₂, the Np extracted in the organic phase was effectively stripped to the aqueous phase with H₂C₂O₄. The Np could be stripped more than 92% with 0.5M H₂C₂O₄.

혈액검체로부터 분리된 황색포도구균의 독소유전자형 및 PFGE 분석

이영선,차정옥,정영희,최연화,양숙자,김홍빈,유재일,김봉수 국립보건연구원 2000 국립보건원보 Vol.37 No.-