인삼고본환과 그 구성약물군의 항산화 효과

뢰영성 ( Yong Sung Loi ),권영모 ( Kwon Yeong Mo ),박종흠 ( Park Jong Heum ),박선동 ( Park Seon Dong ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.3

N/A objectives: Exposure to oxidant molecules can generate reactive oxigen species(R0S; for example, H₂O₂, O₂-, OH .). These free radicals can alter DNA, proteins and membrane phospholipids. ROS are responsible for aging and many diseases including cancer, neurodegenerative diseases and cardiovascular diseases. Thus, to prevent against celluar damages associated with oxidative stress, antioxidants are important and many research about antioxidants are being conducted over the world. methods: This study was performed to research the antioxidant activities of Insamgobonwhan(IG) and its component groups in vitro, so intended to know the possibility of Insamgobonwhan(1G) as drug which can prevent aging. The experimental group was divided into 4 groups: Imsamgobonwhan(IG), two dong-two jiwhang(IG-1; 麥門冬, 天門冬, 熱地黃, 生地黃), two dong(IG-2; 麥門冬, 天門冬) and two jiwhang(IG-3; 熱地黃, 生地黃). results: 1. All experimental groups had significant antioxidant activity in linoleic acid system on concentration dose--dependently in comparison with control group. But IG and IG-2 have more effect than IG-1 and IG-3 relatively. 2. DPPH radical scavenging experiment and inhibitory effect experiment on superoxide generation in xanthine-xanthine oxidase system showed that all experimental groups had a dose-dependent effect. But IG and IG--1 had more effect than IG-2 and IG-3. 3. Inhibitory effect experiment on superoxide generation induced by xanthine-xanthine oxidase system showed that all experimental groups had a dose-dependent effect. But IG and IG-1 had more effect than IG-2 and IG-3. 4. Inhibitory effect experiment on lipid peroxidation reaction in H₂O₂-Fe²system showed that all experimental groups had a dose--dependent effect. But IG and IG--1 had more effect than IG-2 and IG-3. 5. Experiment on lipid peroxidation removal effect showed that all experimental groups had a dose-dependent effect. But IG and IG-2 have more effect than IG-1 and IG-3. 6. Experiment on glutathion activity effect showed that all experimental groups had some effect. But each groups had no significant difference. 7. Experiment on catalase activity effect showed that all experimental groups had some effect. But each groups had no significant difference. conclusion: These results showed that IG has an antioxidant activity, so it seems to have some anti-aging effect. From comparisons of each experimental groups, IG`s antioxidant effect is likely to be associated with IG-1 mostly.

제조(제조)로부터 혈전분해효소 45,000 dalton 의 정제와 그 특성에 관한 연구

朴賢俊,朴宣東 대한본초학회 1998 大韓本草學會誌 Vol.13 No.1

돌연변이원성 카르보닐화합물에 대한 단백질 가수분해물의 돌연변이원성 억제작용

박영호,염동민,김선봉 한국노화학회 1992 한국노화학회지 Vol.2 No.1

3-Chloromethyl-3-Methyl Oxetane의 합성

박정환,한도수,허선아,조성동 조선대학교 기초과학연구소 1993 自然科學硏究 Vol.16 No.1

3-chloromethyl-3-methyl oxetane(CMMO) was synthesized with 3-methy-3-methoxytosyl oxetane(MMTO) and LiCI in 68% yield.

펄스 코로나 방전 반응기를 이용한 NOx 제거 효율 및 입자 성장 분석

박정환,김동주,김교선 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

In this study, we analyzed the NOx removal efficiency and particle size distribution by the pulsed corona discharge process and investigated the effect of several process variables. The NO removal efficiencies and the particle characteristics were measured and analyzed as the function of initial concentrations of NO, H2O and NH3, applied voltage, pulse frequency and residence time. As the frequency of applied voltage increases, or as the applied voltage increases or as the residence time increases, the NO removal efficiency increases. The change of initial NH3 and H2O concentrations do not affect the NO removal efficiency significantly. The particle concentration and size increases with the increases of initial NO concentration, residence time and applied voltage.

Dong-Chun Lecture : Cell-based High Content Screening (HCS) for action modes of drug targets by using systematic deletion mutants of Schizosaccharomyces pombe

( Dong Uk Kim ),( Im Seon Woo ),( Mi Sun Won ),( Young Joo Jang ),( Kyung Sook Chung ),( Han Oh Park ),( Hyang Sook Yoo ),( Kwang Lae Hoe ),( Doo Sik Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

저온 플라즈마 공정에 의한 NO_x 제거시 공정 변수의 영향

박정환,김동주,김교선 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

In this study, we analyzed the NOx removal effiency by the low temperature plasma process and investigated the effect of several process variables. Most of NO is converted into NO2 and, later, into HNO3 which reacts with NH3 to form NH4NO3 particles. As the frequency of appling voltage increases, as the applied voltage increases or as the residential time increases, removal efficiency of the NO supplied initially increase. The removal efficiency of NOx also increases with the increase of NH3 supplied.

E. coli 과량발현을 통한 B형 간염 바이러스 preS1 유도체의 안정한 동위원소 N의 치환

박병관,김세하,한규훈,김선영,홍요정,이시형,한동설,김순종,박진구 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

We have used molecular biology technique to label the side chains and backbone of HBV preS1(1-56) with stable isotope (??) during E. coli over-expression and purified the protein to near homogeneity. The expression level of ?? labelled preS1(1-56) seems to be ∼15% of the total protein. to characterize the secondary structure of the labelled protein, circular dichroism was used. In different buffer conditions including pH 3.3 and pH 7.4 the protein seems to be devoid of any stable secondary structure probably suggesting that the N-terminal portion (preS1(1-56)) of HBV preS1 might be unstructured before binding to its target cells.

아동의 눈-사지 협응과 물체조작 기술의 관계

김선진,한동욱,박승하,김용호 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.2

본 연구의 목적은 아동의 눈-사지 협응 능력과 물체조작 기술의 차이를 알아보고, 두 변인군의 관계를 구명하는 데 있다. 피험자는 남?여 5-10세의 아동 186명을 대상으로 하였다. 눈-사지 협응 능력은 예측 타이밍 능력, 추적 능력, 전신 반응 능력을 측정하여 분석하였으며, 물체조작 기술은 TGMD(Test of Gross Motor Development)에서 제시한 받기, 던지기, 치기, 차기, 튀기기 기술을 평가하였다. 먼저 눈-사지 협응 능력과 물체조작 기술의 연령과 성별에 따른 특성을 알아보기 위해 이원 변량분석을 실시한 결과, 5세의 아동 집단은 전반적으로 수행능력이 6세, 7세, 8세, 9세, 10세의 아동에 비하여 수행능력이 떨어졌으며, 10세의 아동은 다른 연령 집단에 비하여 우수한 수행능력을 나타냈다. 이러한 결과는 5-6세, 9-10세의 연령 시기가 눈-사지 협응 능력과 물체조작 기술이 변화되는 시기임을 나타내주고 있으며 이는 연령에 따른 특징을 보여주는 것이다. 다만 눈-사지 협응 능력과 다르게 물체조작 기술에 있어서는 성별에 따른 수행 능력이 9세와 10세의 연령 집단에 있어서 상반된 특징이 나타났다. 즉 남자 아이들의 경우, 9세의 연령 집단과 비교하여 10세의 연령 집단은 물체조작 기술의 수행 능력이 급격하게 증가한 반면에 여자 아이들은 수행 능력이 떨어지는 특징을 보였다. 또한 눈-사지 협응과 물체 조작 기술간의 관계를 알아보기 위해 정준 상관분석을 실시한 결과, 두 과제간 상관계수 .599로 통계적으로 유의한 상관관계를 보였다. 이러한 결과를 바탕으로 눈-사지 협응 능력과 물체조작 기술의 관계를 설명해 주는 각 변인들의 상대적 기여도를 살펴보면, 눈-사지 협응 능력에서 손과 발을 사용한 예측 타이밍 능력이 추적 및 전신 반응 능력보다 높은 기여도를 보였다. 또한 물체조작 기술에 있어서는 받기와 튀기기 능력이 눈-사지 협응 능력과 물체조작 기술의 상관관계를 설명하는데 많은 기여를 하는 것으로 나타났다. The purpose of this study was to investigate the difference of eye-limb coordination and the object-control skills with age and sex, and examine the relationship between two-variable groups. For this experiment, 186 children with the age of 5 to 10(99 boys and 87 girls) participated in the study. The eye-limb coordination ability(anticipation timing ability, pursuit ability and reaction ability) and the object-control skill(catching skill, throwing skill, striking skill, kicking skill, bouncing skill) suggested in TGMD(Test of Gross Motor Development)were tested. The two-way ANOVA was used to present the characteristics of the eye-limb coordination ability and the object-control skills according to age and sex. As a result, children with the age of 5 demonstrated the lowest performance while children with the age of 10 showed high performance significantly among age groups. The children with the age of 5 to 6 and 9 to 10 were found to be two critical periods of change with age. However, unlike the eye-limb coordination ability, there was a distinctive feature in the object-control skills of the age 9 and 10 over sex. 10 year-old boys showed a radical progress, but the 10 year-old girls showed a decline on the object-control skills compared with the 9 year-old. A canonical correlation analysis was used to examie the relationship between the eye-limb coordination and the object-control skills for the early childhood. The relationship revealed significantly showing canonical correlation with coefficient .599. Overall, the results indicated the eye-limb coordination ability such as anticipation timing ability with hand and foot showed higher role than pursuit and reaction ability. In addition, the object control skills such as catching and bouncing ability contributed importantly in explaining the relationship between the eye-limb coordination ability and the object control skill.

카드뮴이 인간 단핵구 세포주(THP-1)에서 cytokine 유전자 발현에 미치는 영향

신동훈,박관규,강미정,서석권,정인성,양선희 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.2

Cadmium, a potent toxic metal, posses a serious environmental threat but the mechanism of its toxicity remains unclear. Also, cadmium is a known immunotoxic agent in animal studies and induces pathophysiological effects by modulating components of immune system. Cytokines are being increasingly recognized as essential mediators of normal and pathologic immune response. Cells of mononuclear phagocytic system are strategically located at portals of entry in humans and therefore may be particularly at risk for cadmium exposure through contaminated air, food, and drinking water. In the present study, we investigated the effect of cadmium cytotoxicity for the monocyte and expression of cytokine gene in the control and cadmium treated human monocytic cell lines using RT-PCR method. The results showed that cadmium inhibited cell proliferation at 0.1mM cadimium treated cells for 24 hours. The TNF-α mRNA was expressed in both control and cadmium treated cells but not IL-6 and IL-1β. The mRNA levels of TNF-αwere examined during 24 hours culture period, at different time points. The expression of TNF-αmRNA increased in both 0.01mM and 0.1mM cadmium treated cells, but did not show dose-response relationship. According to cadimium treated duration, expression of TNF-αmRNA was more decreased in 24 hours than 6 hours. The decreased levels of mRNA of TNF-α suggest that cadmium suppresses its production at the transcription level.