C형 간염바이러스 혈증 검출을 위한 총중심항원과 RNA 검사 비교

박도심,이영진,이기은,조지현,김학철,전창덕,곽병희 대한진단검사의학회 2002 Annals of Laboratory Medicine Vol.22 No.6

Background : Recently, trak-C (Total HCV core antigen test; Ortho Clinical Diagnostics, Raritan, NJ, USA) that is based on the enzyme-linked immnunosorbent assay was developed. So, we tried to compare the performance of the Hepatitis C virus (HCV) total core antigen test (HCVAg) with the qualitative in-house reverse transcription (RT)-polymerase chain reaction (PCR) assay and HCV-RNA Quantitation assay (HCVQn, Amplicor HCV monitor test version 2.0; Roche Diagnostics System, Basel, Switzerland). Methods : Dilution test was performed to evaluate the reproducibility and detection sensitivity of the methods. 87 sera of 70 Hepatitis C virus antibody (Ab) positive patients were tested to evaluate the diagnostic sensitivity and concordance of three methods, and to discover the quantity relationship of HCVAg and HCVQn. We also contained the results of 100 negative control specimens by HCV Ag to evaluate the specificity. Results : In the dilution test, the coefficient variation values of HCVAg were 41%, 29%, and 21% and HCVQn were 17%, 11%, and 150% of diluted sera respectively at 50, 5-1, and 5-2 dilution factor for four days running. The qualitative in-house HCV RT-PCR (5-5 dilution factor) and HCVQn (5-5 dilution factor) were more sensitive than the HCVAg (5-2 dilution factor). And the diagnostic sensitivity was high in order on the qualitative in-house HCV RT-PCR; 97%, HCVQn; 91%, and HCVAg; 85%. The concordance rate between the three methods was 83.9%. The quantity of HCVAg and HCVQn showed a significant correlation (R=0.8, P<0.0001). Conclusions : HCVAg showed reliable results and a significant correlation with the quantitative RNA level. HCVQn showed a quantitative result but less sensitivity than the qualitative in-house HCV RT-PCR. Even though the HCVAg has slightly lower sensitivity than the two methods, methodologically, it is the most cost-effective, is simple, and gives high throughput. So it should be a simple economic surrogate marker for viremia detection and viral load monitoring. (Korean J Lab Meb 2002; 22: 431-6)

향균제 사용과 접종 혈액량에 따른 호기성 BacT/Alert혈액형의 양성률 평가

박도심,이영진,유수진,조지현 대한임상병리학회 2001 대한임상병리학회지 Vol.21 No.5

자동화학발광 면역분석기 Immulite 2000에 대한 평가

박도심,이영진,조지현 대한임상병리학회 2000 대한임상병리학회지 Vol.20 No.5

라미부딘 치료 환자에서 Elecsys 2010 면역분석기를 이용한 B형 간염바이러스 e항원 반정량 검사의 유용성

박도심,이영진,조지현,최복희 대한진단검사의학회 2003 Annals of Laboratory Medicine Vol.23 No.1

배경 : HBV DNA 정량검사는 B 형간염바이러스 증식상태와항바이러스 치료효과 검사로 흔히 사용되나 복잡한 수동식 검사로 결과 보고가 늦다. 반면 HBe항원 검사는 간편하고 빠르나 정성 검사이므로 질환의 경과 관찰에 있어 임상적 유용성이 적다.Elecsys 2010 면역분석기(Elecsys 2010; Roche DiagnosticsGmbH, Germany)를 이용한 HBe항원 검사는 반정량이 가능하다. 이에 저자들은 Elecsys 2010에서 HBe항원 반정량 검사의 임상적 유용성을 평가하고자 하였다.방법 : 41명의 HBe항원, HBV DNA 양성 환자에 대해 18개월 동안 HBV DNA와 HBe항원의 결과를 비교하여 전체 검체(n=287)의 HBe항원과 HBV DNA의 상호 상관성을 검토하였고, 라미부딘 치료(n=30, 12-18개월) 후 HBe항원과 HBVDNA의 변화를 비교하였다.결과 : HBe항원과 HBV DNA는 양의 상관관계 보였다(R=0.56, P<0.05). 라미부딘 치료 2, 5개월의 HBe항원 농도와 감소율은 HBV DNA 음전화군(2군, 3군)과 HBV DNA 잔존군(4군) 사이에 현저한 차이( P<0.05)를 보였다. HBV DNA 음전화군에서 HBe항원과 HBV DNA의 농도와 감소율은 유사한 변화를 보인 반면, HBV DNA 잔존군은 HBV DNA 농도 변화나높은 감소율에 비해 HBe항원의 변화는 현저히 지연되어 있었다.

외상후 저산소성 간손상과 동방괸 용혈성 요독증후군 1예

박도심,이영진,조지현 한국혈전지혈학회 2000 한국혈전지혈학회지 Vol.7 No.2

Diagnostic Value and Prognostic Significance of Pleural C-Reactive Protein in Lung Cancer Patients with Malignant Pleural Effusions

박도심,황기은,황유리,박철,설창환,조경화,김병윤,박성훈,정은택,김학렬 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.2

Purpose: C-reactive protein (CRP) has been implicated in various inflammatory and advanced malignant states. Increased serum CRP (s-CRP) levels have been shown to be associated with independent prognostic factors for survival in patients with advanced lung cancer. However, only few studies have focused on the role of CRP in pleural effusions. This study aimed to evaluate the diagnostic and prognostic value of pleural CRP (p-CRP) in lung cancer patients with malignant pleural effusion (MPE). Materials and Methods: Pleural effusion (PE) samples were collected from patients with MPE (68 lung cancers; 12 extrathoracic tumors), and from 68 patients with various benign conditions (31 with pneumonia; 37 with tuberculosis). Concentrations of p- and s-CRP were measured by enzyme-linked immunosorbent assay. CRP level in pleural fluid and its association with survival were examined. Results: p-CRP levels correlated with s-CRP levels (r=0.82, p<0.0001). For the differential diagnosis of MPE and benign PE, the area under the receiver operating characteristic curve was greater for p-CRP (0.86) than for s-CRP (0.77). High p-CRP expression significantly correlated with shorter overall survival (p=0.006). P-CRP was independent prognostic factor significantly associated with overall survival on multivariated analysis (p=0.0001). The relative risk of death for lung cancer patients with high p-CRP levels was 3.909 (95% confidence interval, 2.000-7.639). Conclusion: P-CRP is superior to s-CRP in determining pleural fluid etiology. Quantitative measurement of p-CRP might be a useful complementary diagnostic and prognostic test for lung cancer patients with MPE.

통년성 알레르기비염 환자에서 혈장 MCP-1, RANTES, IL-18 농도

박도심,이재훈 대한이비인후과학회 2007 대한이비인후과학회지 두경부외과학 Vol.50 No.12

Background and Objectives:Plasma levels of MCP-1, RANTES and IL-18 are related to allergic diseases such as atopic der-of these cytokines and allergic rhinitis. Subjects and Method:The concentrations of plasma MCP-1, RANTES and IL-18 were analyzed by ELISA. The levels of cytokines were compared betwen perenial allergic rhinitis patients (n= 48) and controls (n= 33), and this depended on symptom severity. Results:Plasma levels of MCP-1 and IL-18 were significantly increased more in allergic rhinitis patients (MCP-1, 108 pg/mL, p<0.01;IL-18, 299 pg/mL, p<0.01) than in controls (MCP-1, 86 pg/mL;IL-18, 168 pg/mL). Especially, the levels of moderate-severe allergic rhinitis group (MCP-1, 116 pg/mL;IL-18, 329 pg/mL) showed higher tendency than mild one (MCP-1, 101 pg/mL;IL-18, 273 pg/mL). However, the levels of RANTES did not exhibit any diferences betwen allergic rhinitis and control. Conclusion:Our results suggest that plasma MCP-1 and IL-18 might be asso-(Korean J Otorhino -laryngol-Head Neck Surg 2007 ;50 :1103-8)

지속성 알레르기비염 환자에서 혈액 내 IL-31, Stem Cell Factor, B Cell-Activating Factor 농도

박도심,정하민,이상헌,이재훈 대한비과학회 2009 Journal of rhinology Vol.16 No.1

Background and Objectives:Stem cell factor (SCF), B cell-activating factor (BAFF) and Interleukin (IL)-31 are related to allergic diseases such as atopic dermatitis or asthma. But they have not been sufficiently investigated in connection with IgEmediated allergic rhinitis. In this study, we evaluated the association between plasma concentrations of these cytokines and allergic rhinitis. Subjects and Methods:A comparative study of the concentrations of plasma SCF, BAFF and IL-31 were conducted between persistent allergic rhinitis patients and a healthy control group using ELISA. Results:Plasma BAFF and IL-31 concentrations were significantly increased in allergic rhinitis group (BAFF, 1,255 pg/mL, Plasma BAFF and IL-31 concentrations were significantly increased in the allergic rhinitis group (BAFF, 1,255 pg/mL, p<0.001;IL-31, 221 pg/mL, p<0.001) than in the control group (BAFF, 1,089 pg/mL;IL-31, 153 pg/mL). But the level of SCF did not exhibit any difference between the allergic rhinitis patients and the control group. Conclusion:Our results suggest that plasma BAFF and IL-31 might be associated with the inflammatory mechanism of persistent allergic rhinitis patients.

알레르기비염 환자의 혈청 및 비세척액에서 YKL-40의 특이 표지자로서의 유용성

박도심,이영진,임준,유상우,남기성,이재훈 대한이비인후과학회 2012 대한이비인후과학회지 두경부외과학 Vol.55 No.3

Background and Objectives Chitin is a recognition element for tissue infiltration by innate cells implicated in allergy and asthma. Chitinases are characterized by the ability to cleave chitin. YKL-40, the chitinase-like protein, was increased during Th2-type inflammation in an exaggerated manner in tissues of patients with asthma. However, the relationship of YKL-40 level to allergic rhinitis has not been evaluated. Hence, we evaluated the relationship between the YKL-40 level in the blood and nasal lavage fluid and allergic inflammation in nasal cavity. We also evaluated the nature of association between several important factors (eosinophil count and total IgE) in the blood and nasal lavage fluid of allergic rhinitis patients. Subjects and Method The concentrations of the YKL-40 levels in the blood and nasal lavage fluid were compared between allergic rhinitis patients and healthy controls. We evaluated the YKL-40 levels in the blood and nasal lavage fluid and also evaluated symptom severity, eosinophil count, and total IgE. Results The blood YKL-40 level was not significantly increased in allergic rhinitis (49 pg/mL) than in control (44 pg/mL)(p>0.05). The YKL-40 levels in the nasal lavage fluid was not significantly increased in allergic rhinitis (1568 pg/mL) than in control (1248 pg/mL)(p>0.05). The YKL-40 levels in blood and nasal lavage fluid were not associated with important factors such as symptom severity, eosinophil count, and total IgE in allergic rhinitis patients. Conclusion There is no association between the YKL-40 level in the blood and nasal lavage fluid, allergic inflammation in nasal cavity.

병원에서 분리된 녹농균에 대한 역학적 조사 - 집락형, 항균제 내성 및 Pulsed - Field Gel Electrophoresis 분석-

박도심,조지현,이영진,문영회 대한임상병리학회 1999 대한임상병리학회지 Vol.19 No.6