세균 분류에 있어서 DNA 영동상의 수치분석방법에 대하여

문영회,조지현,전해은 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2

Microbial taxonomy used to be based on morphological and physiological features. Recently the classical phenotypical characterization of bacteria is being increasingly replaced by numerical taxonomy of electrophoretic separation technique of cellular proteins, fatty acids and DNA restriction fragments. We developed ‘correcno. prg’ to standardize electrophoretic DNA patterns with varying mobility. As a model system, DNA size markers, plasmids of E. coli and their pstl digests, were electrophoresed on 28 lanes of 3 gels(0.7% agarose, 0.5μg/㎖ Ethidium bromide) for varying times(1.5 hour to 5 hour including 77 lanes). Also chromosomal DNA of V. cholerae and their Hind Ⅲ and EcoR1 digests, were electrophoresed for 4 hour 20 minutes. Each gel was photographed, scanned and digitalized. The numeric data of each lane was analyzed to construct similarity matrix of Pearson product-moment coefficient, and clustered by UPGMA(unweighted pair-group method using arithmetic averages) method. The clustering results were useful except indistinct smearing DNA.

Porphyria의 진단 - [1] 급성간성 폴피리아의 진단에 대하여

문영회,허숙조 대한임상병리학회 1981 대한임상병리학회지 Vol.1 No.1

임신중 뇨-에스트리올 배설량과 알카리성 인산염효소치와의 비교검토

문영회 中央醫學社 1974 中央醫學 Vol.26 No.1

The serum heat stable alkaline phosphatase (S-HSAP) was assayed to measure only the placental iso-enzymes in a group of normal pregnant women in order to establish the normal range. Ninety four paired estimations of serum heat stable alkaline phosphatase and urinary estrogen excretion were performed on normal or patients with complication of pregnancy. Serum-HSAP determinations were found to be of no use in predicting fetal -distress of fetal dysmaturity. Serial assays of urinary estrogen output enabled dysmaturity to be correctly predicted in 63.7 percent of cases, but there was also an incidence of 18.2 percent false positive predictions. There was no correlation between the serum HSAP and fetal weight, and urinary estrogen output and placental weight, although there are correlations between the serum HSAP and placental weight or urinary estrogen output and fetal weights. Urinary estrogen assays were less reliable in predicting fetal distress and Serum HSAP levels are probably of no value in the prediction of a small-for-dates baby.

인간 융모 성선자극홀몬 (hCG) 에 의한 생쥐의 뇌 소교세포에서 산화질소 (NO) 의 생성

문영회,민부기,김형민 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.8

Nitric oxide ( NO ) is now recognized as a mediator of several biological and immunological functions , but unlike classical neurotransmitters , NO simply diffuse of the postsynaptic cell and around affecting cells. Human chorionic gonadotropin ( hCG ) , produced by placental trophoblasts may act as stimulator on NO synthesis in oocytes of mouse`s ovary. How-ever, in the various organs or cells , the action of hCG on NO synthesis is unknown. We have examined that the effect of hCG on NO synthesis in microglial cells of murine`s brain , using the Griess method. And this study was evident that hCG did not induce NO produc-tion without recombinant interferon gamma ( rIFN-γ) , whereas hCG ( 10∼500 IU/ml ) with rIFN-γ effectively produced NO in microglial cells of brain. As result , NO production in microglial cells increased most significantly in dose of 100 IU/ml of the hCG and the pro-duction of NO was dependent on the dose of hCG ( Table 1 and Fig. 1 ). And NG-monomethyl-L-arginine ( NGMMA ) , competitive inhibitor of NO synthase , reduced the NO production by hCG stimulation with rIFN-γ in microglial cells of murine. Conclusively , this study sugge-sted that hCG stimulate NO production at microglial cells in brain , which may be an important factor for mediating immune and neuroendocrinologic regulation in nervous system.

肺炎桿菌에 長期間 通過시킨 Proteus phage의 物理化學的 因子에 依한 非?化狀況

文永會 全南大醫大 1974 전남의대학술지 Vol.11 No.3

신부전증 환자에서 혈액투석이 GSA와 CK활성도에 미치는 영향에 관한 연구

문영회,조채기,이영진 대한임상병리학회 1988 대한임상병리학회지 Vol.8 No.2

Plastic 포매 조직표본 제작에서 Microwave를 이용한 조직고정에 관한 연구

문형배,정성오,문영회 圓光大學校 1994 論文集 Vol.28 No.2

Microwave를 이용하여 고정한 조직을 plastic 포매하여, 얻은 절편으로 조직화학 염색인 Hematoxylin-Eosin(H-E), periodic acid-Shiff(PAS), reticulum, Masson's trichrome(M·T)염색을 하고 통상적으로 이용되는 포르말린 고정법을 통한 조직표본에서 동일한 염색을 하여 세포의 형태학적 관찰과 염색성을 비교하고자 본 연구를 시도하여 다음과 같은 결론을 얻었다. 1.Microwave조사 시간이 짧고 최종온도가 낮을 수록 조직의 형태학적 소견은 전반적인 세포구조가 파괴되어 불량하고 염생성도 약하였다. 2.Microwave를 이용한 고정에서 최적온도는 장기에 따라 서로 다르며 간장조직은 조사시간 136초, 최종온도59±2℃신장조직은 조사시간 175초, 최종온도 69±2℃에서 세포의 구조가 가장 잘 보존되었고 염색성도 양호하였다. 3.장기별 최적조건에서 PAS, reticulum, Masson's trichrome염색시 결과는 일반적인 포르말린 고정을 한 조직에서 나타난 결과와 일치하였다. 이상의 결과로서 microwave를 이용한 고정방법은 장기별 최적조건이 서로 다르고 고정시간이 짧다는 장점과 다양한 조직화학적 염색에 있어서도 양호한 염색성을 보였으며 전반적으로 형태학적 구조가 잘 보존되었다. This study was undertaken to evaluate the effect of rapid heat fixation on specimens using microwave irradiation. Small portions of the liver and the kidney specimen were fixed by microwave irradiation and subsequently processed by plastic embedding for light microscopical study. The control specimens were fixed by 10% neutral buffered formalin and processed by same method as the experimental ones. Both were stained with hematoxylin and eosin, periodic acid-Schiff, reticulum and Masson's trichrome. The results were as follows: 1.The tissue structures were destroyed and staining property of cells was poor by microwave irradiation at low temperature. 2.The optimal condition of fixation was different in each tissues. For the liver it was 136 seconds microwave irradiation at 59±2℃, for the kidney it was 175 seconds microwave irradiation at 69±2℃. 3. When the specimens were fixed on optimal condition by microwave irradiation, the quality of fixation was as good as that of formalin fixation. Above results suggest that the fixation time can be shortened by microwave fixation.

임신중 내열성인산염분해효소에 관한 연구

심창섭,문영회,백승룡 최신의학사 1975 最新醫學 Vol.18 No.10

Serum alkaline phosphatase is elevated in late pregnancy. The increase of serum alkaline phosphatase in pregnancy is a result of the presence of placental origin enzyme and it shares the heat stability of the placental enzyme. We have examined the levels of heat-stable (placental) serum alkaline phosphatase in subjects studied to establish a base line for studies relating to placental function in this community, and to evaluate it's correlation between heat-stability and EDTA-stability. Serum alkaline phosphatase activities were studied in normal male and female, and in maternal during the pregnancy and the puerperium. There is a negligible activity of the heat-stable alkaline phosphatase in males or in nonpregnant females, but it rises to a significantly elevated level in the late pregnancy and to an even higher level in labor. The level of heat-stable serum alkaline phosphatase in this subjects determined rises from a mean values of 0.3±0.2 King-Armstrong units/100m1. in nonpregnant women to 9.4±5.8 K. A. units/100ml. in women in labor, and it falls slowly after delivery, reaching 52% of the labor level at 5 days postpartum. On comparing the heat and EDTA-stability of the alkaline phosphatase, 48% heat stability and 55.3% EDTA stability of it total activity are observed.

혈액배양으로 분리된 병원성균에 대한 검색

이수경,유재규,문영회 대한임상검사정도관리학회 1990 臨床檢査와 精度管理 Vol.12 No.2

Iodide와 Propylthiouracil 투여로 인한 백서 갑상선의 형태학적 변화

윤성곤,최찬,문영회,문형배 圓光大學校 醫科學硏究所 1989 圓光醫科學 Vol.5 No.1-2

Propylthiouracil and iodide have been widely used as antithyroid agent. But the histologic changes of thyroid gland caused by those drugs are different. The effect of iodide and propylthiouracil to the structure of thyroid were investigated in normal Sprague-Dawley rats. Either Lugol's solution (5%, 1㎖) or propylthiouracil (2.5 ㎎, 1㎖) was treated orally twice a day. The rats were treated for 3 days, 1. 2, 3, 4, and 8 weeks, respectively. In iodide-treated groups, the thyroid revealed inactive follicles until 1 week, but showed normal morphologic features after 2 weeks. In propylthiouracil-treated groups, the follicular epithelial cells became tall columnar cells with few colloid as the duration of treatment increased until 4 weeks. In 8 week-treatment groups, the thyroid structures were similar to those of 4 week-treatment group.