Intracellular IL-4, IL-10, and IFN-gamma levels of leukemic cells and bone marrow T cells in acute leukemia.

Park, Hun Hee,Kim, Myungshin,Lee, Bong-Hee,Lim, Jihyang,Kim, Yonggoo,Lee, Eun Jung,Min, Woo Sung,Kang, Chang Suk,Kim, Won Il,Shim, Sang In,Han, Kyungja Institute for Clinical Science] 2006 Annals of clinical and laboratory science Vol.36 No.1

<P>The quantitative levels of intracellular cytokines IL-4, IL-10, and IFN-gamma (ie, the number of bound PE-conjugated antibody molecules/cell) of leukemic cells and bone marrow T cells (bmT cells) of acute leukemia patients were analyzed by flow cytometry. One hundred, thirty-one (95 AML, 25 ALL, 11 ABL) patients were studied. The leukemic cell IL-4 level was highest in the monocytic AML group (1735 +/- 1056) and lowest in the dysplastic AML group (960 +/- 545). The IFN-gamma level was highest in the acute promyelocytic leukemia (APL) group (495 +/- 159), and lowest in the ALL group (252 +/- 119). The IL-10 level was not significantly different among the diagnosis groups. In bmT cells, the IL-10 level was highest in the dysplastic AML group (972 +/- 1049) and lowest in the APL group (397 +/- 352). The leukemic cell cytokine levels were lowest and bmT cell cytokine levels were highest in the dysplastic AML group. There were no significant correlations of these cytokine levels with 2-yr survival rate, complete remission (CR) rate, or relapse rate. The cytokine levels of bmT cells at the time of CR became normal and were not different among the diagnosis groups. In summary, leukemic cell and bmT cell cytoplasmic expression profiles of IL-4, IL-10, and IFN-gamma are characteristic for each diagnostic group of acute leukemia patients and the profiles of bmT cells are normal at the time of CR.</P>

사람 치은 섬유아세포에서의 Tannerella forsythia 전세균, 막단백질, 당지질에 의한 염증성 사이토카인 발현

김정은,이성훈,최봉규,구기태,김태일,이용무,구영,정종평,류인철,Kim, Jung-Eun,Lee, Sung-Hoon,Choi, Bong-Kyu,Koo, Ki-Tae,Kim, Tae-Il,Lee, Yong-Moo,Ku, Young,Chung, Chong-Pyoung,Rhyu, In-Chul 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.3

Purpose: The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. Material and Methods: After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria. Result: The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-$1{\beta}$ was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs. Conclusion: The membrane protein of T. forsythia could be one of virulence factors.

Bis(p-substituted phenyl) 2-decyloxyterephthalate의 액정 특성에 대한 치환기 효과

박주훈,이종규,최옥병,소봉근,이수민,이준우,진정일,Park, Joo-Hoon,Lee, Jong-Kyu,Choi, Ok-Byung,So, Bong-Keun,Lee, Soo-Min,Lee, Jun-Woo,Jin, Jung-Il 대한화학회 2000 대한화학회지 Vol.44 No.2

Eleven new compounds that are composed of bis(p-substituted phenyl) terephthalate unitand the decyloxy pendant as lateral were synthesized and their thermal and liquid crystalline properties were studied by the differential scanning calorimetry (DSC) and on a hot-stage of a polarizing microscope. The ter-minal substituent groups of the compound were varied; X= -H(II-H), -F(lI-F), -CII(II-CI), -Br(ll-Br), -I(II-I), -$NO_2(lI-NO_2$), $-CF_3(II-CF_3$), -$OC_2H_5(II-OC_2H_5$), -$OC_4H_9(II-OC_4H_9$), -$C_6H_5(Il-C_6H_5$). The compounds of $II-OC_2H_5,\;II-OC_4H_9$ and $II-C_6H_5$ were monotropically nematic. In contrast, the compounds of Il-H, II-F, II-Cl, II-Br, II-I, $lI-NO_2$, $II-CF_3$, and II-CN did not show liquid crystalline properties. 비스(파라-치환페닐)테레프탈산 에스테르의 중앙 테레프탈산 벤젠고리 측면에 데실옥시기가 결합하고 있는 열한개의 새로운 화합물을 합성하였고, 이들의 열적 및 액정 성질을 DSC와 가열판이 부착된 편광현미경을 사용하여 조사하였다. 메소겐의 말단 치환기 X= -H(II-H), -F(II-F), -Cl(Il-Cl), -Br(Il-Br), -I(II-I), -$NO_2(lI-NO_2$), $-CF_3(II-CF_3$), -$OC_2H_5(II-OC_2H_5$), -$OC_4H_9(II-OC_4H_9$), -CN(II-CN) 및 -$C_6H_5(Il-C_6H_5$)로 바꾸었다. $II-OC_2H_5,\;II-OC_4H_9$, 및 $II-C_6H_5$는 단방성 네마틱 액정이었으며, II-H, lI-F, II-Cl, Il-Br, II-I, $II-NO_2$, $II-CF_3$, 및 Il-CN은 액정이 아니었다.

Immunological Roles of Pasteurella multocida Toxin (PMT) Using a PMT Mutant Strain

Kim, Tae-Jung,Toan, Nguyen Tat,Jang, Eun-Jin,Jung, Bock-Gie,Lee, Jae-Il,Lee, Bong-Joo The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.4

The immunological role of the Pasteurella multocida toxin (PMT) in mice was examined using a PMT mutant strain. After a nasal inoculation, the mutant strain failed to induce interstitial pneumonia. Moreover, PMT had no significant effect on the populations of CD4+, CD8+, CD3+, and CD19+ immunocytes in blood or on the populations of CD4+ and CD8+ splenocytes (P<0.01). However, there was a significant increase in the total number of cells in the BAL samples obtained from the wild-type P. multocida-inoculated mice. On the other hand, the level of IL-l expression decreased when the macrophages from the bronchio-alveolar lavage were stimulated with PMT. Overall, PMT appears to play some role (stimulating and/or inhibiting) in the immunological responses but further studies will be required to confirm this.

Anti-photoaging effect of fermented agricultural by-products on ultraviolet B-irradiated hairless mouse skin

CHOI, SUN-IL,JUNG, TAE-DONG,CHO, BONG-YEON,CHOI, SEUNG-HYUN,SIM, WAN-SUP,HAN, XIONGGAO,LEE, SANG JONG,KIM, YOUNG-CHEUL,LEE, OK-HWAN UNKNOWN 2019 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.44 No.2

<P> Processed products from agricultural produce generate a large number of agricultural by-products that contain a number of functional substances. These are often discarded owing to the lack of suitable processing methods. The present study investigated the anti-photoaging properties of fermented rice bran (FRB), soybean cake (FSB) and sesame seed cake (FSC) on ultraviolet B (UVB)-irradiated hairless mouse skin. Results indicated that the oral administration of FRB, FSB and FSC effectively inhibited the UVB irradiation-induced expression of matrix metalloproteinase (MMP)-2, MMP-9, MMP-3 and MMP-13. Reverse transcription-quantitative polymerase chain reaction results also demonstrated that FRB, FSB and FSC significantly inhibited the UVB-induced expression of the genes encoding tumor necrosis factor-α, inducible nitric oxide synthase, interleukin (IL)-6 and IL-1β when compared with the UVB-vehicle group (P<0.05). Additionally, collagen degradation and mast cell infiltration were reduced in hairless mouse skin. Furthermore, UVB-induced wrinkle formation was also significantly reduced in mouse skin compared with the UVB-vehicle group (P<0.05). These results reveal that fermented agricultural by-products may serve as potential functional materials with anti-photoaging activities. </P>

Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro

Oh, Bo Ram,Suh, Dong-hyeon,Bae, Daekwon,Ha, Nina,Choi, Young Il,Yoo, Hyun Jung,Park, Jin Kyun,Lee, Eun Young,Lee, Eun Bong,Song, Yeong Wook BioMed Central 2017 Arthritis research & therapy Vol.19 No.-

<P><B>Background</B></P><P>Histone deacetylase (HDAC) inhibitor has recently been reported to have a therapeutic effect as an anti-inflammatory agent in collagen-induced arthritis (CIA). We investigated the therapeutic effect of a new selective HDAC6 inhibitor, CKD-L, compared to ITF 2357 or Tubastatin A on CIA and regulatory T (Treg) cells in patients with rheumatoid arthritis (RA).</P><P><B>Methods</B></P><P>CIA was induced by bovine type II collagen (CII) in DBA/1 J mice. Mice were treated with HDAC inhibitor for 18 days. Arthritis score was assessed and histological analysis was performed by hematoxylin and eosin (H&E) stain. Cytotoxic T-lymphocyte associated protein (CTLA)-4 expression in induced Treg cells was analyzed and suppression assay was analyzed using Treg cells and effector T (Teff) cells isolated from naive C57BL/6 mice by flow cytometry. Cytokines were analyzed in peripheral blood mononuclear cells (PBMC) of five patients with RA by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). Tumor necrosis factor (TNF) was analyzed using PMA- activated THP-1 cells by ELISA. Suppression assay was analyzed using Treg cells and Teff cells isolated from RA patients by flow cytometry.</P><P><B>Results</B></P><P>In the CIA model, CKD-L and Tubastatin A significantly decreased the arthritis score. CKD-L increased CTLA-4 expression in Foxp3<SUP>+</SUP> T cells and inhibited the proliferation of Teff cells in the suppression assay. In RA PBMC, CKD-L significantly inhibited TNF and interleukin (IL)-1β, and increased IL-10. CKD-L and Tubastatin A inhibited TNF secretion from PMA-activated THP-1 cells. CKD-L and ITF 2357 inhibited the proliferation of Teff cells in RA patients in the suppression assay. Tubastatin A had no effect on inhibition of proliferation.</P><P><B>Conclusion</B></P><P>CKD-L decreased the arthritis score in CIA, reduced the expression of TNF and IL-1β, and increased the expression of IL-10 in PBMC from RA patients. CKD-L increased CTLA-4 expression and the suppressive function of Treg cells. These results suggest that CKD-L may have a beneficial effect in the treatment of RA.</P>

中國과 臺灣의 統一政策比較

정재일,송봉규 尙志大學校 1998 論文集 Vol.20 No.-

This paper is purposed to comparative studying on the unification policy between Taiwan and PRC(People Republic of China). On the unification policy. Taiwan suggests 'Two Regions in One Country', but PRC suggests 'Two Systems in One Country'. What are the meanings of two regions and two systems? What are the differances between 'two regions' and 'two systems'?

교잡종 찰옥수수에 대한 연구 III : Ⅲ. 국내 옥수수 유전자원 수집종에 대한 주요 특성 분포

서정은,주현민,최윤표,홍범용,조양희,주정일,지희정,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

2006년 국내 각 지역으로부터 수집된 재래종옥수수 유전자원을 관행방법에 의해 파종하고 주요특성별로 분포도를 살펴본 결과는 다음과 같다. 1. 수집된 유전자원에 대한 발아율은 대부분 80∼100%이상의 분포를 보였으나, 일부 수집계통에는 30% 미만의 극히 저조한 발아율을 보였다. 2. 수집자원에 대한 초기생육을 살펴보면 극히 생육이 저조한 것이 40% 정도를 보였고, 극히 우수한 자원은 3%미만으로 나타났다. 3. 간장은 대부분이 150∼199cm범위에 55%가 분포하고 있었으나, l00cm이하가 5%, 250cm이상이 8%로 나타나 수집종간에 큰 차이를 보였다. 4. 주당 분얼수는 1개 미만이 40%의 분포를 보였으나, 일부 수집종에서 2 내지 3개의 분얼수를 보이는 계통이 확인되었다. 5. 개화소요일수는 61∼64일의 범위에서 40%, 57∼60일의 범위에서 30%의 분포를 보였으며, 특히 일부 수집종의 경우 55일 이하의 극조생종도 10% 정도의 분포를 보였다. 6. 이삭길이는 대부분 11∼15cm범위에 60%가 포함되어 있으나, 일부계통에서 5cm미만이 2%, 16∼20cm의 이삭길이도 10%가 분포하였다. Germination ratio on the 300 collected genetic resources showed 80 to 100 percentage, but those of some accessions were very low as below 30 percentage. Early growth phase of most of these lines were very poor while some collected lines were very vigor. Range of stem height showed from 150 to 199cm, but that of some accessions showed below 100cm as five percentage and some of them was over 250cm as eight percentage. Number of tillers per plant appeared less one but some collected lines have two to three tillers per plant. In days to tasseling, some accessions showed more four per plant. Ear length of these accession showed above 60 percentage as 11 to 15cm, while that of some collected lines showed over 10 percentage as 16 to 20cm.

벼 立毛中 보리 散播栽培時 적정 立毛數 설정 및 둑새풀 발생의 영향

朱廷一,李喜鳳,申哲雨 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.1

벼 立毛中 보리 산파재배법은 파종노력 절감, 적기파종 및 포장조건 불량시 대체파종 등의 장점이 있지만 관행에 비하여 수량성이 낮고 연차간 變異가 큰 특징이 있다. 수량성을 향상시키기 위하여 栽培安定性을 높이는 관리기술이 요구되는데 우선 입모 확보와 둑새풀 방제체제 확립이 절실히 요구된다. 따라서 立毛率 향상시킬수 있는 요인, 다수확을 위한 적정 입모수 설정 및 둑새풀에 의한 감수정도 등을 구명코자 대전에서 올보리를 공시하여 벼 수확 1일전에 파종하고 이에 대한 결과는 다음과 같다 . 1. 볏짚피복과 진압에 의하여 출현율이 향상되었는데 볏짚피복효과가 진압효과에 비하여 높았고 추가진압으로 11% 증수 효과를 기대할 수 있었다. 2. 단위 면적당 적정보리 立毛數는 507개/㎡로 측정되었다. 3. 둑새풀 발생이 많을수록 수량이 감소되었는데 ㎡당 17~22본 발생시 21.8%, 6~11본 발생시 11.0%가 각각 감수되었다. This experiment was carried out to estimate the appropriate seedling stand and to find out the factors of good establishment and the reason of the reduction of yield by water foxtail. The seedling stands was improved by the ice straw mulching and treading by the tracks of combine. The effect of mulching was higher than the treading. The appropriate seedling stands for example yield were estimated about 507 plants per ㎡ and its yield was 24㎏/10a. The higher density of water foxtail, the less the grain yield; 21.8% by 17 to 22 water foxtail stands per ㎡ and 11.0% by 6 to 11 plants.

기니픽에서 Kanamycin 이독성

박찬일,송영복,정봉진,박용호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To evaluate experimental kanamycin - induced ototoxicity, changes of hearing loss were observed after administration of kanamycin in guinea pig, using auditory brainstem rcsponse(ABR) audiometry. Kanamycin(KM), 50mg/kg/day(group I), 100mg/kg/day(group Ⅱ), 200mg/kg/day(group Ⅲ) and 400mg/kg/day(group IV), was injected intramuscularly in each group and ABR thresholds were measured every other days through 50 days after the initial administration of KM. The injection of KM was discontinued at the day when the ABR threshold was increased more than 40 dB in each guinea pig. After the discontinuation of KM injection, ABR thresholds recovered progrssively to the base line level before the day 50 in group I and Il. But the ABR threshold changes were progressed after discontinuation of KM injection and finally they showed no responses in group III and IV. The results suggested that high dose KM administration could result progressive and irreversible hearing loss after the discontinuation of KM administration.