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한국인 Actinobacillus actinomycetemcomitans 균주의 특이 독성 clone형과 혈청형 및 백혈구독성과의 관계
구영,Ku, Young 대한치주과학회 1995 Journal of Periodontal & Implant Science Vol.25 No.3
Previous studies have demonstrated that not all A. actinomycetemcomitans produced significant level of leukotoxic factor and its leukotoxicity have associated with serotype and genetic variation. Our aim was to investigate on the interrelationship between serotype and leukotoxicity of an A. actinomycetemcomitans consisting of 13 clinically well characterized. Korean isolates and to evaluate if particular virulent clonal types of A. actinomycetemcomitans are associated with periodontal disease. For this study, 13 strains of A. actinomycetemcomitans from 6 patients with periodontal disease were isolated and identified by using a selective medium(tryptic soy agar supplemented with 10% serum, $75{\mu}g$ of bacitracin and $5{\mu}g$ of vancomycin per ml) in 10% C02 incubator for 3days with routine Gram staining, colony morphology and biochemical test..For serotyping, antisera were prepared from reference strains of 5 serotypes. (ATCC 29523,Y4, SUNY aB 67, IDH 781, IDH 1705) and then ammonium sulfate precipitation, immunoabsorption and indirect immunofluoroscent procedures were done. For analysis of leukotoxicity, sonic extract of A. actinomycetemcomitans exposed to PMN, and trypan blue was stained for counting the cell viability. Finally Southern blot analyses of genomic DNA digested with the restriction enzyme Tag I was done and the Southern blots were hybridized with the 530bp fragment, termed delta 530, originating from the ltx promoter of strain 652 and deleted from strain JP2. Also ltxA-3.1 and SC2 probe from strain JP2 were hybridized with genomic DNA fragments. Results reveal that strains isolated showed approximately equal proportions of 3 serotypes(b, d, e) and serotype b was not detected. 2 patients harbored 2 different serotypes in the same disease site. The prevalence of leukotoxic strain was 23% and there was no relationship between serotype, leukotoxicity and clinical observations. Especially virulent clonal types of Actinobacillus actinomycetemcomitan (JP2 strain) could not found. Further studies are necessary on the genetic polymorphism of leukotoxin and its relations to clinical status.
혈소판유래 성장인자 함유 흡수성 차폐막이 치주조직의 재생에 미치는 영향
구영,김정은,한수부,정종평,박윤정,이승진,권영혁,Ku, Young,Kim, Jeong-Eun,Han, Soo-Boo,Chung, Chong-Pyoung,Park, Yoon-Jeong,Lee, Seung-Jin,Kwon, Youg-Hyuk 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.1
PDGF-BB has been recognized as a highly potential growth factor for guided tissue regeneration in periodontal defect. This study carried out histologic and histometric evaluation of $200ng/cm^2$ PDGF-BB loaded bioresorbable membrane made from polyglycolic and polylactic acid. It was tested for its biocompatibility, ability to prevent epithelial downgrowth and amount of periodontal regeneration. Without membrane and PDGF-BB unloaded bioresorbable membrane were used as control. Healthy six beagle dogs were used. Each dog was anesthetized and buccal flaps were reflected in the mandibular and maxillary premolar areas. Buccal alveolar bone between the mesiobuccal and distobuccal line angles was surgically removed on the lower 2nd and 4th premolar in mandible, 2nd premolar in maxilla, to a level 4mm apical to the cementoenamel junction with creating a Class II buccal furcation defect for available space. Care was taken not to remove the root cementum layer and rubber impression materials were placed over each surgically created defect. Flaps were repositioned and sutured. Reconstructive surgery was performed 1 month after defect preparation. PDGF-BB loaded membranes and controls were randomly placed on maxillary 2nd premolars and mandibular 2nd and 4th premolars. Plaque control regimen was instituted with daily brushing with a 0.1% chlorhexidine digluconate during experimental periods. The animals were sacrificed 2 and 5 weeks after surgery and undecalcified specimens were prepared for histologic evaluation. The degree of coronal regrowth of new bone, new cementum and the amonut of new bone areas formed on the defected area of the PDGF-BB loaded membrnae turned superior to without membrane and drug unloaded membrane. Experimental membrane could prevent the epithelial downgrowth irrespective of drug loaded or not and showed good biocompatiblity, These results implicated that PDGF-BB loaded bioresorbable membrane could be highly useful tool for guided tissue regeneration of periodontal defects.
이온 빔 조사 처리된 키토산 스펀지의 세포적합도에 관한 연구
구영,Ku, Young 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.2
Chitosan is a biodegradable and non-toxic material with a molecular weight of 800-1,500Kd which can be obtained in various forms with extraordinary chemical structures and biological characteristics of which enables it to be used in many fields as a biomaterial. Ion irradiation is a useful tool to modify chemical structures and physical properties of high molecular weight polymers. The basic hypothesis of this study is that when surface properties of chitosan in a sponge form are modified with ion beam-irradiation and cell adhesion properties of chitosan would improve and thereby increase the regenerative ability of the damaged bone. The purpose of this study was to illuminate the changes in the cytocompatibility of chitosan sponges after ion beam-irradiation as a preliminary research. Argon($Ar^+$) ions were irradiated at doses of $5{\times}10^{13}$, $5{\times}10^{15}$ at 35 keV on surfaces of each sponges. Cell adhesion and activity of alkaline phosphatases were studied using rat fetal osteoblasts. The results of this study show hat ion beam-irradiation at optimal doses($5{\times}10^^{13}\;Ar^+\;ion/cm^2$) is a useful method to improve cytocompatibility without sacrificing cell viability and any changing cell phenotypes. These results show that ion beam-irradiated chitosan sponges can be further applied as carriers in tissue engineering and as bone filling materials.
조산 또는 만삭 분만 후 진단된 뇌성마비의 임상적 양상
구영아 ( Young Ah Koo ),최동석 ( Dong Seok Choi ),최석주 ( Suk Joo Choi ),오수영 ( Soo Young Oh ),장윤실 ( Yun Sil Chang ),박원순 ( Won Soon Park ),김현숙 ( Hyeon Sook Kim ),노정래 ( Cheong Rae Roh ),김종화 ( Jong Hwa Kim ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12
목적: 조산 또는 만삭에 출생한 이후 뇌성마비 진단을 받은 환아의 주산기 병력과 뇌성마비의 임상 양상을 비교하고자 하였다. 연구 방법: 1994년 10월부터 2004년 12월까지 본원에서 출생 후 뇌성마비 진단을 받은 총 75명의 환아의 의무기록을 후향적으로 분석하였다. 주요 기형이 동반된 다섯 명을 제외한 총 70명을 대상으로 조산군과 만삭군으로 분류하여 각 군의 주산기 병력과 뇌성마비의 형태, 침범 부위 및 중증도를 비교하였다. 결과: 뇌성마비의 발생 빈도는 0.23%이었고, 출생 시 재태 연령이 증가할수록 감소하였다. 다태 임신에서의 뇌성마비 발생 빈도는 단태 임신보다 6.7배 높았고, 남아가 여아보다 1.5배 많았다. 조산군은 55명 (79%), 만삭군은 15명 (21%)이 포함되었다. 조산군에서 유의한 신생아 합병증이 동반된 경우는 86%였고, 만삭군에서는 15명 중 6명에서 저산소성 허혈성 뇌손상, 태변흡입증후군, 또는 원인 불명의 경련을 보였다. 조산군의 뇌성마비 형태의 대부분은 경직형 (95%)임에 반해 만삭군에서는 경직형이 67%, 무정위 운동형이 20%, 근긴장 이상형이 7%, 저긴장형이 7%로 다양하게 나타났다. 뇌성마비의 침범 부위는 조산군에서 하지 마비가 가장 많았고, 만삭군에서는 사지마비가 가장 많았다. 결론: 만삭아의 뇌성마비는 조산아에 비해 신생아 합병증 동반이 유의하게 적은 반면 뇌성마비의 형태와 침범부위는 다양하게 나타났다. 이는 만삭아의 뇌성마비가 보다 다양한 원인과 임상 양상을 가짐을 시사한다. Objective: To investigate the perinatal and clinical characteristics of cerebral palsy (CP) following preterm or term birth. Methods: A total of 75 infants born and diagnosed as CP in our hospital from October 1994 to December 2004 were recruited retrospectively. Their maternal and perinatal outcomes and the type, involved lesion and severity of CP were analyzed. Results: The incidence of CP was 0.23%, which showed decreasing pattern according to advancing gestational age at birth. CP was more frequent (6.7-times) in multifetal pregnancy. Male to female ratio was 1.5: 1. After excluding five infants with major congenital anomalies, 55 (79%) infants were born before 37 weeks` gestation (preterm CP) and 15 (21%) infants were born beyond 37 weeks` gestation (term CP). Eighty-six percent of preterm CP had significant neonatal morbidities, but only 6 out of 15 infants in term CP had significant perinatal events including hypoxic ischemic encephalopathy, meconium aspiration syndrome, and seizure of unknown origin. The most common type of preterm CP was spastic (95%), whereas the types of term CP were more diverse; spastic in 67%, athetoid in 20%, dystonic in 7%, and hypotonic in 7%. Regarding the involved lesions, the most common type was diplegic in preterm CP and quadriplegic in term CP. Conclusion: In contrast to preterm CP, term CP had significantly less perinatal risk factors, and their type and involved lesion showed more diverse patterns. These findings may implicate that more heterogenous etiologies are involved in pathogenesis of term CP.