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( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1
The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.
고등학교 교사의 전문적 자본이 교직만족도에 미치는 영향 : 학교장 지도성과 학교풍토의 직렬다중매개효과 분석을 중심으로
한유경,설가인,이은지,김하영 이화여자대학교 사범대학 교과교육연구소 2022 교과교육학연구 Vol.26 No.6
This study examined the structural relationship between professional capital and teacher satisfaction of high school teachers. For the analysis, data from 3,614 high school teachers who responded to the 9th year of the GEPS were used, and the serial multiple mediation effect of principal leadership and school climate was analyzed in the relationship between professional capital and teacher satisfaction. The result of the analysis showed that, human capital, a sub-element of professional capital, had a positive effect on principal leadership and teacher satisfaction, and social capital had a positive effect on the school climate. Decision capital had a positive effect on all of principal leadership, school climate, and teacher satisfaction. Principal leadership significantly increased the vitality of the school climate, and principal leadership and school climate had a positive effect on teacher satisfaction. In mediation effect, principal leadership showed significant mediation effect between mediated human capital and teacher satisfaction, and between decision capital and teacher satisfaction. The school climate had a significant indirect effect on the relationship between social capital and teacher satisfaction, decision capital and teacher satisfaction. In addition, the effect mediated by school principal leadership through the school climate was significant in the relationship between human capital and teacher satisfaction, decision capital and teacher satisfaction. Based on the above results, implications for improving teacher satisfaction were drawn. 이 연구는 고등학교 교사의 전문적 자본과 교직만족도 간의 구조적 관계를 검증하고자 하였다. 이를 위해 경기종단연구 9차년도 조사에 응답한 고등학교 교사 중 3,614명을 대상으로 전문적 자본과 교직만족도의 관계에서 학교장 지도성과 학교풍토의 직렬다중매개효과를 분석하였다. 분석 결과, 전문적 자본의 하위요소인 인적 자본은 학교장 지도성과 교직만족도에, 사회적 자본은 학교풍토에 긍정적인 영향을 미쳤다. 의사결정적 자본은 학교장 지도성, 학교풍토, 교직만족도 모두에 정적인 효과를 지니는 것으로 나타났다. 학교장 지도성은 학교풍토의 활력을 유의하게 높였고, 학교장 지도성과 학교풍토는 교직만족도에 긍정적인 영향을 주었다. 매개효과를 검증한 결과, 학교장 지도성은 인적 자본과 교직만족도, 의사결정적 자본과 교직만족도를 유의하게 매개하였다. 학교풍토는 사회적 자본과 교직만족도, 의사결정적 자본과 교직만족도 간의 관계에 있어 간접효과가 유의하였다. 또한, 학교장 지도성이 학교풍토를 거쳐 매개한 효과는 인적 자본과 교직만족도, 의사결정적 자본과 교직만족도 간의 관계에서 유의하였다. 이상의 결과를 토대로 교직만족도 제고를 위한 시사점을 도출하였다.
OB-25 : Development of a prediction model of probability of successful vaginal delivery
( Ji Yeon You ),( Hye Ran Lee ),( Sook Young Woo ),( So Yeon Yoon ),( Ye Ri Lee ),( Jung Joo An ),( Suk Joo Choi ),( Cheong Rae Roh ),( Jong Hwa Kim ),( Soo Young Oh ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-
Prediction of successful vaginal delivery in laboring women is clinically assessed by obstetricians depending on various factors associated with labor progression and maternal demographic characteristics. In this study, we tried to generate a prediction model based on these factors that can provide the arithmetical chance of successful vaginal delivery in real time for laboring women. We collected the clinical information from 1661 consecutive singleton pregnancies delivered at term from January 2008 to June 2009. The clinical information including maternal age, body mass index (BMI), gestational age at admission, parity, presence of labor induction, hourly cervical dilatation, time at examination, mode of delivery, and staff in charge was reviewed. Logistic regression analysis and generalized estimating equation were used to generate a prediction model. By the logistic regression analysis, we found that maternal age, BMI, gestational age at admission, parity, presence of induction, and hourly cervical dilatation were significant variables associated with successful vaginal delivery (p<0.05), but staff in charge were not (p=0.342). Using these independent variables, a prediction model that can provide the arithmetical chance of successful vaginal delivery in real time was generated. Areas under the receiver-operating characteristic curves of this prediction model was 0.94. Our prediction model could be used to provide the arithmetical chance of successful vaginal delivery in real time for laboring women.