D-Amphetamine이 니코틴성 흥분작용에 의한 카테콜아민 분비작용에 미치는 영향

임건한,서유석,민선영,임지연,김용직,나광문,임동윤 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

본 연구의 목적은 d-arphetamine이 흰쥐의 적출부신 관류모델에서 니코틴 수용체 흥분에 의한 카테콜아민(CA) 유리작용에 미치는 영향을 검색하여 그 작용기전을 규명하고자 하였다. D-amphetamine은 흰쥐 부신정맥내로 60분간 관류시 d-amphetamine자체는 약한 CA 분비작용을 나타내었으나, d-amphetamine (30 μM)을 비롯한 강력한 neuronal nicotine 수용체 작용제인 cytisine (50 μM) 및 epibatidine (30 μM)에 의한 CA 유리작용을 처음 4-10분 동안만 유의하게 증강시켰다. 또한, d-amphetamine (30 μM)은 60분간 부신정맥 내로 관류한 상태에서 dihydropyridine L-형 칼슘통로 개방약물인 Bay-K-8644 (10 μM)과 세포질내 칼슘저장고에서 Ca^(2+) ATPase 억제제인 cyclopiazonic acid (10 μM)의 CA 유리작용을 처음 4분간만 유의하게 증강시켰다. 그러나, 고농도의 d-amphetamine (500 μM)은 상기한 모든 분비촉진제의 CA분비작용을 오히려 억제하였다. 이와 같은 연구결과로 보면, 흰쥐 관류 부신수질에서 d-amphetamine은 낮은 농도에서는 콜린성 니코틴 수용체 흥분에 의한 카테콜아민 분비반응을 증강시키지만, 고농도에서는 오히려 억제적으로 작용함을 시사한다. 따라서, d-amphetamine은 용량에 따라서 흰쥐 적출 관류부신수질의 니코틴 수용체의 작용제 및 길항제로 이중 작용(dual action)을 나타내는 것으로 생각된다. 이러한 d-amphetamine의 작용은 흰쥐 부신수질 크롬친화세포의 dihydropyridine계 L-형 칼슘통로의 활성화 및 세포 내 칼슘저장고로부터 칼슘유리작용과 관련성이 있는 것으로 사료된다. The purpose of the present study was to examine the effect of d-amphetamine on CA release evoked by nocotinic receptor stimulation from the isolated perfused model of the rat adrenal gland, and to establish its mechanism of action. D-amphetamine(30 μM), when perfused into an adrenal vein of the rat adrenal gland for 60 min, enhanced the CA secretory responses evoked by ACh (5.32 mM), nicotine (30 μM), cytisine (50 μM, a selective neuronal nicotinic Nn-receptor agonist) and epibatidine (30 nM, a selective neuronal nicotinic Nn receptor agonist) only for the first period (4~10 min), although it alone has weak effect on CA secretion. Moreover, d-amphetamine (30 μM) in to an adrenal vein for 60 min also augmented the CA release evoked by BAY-K-8644, an activator of the dihydropyridine L-type Ca^(2+) channels, and cyclopiazonic acid, an inhibitor of cytoplasmic Ca^(2+) ATPase only for the first peroid (4 min). However, in the presence rather inhibited the CA secretory responses evoked by the above all of secretagogues. Taken together, these experimental results suggest that d-amphetamine at a low concentration enhances the CA secretion from the rat adrenal medulla evoked by stimulation of cholinergic nicotininc receptors, but at a high concentration it rather inhibits them. It semms that d-amphetamine has dual action acting as both agonist and antagonist at nicotinic receptors of the isolated perfused rat adrenal medulla, which are might be dependent on the concentration. It is also thought that these actions of d-amphetamine are probably relevant to the activation of the dihydropyridine L-type Ca^(2+) channels located on the rat adrenomedullary chromaffin cell membrane and release of Ca^(2+) from the cytoplasmic store.

Poster Session:PS 1172 ; Cardiology : Determinants of Microvascular Dysfunction in St-Segment Elevation Myocardial Infarction

( Gwang Seok Yoon ),( Sang Don Park ),( Yong Soo Baek ),( Man Jong Lee ),( Sung Hee Shin ),( Dae Hyeok Kim ),( Jun Kwan ),( Keum Soo Park ),( Seong Ill Woo ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: We sought to fi nd differences of clinical and angiographic characteristics in ST-segment elevation myocardial infarction(STEMI) patients with or without coronary microvascular dysfunction by index of microcirculatory resistance (IMR). Methods: STEMI patients who underwent primary percutaneous coronary intervention (PCI) were enrolled. Baseline characteristics including clinical and angiographic characteristic were investigated in all patients. The IMR, parameter of hyperemic microvascular resistance, was measured with a pressure sensor/thermistor-tipped guide-wire after primary percutaneous coronary intervention (PCI) Results: 113 STEMI patients (age=56±11 years, M:F=95:18) were enrolled and 113 culprit lesions of coronary artery were analyzed. The patients were divided into three groups based on following the value of IMR: Low-IMR (n=38, IMR=12. 9±2. 6 U), Mid- IMR (n=38; IMR=23. 9±4. 0 U) and High-IMR group (n=37; IMR=48. 1±17. 1 U). Mean age of Low-IMR was signifi cantly younger than Mid-IMR and High-IMR. Mean doorto- balloon times were under 90 minutes in all IMR groups, and there were no significant differences among each IMR groups. However, symptom-onset-to-balloon time was signifi cantly longer in High-IMR than Mid-IMR and Low-IMR (p<0. 001). The high IMR group included the more frequent proximal location of culprit lesion than non-proximal location (p=0. 008). In multivariate regression analysis, age and symptom- onset-to-balloon time were independent determinants of higher IMR (p=0. 013 for age, p=0. 003 for symptom-onset-to-balloon time). Conclusions: Our data suggests that in STEMI patients with mean door-to-balloon time under 90minutes, age and symptom-onset-to-balloon time may be the main determinants of impaired microcirculatory resistance.

S-517 : Streptococcus intermedius liver abscess with septic endophthalmitis and septic pulmonary emboli

( Gwang Seok Yoon ),( Jae Hyoung Im ),( Ji Hyeon Baek ),( Jin Soo Lee ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

간 농양에서 균혈증에 의해 발생한 전이감염은 종종 증례 보고가 있었고 주된 세균은 Klebsiella pneumoniae로 알려져 있다. 본 저자들은 Streptococcus intermedius에 의한 간 농양에서 패혈성 안구내염과 패혈성 폐색전증이 동반된 증례를 보고하는 바이다. 40세 남자가 4일 전부터 시작된 근육통과 우측 눈의 이물감으로 내원하였다. 과거력과 가족력은 특이 소견 없었다. 신체 진찰에서 38.7℃의 발열이 있었고 우측 결막은 충혈되어 있었다. 흉부 청진에서 우하폐야에서 수포음이 들렸고 복부 진찰에서 압통과 반동압통은 없었다. 혈액 검사에서 백혈구 23,630/μL, 혈색소 15.3 g/dL 그리고 혈소판은 471,000/μL이었다. C-reactive protein (CRP) 35.3 mg/dL, 적혈구침강속도(ESR) 94 mm/hr로 상승 되어 있었다. 단순 흉부 방사선 x-ray 에서 우중폐야에서 혼탁이 보였다. 안저 검사 및 세극등 검사에서는 안구내염 소견이 보였다. 원발성 폐렴과 동반된 패혈성 안구내염 가능성이 가장 높을 것으로 판단하여 경험적 항생제(cefotaxime, azithromycin)를 투여하였다. 하지만 단순 흉부 방사선 x-ray에서 폐렴양 병변이 증가하였고 임상 양상은 호전되지 않았다. 혈액배양검사 결과 Viridans group Streptococci가 자랐고, 최종 동정 결과 Streptococcus intermedius가 확인되었다. 원발 병소를 파악하기 위해 흉부 컴퓨터 단층촬영을 시행하였고 패혈성 폐색전증으로 보이는 우폐하엽의 흉막하 경화와 간 우엽의 농양이 보였다. 균혈증의 다른 원발 병소 확인을 위해 경흉부 심초음파 검사와 대장내시경 검사를 하였고 특이 소견은 발견되지 않았다. 초음파 유도하 경피적 농양 세침 흡인 배양 검사에서 Streptococcus intermedius가 자라 원발성 간 농양의 균혈증에 의한 패혈성 안구내염과 패혈성 폐색전증으로 진단하였다. 환자는 세균 배양 감수성 결과에 따라 cefotaxime을 사용하였고 간 농양에서의 혐기성 미생물 동반 감염 가능성을 염두에 두고 metronidazole을 함께 사용하였다. 간 농양에 대해 초음파 유도하 경피적 농양 배액술을 시행하였다. 안구내염에 대해서는 유리체내 약물 주사법을 2차례 시행하였으나 호전 보이지 않아 유리체절제술을 2차례 시행 하였다. 환자는 시력의 큰 호전은 없었으나 발열과 혈액 검사 결과가 크게 호전되어 입원 29일째 경구 항생제를 유지한 채 퇴원하였고, 퇴원 35일 째 항생제 치료를 중단한 후 외래에서 추적관찰 중이다. Streptococcus intermedius에 의한 균혈증은 흔하지 않지만 수반되는 감염이 있을 경우에는 기저 농양을 항상 염두에 두여야 할 것으로 생각된다.

Neutrophil-to-Lymphocyte Ratio at Emergency Room Predicts Mechanical Complications of ST-segment Elevation Myocardial Infarction

Yoon Gwang-Seok,Choi Seong Huan,Woo Seong-Ill,Baek Yong-Soo,Park Sang-Don,Shin Sung-Hee,Kim Dae-Hyeok,Kwan Jun,Lee Man-Jong,Kwon Sung Woo 대한의학회 2021 Journal of Korean medical science Vol.36 No.19

Background: The neutrophil-to-lymphocyte ratio (NLR) has been proven to be a reliable inflammatory marker. A recent study reported that elevated NLR is associated with adverse cardiovascular events in patients with ST-segment elevation myocardial infarction (STEMI). We investigated whether NLR at emergency room (ER) is associated with mechanical complications of STEMI undergoing primary percutaneous coronary intervention (PCI). Methods: A total of 744 patients with STEMI who underwent successful primary PCI from 2009 to 2018 were enrolled in this study. Total and differential leukocyte counts were measured at ER. The NLR was calculated as the ratio of neutrophil count to lymphocyte count. Patients were divided into tertiles according to NLR. Mechanical complications of STEMI were defined by STEMI combined with sudden cardiac arrest, stent thrombosis, pericardial effusion, post myocardial infarction (MI) pericarditis, and post MI ventricular septal rupture, free-wall rupture, left ventricular thrombus, and acute mitral regurgitation during hospitalization. Results: Patients in the high NLR group (> 4.90) had higher risk of mechanical complications of STEMI (P = 0.001) compared with those in the low and intermediate groups (13% vs. 13% vs. 23%). On multivariable analysis, NLR remained an independent predictor for mechanical complications of STEMI (RR = 1.947, 95% CI = 1.136–3.339, P = 0.015) along with symptom-to balloon time (P = 0.002) and left ventricular dysfunction (P < 0.001). Conclusion: NLR at ER is an independent predictor of mechanical complications of STEMI undergoing primary PCI. STEMI patients with high NLR are at increased risk for complications during hospitalization, therefore, needs more intensive treatment after PCI.

신장 침범이 동반된 고령의 성인형 스틸병 치험

윤광석 ( Gwang Seok Yoon ),박원 ( Won Park ),백지현 ( Ji Hyeon Baek ),한지영 ( Jee Young Han ),정경희 ( Kyong Hee Jung ),임미진 ( Mei Jin Lim ),주고운 ( Ko Woon Joo ),권성렬 ( Seong Ryul Kwon ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.6

Adult-onset Still`s disease (AOSD) is a rare systemic inflammatory disease characterized by spiking fever, arthralgia, salmon pink rash, neutrophilic leukocytosis, and multi-organ involvement. Although renal involvement may appear in some cases of adult Still`s disease, onset over 70 years of age with renal involvement has not been described. We report a 73-years-old woman whose illness manifested with fever of unknown origin, massive proteinuria, and multiple lymph nodes enlargement. With proteinuria of 2,650 mg/day, a renal biopsy was performed, and histopathological evaluation yielded the diagnosis of chronic glomerulonephritis (CGN). After excluding infectious disease, malignancy, and other rheumatic disease, AOSD was diagnosed with symptoms including fever over 39.0oC for more than a week, leukocytosis, generalized lymphadenopathy, and negative autoantibodies. Proteinuria and fever were improved markedly by high dose glucocorticoids and methotrexate therapy.

이종 집적을 위한 비전도성필름 소재 기술

최광문(Gwang-Mun Choi),주지호(Jiho Joo),윤호경(Ho-Gyeong Yun),문석환(Seok Hwan Moon),장기석(Ki-Seok Jang),이찬미(Chanmi Lee),오진혁(Jin-Hyuk Oh),계인석(In-Seok Kye),문윤환(Yoon-Hwan Moon),최광성(Kwang-Seong Choi),엄용성(Yong-Sung Eom 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.6

An Analysis of the Safety Education for Foreign Laborer in Construction Site

Shin, Yoon Seok,Nam, Seok Hoon,Kim, Tae Yong,Kim, Gwang Hee Trans Tech Publications 2013 Advanced materials research Vol.671 No.-

<P>In recent years, the number of foreign workers employed on construction sites in Korea has been on the rise, and industrial accidents involving foreign workers have become a social issue. Yet despite this fact, the interest in safety education for foreign workers has still been relatively low. For this reason, the purpose of this study is to analyze the current state of safety education for foreign construction workers. To conduct an analysis of the current status, we surveyed foreign construction workers currently working at construction sites. Based on the results of this survey, we have derived an outline for an improvement plan founded on an awareness of and problems in safety education for foreign workers.</P>

국제 소식 - (ASCE, JSCE)

윤광석(Gwang-seok Yoon),김종오(Jong-o Kim) 대한토목학회 2014 대한토목학회지 Vol.62 No.6

PGE<SUB>2</SUB> Regulates Pacemaker Currents through EP<SUB>2</SUB>-Receptor in Cultured Interstitial Cells of Cajal from Murine Small Intestine

Seok Choi,Kyung Won Cho,Jong-Hyun Reu,Jun-Soo Kim,Hyun Sik Mun,Myung Young Kim,Kwang Chul Park,Gwang Sik Heo,Sung Jong Chang,Cheol Ho Yeum,Pyung Jin Yoon,Jae Yeoul Jun 대한생리학회-대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.13 No.1

The interstitial cells of Cajal (ICCs) are the pacemaker cells in gastrointestinal tract and generate electrical rhythmicity in gastrointestinal muscles. Therefore, ICC may be modulated by endogenous agents such as neurotransmitter, hormones, and prostaglandins (PGs). In the present study, we investigated the effects of prostaglandins, especially PGE<SUB>2</SUB>, on pacemaker currents in cultured ICCs from murine small intestine by using whole-cell patch clamp techniques. ICCs generated spontaneous slow waves under voltage-clamp conditions and showed a mean amplitude of ⁣452⁑39 pA and frequency of 18⁑2 cycles/min (n=6). Treatments of the cells with PGE<SUB>2</SUB> (1μM) decreased both the frequency and amplitude of the pacemaker currents and increased the resting currents in the outward direction. PGE<SUB>2</SUB> had only inhibitory effects on pacemaker currents and this inhibitory effect was dose-dependent. For characterization of specific membrane EP receptor subtypes, involved in the effects of PGE<SUB>2</SUB> on pacemaker currents in ICCs, EP receptor agonists were used: Butaprost (1μM), EP<SUB>2</SUB> receptor agonist, reduced the spontaneous inward current frequency and amplitude in cultured ICCs (n=5). However sulprostone (1μM), a mixed EP<SUB>1</SUB> and EP<SUB>3</SUB> agonist, had no effects on the frequency, amplitude and resting currents of pacemaker currents (n=5). SQ-22536 (an inhibitor of adenylate cyclase; 100μM) and ODQ (an inhibitor of guanylate cyclase; 100μM) had no effects on PGE<SUB>2</SUB> actions of pacemaker currents. These observations indicate that PGE<SUB>2</SUB> alter directly the pacemaker currents in ICCs, and that the PGE<SUB>2</SUB> receptor subtypes involved are the EP<SUB>2</SUB> receptor, independent of cyclic AMP- and GMP-dependent pathway.

공간 점유 병소로서 간경변과 동반하여 발생한 무산소성 가성분엽성 간 괴사 1예

백광호,고문수,한상진,정의훈,김명빈,윤동석,장웅기,김동준 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.31 No.3