키토산 가공이 모직물의 특성에 미치는 효과

유혜자,이혜자,모태화 한국의류학회 2000 한국의류학회지 Vol.24 No.1

The changes on shrinkage, dyeability, antimicrobial activities and other physical properties of wool fabrics by chitosan treatment were investigated. A acid dye, a basic dye and natural artemisia were used for dyeing of wool fabrics. For Antimicrobial test of wool fabrics, S.Aureus was used. As the results, The wool fabrics treated with chitosan showed a marked decrease in shrinkage. Dyeability increased slightly as concentration of chitosan treatment increased. Dyeability of acid dye was the highest than other dyes. On the other hand, the dyeability of basic dye on chitosan treated fabrics was lower than untreated fabrics. Reduction rates of colony of chitosan-treated wool fabrics showed from 64.8% to 85.24% as chitosan concentration increased. Reduction rates of colony of the chitosantreated fabrics decreased by dyeing with acid and basic dye. But increased by retreating with chitosan. Fabrics dyeing with artemisia showed above 90% reduction rate against S.Aureus on all chitosan concentrations. The Tensile properties of chitosan-treated fabrics showed little decrease. The moisture regain and the air permeability was decreased on chitosan concentration on 0.1% but recovered as chitosan concentration increased. The warmth retention was increased a little by chitosan treatment. The drapeability became hard as chitosan concentration increased.

음이온성기온 carboxy 기를 가진 chitosan 유도체의 제조와 그들의 응집 특성에 관한 연구

이배훈 ( Bea Hoon Lee ),정병옥 ( Byung Ok Jung ),손태일 ( Tae Il Son ),김천호 ( Chun Ho Kim ) 한국키틴키토산학회 2003 한국키틴키토산학회지 Vol.8 No.1

양이온성 천연 고분자 전해질인 chitosan에 음이온기의 도입에 따른 특징을 연구하기 위해 chitosan을 4-chlorobutyric acid 또는 5-chlorovaleric acid와 반응시킴으로써 n-propyl, n-butyl기를 가지는 새로운 carboxyalkyl chitosan 유도체를 합성하였다. 합성된 carboxyalkyl chitosan 유도체들의 구조적인 변화는 EA와 FT-IR로 확인하였다. 이들 carboxyalkyl chitosan 유도체들은 chitosan에 비해 낮은 결정성을 가짐을 X-선 분석을 통해 확인할 수 있었다. 또한 카올린 현탁액을 이용한 응집 실험 결과는 pH가 5,carboxyalkyl chitosan의 농도가 5ppm, 25ppm 일 때 최적의 응집 성능을 나타내었다. Novel carboxyalkyl chitosan derivatives with n-propyl, n-butyl groups were prepared by reacting chitosan with 4-chlorobutyric acid and 5-chlorovaleric acid. The structural changes in the samples were confirmed by using EA and FT-IR. Resulting chitosan derivatives have the low crystallinity, which were confirmed by XRD. Their flocculating behavior, in kaloine suspension showed the optimum property at pH 5 and 5 ppm, 25 ppm concentration of resin conditions.

고추 친환경 재배를 위한 Chitosan 처리 효과

문영훈(Young-Hun Moon),이진호(Jin-Ho Lee),안병구(Byung-Koo Ahn),최인영(In-Young Choi),정성수(Seong-Soo Cheong) 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.4

Chitosan이 친환경 고추재배를 위한 친환경자재로써 토양환경개선과 고추수량 등에 미치는 효과를 조사하기 위해 엽면시비, 토양관주, 두 가지를 혼합처리 하였다. 침지시간에 따른 발아율은 무처리에서 99.4%였고, chitosan 농도가 낮을수록 침지시간이 짧을수록 발아율은 높았지만 대조구보다는 낮았다. 수확기에 조사한 토양화학성분은 chitosan 처리방법에 관계없이 정식전 보다 모두 감소하였다. Chitosan 처리횟수가 증가할수록 토양중 세균과 방선균 밀도는 증가하였고, chlorophyll 함량은 대조구보다 많았다. 고추 잎의 P 함량은 차이가 없었지만, N는 14일 간격으로 엽면시비를 하거나 21일 간격으로 토양관주를 했을 때 무처리 수준과 같았고, Ca, Mg, K는 7일 간격으로 혼합처리시 높은 경향을 보였으며, 토양관주를 했을 때 생육상태는 가장 우수하였지만, 수량은 대조구에서 383 ㎏ 10a?¹ 수준으로 가장 많았고, 토양관주 처리에서 대조구의 93.0~95.0% 수준을 보였다. The present study evaluated the effect of chitosan on the changes of soil chemical properties, soil microbial population, and yield of red pepper (Capsicum annuum L.) for eco-friendly agriculture in an upland field. We utilized four treatment groups, control, foliar spray, soil drench, and foliar spray + soil drench with chitosan, and analyzed these variations throughout the seven, fourteen, and twenty one days interval. The pH values, organic matter, and available phosphate in the upland soil at the harvesting stage decreased in the chitosan treatment. The populations of bacteria, actinomyces, and fungi in the upland field were increased in plots treated with chitosan. The chlorophyll content of leaves was no significant differences between the control and the chitosan treatments, while calcium content of leaves was significantly higher in the chitosan treatments than in the control. In addition, the nitrogen content of leaves was no significant differences between the foliar spray and the soil drench. The yield of red pepper was significantly higher in the control (383 ㎏ 10a?¹) than the chitosan treatments and the yield of soil drench with chitosan reached up to 95% of control.

Haemostatic chitosan coated gauze: in vitro interaction with human blood and in-vivo effectiveness

M. Pogorielov,O. Kalinkevich,V. Deineka,V. Garbuzova,A. Solodovnik,A. Kalinkevich,T. Kalinichenko,A. Gapchenko,A. Sklyar,S. Danilchenko 한국생체재료학회 2015 생체재료학회지 Vol.19 No.4

Background: Chitosan and its derivates are widely used for biomedical application due to antioxidative, anti-inflammatory, antimicrobial and tissue repair induced properties. Chitosan-based materials also used as a haemostatic agent but influence of different molecular weight and concentration of chitosan on biological response of blood cells is still not clear. The aim of this research was to evaluate interaction between human blood cells and various forms of chitosan-based materials with different molecular weight and chitosan concentration and prove their effectiveness on in-vivo model. Methods: We used chitosan with molecular weight 200, 500 and 700 kDa and deacetylation rate 80-82 %. For chitosan impregnation of gauze chitosan solutions in 1 % acetic acid with different concentrations (1, 2, 3, 5 %) were used. We used scanning electron microscopy to obtain information about chitosan distribution on cotton surface; Erythrocyte agglutination test and Complete blood count test – for evaluation of interaction between blood cells and chitosan-based materials with different compound. In-vivo studies was performed in 20 Wistar rats to evaluate effectiveness of new dressing. Results: Our data shown that chitosan can bind erythrocytes in concentration-depend manner that does not depend on its molecular weight. In addition, chitosan-based materials affect selectively human blood cells. Composition of chitosan with cotton materials does not change erythrocyte shape and does not cause agglutination. Conclusions: Сotton-chitosan materials have higher adhesive properties to platelets that depend on molecular weight and concentration of chitosan. These materials also change platelets’ shape that probable is one of the most important mechanisms of haemostatic effect. In-vivo studies have shown high effectiveness of 2 % 200 kDa chitosan for stop bleeding from arteries of large diameter.

해양식물인 김의 양식에 있어 키토산 처리 효과

김현진 ( Hyun Jin Kim ),문상미 ( Sang Mi Moon ),신종암 ( Jong Ahm Shin ),함경식 ( Kyung Sik Ham ) 한국키틴키토산학회 2003 한국키틴키토산학회지 Vol.8 No.2

생리활성 기능을 갖고 있으면서 지구상에 cellulose 다음으로 많이 존재하는 탄수화물인 chitin으로부터 쉽게 얻을 수 있는 chitosan의 김양식에 대한 처리효과를 조사하였다. 패각사상체 배양에 있어 패각에 chitosan을 처리하였을 때 처리하지 않은 패각에 비해 사상체의 부착이 현저히 높았다. 또한 30 cps, 90 cps chitosan을 처리한 후 비교하였을 때 30 cps chitosan 처리구의 사상체 부착율이 높은것으로 나타나 chitosan 종류에 따라 그 효과가 다름을 알 수 있었다. 이 결과는 채묘시 망에 chitosan 처리를 함으로써 망에 김 부착율을 높이고 결과적으로 다른 잡조류의 부착율을 떨어뜨릴 수 있다는 가능성을 보여주고 있다. 한편 실내 실험에서 엽체 배양에 chitosan을 처리한 결과 30 cps chitosan 처리구가 101.2%, 90 cps chitosan 처리구가 92.1%의 무게증가율을 보인 반면 control는 72.9%로 30 cps chitosan 처리구가 90 cps chitosan 처리구보다 9.1%, control 보다 28.3%의 증가율을 보였으며 90 cps chitosan 처리구도 comtrol 보다 19.2% 증가 하였으며 chitosan을 처리했을 때가 control 보다 훨씬 부드럽고 윤기가 좋았다. 이 결과는 김양식 중에 chitosan을 처리함으로써 수확량 증가와 품질개선효과도 볼 수 있다는 가능성을 보여주고 있다. Chitosan are known to stimulate growth and defense responses of many land-plants. This led us to study whether chitosan affects laver, which is a sea-plant. Here we report that chitosan treatment affects the growth of laver (Porphyra) and attachment of conchocelis to the oyster shells. The oyster shells coated with 1% chitosan (74 kD molecular mass) showed higher attachment and growth of conchocelis compared to the non-treated oyster shells and the shells coated with 1% chitosan of 110 kD. During the cultivation of Porphyra in the laboratory, treatments of Porphyra with 0.1% chitosans of 74 kD and 110 kD showed increments of weight by 28.3% and 9.1 %, respectively, compared to the control (non-treated) Prophyra. Furthermore, chitosan-treated Porphyra appeared to be softer and brighter than control.

감마선 조사에 의해 제조된 키토산/아크릴산 하이드로겔의 특성

심재운 ( Jae Woon Shim ),강필현 ( Phil Hyun Kang ),박성은 ( Sung Eon Park ),노영창 ( Young Chang Nho ) 한국공업화학회 2002 공업화학 Vol.13 No.7

키토산(chitosan)을 아크릴산에 용해시킨 후 개시제 혹은 가교제를 필요로 하지 않는 감마선을 이용해 약물 전달용 하이드로겔을 제조하고 그 특성을 조사하였다. 키토산과 아크릴산의 조성비를 각각 0.1∼1.5 wt%와 30, 50, 70 vol%로 혼합하여 30∼70 KGy의 조사량으로 감마선 조사하여 제조된 하이드로겔의 겔함량은 96% 이상이었으며, 키토산과 아크릴산의 비율이 증가할수록, 조사량이 증가할수록 99%까지 증가하였다. 제조된 겔의 팽윤도는 키토산과 아크릴산의 비율이 증가할수록, 조사량이 증가할수록 감소하였으며, 20 h 내에서 급격히 증가하다가 서서히 평형에 도달하였다. 또한, 감마선 조사로 제조된 chitosan/AAc 겔을 pH 1∼12에서 팽윤시킨 결과, 용액의 pH가 증가 할수록 팽윤도도 증가하였으며, pH 12에서 가장 큰 값을 나타내었다. 이러한 겔의 팽윤 특성은 감마선 조사에 의해 형성된 가교결합과 이온화된 amino기와 carboxyl기의 작용에 의한 것임을 FT-IR과 SEM 관찰로 확인할 수 있었다. The chitosan/acrylic acid (AAc) hydrogels for drug delivery were synthesized with polymerization of acrylic acid in the presence of chitosan mitiated by gamma-ray irradiation and their characteristics were investigated. Various chitosan/AAc hydrogels were prepared with the different ratios of chitosan (0.1∼1.5 wt%), acrylic acid (30, 50, 70 vol%) and radiation dose (30∼70 KGy). The gel contents of hydrogels were more than 96%, and as the ratios of chitosan/acrylic acid and radiation dose were increased, it increased to 99%. The swelling degrees of chitosan/AAc hydrogels decreased as the ratio of chitosan/acrylic acid and radiation dose increased, and reached equilibrium within 20 h. In the pH range of 1∼12, chitosan/AAc hydrogels were more swollen at high pH than low pH, and showed the largest swelling occurred at pH 12. FTIR as well as SEM results indicated that the swelling characteristics of chitosan/AAc hydrogels were due to the cross-linking of ionized amino and carboxylic functional groups by gamma irradiation.

α, β, γ-키틴으로 제조한 키토산의 특성

최혜영 ( Choe Hye Yeong ),김태형 ( Kim Tae Hyeong ),손소희 ( Son So Hui ),공병기 ( Gong Byeong Gi ),최창용 ( Choe Chang Yong ),김동곤 ( Kim Dong Gon ),장미경 ( Jang Mi Gyeong ),노홍균 ( No Hong Gyun ),나재운 ( Na Jae Un ) 한국키틴키토산학회 2004 한국키틴키토산학회지 Vol.9 No.1

본 연구에서는 천연자원으로부터 α-, β- 및 γ-chitin을 분리하였고, 이를 이용하여 α-, β- 및 γ-chitosan을 제조하였다. 원재료의 화학적 조성과 chitin과 chitosan의 일반성분을 분석하였으며 상대점도측정과 Kina 적정법을 이용하여 점도평균분자량과 탈아세틸화도를 측정하였고 FT-IR spectrophotometer, soild state CP/MAS ^(13)C NMR spectrophotometer 에 의해 α-, β- 및 γ-chitin과 chitosan의 제조를 확인하였다. α-, β- 및 γ-chitin의 각각의 분자량이 701, 612 그리고 524 kDa으로 측정되었으며 α-, β-및 γ-chitosan의 분자량이 603, 607, 329 kDa임을 확인하였다. α-, β-및 γ-chitin의 탈아세틸화도가 21.8%, 3?.3% 그리고 44.7%로 확인되었고 α-, β- 및 γ-chitosan의 탈아세틸화도가 97.1%, 99.2%, 그리고 96.6% 임을 확인하였다. Chitin의 FT-IR 스펙트럼에서 amide I에서의 흡수 밴드가 α-chitin에 있어서는 이중선으로, β-chitin에 있어서는 단일선으로 나타났으며 γ-chitin에서는 α-, β-chitin의 중간형태로 나타났음을 확인하였다. Chitosan의 FT-IR 스펙트럼에서 탈아세틸화 반응에 의해 amide Ⅰ과 amide Ⅱ의 흡수 피크가 현저히 감소하였음을 확인하였다. Chitin의 Solide state CP/MAS ^(13)C NMR 스펙트럼결과에서 α-chitin의 경우 C3과 C5의 피크가 각각 73과 75 ppm에서 나타났으며 β-chitin은 74 ppm에서 단일선으로 나타났고, γ-chitin의 경우 C3과 C5의 흡수피크가 α-chitin과 유사한 형태의 피크를 나타내었다. Chitosan의 Soilde state CP/MAS ^(13) NMR 스펙트럼에서 C1~C6가 잘 나타나 있고, 탈아세틸화 반응에 의해 메틸탄소 및 카르보닐 탄소가 거의 나타나지 않았다. α-, β- and γ-chitin were isolated from crab shell, squid pen, beetles cuticles by acid, alkali treatment and α-, β- and γ-chitosan were prepared from α-, β- and γ-chitin. Chemical compositions of raw materials and elemental of chitin and chitosan were analyzed. A weight-average molecular weight and degree of deacetylation (DDA) were determines by viscometry and Kina titration. Its structural characterization was analyzed by FT-IR spectrophotometer and solid state CP/MAS C NMR spectrophotometer. A molecular weight of α-, β- and γ-chitin were determined by viscometer resulting in 701. 612, and 524, kDa, respectively. A molecular weight of α-, β- and γ-chitosan were calculated with 603., 607 and 329 kDa, respectively. The DDA of α-, β- and γ-chitin were 21.8%, 32.3% and 44.7%, respectively. The DDA of α-, β- and γ-chitosan were 97.1%, 99.2% and 96.6%, respectively. At the FT-IR spectra of chitin, α-, β- chitin. And at the FT_IR sectra of chitosan, absorption band of amide I and amide H decreased because of the deacetylation of chitin, where as the absorption band of amine group was newly formed. From solid state CP/MAS C NMR spectra of chitin, two signals appeared at around 73 and 75 ppm assigned to C3 and C5 carbon atoms in α-chitin are sharply resolved, the signals of C3 and C5 in β-chitin shows singlet at around 74 ppm. In case of γ-chitin, two signals show at around 73 and 75 ppm assigned to C3 and C5 carbon atoms. From solid stat CP/MAS C NMR spectra of chitosan, the carbon of the C1-C6 positions were cleared identified and peaks of CH_3 and C=0 decreased significantly because of the deacetylation

Chitosan Coating 이 계란이 저장중 품질에 미치는 영향

이신호,노홍균,정영희 대구효성가톨릭대학교 식품과학연구소 1996 식품과학지 Vol.8 No.-

Chitosan의 막형성능을 이용하여 계란의 저장성 증진 효과를 검토하기 위하여 세척란과 미세척란을 chitosan용액 1%와 2%를 이용하여 coating 처리하여 20℃에서 저장하면서 난중 감소율, yolk index, Haugh unit 및 유화력 등을 측정하여 대조구와 비교한 결과 난중 감소율은 chitosan 처리에 의해 저장 기간이 경과함에 따라 감소하는 경향을 나타내었다. Yolk index와 Haugh unit 측정결과 chitosan의 처리 효과는 p<0.05 수준에서 유의성이 인정되었다. 난황의 유화력은 저장 기간이 경과함에 따라 감소하였으며 chitosan 처리구는 대조구에 비해 완만하게 감소하였다. Chitosan coating의 농도별 처리는 계란의 저장중 무처리구에 비해 품질보존효과가 점차 뚜렷하였으며, 미세척란 보다 세척란에서 더욱 뚜렷하였다. Weight loss, yolk index, Haugh unit, and emulsifying capacity of egg were measured to evaluate the effect of chitosan coating on egg quality during storage at 20℃. Reduction ratio of egg weight was 7.04% for chitosan-uncoated egg, 6.46% for 1% chitosan-coated egg, and 5.43% for 2% chitosan-coated egg after 60 days storage, respectively. Yolk index and Haugh unit of chitosan-coated or -uncoated egg were significantly different. Emulsifying capacity of egg yolk was less decreased in chitosan-coated egg than in chitosan-uncoated egg during storage. It was also found that quality of chitosan-coated egg was much better than that of uncoated egg during storage at 20℃. Effect of chitosan coating on egg quality was more apparent in washed egg than in unwashed egg.

Chitosan 이 난황의 유화력에 미치는 영향

이신호 대구효성가톨릭대학교 식품과학연구소 1996 식품과학지 Vol.8 No.-

난황을 이용한 유화식품인 마요네즈 제조시 천연고분자 물질인 chitosan의 사용 가능성을 조사하기 위하여 난황의 유화력과 유화 안정성에 미치는 chitosan의 효과와 마요네즈 제조시 chitosan 첨가 특성을 조사하였다. Chitosan을 난황 무게에 대해 0.1% 첨가한 결과 난항의 유화력은 약 10% 증진되었으며 유화 안정성은 뚜렷이 증진되었다. 마요네즈 제조시 0.1% chitosan 첨가에 의해 마요네즈의 유화 안정성은 증가되었으며 대조구의 첨가 대두유에 비해 40% 중량 처리구에 있어 유화 안정성 및 점도는 대조구에 비해 우수하였으며 색택은 비슷한 경향을 나타내었다. 마요네즈의 퍼짐성, 점성, 맛, 종합적 기호도에 관한 기호성은 0.1% chitosan 첨가구가 대조구에 비해 양호하였으며, 40% 증량한 경우 더욱 우수하였으며 60% 증량한 경우 대조구와 유사하였다. Effects of chitosan on emulsifying capacity and emulsifying stability of egg yolk were examined to utilize chitosan in the preparation of mayonnaise. Emulsifying capacity of egg yolk increased about 10% by the addition of 0.1% chitosan, but an increase in the concentration of chitosan from 0.1% to 0.2% did not show a significant difference. Emulsion stability and viscosity of mayonnaise were enhanced by the addition of 0.1% chitosan but color was similar to control. The effect of chitosan on the emulsion stability and viscosity of mayonnaise was decreased as oil concentration was increased above 40% compared to control. Mayonnaise prepared by the addition of 0.1% chitosan and 40% more oil than control showed enhancement in sensory quality such as dispersibility, viscosity, taste and overall acceptability compared with control and 0.1% chitosan added mayonnaise.

Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials

Lee, Jue-Yeon,Nam, Sung-Heon,Im, Su-Yeon,Park, Yoon-Jeong,Lee, Yong-Moo,Seol, Yang-Jo,Chung, Chong-Pyoung,Lee, Seung-Jin 梨花女子大學校 藥學硏究所 2002 藥學硏究論文集 Vol.- No.11

For the pulpose of obtaining high bone forming efficacy. developlment of chitosan was attempted as a tool useful as ascaffolding device. Porous chitosan matrices, chitosan-poiy(L-lactide) (PLLA) composite matrtices and chitosan coated onPLLA matrices were dealt with in this research. Porous chitosan matrix was fabricated by freeze-drying and cross-linkingaqueous chitosan solution. Porous chitosan matrix combined with ceramics and constituents of extracellular matrices wereprepared and examined for their bone regenerative potential. Composite porous matrix of chitosall-PLLA was prepared bymixing polyiactide with chitosan and freeze-drying. Al1 chitosan based devices demonstrated improved bone formingcapacity by increasing mechanical stability and biocompatibility. Release of platelet-derived growth factor-BB (PDGf-BH )from these matrices exerted significant osteoinductive effect in addition to the higf esteocdElducting capacity of the porouschitosan matrices. The hydrophobic surface of PLLA matrices was modified by chitosan to enhance cell affinity andwettability, The chitosan coafed PLLA matrix induced increased osteoblast attachment as compared with intact PLLAsurface. Overall results in this study demonstrated the usefulness of chitosan as drug releasing scaffolds and as modificationtools for currently used biomaterials to enhance tissue regeneration efficacy. These results may expand the feasibilitr ofcombinatfve strategy of controlled locai drug delivery concept and tissue engineered bone formation in reconstructivetherapy in the field of periodontics, orthopedics and plastic surgery.