Comparative transcriptome analysis reveals differential transcription in heat-susceptible and heat-tolerant pepper (Capsicum annum L.) cultivars under heat stress

Tao Li,Xiaowan Xu,Ying Li,Hengming Wang,Zhiliang Li,Zhenxing Li 한국식물학회 2015 Journal of Plant Biology Vol.58 No.6

Heat stress is an important agricultural problem around the world. In pepper (Capsicum annum L.), heat stress seriously affects pollination and yield. However, to date, the molecular basis of heat stress has not been extensively studied. Using the HiSeq™ 2000 sequencing platform, the seedling transcriptome of heat-susceptible C.annuum ‘S590’(CaS) and heat-tolerant ‘R597’ (CaR) under the heat stress was examined. Over five million clean reads were generated from each library, each corresponding to a coverage of >250,000 nt. About 73% of the reads were mapped to the pepper genome, and 3,799 and 4,010 differentially expressed genes (DEGs) were identified in ‘R597’ (CaR) and ‘S590’(CaS), respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses determined that the identified DEGs were involved in heat shock protein, heat shock transcription factors, hormone, as well as calcium and kinase signaling. Further validation identified 35 genes that were involved in stress response, and that most of the heat shock proteins were upregulated in two genotypes, and highly expressed in susceptible S590 than in tolerant cultivar R597; the transcription factors and hormone signaling genes showed higher levels of expression in the heat-tolerant cultivar R597 than that observed in the heat-susceptible S590. These findings facilitate in better understanding of the molecular mechanism underlying heat stress in different pepper genotypes.

Identification of Primary Failure Modes of Tunnel System and Influence of Supporting Structures on Tunnel System Reliability using Multiple Response Surfaces

Zhiliang Liu,Liang Li,Guangming Yu,Jun Hu,Houlai Lin,Chunli Li,Shibao Lu,Kaiqi Meng,Jun Lei 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

A method of calculating the failure probability of the tunnel system combining finite element numerical simulation with multiple response surface method (MRSM) and Monte-Carlo simulation (MCS) is presented. The applicability of the proposed methodology is verified through a subway interval soft rock tunnel in Qingdao, China. The sensitivity of Monte-Carlo sampling number and coefficients of variation for rock masses on the failure probability of tunnel system is conducted. The primary failure modes in tunnel system are identified by reanalyzing the failure samples. The simulation results demonstrate that the failure probability of a tunnel system within soft upper and hard lower surrounding rock mass is mainly attributed to the soft upper part of the surrounding rock. The coefficients of variation of the elastic modulus E1 and the internal friction angle ϕ1 of the pebble layer (soft upper part) have significant effect on the failure probability. The failure probability of tunnel system increases as the coefficients of variation of E1 and ϕ1 increase. Two primary failure modes are found to contribute to the tunnel system reliability. The effect of rock bolt length L and pipe-roof thickness H on tunnel system reliability and two primary failure modes as well are investigated. The simulation results indicate that both the enhancements in L and H tend to be more effective than the enhancement in either L or H if a small target failure probability of tunnel system is expected. The supporting structures design can be performed based on the potential sets of (L, H) satisfying target failure probability.

Melting Cell Based Compensated Design Method for Improving Dimensional Accuracy of Additively Manufactured Thin Channels

Li Sun,Xiaobo Ren,Jianying He,Zhiliang Zhang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.2

Powder-bed fusion additive manufacturing technology makes it possible to produce parts with complicated geometry and high accuracy. However, dimensional deviation caused by powder overmelting and dross formation is still a challenge for manufacturing thin channels. In this study, the origins of the overmelting of printed thin channels were analyzed and a concept called “melting cell” is proposed to describe and quantify the geometric error. Based on the geometrical relationship between the melting cell and target channel, a method for predicting and optimizing the final geometry of thin channels is outlined. In order to verify the method, geometries of thin horizontal circular channels in various sizes are studied as examples. The predicted results by the proposed method show a remarkable agreement with available experimental results. Moreover, a new egg-shaped compensated design, which is able to improve the dimensional accuracy of thin horizontal circular channels, is presented. The proposed method is simple yet very effective. It can be easily extended to the manufacturing of thin channels with various shapes, materials, and different powder bed fusion processes.

Highly catalysis Zinc MOF-loaded nanogold coupled with aptamer to assay trace carbendazim by SERS

Jingjing Li,Aihui Liang,Jinling Shi,Zhiliang Jiang Techno-Press 2023 Advances in nano research Vol.14 No.4

Zinc metal organic framework (MOF_Zn)-loaded goad nanoparticles (AuNPs) sol (Au@MOF_Zn), which was characterized by TEM, Mapping, FTIR, XRD, and molecular spectrum, was prepared conveniently by solvothermal method. The results indicated that Au@MOF_Zn had a very strong catalytic effect with the nanoreaction of AuNPs formation between sodium oxalate (SO) and HAuCl4. AuNPs in the new indicator reaction had a strong resonance Rayleigh scattering (RRS) signal at 370 nm. The indicator AuNPs generated by this reaction, which had the most intense surface enhanced Raman scattering (SERS) peak at 1621 cm -1. The new SERS/RRS indicator reaction in combination with specific aptamer (Apt) to fabricate a sensitive and selective Au@MOF_Zn catalytic amplification-aptamer SERS/RRS assay platform for carbendazim (CBZ), with SERS/RRS linear range of 0.025-0.5 ng/mL. The detection limit was 0.02 ng/mL. Similarly, this assay platform has been also utilized to detect oxytetracycline (OTC) and profenofos (PF).

Analysis on Association of a SNP in the Chicken OBR Gene with Growth and Body Composition Traits

Wang, Ying,Li, Hui,Zhang, YuanDan,Gu, ZhiLiang,Li, ZhiHui,Wang, QiGui Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.12

Leptin receptor (OBR) is a member of the class I cytokine receptor family. It signals mainly via the JAK/STAT pathway and plays an important role in regulating body energy storage and metabolism. This study was designed to investigate the effects of the OBR gene on chicken growth and body composition. Broiler lines selected divergently for or against abdominal fat were used. Primers for the exon9-region in the OBR gene were designed using chicken genomic sequences from the public genome domain. A C/A single nucleotide polymorphism (SNP) was found and its three genotypes (AA, AB and BB) were identified in this population. The results showed that the OBR polymorphism was associated with fatness traits, such as abdominal fat weight and abdominal fat percentage. This research suggests that OBR or a linked gene has effect on fat deposition in the chicken.

Complete chloroplast genome sequence and phylogenetic analysis of Rhododendron molle G. Don, an endangered Ericaceae species located on Dabie Mountains (central China)

Xu Bilin,Li Zhiliang,Liu Yan,Zhang Wanjing,Yu Jiaojun,Dong Hongjin,Zhang Jialiang,Wang Shuzhen 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2

Rhododendron molle G. Don, an endemic species of the Ericaceae family, possesses valuable medicinal and horticultural values. In this research, R. molle cp genome proved to be a typical quadripartite structure with the length of 200,878 bp. In particular, the lengths of large single-copy region (LSC), small single-copy region (SSC), and inverted repeat regions (IR) were 198,019 bp, 629 bp, and 1117 bp, respectively. Among the 149 unique genes, 97 were protein-coding genes, 44 were tRNA genes, and 8 were rRNA genes, respectively. Leucine was the most representative amino acid (10.663%), while Cysteine was the lowest representative (1.178%). A set of 30 codons showed obvious codon usage bias, while 29 were A/U-ending codons. Six gene regions showed high levels of nucleotide diversity (Pi > 0.02). Totally, 273 SSRs were identified. Maximum-likelihood (ML) phylogenetic analysis revealed that R. molle was relatively closed to the R. pulchrum and R. delavayi. High similarity was detected among Ericaceae species, and the coding regions were more conserved than the non-coding regions. Expansion and contraction detected in IR region could be the main length variation in R. molle and related Ericaceae species. This research will supply rich genetic resource for R. molle and related species of Ericaceae.

An IPD-based Incentive Mechanism to Eliminate Change Orders in Construction Projects in China

Jiankun Ma,Zhiliang Ma,Jiulin Li 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.7

As in other countries where DBB (Design-Bid-Build) is used, one of the major problems in construction projects in China is cost overrun, and its main cause is change orders that occur during the construction phase. As a new mode of project delivery, IPD (Integrated Project Delivery) is considered capable of solving major problems including change orders in construction projects by involving contractors in the design phase to optimize design results and by introducing an incentive mechanism through which cost savings are shared among participants. However, the existing incentive mechanisms are difficult to adapt in China because contractors rely on change orders to obtain profit from construction projects. This research aims to establish an IPD-based incentive mechanism to eliminate change orders in construction projects in China. First, the final account statement of a completed construction project was analyzed to obtain the economic law of change orders. Second, based on this economic law, this research established an incentive mechanism composed of a compensation method for contractors, another compensation method for designers and a method for calculating their parameters. Finally, 21 completed construction projects and a new construction project were used to validate the incentive mechanism. This research provides not only an applicable IPD-based incentive mechanism to eliminate change orders in construction projects in China but also provides an approach for applying IPD in any other country where DBB is used and where the existing IPD incentive mechanisms cannot be directly applied.

Synthesis of Rod-Like Porous MgFe2O4 Architectures as a Catalyst for Ammonium Perchlorate Thermal Decomposition

Run Chen,Gang Li,Weiyang Bai,Shuang Bao,Zhiliang Cheng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.6

The development of highly active catalysts for the pyrolysis of ammonium perchlorate (AP) is of considerable importance for AP-based composite solid propellant. In the present study, we produced porous MgFe2O4 architectures by using a facile two-step strategy. A rod-like precursor of MgFe2(C2O4)3 · nH2O (diameter: 0.5–2.5 μm; length: 2–15 μm) was fabricated under solvothermal conditions using metal sulfates as raw materials and oxalic acid as the precipitant. Subsequently, porous MgFe2O4 architectures were obtained by the thermal treatment of the as-prepared oxalate precursor, during which the mesopores were formed in situ via the liberation of volatile gases, while the rod-like morphology was well preserved. The catalytic performances of the as-synthesized porous rod-like MgFe2O4 architectures with respect to the AP pyrolysis were assessed using differential scanning calorimetry (DSC) techniques. The results indicated that the high thermal decomposition temperature and the apparent activation energy of AP with 2 wt.% MgFe2O4 addition decreased from 445.4 ℃ to 386.7 ℃ and from 280.5ffi11.8 to 147.6ffi4.8 kJ mol-1, respectively. Meanwhile, the decomposition heat of AP with MgFe2O4 as the additive reached up to 1230.6 J g-1, which was considerably higher than that of its neat counterpart (695.8 J g-1). Thus, porous rod-like MgFe2O4 architectures could be served as the catalyst for the AP pyrolysis.

Simulation and experiment of secondary contact stiffness of rough surface

Guang Zhao,Yingjie Li,Zexin Zhang,Zhiliang Xiong,Shengxiang Li,Mengru Wang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.3

Machined surfaces usually need to be loaded several times during assembly. The surface morphology after the simulation loading may be quite different from the physical loading, which subsequently causes differences between the contact stiffness after secondary simulation loading and secondary physical loading results. The fractal theory was used to reconstruct the measured surface morphology, followed by contact stiffness simulation and test. The results show that contact stiffness of new morphology obtained by the first simulated loading using the elastic-plastic deformation theory are smaller than the first loading test results, with an average error of -9.71 %. Contact stiffness of used morphology obtained by the first simulated loading using the pure elastic deformation theory are in good agreements with the second loading test results, with an average error of 4.71 %. However, the average contact stiffness of new morphology obtained by the second simulated loading using the elastic-plastic deformation theory, which is traditionally used for contact analysis, are 2.15 times larger than the second loading test result. These research results demonstrate a feasible finite element method (FEM) for solving the contact problem of rough surfaces that need to be assembled multiple times.

A new peptide-mediated COF nanocatalytic amplification SERS quantitative assay for ultratrace Cu2+

Yiyi Shu,Shengfu Zhi,Sha Li,Aihui Liang,Zhiliang Jiang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

Peptides are an important recognition element for small molecules and have been used in the field of biologicalanalysis. However, it has been rarely reported that a novel method combining peptides as recognitionelements with covalent organic framework (COF) as catalytic amplification signal to constructhighly sensitive and selective three-mode molecular spectroscopy. In this paper, high catalytic and stablethree COFs were prepared, and the catalysis of the new indicator nanoreaction of HAuCl4-sodiumformate(Fo) was studied by molecular spectral slope procedure. The produced gold nanoparticles (AuNPs) exhibita strong resonance Rayleigh scattering (RRS) peak at 370 nm and surface plasmon resonance absorption(Abs) peak at 540 nm. In the presence of molecular probes, a strongest surface-enhanced Raman scattering(SERS) peak was generated at 1617 cm1. Combining the COFTpBD amplification indicator reactionwith specific peptide (PT) reaction of Cu2+, a novel, sensitive and selective SERS/RRS/Abs assay platformwas established, with a SERS linear range of 0.005–0.115 nmol/L Cu2+. In addition, the two workingcurves of lg[Cu2+] vs SERS and [Cu2+] vs SERS intensity, and the nanocatalytic mechanism were discussed.