Myricitrin exerts protective effect on retina in diabetic retinopathy via modulating oxidative stress expression of VEGF and apoptosis in experimental rats: a docking confirmation study

Jiang Jing,Che Xin,Qian Yiwen,Lu Shiheng,Wang Zhiliang 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.2

Background The present study investigates the protective effect of myricitrin (MCT) on the retinal tissues in diabetic retinopathy of diabetic rats. Objective The diabetic rat model was created by treating the rats with streptozotocin along with high-energy diet. The blood glucose was evaluated by glucose estimation kit, and rats measuring glucose ≥ 12.00 mmol/L were considered diabetic and were selected for the study. The diabetes-induced rats were divided randomly as diabetic control rats (vehicle treated) (DB), diabetic rats MCT (100 mg/kg) (DB + MCT 100 mg/kg) and diabetic rats MCT 200 mg/kg (DB + MCT 200 mg/kg). Rats received MCT for 12 weeks via oral route daily. The rats after 12 weeks were sacrificed, and the eyes were removed. The retina tissue was evaluated for thickness, oxidative stress markers, levels of VEGF and Bax and Bcl-2. In silico docking analysis was done by Autodock Vina tools for target confirmation of MCT. Results MCT decreased the serum glucose levels and also improved the body weight in diabetic rats. MCT improved thickness and apoptosis of cells. It was also found that MCT decreased oxidative stress, and improved levels of VEGF in retinal tissues of diabetic rats. MCT also exerted anti-apoptotic activity as shown by over-expression of Bcl-2 and suppression of Bax in retinal tissues of rats. Docking study confirmed potential binding affinity of MCT with VEGF. Conclusions MCT could be a potential therapeutic molecule in treating diabetic retinopathy which could modulate glucose levels, oxidative stress and levels of VEGF in diabetic rats.

Facile Preparation of ZnO Nanocatalysts for Ozonation of Phenol and Effects of Calcination Temperatures

Yuming Dong,Hui Zhao,Zhiliang Wang,Guangli Wang,Aizhen He,Pingping Jiang 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.1

ZnO nanoparticles were synthesized through a facile route and were used as ozonation catalysts. With the increase of calcination temperature (150-300 oC), surface hydroxyl groups and catalytic efficiency of asobtained ZnO decreased remarkably, and the ZnO obtained at 150 oC showed the best catalytic activity. Compared with ozonation alone, the degradation efficiency of phenol increased above 50% due to the catalysis of ZnO-150. In the reaction temperatures range from 5 oC to 35 oC, ZnO nanocatalyst revealed remarkable catalytic properties, and the catalytic effect of ZnO was better at lower temperature. Through the effect of tertbutanol on degradation of phenol and the catalytic properties of ZnO on degradation of nitrobenzene, it was proposed that the degradation of phenol was ascribed to the direct oxidation by ozone molecules based on solidliquid interface reaction.

Myotomy of Distal Esophagus Influences Proximal Esophageal Contraction and Upper Esophageal Sphincter Relaxation in Patients with Achalasia After Peroral Endoscopic Myotomy

( Yutang Ren ),( Xiaowei Tang ),( Fengping Chen ),( Zhiliang Deng ),( Jianuan Wu ),( Soma Nei ),( Bo Jiang ),( Wei Gong ) 대한소화기기능성질환·운동학회 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.1

Background/Aims The motility change after peroral endoscopic myotomy (POEM) in achalasia is currently focused on lower esophageal sphincter (LES). This study aims to investigate the correlation of motility response between distal and proximal esophagus after POEM. Methods A total of 32 achalasia patients who received POEM and high-resolution manometry (HRM) were included for analysis. Eckardt score was used to assess symptom improvement. HRM was applied for studying motility. Main parameters analyzed were (1) LES: resting pressure (restP), 4-second integrated relaxation pressure; (2) esophageal body (EB): contractile integral of distal segment with myotomy (CI-DM) and proximal segment without myotomy (CI-PNM); and (3) upper esophageal sphincter (UES): relaxation pressure (UES-RP). Results There were 6 type I, 17 type II, and 9 type III achalasia patients included for analysis. (1) Eckardt score, LES tone, CI-DM, CI-PNM and UES-RP were reduced remarkably after POEM (P < 0.001). (2) no significant correlation was noted between LES tone and contractile intergral of EB. (3) a positive linear correlation of CI-DM and CI-PNM changes was detected (P < 0.001). (4) the change of UES-RP was positively correlated with the change of contractile integral of EB (P < 0.001). Conclusions Myotomy of the distal esophagus would attenuate proximal EB contraction and assist UES relaxation in achalasia patients after POEM. (J Neurogastroenterol Motil 2016;22:78-85)

Facile Preparation of ZnO Nanocatalysts for Ozonation of Phenol and Effects of Calcination Temperatures

Dong, Yuming,Zhao, Hui,Wang, Zhiliang,Wang, Guangli,He, Aizhen,Jiang, Pingping Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.1

ZnO nanoparticles were synthesized through a facile route and were used as ozonation catalysts. With the increase of calcination temperature ($150-300^{\circ}C$), surface hydroxyl groups and catalytic efficiency of asobtained ZnO decreased remarkably, and the ZnO obtained at $150^{\circ}C$ showed the best catalytic activity. Compared with ozonation alone, the degradation efficiency of phenol increased above 50% due to the catalysis of ZnO-150. In the reaction temperatures range from $5^{\circ}C$ to $35^{\circ}C$, ZnO nanocatalyst revealed remarkable catalytic properties, and the catalytic effect of ZnO was better at lower temperature. Through the effect of tertbutanol on degradation of phenol and the catalytic properties of ZnO on degradation of nitrobenzene, it was proposed that the degradation of phenol was ascribed to the direct oxidation by ozone molecules based on solidliquid interface reaction.

Highly catalysis Zinc MOF-loaded nanogold coupled with aptamer to assay trace carbendazim by SERS

Jingjing Li,Aihui Liang,Jinling Shi,Zhiliang Jiang Techno-Press 2023 Advances in nano research Vol.14 No.4

Zinc metal organic framework (MOF_Zn)-loaded goad nanoparticles (AuNPs) sol (Au@MOF_Zn), which was characterized by TEM, Mapping, FTIR, XRD, and molecular spectrum, was prepared conveniently by solvothermal method. The results indicated that Au@MOF_Zn had a very strong catalytic effect with the nanoreaction of AuNPs formation between sodium oxalate (SO) and HAuCl4. AuNPs in the new indicator reaction had a strong resonance Rayleigh scattering (RRS) signal at 370 nm. The indicator AuNPs generated by this reaction, which had the most intense surface enhanced Raman scattering (SERS) peak at 1621 cm -1. The new SERS/RRS indicator reaction in combination with specific aptamer (Apt) to fabricate a sensitive and selective Au@MOF_Zn catalytic amplification-aptamer SERS/RRS assay platform for carbendazim (CBZ), with SERS/RRS linear range of 0.025-0.5 ng/mL. The detection limit was 0.02 ng/mL. Similarly, this assay platform has been also utilized to detect oxytetracycline (OTC) and profenofos (PF).

A new peptide-mediated COF nanocatalytic amplification SERS quantitative assay for ultratrace Cu2+

Yiyi Shu,Shengfu Zhi,Sha Li,Aihui Liang,Zhiliang Jiang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

Peptides are an important recognition element for small molecules and have been used in the field of biologicalanalysis. However, it has been rarely reported that a novel method combining peptides as recognitionelements with covalent organic framework (COF) as catalytic amplification signal to constructhighly sensitive and selective three-mode molecular spectroscopy. In this paper, high catalytic and stablethree COFs were prepared, and the catalysis of the new indicator nanoreaction of HAuCl4-sodiumformate(Fo) was studied by molecular spectral slope procedure. The produced gold nanoparticles (AuNPs) exhibita strong resonance Rayleigh scattering (RRS) peak at 370 nm and surface plasmon resonance absorption(Abs) peak at 540 nm. In the presence of molecular probes, a strongest surface-enhanced Raman scattering(SERS) peak was generated at 1617 cm1. Combining the COFTpBD amplification indicator reactionwith specific peptide (PT) reaction of Cu2+, a novel, sensitive and selective SERS/RRS/Abs assay platformwas established, with a SERS linear range of 0.005–0.115 nmol/L Cu2+. In addition, the two workingcurves of lg[Cu2+] vs SERS and [Cu2+] vs SERS intensity, and the nanocatalytic mechanism were discussed.