Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

커패시터 放電 임펄스 着磁機 및 着磁 요크시스템의 定數算定에 관한 硏究

白壽鉉,金 龍,金必洙,尹秀鳳,朴永浩,尹信容 동국대학교 대학원 1992 大學院硏究論集-東國大學校 大學院 Vol.22 No.-

In a capacitor-discharge impulse magnetizer, a magnet is magnetized by the discharging current of capacitor The actual construction of magnetizing fixtures and discharge matching is expensive and time-consuming. Since the parameters of the impulse magnetizer have been determined and the resistance and inductance of such a magnetizing fixture can be easily computed using FEA, it is possible to predict the performance of such a fixture without actually building it. This paper describes that by mathematically modeling the capacitor-discharge impulse magnetizer fixture the problem can be solved with resonable predictability. The basic equations are presented and used to predict the magnetic-field intensity inside the fixtures. Finite-Element-Analysis is also used as part of the design process

경희대학교 수원캠퍼스 노천극장 주변 공원 기본계획

김동찬,이윤수,권오원,민창기,김태현,박경모 경희대학교 디자인연구원 2004 예술· 디자인학연구 Vol.7 No.1

We, Kyung-hee University in Suwon has planned to build an open-air theater behind the building of natural science, and feel the need of creating places for cultural life and recreation. As the origin aim of this construction and landscape, university students can use this facilities and place for their daily relaxation and cultivation of emotion, and they also can utilize them for places of their meetings and events. Concerning the following completion of the UN Peace Park, our theater and the surrounding places will expectingly play important roles for the park because the open-air No-cheon theater is included in the full site of the UN Peace park. The focused elements of this basic plan are maintaining the basic plan of the UN Peace Park, forming the visually creative background, of the open-air theater cultural and recreational places, balancing and integrating human, nature, and the useful facilities.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

민간에서 주로 사용되는 약용식물들 중 간독성 의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화합물의 불완전 연소및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성발현에 미치는 자근 수침액의 예방 및 치료 효과를 실험한 결과 자근 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고 B(a)P 투여로 증가한 혈청 total cholestelof 및 phospholipid 함량 역시감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the phamacologicalactivities of crude Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine aspartate aminotransferase(ALT,AST) and lipid composition indicated that Lithospermi Radix water extract sho-wed significant liver-protective activities against benzo(a)pyrene-induced hepato-toxicity.

Butane gas가 흰쥐 혈청과 조직의 Lactatedehydrogenase 및 Cholinesterase에 미치는 영향

윤수홍,박은주,조수열,최현태 한국환경독성학회 1991 환경독성보건학회지 Vol.6 No.2

Acute poisoning with organic solvents and other volatile compounds now usually follows deliberate inhalation (volatile substance abuse) or ingestion of these compounds. The effect of butane gas inhalation was analyzed for serum, liver, brain, lung and muscle. And the observations are revealed on rat cholinesterase activity, lactatedehydrogenase activity and electrophoretic pattern of lactatedehydrogenase isozyme. The results are as follows: 1. The rat cholinesterase activity on serum, liver and muscle show the decreased by increasing of inhalation time of butane gas in particular the lung cholinesterase activity was greatly affected. 2. Butane gas inhalation brought out the lactatedehydrogenase activity increased of the serum and the tissues and had an important effect especially in both the liver and muscle lactatedehydrogenase activities. 3. Each tissue was found to have a characteristic distribution of lactatedehydrogenase isozymes on celluloseacetate electrophoresis and the development of inhalation time is shown the disappearance and diffusion of band. The toxicity of butane gas inhalation was most prominence in the liver and lung toxicity was occurred also.

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호효과가 우수한 약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험 과정으로 탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene 으로 유도한 rats 의 간독성 발현에 미치는 자근 수침액의 예방 및 치료효과를 실험한 결과 자근 수침액의 투여는 B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고, B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량 역시 감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of cn de Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine·aspartate aminotransferase(ALT, AST) and lipid composition indicated that Lithospermi Radix water extractshowed significant liver-protective activities against benzo(a)pyrene-induced he-patotoxicity.

흡입물질의 위생학적 연구 : 본드 흡입이 흰쥐의 폐 및 기타 장기의 효소 활성에 미치는 영향 The Effect of Bond Inhalation on the Enzyme Activity of Lung and Other Organs in Rat

윤수홍,박은주,이주영,서혜령 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

Toluene, alcohol및 vinylester류 등을 함유하고 있는 bond를 이용하여 휘발성 물질이 생체에미치는 영향을 관찰하기 위하여 rat에 일정 농도의 bond를 일정 시간 흡입시킨 뒤 각 조직에대하여 효소의 생화학적 시험과 celluloseacetate electrophoresis를 실시하여 다음과 같은 결과를얻었다. 1. Bond 흡입은 시간이 증가함에 따라 혈청, 심장, 신장, 근육, 뇌의 ChE 활성을 억제하였으며특히 폐와 간장의 ChE활성에 가장 큰 영향을 나타내었다. 2. Bond 흡입에 의해 혈청 및 각 조직 중에서 LDH활성이 증가되었으며 특히 폐와 심장,근육에서 그 영향이 현저히 나타났다. 3. Celluloseacetate electrophoresis를 통한 각 조직의 LDH isozyme pattern에는 차이가 나타났으며 bond 흡입에 의해 band의 소멸과 선명도의 감소가 일어 났는데 특히 폐에 대한 독성이가장 현저했으며 근육에 대한 독성도 큰 것으로 나타났다. Acute poisoning with organic solvents and other volatile compou-nds now usually follows deliberate inhalation(volatile substance abuse) or inges-tion of these compounds. The effect of bond inhalation was analyzed for serum, liver, lung, brain,heart, kidney and muscle. And the observations are revealed on rat cholinesterase activity, lactatedeh-ydrogenase activity and electrophoretic pattern of lactatedehydrogenase isozyme. The results are as follows ; 1. The rat cholinesterase activity on serum, heart, muscle, kidney and brain showthe decrease by increasing of inhalation time of bond, in particular the lungand liver cholinesterase activity was greatly affected. 2. Bond inhalation brough out the lactatedehydrogenase activity increased ofthe serum and the tissues and had an important effect especially in both thelung and muscle lactatedehydrogenase activities. 3. Each tissue was found to have a characteristic distribution of lactatedehydro-genase isozymes on celluloseacetate elelctrophoresis and the development ofinhalation time is shown the disappearence and diffusion of band.

메치실린 내성 포도상구균에 대한 SK-302의 시험관내 및 생체내 효과

박윤선,김수기,신운섭,최선주,정종순,이종수 關東大學校醫科大學醫科學硏究所 1998 關東醫大學術誌 Vol.2 No.1

SK-302 is the active compound purified from culture filtrate of a streptomyces species (streptomyces sp. 302). The in vitro and in vivo antibacterial activities of SK-302 were evaluated against 6 clinically isolated MRSA strains and compared with the activities of vancomycin. SK-302 had node in virto antistaphylocoal activity stronger than that of vancomycin exhibiting MIC's, while vancomycin showed very weak 4 to 8fold lower than that of vancomycin. In SK-302 showed killing activity at the MIC, so SK-302 was superior to vancomycin. Furthermore, in acute systemic infections in mice, the ED_50s of SK-302 against infections by high-resistance MRSA were 30 to 40-feold less than that of vancomycin, therefore SK-302 showed potent in vive therapeutic efficacy against MRSA infection, These results suggest that SK-302 has excellent in virto in and in vivo activities against high-resistance MRSA.

열대 지역에서 통일형 초다수성 벼 품종의 생육 및 수량성 비교

이지윤,준현,성환,손영보,송유천,한상익,박동수,권영업,이철원,이종희 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2