임상병리검사업무의 전산화에 관한 연구

이승국,유영선 대한임상병리사협회 2001 대한임상병리사회지 Vol.21 No.1

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

자연 치관과 포세린 치관상에서 교정용 브라켓 부착시 전단 결합 강도와 파절 양상에 관한 비교 연구

이현선,김종수,유승훈 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

치아 우식증과 침식증의 진단과 본 연구는 교정용 밴드 사용의 대안으로 제시된 직접 부착 술식이 보철 치료를 받은 치아상에서도 적절한 결합 강도를 갖으면서 임상적으로 유용하게 사용될 수 있는지를 평가하고,불산과 포세린 전처리제의 사용 여부에 따른 결합 강도와 접착 파절 양상의 차이에 대해 알아보고자 시행하였다. 사람의 상하악 소구치 치관 20개를 대조군으로, 소구치의 협면을 복제한 포세린 치관 80개를 실험군으로 나눈 후,대조군은 37% 인산으로 산부식한 후 Transbond XT^(??)를 이용하여 금속 브라켓을 접착하였고,실험군들은 모두 포세린 치관에 통상적으로 sandblasting 처리를 하되,9.6% 불산으로 4분간 부식처리를 함께 한 군,포세린 전처리제를 적용한군,마지막으로 sandblasting과 불산 그리고 포세린 전처리제를 모두 적용한 군으로 20개씩 나눈 후 금속 브라켓을 접착하였다. 각 군간 전단 결합 강도와 접착 파절 양상을 비교하여 관찰한 결과,포세린 치관에 금속 브라켓을 접착할 시,기존의 비심미적인 밴드를 사용하지 않더라도 sandblasting과 불산 그리고 포세린 전처리제를 이용한 직접 부착 술식으로 임상적으로 유용하게 사용될 수 있음을 알 수 있었다. Orthodontic brackets often need to be bonded to porcelain such as porcelain fused to metal crowns and porcelain jacket crowns. The purpose of this study was to evaluate the clinical usability of direct bonding system on porcelain teeth by measuring shear bond strength according to various conditions and observing adhesive failure patterns. The specimens, 20 maxillary premolars and 80 porcelain teeth that were produced by duplication of the labial surface of a maxillary first premolar were used and randomly divided into four groups of twenty teeth each. The 5 different preparation procedures tested: (1) application of 37% phosphoric acid on natural teeth, (2) sandblasting on porcelain surfaces, (3) sandblasting and application of 9.6% hydrofluoric acid on porcelain surfaces, (4) sandblasting and application of silane on porcelain surface, (5) sandblasting and application of 9.6% hydrofluoric acid and silane on porcelain surfaces. The metal brackets were bonded with Transbond XT^(??) bonding material. The shear bond strength was tested by the micro universal testing machine(Kyung-Sung, Korea) and the amount of residual adhesive on the tooth surface after debonding was examined by stereoscope and assessed with an adhesive remnant index. The results of this study suggest that the direct bonding system on porcelain teeth with sandblasting, HF and porcelain primer is clinically useful.

코클리어 메카닉스의 신경동조모델

백승화,유선국,윤태성 明知大學校 産業技術硏究所 1987 産業技術硏究所論文集 Vol.6 No.2

A neural tuning model is analyzed which is based on the phenomena for basilar membrane motion, and the responses of tuning curves. By assuming the cochlear fluied is incompressible and non-viscous, a pair of second order differential equations for basilar membrane motion is solved and the neural tuning response for hair cell excitation is analyzed by linearly combining the pressure across the basilar membrane with its displacement. The sharply tuning filter mechanism with different characteristic place being sensitive to different characteristic frequency is obtained. The differences between the mechanical properties for basilar membrane and the neural tuning reponses are explained.

대호 간척기 토양의 염농도별 밭작물의 염해 평가

이승헌,류순호,설수일,안열,정영상,이상모 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts (ECe 1, 3, 9, 14, and 16 dS·m^(-1)) of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over 3 dS·m^(-1). Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were 14.2 dS·m^(-1) for radish, 11.4 dS·m^(-1) for Chinese cabbage, 10.2 dS·m^(-1) for tomato for red pepper, 8.9 dS·m^(-1) for buckwheat and green perilla, 8.6 dS·m^(-1) for soybean, and 8.9 dS·m^(-1) for tomato. At higher ECe that start the growth inhibition, increasing 1 dS·m^(-1) in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was 15.4~23.1 dS·m^(-1).

느타리버섯의 갈반병 원인균에 대한 길항세균 선발에 관한 연구

조재선,이은관,유승오,전억한 경희대학교 생명자원과학연구원 1997 硏究論文集 Vol.18 No.-

갈반병이 이병된 느타리 버섯과 버섯 재배장 주변 토양으로부터 채집한 균주로부터 갈반병 원인 균주 4개와 갈반병 길항 균주 3개를 선발 하였다. 선발된 균주를 API 20E kit에 의해 동정한 결과 갈반병 원인 균주 P-24, P-28은 P. putida로 P-27, P-33은 lane 1: PCR marker lane 2: P.fluorescens P. tolaasii로 동정 되었으며, 길항 균주중 A-11, A-20은 P. fluorescence로 A-29는 Pseudomonas sp.로 동정 되었으나, RAPD PCR 방법에 의한 유전적 유사성은 모든 균주가 다른 양상을 보였다(Fig. 5). 이는 생화학적 방법에 의한 Pseudomonas 계통의 균주 동정의 기술이 확립되지 않은 점도 있으며, 유전적 유사성은 달라도 같은 속의 균주가 많은 것으로 사료 된다. 길항성은 갈반병 원인 균주 대 길항 균주가 1:1의 농도로 길항성이 양호하여 Nair등(1972)의 보고 보다 우수한 길항성을 보였다. Screening experiments were carried out in order to select bacteria causing brown blotch disease on the mushroom. especially, Pleurotus ostreatus. Four brown blotch disease causing bacteria were isolated from the Pleurotus ostreatus and the soil around mushroom farm. Three other strains, A-11, A-20, A-29 showing antagonism against four brown blotch causing bacteria through pitting test and cross checking and found to be identified as Pseudomonas fluorescence for A-11 and A-20, and Pseudomonas sp. for A-29, respectively. Colony morphology test also showed that A-11 and A-29 were appeared as transparent gel with green color and A-20 colony showed opaque gel with light green color.

PDMS와 유리를 이용한 제한효 반응용 마이크로바이오칩 개발

허영선,염혜정,조철호,안유민,황승용 한양대학교 이학기술연구소 2005 이학기술연구지 Vol.8 No.-

최근 생화학 심험 분야에서 초미세 구조를 이용한 소형화 분야가 두드러지게 나타나고 있다. 소형화의 가증 큰 이점은 적은 양의 시료가 사용되기 때문에 실험 비용이 줄어들 뿐 아니라 실험 시간이 단축 된다는 것이다. 게다가 하나의 칩으로 바이오 실험의 모든 단계가 수행되는 랩 온어 칩의 통합을 위한 가능성을 나타내어 준다, 우리는 제한 효소 반응 실험에 응용하기 위해 새로운 마이크로바이오칩을 개발하였다, 이 마이크로 바이오칩은 PDMS와 유리를 사용해 제작하였고 총 실험 반응 양의 10㎕이다, 마이크로바이오칩을 이용한 제한효소 실험은 일반적으로 행해지는 실험 방법에 비해 반응 시간과 시료 양을 단축하는데 성공적이었다. In recent years, significant advances emerged in the area of miniaturzation of biochemical experiment using micro fabricated structures The key sdvantages of the miniaturization would be reduces assay costs due to the low consumpation of expensive reagent and increased experiment speed The additional benefits can be intergrated into Lab-on -a chip in which all the steps necessary in bio assays is performed in a single chip We have developed a novel Microbiochip for th application of Restriction Enzyme cut reaction The Microbiochip with reaction volum of 10 ㎕was fabricated using PDMS/glass The Restriction Enzyme cut reaciton using the microbiochip was successfully done with less time sample compared to the conventioal way

아미노산 투여가 운동중 싸이클선수의 젖산 및 심박수에 미치는 영향

이승국,유병렬,김문희,김완조,이선장 韓國體育大學校附屬 體育科學硏究所 1992 韓國體育大學校附屬 體育科學硏究所論文集 Vol.11 No.1

The purpose of the study was to determine whether a 15.6mg.Kg ??' does of glutamate given one hour prior to incremental exercise test would reduce the lactate formation. Five well trained cyclists served as subjects in this experiment which included a placebo and glutamate trial. The subjects initially work on treadmill 200m/min for 3 minutes and here increased the workload by 20m/min every 3minutes until exhaustion. Blood lactate were collected from finger-tip. Subjects in the glutamate trial showed significantly lower lactate formation during exercise then they did in the placebo trial. In both groups no significant difference was found on heart rate. During the recovery lactate and heart, rate were unaltered in either the glutamate or placebo trials. In summary, glutamate ingestion may reduce lactate formation resultly improvement of performance.

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

치아 우식증의 조기 진단을 위한 QLF-D 개발

박형주,김종수,유승훈,신지선 大韓小兒齒科學會 2011 大韓小兒齒科學會誌 Vol.38 No.4

QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion. 저자는 DSLR(digital single-lens reflex) 카메라를 이용한 QLF-D 시스템을 고안하여, 사람의 유구치에 자연 발생된 법 랑질 초기 우식증을 관찰하고, 유구치 법랑질 시편을 대상으로 2일부터 14일까지 인공 탈회시킨 후 탈회 기간에 따른 상관관 계를 편광 현미경 소견과 비교 분석한 결과 다음과 같은 결과를 얻었다. 1. 탈회 기간에 따른 편광 현미경 상의 병소 깊이와 QLF-D 영상의 광밀도 값 비율 간의 피어슨 상관 계수는 0.969였다 (p<0.01). 2. 편광현미경에서 측정된 병소 깊이의 탈회 기간에 대한 회귀분석 결과 y=8.67x-1.16의 회귀 방정식이 산출되었다 (p<0.05). 3. QLF-D 영상에서 측정된 광도값 비율의 탈회 기간에 대한 회귀분석 결과 y=3.53x+6.42의 회귀 방정식이 산출되었다 (p<0.05).