Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

Effect of treadmill exercise on skeletal muscle autophagy in rats with obesity induced by a high-fat diet Do Keun Cho1

( Do Keun Cho ),( Dong Hun Choi ),( Joon Yong Cho ) 한국운동영양학회 2017 Physical Activity and Nutrition (Phys Act Nutr) Vol.21 No.3

[Purpose] This study aimed to investigate the effects of treadmill exercise on body weight, blood biochemistry, and autophagy. [Methods] Triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, insulin, and glucose levels were measured, the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) score was calculated, and the soleus muscle was analyzed for autophagy- related factors (Beclin-1, p62, LC3, Lamp- 2) in rats with obesity induced by a high-fat diet. Eight-week-old Sprague Dawley rats were fed a high-fat diet for 35 weeks and then subjected to 10 weeks of treadmill exercise. The experimental group was divided into a Normal Diet-Sedentary (ND-SED, n=8) group, an (High-Fat Diet-Sedentary (HF-SED, n=8) group, and an High-Fat Diet + Treadmill Exercise (HF-TE, n=8) group. The intensity of treadmill exercise was set as 8 m/min for 5 min, 11 m/min for 5 min, 15 m/min for 20 min, and 11 m/min for the last 5 minutes. A glucose tolerance test was performed at the 2nd and 8th week of exercise by sampling of tail blood. [Results] With endurance exercise, the HFTE group showed a significant decrease in body weight, with improved blood biochemical indices and HOMA-IR scores, in comparison with the HF-SED group. However, there was no significant difference in Beclin-1, p62, LC3, and Lamp-2 proteins as measured by autophagic flux in the soleus muscle. [Conclusion] Treadmill exercise induced improvements in body weight, body fat, and biochemical indicators of obesity and Type 2 diabetes, but had no effect on autophagy in soleus muscle.

한우에 있어 황토 첨가급여가 면역기능에 미치는 영향

조원모,기광석,정하연,강수원,김준식,오성종,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.2

본 연구는 한우 육성우에 대한 황토 첨가급여가 항병성에 미치는 효과를 구명하고자 혈액성상변화를 규명한바 아래와 같은 결과를 얻었다. 대조구 및 처리구에서 전체적인 혈액성상의 변화는 없었으나 백혈구수치가 대조구 및 처리구에서 각각 15.35K/㎕ 및 13.37K/㎕이였으며, 적혈구에 있어서도 정상수치인 5~10M/㎕보다 대조구 10.03M/㎕, 처리구 11.35M/㎕로 모두 높은 수치를 보였다. 황토첨가에 따른 혈액성상의 변화는 처리간에 유의한 차이는 보이지 않았다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the immunity of growing Hanwoo steers. A total of 8 calves were divided into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitu reddish clay) for Hanwoo steers. This study was carried out for 5 months using 8 calves. The results obtained by the present study are summarized as follows : The proportion of WBC, NE as well as LY(15.35, 6.85, 6.86K/??) was higher in the control group than in the Reddish clay-supplemented group. But no statistical differences were found in blood substances among treatment calves.

순화슬러지와 생슬러지로 본 질산염제거특성

조순자,정용주,이나은,이정은,손형식,이상준 부산대학교 환경문제연구소 2003 環境硏究報 Vol.21 No.-

Using acclimated sludge with inorganic nitrogen medium for three months and non-acclimated sludge, raw sludge, under aerobic condition we investigated characteristics biological nitrogen removal. As results, e acclimated sludge under aerobic condition accomplished nitrification efficiently. But the samples from the reactor operated raw sludge had higher ammonium concentration rather than the concentration which was loaded by the medium. This means when we are trying to reorganize existing facilities, we should take high initial ammonium concentration into account.

[논문]도시하수처리장으로부터 분리한 호기성 탈질균의 특성

정용주,조순자,이상준 부산대학교 환경문제연구소 2004 環境硏究報 Vol.22 No.-

Characterization of an Aerobic Denitrifier Isolated from Municipal Sewage Treatment Plant

파킨슨씨병에서 Selegiline HCI (MAO-B^(�))의 효과 및 안전성

조기현,김명규,김태선,김병채,이승한,황인용,김준태,김종기 全南大醫大 2000 전남의대학술지 Vol.36 No.2

중심 정맥 도관 348례의 임상적 고찰

조남수,김용배,임경준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To evaluate clinical trends, central venous catheterization of 348 in total performed at the Chosun University Hospital from January 1993 through December 1994 were analyzed clinically & statistically according to sex, age, department, used veins and complications. The results are as follows : 1) Considering sexual distribution, male patients were 61.2% and female patients were 38.8%. 2) According to age categories, fourth decade of life was most frequent. 3) According to departmental distribution, general surgery was 38.5%, chest surgery was 21.0% and orthopedic surgery was 12.9%. 4) Subclavian vein used most frequently (74.1%), and femoral vein accounted for 14.9%, extermal jugular vein 7.5% internal jugular vein 3.4%. 5) Complications associated with subclavian vein catheterization using infraclavicular approach occupied 15.1%(39 cases) of total subclavian vein catheterization(258 cases), and among these complications pneumothorax occupied 43.6%, subcutaneous hematoma 28.2%, local infection 15.4%.

황토 급여가 거세한우의 발육에 미치는 영향

조원모,기광석,정하연,강수원,김준식,백봉현,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

본 연구는 거세한우 육성우에 대한 황토 첨가급여가 발육에 미치는 효과를 구명하고자 발육능력, 사료섭취량을 규명한 바 아래와 같은 결과를 얻었다. 전 시험기간 동안의 평균 일당증체량은 대조구와 황토급여구가 각각 0.91㎏과 0.87㎏/day로 대조구가 높은 것으로 나타났지만 유의성은 인정되지 않았다. 사료섭취량은 대조구와 황토급여구 모두 평균 일일 두당 농후사료 섭취량이 2.42㎏이었으나, 건초섭취량에 있어서는 각각 3.02와 3.14㎏으로 황토급여구가 다소 높은 경향으로 나타났다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the growth performance in growing Hanwoo steers. A total of 8 calves was allocated into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitim Reddish clay) for Hanwoo steers. This study was carried out for 5 months with a total of 8 calves. The results obtained in the present studies are summarized as follows : Average daily gains throughout the experimental period of 4 months to 9 months of age were 0.91 and 0.87㎏ for control group and reddish clay-supplemented group, respectively, however, no statistical significances were found. Average daily intake of concentrates was 2.42㎏ for each group, while that of hay tended to be higher in reddish clay-supplemented group(3.14㎏) than in control group(3.02㎏).

Molecular and Cytogenetic Analysis of Transgenic Plants of Rice(Oryza sativa L.) Produced by Agrobacterium-mediated Transformation

Cho,Joon-Hyeong,Kim,Yong-Wook 한국자원식물학회 2004 Plant Resources Vol.7 No.1

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ 수식 이미지 kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ 수식 이미지 proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.